##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660734_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 26785507 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.805754096795702 34.0 31.0 34.0 30.0 34.0 2 31.926257994668536 34.0 31.0 34.0 30.0 34.0 3 31.98514659438778 34.0 31.0 34.0 30.0 34.0 4 35.387226457949815 37.0 35.0 37.0 33.0 37.0 5 35.354003230179664 37.0 35.0 37.0 33.0 37.0 6 35.434661998370984 37.0 35.0 37.0 33.0 37.0 7 35.37460186958567 37.0 35.0 37.0 33.0 37.0 8 35.39194833982422 37.0 35.0 37.0 33.0 37.0 9 37.06540551948485 39.0 37.0 39.0 33.0 39.0 10-11 36.9899623143217 39.0 37.0 39.0 33.0 39.0 12-13 37.024946438385506 39.0 37.0 39.0 33.0 39.0 14-15 38.39503435570587 40.0 38.0 41.0 33.0 41.0 16-17 38.372998670512374 40.0 38.0 41.0 33.0 41.0 18-19 38.32937939162399 40.0 38.0 41.0 33.5 41.0 20-21 38.197191488665865 40.0 38.0 41.0 33.0 41.0 22-23 38.01334374592947 40.0 38.0 41.0 32.5 41.0 24-25 38.000682999952176 40.0 38.0 41.0 33.0 41.0 26-27 37.99305387424624 40.0 38.0 41.0 33.0 41.0 28-29 37.90941763767996 40.0 38.0 41.0 32.5 41.0 30-31 37.80636468818753 40.0 38.0 41.0 32.0 41.0 32-33 37.67005823335731 40.0 38.0 41.0 32.0 41.0 34-35 37.507271413604386 40.0 37.0 41.0 31.0 41.0 36-37 37.30433801383711 40.0 37.0 41.0 31.0 41.0 38-39 36.84331119810426 39.0 35.0 41.0 30.0 41.0 40-41 36.772125481888395 39.0 35.0 41.0 30.0 41.0 42-43 36.760818901057206 39.0 35.0 41.0 30.0 41.0 44-45 36.75075954321119 39.5 35.0 41.0 30.0 41.0 46-47 36.83019873396461 40.0 35.0 41.0 30.0 41.0 48-49 36.71404769377709 40.0 35.0 41.0 30.0 41.0 50-51 35.32870469840276 38.5 34.0 40.0 27.5 40.5 52-53 35.45739317161329 38.5 34.5 39.5 28.0 40.5 54-55 35.787973791199846 39.0 35.0 41.0 26.0 41.0 56-57 35.365061337087994 39.0 35.0 41.0 23.0 41.0 58-59 35.1509691416332 39.0 35.0 41.0 22.0 41.0 60-61 34.91580409510263 39.0 34.5 41.0 22.0 41.0 62-63 34.67859592876103 38.0 34.0 41.0 21.5 41.0 64-65 34.41511978847367 37.5 34.0 40.0 21.0 41.0 66-67 34.13675806099171 37.0 34.0 40.0 20.0 41.0 68-69 33.859980436435265 36.5 33.5 39.5 20.0 41.0 70-71 33.56163482363802 36.0 33.0 39.0 20.0 41.0 72-73 33.21654221441468 35.0 33.0 39.0 20.0 41.0 74-75 32.83400833144581 35.0 33.0 37.5 20.0 40.0 76-77 32.43532020879799 35.0 32.5 37.0 19.0 39.5 78-79 32.06077652366259 35.0 32.0 37.0 18.0 39.0 80-81 31.743874308595316 35.0 32.0 36.0 17.5 39.0 82-83 31.433836421315455 35.0 32.0 36.0 16.5 37.0 84-85 31.14931177147403 35.0 31.0 35.0 15.0 37.0 86-87 30.933383807146157 35.0 31.0 35.0 15.0 36.5 88-89 30.726556734580385 35.0 31.0 35.0 14.5 36.0 90-91 30.554987982867004 35.0 31.0 35.0 12.0 36.0 92-93 30.40349290383042 35.0 31.0 35.0 11.5 36.0 94-95 30.220242853719363 35.0 30.0 35.0 10.0 35.0 96-97 30.080202569994288 34.0 30.0 35.0 10.0 35.0 98-99 29.96771104612655 34.0 30.0 35.0 10.0 35.0 100 29.875571591756692 34.0 30.0 35.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5185313144621411 1101 2 0.5266402560250896 1101 3 0.5347571218230982 1101 4 0.45337646481627303 1101 5 0.4796489057979798 1101 6 0.44108394357491676 1101 7 0.49300483813389917 1101 8 0.4964683941686516 1101 9 0.5833687264252063 1101 10-11 0.5693068708550797 1101 12-13 0.5687458186323582 1101 14-15 0.7049241946203182 1101 16-17 0.6525283540125955 1101 18-19 0.6416257538014705 1101 20-21 0.6887505983796203 1101 22-23 0.7023173648728118 1101 24-25 0.7133529505489875 1101 26-27 0.6790084161163676 1101 28-29 0.6919751076969263 1101 30-31 0.6508781220528235 1101 32-33 0.6451361015895927 1101 34-35 0.6832890185518963 1101 36-37 0.6258810982589154 1101 38-39 0.6611517847998272 1101 40-41 0.6250495489595451 1101 42-43 0.6334651872208212 1101 44-45 0.6593294680136808 1101 46-47 0.7377593663399153 1101 48-49 0.73489881865396 1101 50-51 0.6782880058339416 1101 52-53 0.7139744074414551 1101 54-55 0.7428386767844515 1101 56-57 0.7242435663122748 1101 58-59 0.6555841264138635 1101 60-61 0.6834858029955271 1101 62-63 0.6436672689081675 1101 64-65 0.612136326073589 1101 66-67 0.5934393584598681 1101 68-69 0.5760816019142041 1101 70-71 0.5812272653557002 1101 72-73 0.5229988175020353 1101 74-75 0.5297673755373395 1101 76-77 0.4957355275558868 1101 78-79 0.5247133246727032 1101 80-81 0.454073631072049 1101 82-83 0.4987105347772136 1101 84-85 0.4971857362788086 1101 86-87 0.36568228297426586 1101 88-89 0.3873859291934103 1101 90-91 0.35804220551459665 1101 92-93 0.3362359651413662 1101 94-95 0.38687119586666796 1101 96-97 0.35766469863446915 1101 98-99 0.3371719202830832 1101 100 0.2515252252760156 1102 1 0.4162082971460883 1102 2 0.4579924509296731 1102 3 0.46414340395624976 1102 4 0.39009691706074534 1102 5 0.3812644468332138 1102 6 0.35914174297805346 1102 7 0.38729595836436204 1102 8 0.41223182856600005 1102 9 0.4705330593483339 1102 10-11 0.4818714791404588 1102 12-13 0.48090013510908847 1102 14-15 0.5601126400259631 1102 16-17 0.5245111459847394 1102 18-19 0.49888808684632835 1102 20-21 0.49763180252752903 1102 22-23 0.5543099266460132 1102 24-25 0.5265662497382024 1102 26-27 0.5277930446063905 1102 28-29 0.5453850563784357 1102 30-31 0.4969816854737772 1102 32-33 0.5153273849451239 1102 34-35 0.5052238501264625 1102 36-37 0.5334600868964188 1102 38-39 0.5336026184662757 1102 40-41 0.5297664701439899 1102 42-43 0.5461122070423841 1102 44-45 0.5563533634736757 1102 46-47 0.5825255598390839 1102 48-49 0.6250303658107654 1102 50-51 0.5525846047094305 1102 52-53 0.5792648070614774 1102 54-55 0.6365420163024211 1102 56-57 0.6162821093603696 1102 58-59 0.5208657620398114 1102 60-61 0.5638244947125699 1102 62-63 0.5176448409043317 1102 64-65 0.47120433893178415 1102 66-67 0.46385113456246785 1102 68-69 0.4512868896073954 1102 70-71 0.415495305203585 1102 72-73 0.3851450023149994 1102 74-75 0.4063944806805324 1102 76-77 0.4002763959895077 1102 78-79 0.38187374180058953 1102 80-81 0.32629855517598116 1102 82-83 0.32981056139737674 1102 84-85 0.3018558283005355 1102 86-87 0.27567256389037453 1102 88-89 0.23357256262766413 1102 90-91 0.21188377874098663 1102 92-93 0.2549154265485285 1102 94-95 0.2894778776295617 1102 96-97 0.2353873578289445 1102 98-99 0.20456142769678465 1102 100 0.211466549331206 1103 1 0.3913147003661628 1103 2 0.4377440237539787 1103 3 0.44809577102503795 1103 4 0.37950062510534366 1103 5 0.39277431547617425 1103 6 0.3668075810251139 1103 7 0.36571216493064895 1103 8 0.4155201179787724 1103 9 0.4501574332518743 1103 10-11 0.4400748155626317 1103 12-13 0.4406365612524823 1103 14-15 0.5275586216439052 1103 16-17 0.5101223511172677 1103 18-19 0.4962585850521748 1103 20-21 0.513780967004628 1103 22-23 0.5469318792164515 1103 24-25 0.5754629793955885 1103 26-27 0.5295570880019724 1103 28-29 0.533799849667048 1103 30-31 0.5167832693415946 1103 32-33 0.4803069092291352 1103 34-35 0.5164715731964762 1103 36-37 0.491761031872052 1103 38-39 0.5038011277070069 1103 40-41 0.48057089367595296 1103 42-43 0.5254333288077007 1103 44-45 0.4990120487229177 1103 46-47 0.5340171841019696 1103 48-49 0.5895291627436805 1103 50-51 0.5046734563363273 1103 52-53 0.4767138476488384 1103 54-55 0.5304176663244959 1103 56-57 0.5678148107124841 1103 58-59 0.5287715948653684 1103 60-61 0.5299710118843493 1103 62-63 0.4737695576356131 1103 64-65 0.46540735927795396 1103 66-67 0.467597603945201 1103 68-69 0.4607722288719813 1103 70-71 0.42301316015161916 1103 72-73 0.44338775141289943 1103 74-75 0.4363741531843388 1103 76-77 0.4708801708921868 1103 78-79 0.4233644789598472 1103 80-81 0.3738566163660906 1103 82-83 0.4149742493819808 1103 84-85 0.38959615682814075 1103 86-87 0.3334515298918248 1103 88-89 0.3740562461929322 1103 90-91 0.3308558672965347 1103 92-93 0.24645842132543905 1103 94-95 0.36168988113534795 1103 96-97 0.3458529866254487 1103 98-99 0.31479502649701274 1103 100 0.2926854406577455 1104 1 0.3190193634489411 1104 2 0.3468221882252607 1104 3 0.3342346063833901 1104 4 0.2746581682058533 1104 5 0.2856931508974583 1104 6 0.1938980776731114 1104 7 0.24210562582923956 1104 8 0.26376532783869777 1104 9 0.29246458209942716 1104 10-11 0.28406372226756815 1104 12-13 0.29693379754847626 1104 14-15 0.3699690181204929 1104 16-17 0.3652565475621188 1104 18-19 0.35211042781370594 1104 20-21 0.2794759027387741 1104 22-23 0.3245624536406808 1104 24-25 0.3619923516640924 1104 26-27 0.31284557405952995 1104 28-29 0.3370854932494325 1104 30-31 0.28688344224260476 1104 32-33 0.27403139981247904 1104 34-35 0.2706149265470046 1104 36-37 0.23962417081275333 1104 38-39 0.26849109038322183 1104 40-41 0.27439547025279154 1104 42-43 0.2613014097893256 1104 44-45 0.2726850196309343 1104 46-47 0.31813109678179075 1104 48-49 0.37581117823689425 1104 50-51 0.32307754662364374 1104 52-53 0.31188987507924537 1104 54-55 0.36827987775220095 1104 56-57 0.41770952525200755 1104 58-59 0.3739619979978741 1104 60-61 0.3654987375550789 1104 62-63 0.33505363718236225 1104 64-65 0.35149144290036816 1104 66-67 0.35631173380391346 1104 68-69 0.34164963068484155 1104 70-71 0.3253226375212819 1104 72-73 0.3072654434668962 1104 74-75 0.3099668919713565 1104 76-77 0.3163485806839077 1104 78-79 0.28512544103205784 1104 80-81 0.2725601509997091 1104 82-83 0.2776481599400604 1104 84-85 0.2798078980037708 1104 86-87 0.2364196238945908 1104 88-89 0.2121796971226253 1104 90-91 0.20301132806295996 1104 92-93 0.2308713932613493 1104 94-95 0.2964675649631019 1104 96-97 0.2545998012027155 1104 98-99 0.2332858295089899 1104 100 0.19093624706210832 1105 1 0.2829984300428343 1105 2 0.3340826540259343 1105 3 0.3331330623194475 1105 4 0.27415979953529046 1105 5 0.29049224220376857 1105 6 0.2679958259973745 1105 7 0.26965104797443473 1105 8 0.2539211086551134 1105 9 0.2727331578816319 1105 10-11 0.3140571932934364 1105 12-13 0.2931302951063728 1105 14-15 0.35085645655334474 1105 16-17 0.33072122531050496 1105 18-19 0.32690779781092516 1105 20-21 0.3460755171036567 1105 22-23 0.37027286185578134 1105 24-25 0.39489916644959067 1105 26-27 0.38485514054418246 1105 28-29 0.3832212691916155 1105 30-31 0.3798864942380007 1105 32-33 0.3620998191299307 1105 34-35 0.3864991077586595 1105 36-37 0.3582820948980512 1105 38-39 0.36152360198366296 1105 40-41 0.34184254339685793 1105 42-43 0.37341578815322407 1105 44-45 0.3936564145395991 1105 46-47 0.38079788785897506 1105 48-49 0.4360780556795376 1105 50-51 0.3311367232800748 1105 52-53 0.3685631471289099 1105 54-55 0.4516769445233848 1105 56-57 0.41955075877060466 1105 58-59 0.3198444175865589 1105 60-61 0.3111192528405766 1105 62-63 0.298162725474036 1105 64-65 0.2889075648256778 1105 66-67 0.27435636417973797 1105 68-69 0.30316176649987625 1105 70-71 0.3124767449833783 1105 72-73 0.29510859255124444 1105 74-75 0.3115530722032034 1105 76-77 0.3224064167630658 1105 78-79 0.32900882871648207 1105 80-81 0.29658607263954906 1105 82-83 0.27292670098622906 1105 84-85 0.252229221592021 1105 86-87 0.21961125392601488 1105 88-89 0.27441368818635326 1105 90-91 0.2594152049272971 1105 92-93 0.24955034384970176 1105 94-95 0.30233354680001057 1105 96-97 0.2666683459619783 1105 98-99 0.23457455656107484 1105 100 0.20798985435547124 1106 1 0.25581451107441566 1106 2 0.2897141891304855 1106 3 0.2831246889448309 1106 4 0.25891787475087824 1106 5 0.2894525502152092 1106 6 0.2407913399924837 1106 7 0.23497957824158533 1106 8 0.25420327318679625 1106 9 0.2660842001761097 1106 10-11 0.2424460350996398 1106 12-13 0.27119941919169577 1106 14-15 0.3209712660217008 1106 16-17 0.3112917245732376 1106 18-19 0.26365872491142994 1106 20-21 0.30595665422256246 1106 22-23 0.3113737923280553 1106 24-25 0.3337319422999556 1106 26-27 0.3391510782967231 1106 28-29 0.340442403664305 1106 30-31 0.30627693366789543 1106 32-33 0.3653912523433789 1106 34-35 0.3326014954090013 1106 36-37 0.26545211042603256 1106 38-39 0.3600690544777194 1106 40-41 0.3470674570934378 1106 42-43 0.34146270854876093 1106 44-45 0.38226484279530126 1106 46-47 0.3939786281945672 1106 48-49 0.3886822529969862 1106 50-51 0.29150684699632023 1106 52-53 0.34819128750527284 1106 54-55 0.4668289305238602 1106 56-57 0.5070560250394252 1106 58-59 0.441569036896432 1106 60-61 0.4249408513333819 1106 62-63 0.44224316520504914 1106 64-65 0.42571187160909574 1106 66-67 0.4215380583536472 1106 68-69 0.4030284030622724 1106 70-71 0.39213800792647646 1106 72-73 0.36354495323077174 1106 74-75 0.38794796966646317 1106 76-77 0.368653823241857 1106 78-79 0.40330227419692477 1106 80-81 0.3249415345699944 1106 82-83 0.3644745902541011 1106 84-85 0.34548359793087613 1106 86-87 0.3134559930759302 1106 88-89 0.328202470318967 1106 90-91 0.30195393085093514 1106 92-93 0.2949811832746825 1106 94-95 0.3325931838392968 1106 96-97 0.3336295898885311 1106 98-99 0.33020553973336675 1106 100 0.3145524848875709 1107 1 0.18235263066712193 1107 2 0.20625499117516455 1107 3 0.2239603540968247 1107 4 0.22737495843497157 1107 5 0.21953663848039184 1107 6 0.17600864362132995 1107 7 0.20291343845529752 1107 8 0.23604276652230283 1107 9 0.24139460403831947 1107 10-11 0.23430885487800168 1107 12-13 0.22967601722681508 1107 14-15 0.2518326498571355 1107 16-17 0.26151073459140406 1107 18-19 0.24922631028911724 1107 20-21 0.21132108849459286 1107 22-23 0.24073135639108045 1107 24-25 0.2535380147139037 1107 26-27 0.25296005826568546 1107 28-29 0.28338108544632945 1107 30-31 0.24439476652428738 1107 32-33 0.1718764234757444 1107 34-35 0.24930025482707663 1107 36-37 0.2112344332276237 1107 38-39 0.1969227072802724 1107 40-41 0.21357604403603148 1107 42-43 0.24375179585102558 1107 44-45 0.24858699562441444 1107 46-47 0.23972508294146166 1107 48-49 0.25688227775237493 1107 50-51 0.18610443415497713 1107 52-53 0.1889925435060178 1107 54-55 0.21052260436001546 1107 56-57 0.2158685642398197 1107 58-59 0.1437103737333274 1107 60-61 0.1643502562150232 1107 62-63 0.12378332696402339 1107 64-65 0.13880466482834208 1107 66-67 0.1704805647847607 1107 68-69 0.14370726018739077 1107 70-71 0.1068715648043721 1107 72-73 0.1087304367516424 1107 74-75 0.11022397758325297 1107 76-77 0.08367186212833388 1107 78-79 0.11721443856772851 1107 80-81 0.06382559255109399 1107 82-83 0.09808063757101237 1107 84-85 0.10140274042094788 1107 86-87 0.07790280959602214 1107 88-89 0.07633272304758876 1107 90-91 0.0940589974868331 1107 92-93 0.0748298338937623 1107 94-95 0.10930705505299088 1107 96-97 0.15317057489123798 1107 98-99 0.10113900698195621 1107 100 0.08939415452513089 1108 1 0.08933075336606322 1108 2 0.11401499697566209 1108 3 0.13309181594760133 1108 4 0.10625728813843693 1108 5 0.11486249699797924 1108 6 0.07638427807382442 1108 7 0.11177398571343389 1108 8 0.11118321158980393 1108 9 0.11141639922363566 1108 10-11 0.07534814789651989 1108 12-13 0.06024146201202285 1108 14-15 0.1256680038235629 1108 16-17 0.09248991835993792 1108 18-19 0.09307641240624775 1108 20-21 0.07378847571205682 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-1.211602944436006 2316 88-89 -1.1968304823410314 2316 90-91 -1.104639281560818 2316 92-93 -0.942811726063578 2316 94-95 -1.1727297733039386 2316 96-97 -1.3435537970166678 2316 98-99 -1.2688387934250258 2316 100 -1.019570661493301 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 13921.0 3 3410.0 4 2471.0 5 2086.0 6 3976.0 7 21180.0 8 40892.0 9 47698.0 10 48236.0 11 44516.0 12 42445.0 13 43411.0 14 49512.0 15 59999.0 16 76957.0 17 96076.0 18 103244.0 19 114078.0 20 133248.0 21 164089.0 22 213559.0 23 321857.0 24 458233.0 25 527242.0 26 517377.0 27 445534.0 28 394387.0 29 386433.0 30 428600.0 31 512638.0 32 636679.0 33 823975.0 34 1137080.0 35 1721021.0 36 2967116.0 37 5451417.0 38 6770541.0 39 1956450.0 40 3923.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 16.660946121989852 33.29211567507331 27.88779586107941 22.159142341857425 2 21.163169715200052 34.921369934722044 25.29977403206604 18.615686318011864 3 26.8147386635338 24.855744050752154 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26.481053592952144 29.18838173790055 20.34298305463014 24-25 20.470226024506154 29.896586806509305 25.609555558374836 24.0236316106097 26-27 20.446394637645867 26.482867536076494 32.748348861071086 20.322388965206557 28-29 20.482470327241323 26.540427209695157 32.73433080782294 20.24277165524058 30-31 20.396084262041906 26.404031637020818 32.818524536016945 20.381359564920327 32-33 20.525462510943203 26.552006495381196 32.58857010984781 20.33396088382779 34-35 20.537788431128067 26.712597327184245 32.429778906646725 20.319835335040963 36-37 20.404793730141026 26.65783927264027 32.580365791352975 20.357001205865725 38-39 20.432431971858335 26.590669580096144 32.599073191890156 20.37782525615537 40-41 20.52484839631193 26.61332478571678 32.502780955759334 20.35904586221195 42-43 20.546065284428327 26.666148893253727 32.4947533414877 20.29303248083024 44-45 20.641645141302302 26.74070966120816 32.397480899142195 20.22016429834734 46-47 20.546193865434315 26.85178878032269 32.3700969824506 20.231920371792402 48-49 20.656979365315728 26.845110857969633 32.20190239745575 20.296007379258892 50-51 20.71154209870344 26.879603566754177 32.12328095732401 20.28557337721837 52-53 20.8237039812019 27.042616082355377 31.881576216220903 20.25210372022182 54-55 21.199975146586088 27.31323476992606 31.05909646628368 20.427693617204167 56-57 21.742950686253824 27.9235255983189 29.795072400135176 20.538451315292107 58-59 21.939231115152026 28.49447529685603 29.00915437877771 20.55713920921423 60-61 22.184054248660097 28.815133697233186 28.50239840648513 20.49841364762159 62-63 22.282463046861423 29.04238130661102 28.23914747145603 20.436008175071528 64-65 22.428235337562874 29.080348534975244 28.16756751695714 20.323848610504747 66-67 22.483938009728046 28.883678172503103 28.429019099149965 20.203364718618886 68-69 22.673337220070096 28.68588547843272 28.58046808611808 20.060309215379096 70-71 22.803334967094965 28.507207830721736 28.705681552326624 19.98377564985668 72-73 23.011199014271604 28.3753917522654 28.69864980199783 19.914759431465164 74-75 23.313989379974505 28.43853982890794 28.50588722542219 19.741583565695368 76-77 23.700930172503643 28.51316392440473 28.15789267621583 19.628013226875797 78-79 24.013312636034396 28.65661874398761 27.784548428786614 19.545520191191372 80-81 24.35119931576055 28.82409518507784 27.373541557698694 19.45116394146292 82-83 24.515663798688422 28.97032273033781 27.052429711011754 19.46158375996201 84-85 24.69589069533239 28.952534187984114 26.896152226932923 19.45542288975057 86-87 24.873786565280227 28.928492685691655 26.816250315225183 19.381470433802935 88-89 25.12120295676501 28.87034326305851 26.689266706433195 19.319187073743286 90-91 25.314654275110726 28.834022711338804 26.5715833241609 19.279739689389572 92-93 25.46802489243514 28.862667091860743 26.47274499162084 19.19656302408328 94-95 25.61775253300067 28.87812820711002 26.32453941682783 19.179579843061482 96-97 25.748020637496104 28.86499229248973 26.210592746342392 19.17639432367177 98-99 25.903856215695598 28.87674231414808 26.11666393601429 19.102737534142033 100 25.98014117211535 28.90657209640539 26.00552108397865 19.10776564750061 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 31128.0 1 22398.0 2 11972.5 3 9712.0 4 9137.5 5 9090.0 6 9316.5 7 9526.0 8 9451.5 9 9520.5 10 10132.5 11 13050.0 12 110225.0 13 137993.0 14 70023.5 15 74001.5 16 83601.5 17 91943.5 18 99461.0 19 107303.5 20 116976.0 21 132420.0 22 154829.0 23 178556.5 24 201855.0 25 230754.5 26 263346.0 27 300463.0 28 348396.0 29 396703.5 30 434637.5 31 472163.5 32 513434.5 33 549001.5 34 583345.5 35 627590.5 36 670638.0 37 706453.0 38 751013.0 39 782884.5 40 798406.0 41 829989.0 42 868616.5 43 876579.0 44 863759.0 45 867863.0 46 882836.5 47 890654.0 48 889006.5 49 880709.5 50 872634.0 51 866723.0 52 850454.0 53 809097.5 54 755346.0 55 705727.5 56 654799.0 57 595655.0 58 529012.0 59 454493.0 60 374938.0 61 304975.0 62 242446.0 63 184665.0 64 138793.5 65 103734.0 66 77573.5 67 59632.5 68 46241.5 69 35953.5 70 27959.5 71 22309.0 72 18389.0 73 14942.5 74 12262.0 75 10481.5 76 8349.5 77 5898.5 78 4265.5 79 3322.0 80 2697.5 81 2193.5 82 1852.5 83 1572.5 84 1321.0 85 1119.0 86 983.0 87 851.5 88 707.0 89 642.5 90 566.5 91 475.0 92 406.5 93 353.0 94 299.5 95 269.5 96 240.0 97 194.5 98 155.5 99 121.5 100 81.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3987678859317466 2 0.38108294907391527 3 0.16935277723135875 4 0.17591976138439344 5 0.27268477688326004 6 0.10288772954717638 7 0.1374735971956775 8 0.14315577450148695 9 0.13608478644813404 10-11 0.13268556014265476 12-13 0.13449997418380022 14-15 0.14056108775540443 16-17 0.14476485362028055 18-19 0.15630094289423008 20-21 0.1366149238840243 22-23 0.09943436948944069 24-25 0.09457913191637551 26-27 0.08895855508727164 28-29 0.0989098320968873 30-31 0.10356720147204979 32-33 0.10465360987940231 34-35 0.10507361312966747 36-37 0.10131598405062857 38-39 0.10040317698671898 40-41 0.1014373183229274 42-43 0.06362022566905305 44-45 0.06668158269320792 46-47 0.08523265958714166 48-49 0.0896454937365942 50-51 0.0989340989513471 52-53 0.10419440632577909 54-55 0.10684696018634257 56-57 0.10747976508340873 58-59 0.10692162743083415 60-61 0.10473387716723077 62-63 0.10934457951458601 64-65 0.1159638307387648 66-67 0.12459723088310405 68-69 0.12955886927956975 70-71 0.12827272599320222 72-73 0.11526942536499309 74-75 0.1127624726311882 76-77 0.11475795474022575 78-79 0.11321420946036227 80-81 0.10749096517008246 82-83 0.11054112210756363 84-85 0.11643423437906178 86-87 0.12987993843088355 88-89 0.13769199888581538 90-91 0.1444475178311913 92-93 0.1437549791385319 94-95 0.1338765026922955 96-97 0.13057434380465527 98-99 0.12565750575488452 100 0.11971025973112999 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2.6785507E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 36.48537134959862 #Duplication Level Percentage of deduplicated Percentage of total 1 65.83808437488324 24.021269573638214 2 16.996048368679414 12.402142724140166 3 6.534504017237626 7.152414169630764 4 3.095615200451956 4.517786805758071 5 1.7821422745206397 3.2511061341851937 6 1.1117893925417657 2.43384293096586 7 0.7889707567093568 2.0150123729760296 8 0.5553383235343251 1.6209379967050717 9 0.44470454785748836 1.4602689512492235 >10 2.4515086247259394 16.416936265548426 >50 0.2139773941034325 5.512956754252967 >100 0.18300180484700163 11.463286905471987 >500 0.0035060173417624206 0.7572371277052403 >1k 7.373456486512552E-4 0.5583974364360236 >5k 3.066725034269384E-5 0.06947660428356672 >10k+ 4.0889667122587344E-5 6.346927247053355 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 1619349 6.045616385009998 No Hit AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 31094 0.11608516501106364 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 28800 0.1075208320678791 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.09825836038869826 1.4933448898316541E-5 0.0 0.0 5.9733795593266166E-5 2 0.11091819169224611 3.7333622245791355E-5 0.0 0.0 5.9733795593266166E-5 3 0.12513483504344344 4.106698447037049E-5 0.0 0.0 5.9733795593266166E-5 4 0.1281513917209034 5.9733795593266166E-5 0.0 0.0 6.720052004242444E-5 5 0.12853592803003505 6.346715781784529E-5 0.0 0.0 7.840060671616184E-5 6 0.1286703290701199 6.346715781784529E-5 0.0 0.0 8.213396894074098E-5 7 0.12887193063024718 8.213396894074098E-5 0.0 0.0 1.0080078006363665E-4 8 0.1293460676327687 9.706741783905751E-5 0.0 0.0 1.0453414228821578E-4 9 0.12961113635071383 9.706741783905751E-5 0.0 3.7333622245791354E-6 1.0453414228821578E-4 10-11 0.12973620398523722 1.1946759118653233E-4 0.0 5.600043336868703E-6 1.157342289619532E-4 12-13 0.1298164712730657 1.4746780787087586E-4 0.0 1.1200086673737406E-5 1.2506763452340103E-4 14-15 0.13003860632542816 1.4933448898316542E-4 0.0 1.8666811122895677E-5 1.3626772119713844E-4 16-17 0.13029060827558725 1.5680121343232366E-4 0.0 2.240017334747481E-5 1.456011267585863E-4 18-19 0.130527676776848 1.624012567691924E-4 0.0 2.240017334747481E-5 1.5306785120774454E-4 20-21 0.13075354519143506 1.904014734535359E-4 0.0 2.8000216684343516E-5 1.5680121343232369E-4 22-23 0.13113061477611754 2.1653500902558986E-4 0.0 3.360026002121222E-5 1.7546802455521937E-4 24-25 0.1321404892578662 2.594686746082499E-4 0.0 3.360026002121222E-5 2.07201603464142E-4 26-27 0.135491181854426 2.968022968540412E-4 0.0 3.546694113350179E-5 2.1093496568872112E-4 28-29 0.14430191670443274 3.2106915131380567E-4 0.0 3.920030335808092E-5 2.1653500902558986E-4 30-31 0.16332153055755114 3.397359624367013E-4 0.0 4.106698447037049E-5 2.3333513903619596E-4 32-33 0.19742766116019383 3.4346932466128045E-4 0.0 4.106698447037049E-5 2.594686746082499E-4 34-35 0.2551827747744331 3.528027302227283E-4 0.0 4.106698447037049E-5 2.706687612819873E-4 36-37 0.3433293235778587 3.752029035702031E-4 0.0 4.106698447037049E-5 2.930689346294621E-4 38-39 0.46723774913052796 3.826696280193614E-4 0.0 4.106698447037049E-5 2.9866897796633084E-4 40-41 0.62436376507639 3.864029902439405E-4 0.0 4.106698447037049E-5 3.173357890892265E-4 42-43 0.8177929952940596 4.106698447037049E-4 0.0 4.106698447037049E-5 3.4346932466128045E-4 44-45 1.0510628004913254 4.2373661248973186E-4 0.0 4.106698447037049E-5 3.7146954134562396E-4 46-47 1.338675426229565 4.442701047249171E-4 0.0 4.2933665582660056E-5 3.938697146930988E-4 48-49 1.6830855581714395 4.666702780723919E-4 0.0 4.480034669494962E-5 4.106698447037049E-4 50-51 2.072402437631664 4.7600368363383977E-4 1.8666811122895677E-6 4.480034669494962E-5 4.2186993137744225E-4 52-53 2.489956602277493 4.946704947567354E-4 3.7333622245791354E-6 4.480034669494962E-5 4.330700180511797E-4 54-55 2.9493916243586504 5.077372625427623E-4 3.7333622245791354E-6 5.0400390031818324E-5 4.386700613880484E-4 56-57 3.4667460279919284 5.170706681042103E-4 3.7333622245791354E-6 5.4133752256397465E-5 4.5733687251094406E-4 58-59 4.044967302653633 5.376041603393955E-4 3.7333622245791354E-6 5.600043336868703E-5 4.7787036474612927E-4 60-61 4.671686819293733 5.39470841451685E-4 3.7333622245791354E-6 5.600043336868703E-5 5.002705380936041E-4 62-63 5.324875874106098 5.469375659008433E-4 3.7333622245791354E-6 5.600043336868703E-5 5.170706681042103E-4 64-65 6.001913646809075 5.581376525745807E-4 3.7333622245791354E-6 5.600043336868703E-5 5.338707981148163E-4 66-67 6.718375724603607 5.693377392483182E-4 3.7333622245791354E-6 5.78671144809766E-5 5.562709714622911E-4 68-69 7.4790968115705265 5.749377825851868E-4 3.7333622245791354E-6 5.9733795593266166E-5 5.693377392483182E-4 70-71 8.272593085507024 5.842711881466347E-4 3.7333622245791354E-6 5.9733795593266166E-5 5.712044203606077E-4 72-73 9.08706525510232 5.936045937080824E-4 3.7333622245791354E-6 5.9733795593266166E-5 5.861378692589242E-4 74-75 9.919961567275916 6.178714481678469E-4 3.7333622245791354E-6 5.9733795593266166E-5 5.936045937080824E-4 76-77 10.772194829091717 6.290715348415844E-4 3.7333622245791354E-6 5.9733795593266166E-5 6.08538042606399E-4 78-79 11.648205874915863 6.608051137505069E-4 5.600043336868703E-6 5.9733795593266166E-5 6.122714048309782E-4 80-81 12.547731502711521 6.682718381996652E-4 7.466724449158271E-6 5.9733795593266166E-5 6.160047670555573E-4 82-83 13.461666042012943 6.832052870979818E-4 7.466724449158271E-6 5.9733795593266166E-5 6.234714915047156E-4 84-85 14.38374117764506 6.962720548840087E-4 7.466724449158271E-6 5.9733795593266166E-5 6.272048537292947E-4 86-87 15.314522513984894 7.018720982208775E-4 7.466724449158271E-6 5.9733795593266166E-5 6.290715348415844E-4 88 16.01865142967053 7.093388226700357E-4 7.466724449158271E-6 5.9733795593266166E-5 6.384049404030321E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 207065 0.0 85.09326 8 AGTGGTA 208275 0.0 84.83099 5 GTGGTAT 208795 0.0 84.55697 6 TGGTATC 208400 0.0 84.35325 7 AAGCAGT 211750 0.0 83.950294 1 GTATCAA 211000 0.0 83.42431 9 GCAGTGG 215000 0.0 82.68778 3 CAGTGGT 217135 0.0 81.265686 4 AGCAGTG 220055 0.0 80.829155 2 CAACGCA 205635 0.0 43.955536 12-13 ATCAACG 207205 0.0 43.740967 10-11 ACGCAGA 206905 0.0 43.718353 14-15 GAGTACT 198370 0.0 43.560986 20-21 AGAGTAC 205095 0.0 43.481335 18-19 TCAACGC 205040 0.0 43.007805 12-13 AGTACTT 206015 0.0 42.91651 20-21 AACGCAG 206270 0.0 42.80901 14-15 CGCAGAG 207965 0.0 42.39624 16-17 TATCAAC 209275 0.0 41.989914 10-11 GTACTTT 207355 0.0 41.582825 22-23 >>END_MODULE