FastQCFastQC Report
Thu 26 May 2016
SRR1660734_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660734_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26785507
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT14951885.582078397844028No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT340220.12701644960463135No Hit
TAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT323050.12060626666502897No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1946800.084.146611
GGTATCA2021150.083.765758
AGTGGTA2012750.083.56355
GTGGTAT2016450.083.3752446
GCAGTGG2023650.083.193033
TGGTATC2036750.083.0466467
GTATCAA2060800.082.024119
AGCAGTG2075750.080.7845152
CAGTGGT2092800.080.220184
CAACGCA2001550.043.3962412-13
GAGTACT1939450.043.10630820-21
ACGCAGA2020550.043.0927814-15
ATCAACG2021600.043.07728610-11
AGAGTAC2023200.042.91962418-19
AGTACTT2020050.042.60860420-21
TCAACGC1995550.042.25662212-13
AACGCAG2013050.041.97445714-15
CGCAGAG2035150.041.51311516-17
TATCAAC2053100.041.11671410-11
GTACTTT2038050.041.01933722-23
Produced by FastQC (version 0.11.5)