##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660734_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 26785507 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.161662573719436 34.0 31.0 34.0 27.0 34.0 2 31.820894299294018 34.0 31.0 34.0 28.0 34.0 3 31.59828096589697 34.0 31.0 34.0 30.0 34.0 4 35.53550817611927 37.0 35.0 37.0 33.0 37.0 5 35.874057825375495 37.0 35.0 37.0 33.0 37.0 6 35.340810312084066 37.0 35.0 37.0 33.0 37.0 7 35.54232305552402 37.0 35.0 37.0 33.0 37.0 8 35.91479366061654 37.0 35.0 37.0 33.0 37.0 9 37.71827440115283 39.0 37.0 39.0 35.0 39.0 10-11 37.73035180928254 39.0 37.5 39.0 35.0 39.0 12-13 37.810977219882375 39.0 38.0 39.0 35.0 39.0 14-15 39.25177643641392 41.0 39.0 41.0 36.0 41.0 16-17 39.22014427428982 41.0 39.0 41.0 36.0 41.0 18-19 39.2120685824614 41.0 39.0 41.0 36.0 41.0 20-21 39.14401129312206 40.5 39.0 41.0 36.0 41.0 22-23 39.01519704293818 40.0 38.5 41.0 35.5 41.0 24-25 39.026269336622974 40.0 39.0 41.0 35.0 41.0 26-27 38.98251084065723 40.0 39.0 41.0 35.0 41.0 28-29 38.901482693607406 40.0 38.0 41.0 35.0 41.0 30-31 38.79673309898521 40.0 38.0 41.0 35.0 41.0 32-33 38.66443336316165 40.0 38.0 41.0 34.5 41.0 34-35 38.566885741606455 40.0 38.0 41.0 34.0 41.0 36-37 38.42258472090896 40.0 38.0 41.0 34.0 41.0 38-39 38.000610722059506 40.0 37.0 41.0 33.5 41.0 40-41 37.86029889223303 40.0 36.5 41.0 33.0 41.0 42-43 37.68317043616162 40.0 36.0 41.0 33.0 41.0 44-45 37.541696504008684 40.0 36.0 41.0 33.0 41.0 46-47 37.373856541151156 40.0 35.0 41.0 33.0 41.0 48-49 37.407507462897755 40.0 35.5 41.0 33.0 41.0 50-51 37.392177810933354 40.0 35.5 41.0 33.0 41.0 52-53 37.12186552974338 40.0 35.0 41.0 31.5 41.0 54-55 36.56960667199617 39.0 35.0 41.0 30.0 41.0 56-57 36.04460316916906 39.0 35.0 41.0 27.5 41.0 58-59 35.892882259051504 39.0 35.0 41.0 27.0 41.0 60-61 35.739297953180426 39.0 35.0 41.0 27.0 41.0 62-63 35.49914621739286 38.5 35.0 41.0 26.5 41.0 64-65 35.23796833489095 38.0 35.0 40.0 26.0 41.0 66-67 34.95734075520765 37.0 35.0 40.0 26.0 41.0 68-69 34.696542107640525 37.0 34.0 40.0 26.0 41.0 70-71 34.40114178163587 36.0 34.0 39.0 26.0 41.0 72-73 34.050084454253565 36.0 34.0 39.0 26.0 41.0 74-75 33.65885682133999 35.0 34.0 38.5 25.0 40.0 76-77 31.950791821860978 34.5 31.0 36.0 22.0 39.0 78-79 32.88046761631206 35.0 33.0 37.0 24.0 39.0 80-81 32.783997387094445 35.0 34.0 36.5 24.0 39.0 82-83 32.531566417615316 35.0 33.0 36.0 24.0 37.5 84-85 32.28702303450892 35.0 33.0 36.0 23.0 37.0 86-87 32.073525862325475 35.0 33.0 35.0 23.0 37.0 88-89 31.89784218010135 35.0 33.0 35.0 22.0 36.0 90-91 31.736094112386972 35.0 33.0 35.0 20.0 36.0 92-93 31.589882394236554 35.0 33.0 35.0 20.0 36.0 94-95 31.47787322076823 35.0 33.0 35.0 20.0 35.5 96-97 31.371592256961947 35.0 33.0 35.0 19.0 35.0 98-99 31.26994751303382 35.0 33.0 35.0 19.0 35.0 100 31.20191135452467 35.0 33.0 35.0 19.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.6038882284075946 1101 2 1.2249070464854661 1101 3 1.5592086530762579 1101 4 0.9764099175147862 1101 5 0.6110174152909806 1101 6 1.1347656412816036 1101 7 0.8528044798731145 1101 8 0.4952066070634089 1101 9 0.5289558216881645 1101 10-11 0.4448350276798081 1101 12-13 0.40056752513059024 1101 14-15 0.49372423874884674 1101 16-17 0.5417790470453028 1101 18-19 0.4992856637830414 1101 20-21 0.5136603451194972 1101 22-23 0.509781531606663 1101 24-25 0.5089153971477671 1101 26-27 0.4853769645480952 1101 28-29 0.48224043023378727 1101 30-31 0.4883133712424268 1101 32-33 0.5379763446558528 1101 34-35 0.528288469015024 1101 36-37 0.513423329192392 1101 38-39 0.5450716258266297 1101 40-41 0.5420500193060889 1101 42-43 0.5652364646780512 1101 44-45 0.524458854766408 1101 46-47 0.5340444481031312 1101 48-49 0.5102746238028075 1101 50-51 0.4895113474518382 1101 52-53 0.5035916420301305 1101 54-55 0.525284010176037 1101 56-57 0.5924823623091271 1101 58-59 0.5657978111185713 1101 60-61 0.5987787733675987 1101 62-63 0.5389205472407355 1101 64-65 0.5200889109616753 1101 66-67 0.5418885902599584 1101 68-69 0.4821252216962719 1101 70-71 0.47655075214013465 1101 72-73 0.46381618848878503 1101 74-75 0.4883317295063989 1101 76-77 0.406735460685848 1101 78-79 0.4975691000447924 1101 80-81 0.5004231168995688 1101 82-83 0.48716823207350046 1101 84-85 0.4801017792233111 1101 86-87 0.4727350146573812 1101 88-89 0.44587044604773496 1101 90-91 0.44761188197485424 1101 92-93 0.41209847835166613 1101 94-95 0.3896384623584588 1101 96-97 0.32995168520868745 1101 98-99 0.28634205832671356 1101 100 0.3291614615543317 1102 1 1.5133295595358796 1102 2 1.1517474368869962 1102 3 1.5062151755065365 1102 4 0.9060741269806627 1102 5 0.4973464031738857 1102 6 1.0574564741907224 1102 7 0.7712796691799042 1102 8 0.4239098763502227 1102 9 0.4530713972237592 1102 10-11 0.3725202826820819 1102 12-13 0.34725779397570733 1102 14-15 0.4667394165331231 1102 16-17 0.4736008957115416 1102 18-19 0.41266491593366794 1102 20-21 0.39415167952402186 1102 22-23 0.4205069065838103 1102 24-25 0.4272689537138348 1102 26-27 0.4171071435859304 1102 28-29 0.44513203268329704 1102 30-31 0.43572221742751793 1102 32-33 0.4606187953575116 1102 34-35 0.40302791346235267 1102 36-37 0.3870721730667128 1102 38-39 0.40985300052069107 1102 40-41 0.4211829211724023 1102 42-43 0.41967844984235114 1102 44-45 0.39421692073531034 1102 46-47 0.4143269885439338 1102 48-49 0.3891813161870843 1102 50-51 0.37010602527482206 1102 52-53 0.37753039944338695 1102 54-55 0.42651606759243776 1102 56-57 0.45539944215175865 1102 58-59 0.4295148685702941 1102 60-61 0.4411441592742875 1102 62-63 0.39583283480798315 1102 64-65 0.3357622585750448 1102 66-67 0.3590054975650574 1102 68-69 0.3192438042056196 1102 70-71 0.30507458266178844 1102 72-73 0.2877746884881205 1102 74-75 0.3072056075820271 1102 76-77 0.15885384599812014 1102 78-79 0.32700932885354206 1102 80-81 0.35328025529086915 1102 82-83 0.3423084423476084 1102 84-85 0.34279812311492464 1102 86-87 0.34234210672169496 1102 88-89 0.3489144614002413 1102 90-91 0.35075297800354477 1102 92-93 0.3095281168411077 1102 94-95 0.27487873858448 1102 96-97 0.2713658469825546 1102 98-99 0.28353862609862546 1102 100 0.2909271036899348 1103 1 1.507351376397228 1103 2 1.1181495702431512 1103 3 1.4416315370738992 1103 4 0.8913775487043338 1103 5 0.5048834220114031 1103 6 1.038504737140947 1103 7 0.7669621605513441 1103 8 0.4297461743564668 1103 9 0.4497460626862022 1103 10-11 0.3671492781958321 1103 12-13 0.3280552355100852 1103 14-15 0.38789346311713757 1103 16-17 0.4213888935634458 1103 18-19 0.3831518446139981 1103 20-21 0.40439394731576783 1103 22-23 0.40505920566620546 1103 24-25 0.3796153606909769 1103 26-27 0.40716455061348 1103 28-29 0.4145293198468565 1103 30-31 0.42802047667536414 1103 32-33 0.43971146176584597 1103 34-35 0.4113600635561454 1103 36-37 0.35580769999807416 1103 38-39 0.35406959342027733 1103 40-41 0.42870867659131306 1103 42-43 0.44129310647220166 1103 44-45 0.420350610079133 1103 46-47 0.4400630256179454 1103 48-49 0.3754054111772902 1103 50-51 0.3683352381194922 1103 52-53 0.3721336230763086 1103 54-55 0.4090660113144722 1103 56-57 0.44353919186863067 1103 58-59 0.4360214346645819 1103 60-61 0.42024414109882 1103 62-63 0.38056039572092004 1103 64-65 0.3817998046995541 1103 66-67 0.4048952142232096 1103 68-69 0.353279214021363 1103 70-71 0.33335411849574825 1103 72-73 0.3392034632324368 1103 74-75 0.3301269824425219 1103 76-77 0.1961672071769094 1103 78-79 0.34055641318213503 1103 80-81 0.3684915083796483 1103 82-83 0.3880991516804073 1103 84-85 0.35850159920885005 1103 86-87 0.3629343611390041 1103 88-89 0.3279238026275948 1103 90-91 0.34510180647456323 1103 92-93 0.37571730155756455 1103 94-95 0.33593794882729355 1103 96-97 0.31285109534661615 1103 98-99 0.3390479364333068 1103 100 0.32068495919734374 1104 1 1.4152834404912191 1104 2 1.0571723641370276 1104 3 1.4158820533869623 1104 4 0.8789931975116545 1104 5 0.46460343595856557 1104 6 0.9867683624041206 1104 7 0.7385943032067388 1104 8 0.3981196297439169 1104 9 0.40084390691526295 1104 10-11 0.32115368440695846 1104 12-13 0.2818042460005685 1104 14-15 0.33624511970178617 1104 16-17 0.34245173953590324 1104 18-19 0.30983015239277734 1104 20-21 0.315651355245123 1104 22-23 0.32587739199323096 1104 24-25 0.324657466633937 1104 26-27 0.33675904762947795 1104 28-29 0.30621296719352387 1104 30-31 0.31435239656281766 1104 32-33 0.35715203238262205 1104 34-35 0.39829772134761043 1104 36-37 0.3683824310979773 1104 38-39 0.3870022006862541 1104 40-41 0.36824101075790594 1104 42-43 0.4039148575358027 1104 44-45 0.4377030220099769 1104 46-47 0.439081607494046 1104 48-49 0.36493357228464873 1104 50-51 0.3750862365445258 1104 52-53 0.41742886214764496 1104 54-55 0.43540949289855035 1104 56-57 0.4547852044243683 1104 58-59 0.41819740710351283 1104 60-61 0.4349724030189037 1104 62-63 0.4166523984162467 1104 64-65 0.3967025859049329 1104 66-67 0.39452080165435177 1104 68-69 0.35326122566367246 1104 70-71 0.35681366402037895 1104 72-73 0.3640557541670404 1104 74-75 0.3608870441228902 1104 76-77 0.23928176432092485 1104 78-79 0.36681647104156667 1104 80-81 0.3979053726160231 1104 82-83 0.420617697070341 1104 84-85 0.3959493292069709 1104 86-87 0.3800039790051528 1104 88-89 0.3372363102226359 1104 90-91 0.33507015415737484 1104 92-93 0.3495670693271933 1104 94-95 0.3283565715499215 1104 96-97 0.31996413100971566 1104 98-99 0.30537323636538005 1104 100 0.33541485437938334 1105 1 1.3799890757768587 1105 2 1.014594775965758 1105 3 1.3611173249790234 1105 4 0.8371889280744682 1105 5 0.43521822409433497 1105 6 0.9793592424379227 1105 7 0.6910231842419208 1105 8 0.3549843833667836 1105 9 0.32835463568200396 1105 10-11 0.25441862476748867 1105 12-13 0.22756053469692716 1105 14-15 0.278449152862116 1105 16-17 0.3021608579879853 1105 18-19 0.2937274019592948 1105 20-21 0.2911189670004788 1105 22-23 0.2831214242610258 1105 24-25 0.29518613540714256 1105 26-27 0.30337624541047603 1105 28-29 0.30325326949890297 1105 30-31 0.31200899268065996 1105 32-33 0.3240913146576716 1105 34-35 0.3345759534300399 1105 36-37 0.3039214331165283 1105 38-39 0.34368693172303466 1105 40-41 0.34898629118573865 1105 42-43 0.3178441528898972 1105 44-45 0.26968561288110493 1105 46-47 0.316466449126807 1105 48-49 0.2870546511910632 1105 50-51 0.2917012076865788 1105 52-53 0.28142950135709555 1105 54-55 0.3541309090071607 1105 56-57 0.35057736778972526 1105 58-59 0.33610663439370825 1105 60-61 0.31247541355193675 1105 62-63 0.2716559959616234 1105 64-65 0.24796572535989014 1105 66-67 0.25414297571553846 1105 68-69 0.2398765075976499 1105 70-71 0.22942364720996267 1105 72-73 0.2196388239483511 1105 74-75 0.2744016783674468 1105 76-77 0.17701102902708854 1105 78-79 0.27651274530273184 1105 80-81 0.2990536151580372 1105 82-83 0.32322350214774076 1105 84-85 0.30999803334764664 1105 86-87 0.2999912065354451 1105 88-89 0.29593054305434663 1105 90-91 0.28721416838236635 1105 92-93 0.2672873182551143 1105 94-95 0.2647895831644007 1105 96-97 0.22302664551509466 1105 98-99 0.2796088159836394 1105 100 0.25181427515325083 1106 1 1.37090742052602 1106 2 1.0246308973319849 1106 3 1.3511251672006388 1106 4 0.8100895010737901 1106 5 0.42710641160395824 1106 6 0.9551994382622837 1106 7 0.6901102058888284 1106 8 0.3318171288087086 1106 9 0.3230373935877182 1106 10-11 0.23117728334063514 1106 12-13 0.21998908660500405 1106 14-15 0.26277951816252454 1106 16-17 0.24335290768243567 1106 18-19 0.2052496115225395 1106 20-21 0.2420584855402126 1106 22-23 0.25689267878671274 1106 24-25 0.2652578279315776 1106 26-27 0.23458238436491996 1106 28-29 0.25727682444121314 1106 30-31 0.2404536311944554 1106 32-33 0.30503592000203383 1106 34-35 0.314497804085228 1106 36-37 0.2758505805954883 1106 38-39 0.275464515528725 1106 40-41 0.3008378795390101 1106 42-43 0.30015951451952105 1106 44-45 0.28054628583851837 1106 46-47 0.2961911157514834 1106 48-49 0.26146716221661137 1106 50-51 0.273264731836953 1106 52-53 0.23356769746467876 1106 54-55 0.25727126425125135 1106 56-57 0.2666726260793695 1106 58-59 0.2283233422892792 1106 60-61 0.2215981215271512 1106 62-63 0.19528658844864566 1106 64-65 0.1662332199267027 1106 66-67 0.1609744442062322 1106 68-69 0.1677321494255537 1106 70-71 0.15908782875347782 1106 72-73 0.08597795348549653 1106 74-75 0.1355529011681753 1106 76-77 0.026848852064457418 1106 78-79 0.15921272908297368 1106 80-81 0.19480477401792484 1106 82-83 0.20742067731833913 1106 84-85 0.22233126809448578 1106 86-87 0.22054345480808735 1106 88-89 0.19357004058183946 1106 90-91 0.21056967675664495 1106 92-93 0.19263803916975775 1106 94-95 0.17729502926216156 1106 96-97 0.2128869639033404 1106 98-99 0.22831995227035762 1106 100 0.21046440749651651 1107 1 1.310236019654436 1107 2 0.9397953902665144 1107 3 1.2974672895225403 1107 4 0.7945155323372646 1107 5 0.3955047211296687 1107 6 0.9470417334007095 1107 7 0.6672493650293418 1107 8 0.30741112517196 1107 9 0.26139112717574875 1107 10-11 0.19562970966047288 1107 12-13 0.1151487946139369 1107 14-15 0.1694652488713615 1107 16-17 0.1892915463306366 1107 18-19 0.1489749540490024 1107 20-21 0.13538202640243213 1107 22-23 0.19446024811577445 1107 24-25 0.17517057324176477 1107 26-27 0.14264348190308596 1107 28-29 0.17871432936448883 1107 30-31 0.15808949131538697 1107 32-33 0.1891678364666589 1107 34-35 0.20449714279435938 1107 36-37 0.17244657454823908 1107 38-39 0.1623752340935667 1107 40-41 0.16886164431394945 1107 42-43 0.1706218367766681 1107 44-45 0.1541167474100078 1107 46-47 0.21098045593149806 1107 48-49 0.17854931465925716 1107 50-51 0.1362328628317684 1107 52-53 0.17060969977305263 1107 54-55 0.196448506832688 1107 56-57 0.215750500697645 1107 58-59 0.17281794565399622 1107 60-61 0.16948203538580486 1107 62-63 0.14147021177664243 1107 64-65 0.14263543561401093 1107 66-67 0.155133061409515 1107 68-69 0.1596858406271977 1107 70-71 0.1787750185199286 1107 72-73 0.16691343593736718 1107 74-75 0.1430948626654498 1107 76-77 -0.01227211850649823 1107 78-79 0.14479771783879158 1107 80-81 0.18917822083169256 1107 82-83 0.18629719674736123 1107 84-85 0.21126330705644847 1107 86-87 0.159033885775564 1107 88-89 0.18309983694072685 1107 90-91 0.19975973401000147 1107 92-93 0.2069419814061888 1107 94-95 0.18086919553185155 1107 96-97 0.1803616424117429 1107 98-99 0.1907160072909626 1107 100 0.18131196803765093 1108 1 1.2669947936512678 1108 2 0.886015970629284 1108 3 1.2361721882378127 1108 4 0.7614802644224596 1108 5 0.34010286893425246 1108 6 0.8692853310154618 1108 7 0.6104305321184782 1108 8 0.24772921865249486 1108 9 0.1952965803645199 1108 10-11 0.10673467530073566 1108 12-13 0.10794003688151577 1108 14-15 0.118645214569014 1108 16-17 0.12282644520302455 1108 18-19 0.09820023186198057 1108 20-21 0.10487866319970607 1108 22-23 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-0.9215358616687404 2316 96-97 -0.9785844531231511 2316 98-99 -1.1015341388084465 2316 100 -1.0640037137667804 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 37.0 7 759.0 8 937.0 9 388.0 10 406.0 11 685.0 12 970.0 13 1589.0 14 3764.0 15 9903.0 16 22108.0 17 34553.0 18 42886.0 19 52976.0 20 69910.0 21 99183.0 22 142930.0 23 234123.0 24 386409.0 25 484926.0 26 483437.0 27 410455.0 28 362386.0 29 374568.0 30 429225.0 31 510636.0 32 624249.0 33 794852.0 34 1085044.0 35 1666489.0 36 2977821.0 37 5834085.0 38 7555414.0 39 2083220.0 40 4184.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 16.968815879093622 32.47031490499365 28.223905722329352 22.336963493583376 2 21.362943560417417 34.51761099401508 25.8436654771545 18.275779968413005 3 26.27849570256969 25.294990828177806 27.064784690082462 21.361728779170043 4 19.467831335129468 27.731224266440123 24.51913091898711 28.2818134794433 5 21.581953217590343 32.829387696587276 26.052835685881483 19.535823399940902 6 29.50266005408596 27.337492892023345 24.834185792708812 18.325661261181878 7 21.045272945759198 25.61593014488246 32.715829765674194 20.622967143684146 8 27.607757357456915 26.787318170601083 25.233811989437534 20.37111248250447 9 26.691913156316705 25.218866501905918 27.137893157590526 20.95132718418685 10-11 20.592231916340218 30.064012464361994 28.982824336604825 20.360931282692963 12-13 20.416507329878055 26.68580811257371 29.021044850859084 23.876639706689144 14-15 20.325794094545234 33.38835251466399 25.787066490845216 20.498786899945557 16-17 23.81878681644247 26.436848680992586 25.89507797716612 23.849286525398824 18-19 20.350318550998495 29.836890897753026 26.017709875717493 23.79508067553099 20-21 23.69186305894303 29.997481465851088 25.971664736138933 20.338990739066944 22-23 23.706132644263818 26.684349248303924 29.24665228301006 20.362865824422197 24-25 20.380387410272167 29.89551780240983 25.78460116101037 23.939493626307623 26-27 20.446404015359825 26.49065374389268 32.66848421954919 20.394458021198304 28-29 20.418627308865915 26.651768383911232 32.59366489448538 20.33593941273747 30-31 20.392881851859947 26.534656289563756 32.655737329111226 20.416724529465075 32-33 20.513773816614297 26.721946912548155 32.361511778510696 20.402767492326845 34-35 20.543771277600676 26.78970244402577 32.253510267387426 20.413016010986134 36-37 20.413642973546942 26.773037617269402 32.41989360801706 20.393425801166593 38-39 20.471243220829756 26.7079566370559 32.48055644424724 20.340243697867095 40-41 20.58759441872913 26.740805502131764 32.36114001084037 20.310460068298735 42-43 20.50871652233866 26.841551312768136 32.2694380342263 20.38029413066691 44-45 20.5578846816313 26.891363117996253 32.22802816930865 20.3227240310638 46-47 20.580495104847365 26.988547571272235 32.12397537834447 20.306981945535934 48-49 20.657173164872923 26.993917730935525 32.01010902907636 20.338800075115195 50-51 20.625032306506807 27.139848556059743 31.91638426173395 20.3187348756995 52-53 20.799729478147896 27.210775358752638 31.561806077043887 20.42768908605558 54-55 21.136707580829707 27.449080283288524 30.797301934012317 20.61691020186945 56-57 21.54939848053737 28.030005228980464 29.596318824639607 20.82427746584256 58-59 21.66335620977321 28.62735591755162 28.831827016169814 20.877460856505365 60-61 21.74645944365896 28.9356256322297 28.342095889267476 20.975819034843862 62-63 21.783876968444524 29.178017273965818 27.994478927187828 21.04362683040183 64-65 21.749207062182332 29.255521973058695 28.00266733528708 20.992603629471894 66-67 21.677453795181627 29.1860325569945 28.12269600609245 21.013817641731425 68-69 21.56493193727251 29.118044807643212 28.276491908802466 21.040531346281817 70-71 21.46425554981162 29.02352598971595 28.390724833600956 21.121493626871473 72-73 21.431834078538348 28.927895729266844 28.3916992943894 21.248570897805404 74-75 21.538129848916707 28.941745656615137 28.2354360032415 21.284688491226657 76-77 21.63227245190144 29.02840409616976 28.01406107683358 21.32526237509522 78-79 21.717980443693254 29.188485586775116 27.665423859184312 21.42811011034732 80-81 21.825098751131723 29.36721824562738 27.268406396847478 21.539276606393422 82-83 21.77855798495338 29.466624554055347 26.971723132041305 21.78309432894997 84-85 21.748530948728746 29.43585297327252 26.852671820920605 21.96294425707813 86-87 21.74630903678523 29.38560303437287 26.78656321459797 22.081524714243926 88-89 21.788762931097846 29.312631535160254 26.68131286255494 22.217292671186957 90-91 21.811771070482287 29.236986405966935 26.618079334786536 22.333163188764242 92-93 21.851437132541125 29.183754173536364 26.616855478585954 22.347953215336556 94-95 21.892753811454536 29.09976204695992 26.565738980266957 22.441745161318586 96-97 21.959604666857242 28.966482791351066 26.5215704315237 22.55234211026799 98-99 21.944334263423215 28.913565287504483 26.50233246462402 22.63976798444828 100 21.89415827195661 28.848009528804404 26.453629348938584 22.804202850300406 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 19667.0 1 15276.5 2 9131.5 3 7011.0 4 6424.5 5 6332.0 6 6605.5 7 6766.5 8 6831.5 9 7057.0 10 7819.0 11 15310.0 12 112898.5 13 133906.5 14 63427.5 15 67716.5 16 77592.0 17 86409.5 18 94147.0 19 102524.0 20 113428.0 21 130261.0 22 154460.0 23 180545.0 24 205571.0 25 235686.0 26 269983.5 27 309651.5 28 360469.5 29 410996.5 30 451070.5 31 489123.5 32 531105.0 33 566863.5 34 599156.0 35 640057.5 36 680549.0 37 714971.5 38 758174.0 39 789203.5 40 803822.5 41 834799.0 42 874459.0 43 883167.0 44 869591.0 45 873466.5 46 884981.0 47 888300.5 48 884347.5 49 877177.5 50 872781.5 51 870146.0 52 857755.5 53 817731.5 54 761144.0 55 708443.0 56 654277.0 57 591079.0 58 522192.0 59 445581.0 60 364047.0 61 294049.5 62 231543.0 63 174160.0 64 129512.0 65 95220.5 66 69699.5 67 53060.0 68 40681.5 69 30544.5 70 22678.0 71 17503.5 72 14117.5 73 10854.5 74 8495.0 75 7000.5 76 5112.0 77 2998.5 78 1713.5 79 1078.0 80 657.5 81 414.0 82 270.0 83 174.0 84 106.0 85 71.5 86 43.5 87 30.5 88 27.5 89 20.0 90 13.5 91 8.0 92 3.5 93 4.0 94 4.5 95 1.5 96 0.0 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.32294329915054437 2 0.006339249057335372 3 2.4084591715960424 4 7.541391693649854E-4 5 4.480034669494962E-5 6 0.008276864051891942 7 0.007735526529327969 8 3.621361357841761E-4 9 3.7333622245791354E-6 10-11 7.653392560387227E-5 12-13 0.0 14-15 0.0 16-17 2.0533492235185244E-5 18-19 0.0 20-21 3.173357890892265E-4 22-23 5.226707114410789E-5 24-25 4.8720377030757713E-4 26-27 0.0035784276922591015 28-29 0.0061955146116890744 30-31 0.005982712964888065 32-33 0.015493453232003412 34-35 0.009882209808460971 36-37 0.005025105554283516 38-39 0.005376041603393955 40-41 0.00854193276983706 42-43 0.01915401489320325 44-45 0.03408933047263208 46-47 0.03328665759434757 48-49 0.039689373809500786 50-51 0.04000297623636544 52-53 0.061619143516678625 54-55 0.0332997243621336 56-57 0.022056704022813532 58-59 0.016652662202735233 60-61 0.017895871823520083 62-63 0.024839925561237274 64-65 0.04468834582821225 66-67 0.04742863370105333 68-69 0.05044519037851327 70-71 0.06047113463262054 72-73 0.06818799435082562 74-75 0.05900018991613637 76-77 0.05922792501183569 78-79 0.05375668267171497 80-81 0.05147746503360941 82-83 0.04795877113694357 84-85 0.0499579866082057 86-87 0.05760017908191918 88-89 0.06435009798395826 90-91 0.06016313224909277 92-93 0.06455169954408553 94-95 0.07284349704487579 96-97 0.07194749011097681 98-99 0.047213965373140036 100 0.0069963208088612994 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2.6785507E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 35.82795047798244 #Duplication Level Percentage of deduplicated Percentage of total 1 63.8579461220051 22.878993312848696 2 18.09622398056316 12.967012332281902 3 6.9844083670247485 7.507111112753065 4 3.2637259219941317 4.677304428276533 5 1.8141326575710073 3.2498327507972364 6 1.1733529961193054 2.5223299822892877 7 0.815298469575953 2.0447331234919477 8 0.5901201590645981 1.691423666802043 9 0.4717498411718285 1.5211646952750317 >10 2.519819655049521 16.72140929415948 >50 0.22528772214493129 5.73074597212038 >100 0.18445487021790444 11.25056105874993 >500 0.002628521139300143 0.5619582758151658 >1k 7.353854805763778E-4 0.4891567850539001 >5k 6.290775150626771E-5 0.15326219478152356 >10k+ 5.242312625165067E-5 6.033001014503898 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 1495188 5.582078397844028 No Hit AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 34022 0.12701644960463135 No Hit TAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 32305 0.12060626666502897 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.09319592121216895 1.1200086673737406E-5 0.0 0.0 7.466724449158271E-5 2 0.10539654896209356 1.4933448898316541E-5 0.0 0.0 8.586733116532011E-5 3 0.11922118927971011 3.360026002121222E-5 0.0 0.0 9.333405561447838E-5 4 0.1221929456104751 3.7333622245791355E-5 0.0 0.0 1.0080078006363665E-4 5 0.12257374855738216 5.226707114410789E-5 0.0 0.0 1.0080078006363665E-4 6 0.12268574942411954 5.226707114410789E-5 0.0 0.0 1.0453414228821578E-4 7 0.12289108434647139 5.9733795593266166E-5 0.0 0.0 1.2320095341111147E-4 8 0.12338015479789126 8.586733116532011E-5 3.7333622245791354E-6 0.0 1.2693431563569059E-4 9 0.12363402342916265 8.960069338989925E-5 3.7333622245791354E-6 0.0 1.2693431563569059E-4 10-11 0.12376095774479834 1.1946759118653233E-4 3.7333622245791354E-6 0.0 1.3626772119713844E-4 12-13 0.1238393583515145 1.2693431563569059E-4 3.7333622245791354E-6 0.0 1.5306785120774454E-4 14-15 0.12394202581269043 1.4746780787087586E-4 3.7333622245791354E-6 0.0 1.7360134344292978E-4 16-17 0.12406709344721381 1.6426793788148195E-4 5.600043336868703E-6 0.0 1.792013867797985E-4 18-19 0.12424816151510591 1.7733470566750893E-4 1.4933448898316541E-5 0.0 1.9413483567811503E-4 20-21 0.12449829678415272 1.9973487901498374E-4 1.4933448898316541E-5 0.0 2.0533492235185244E-4 22-23 0.12489963322329498 2.07201603464142E-4 1.4933448898316541E-5 0.0 2.2213505236245854E-4 24-25 0.1259337745595034 2.258684145870377E-4 1.4933448898316541E-5 0.0 2.3520182014848552E-4 26-27 0.129263933663828 2.426685445976438E-4 1.4933448898316541E-5 0.0 2.4640190682222293E-4 28-29 0.13819973614835812 2.594686746082499E-4 1.4933448898316541E-5 0.0 2.6320203683282903E-4 30-31 0.15761508639728194 2.7440212350656644E-4 1.4933448898316541E-5 0.0 2.7813548573114556E-4 32-33 0.19218415391577243 2.912022535171726E-4 1.4933448898316541E-5 1.8666811122895677E-6 3.0053565907862034E-4 34-35 0.2510536761540485 3.061357024154891E-4 1.4933448898316541E-5 5.600043336868703E-6 3.173357890892265E-4 36-37 0.34053490195276126 3.117357457523578E-4 1.4933448898316541E-5 7.466724449158271E-6 3.416026435489909E-4 38-39 0.4661121404198173 3.1546910797693693E-4 1.4933448898316541E-5 7.466724449158271E-6 3.658694980087552E-4 40-41 0.6255864412049397 3.3600260021212215E-4 1.4933448898316541E-5 7.466724449158271E-6 3.826696280193613E-4 42-43 0.8213210225962868 3.5466941133501786E-4 1.4933448898316541E-5 7.466724449158271E-6 3.9946975802996747E-4 44-45 1.056926045865027 3.6773617912104484E-4 1.4933448898316541E-5 9.333405561447839E-6 4.0693648247912577E-4 46-47 1.3468664229502916 3.789362657947822E-4 1.4933448898316541E-5 1.1200086673737406E-5 4.14403206928284E-4 48-49 1.6944573795075075 3.826696280193614E-4 1.4933448898316541E-5 1.1200086673737406E-5 4.218699313774423E-4 50-51 2.087132418288741 3.976030769176779E-4 1.4933448898316541E-5 1.1200086673737406E-5 4.386700613880484E-4 52-53 2.5087802146138207 4.27469974714311E-4 1.4933448898316541E-5 1.1200086673737406E-5 4.5360351028636493E-4 54-55 2.97239100234317 4.498701480617858E-4 1.8666811122895677E-5 1.1200086673737406E-5 4.7040364029697103E-4 56-57 3.495907320328116 4.7227032140926065E-4 1.8666811122895677E-5 1.1200086673737406E-5 5.002705380936041E-4 58-59 4.081152916015366 4.83470408082998E-4 1.8666811122895677E-5 1.1200086673737406E-5 5.226707114410789E-4 60-61 4.715346623829072 4.96537175869025E-4 1.8666811122895677E-5 1.1200086673737406E-5 5.469375659008433E-4 62-63 5.376248804997419 5.002705380936041E-4 1.8666811122895677E-5 1.1200086673737406E-5 5.581376525745807E-4 64-65 6.061443974161102 5.170706681042103E-4 1.8666811122895677E-5 1.1200086673737406E-5 5.712044203606077E-4 66-67 6.786431184595461 5.301374358902373E-4 1.8666811122895677E-5 1.1200086673737406E-5 5.78671144809766E-4 68-69 7.557420884361084 5.413375225639746E-4 1.8666811122895677E-5 1.1200086673737406E-5 5.917379125957929E-4 70-71 8.361908176686743 5.581376525745807E-4 1.8666811122895677E-5 1.1200086673737406E-5 5.973379559326617E-4 72-73 9.187707367271413 5.861378692589242E-4 1.8666811122895677E-5 1.1200086673737406E-5 6.066713614941094E-4 74-75 10.032552305244774 6.178714481678469E-4 1.8666811122895677E-5 1.4933448898316541E-5 6.160047670555573E-4 76-77 10.896965661318264 6.365382592907426E-4 1.8666811122895677E-5 1.680013001060611E-5 6.365382592907426E-4 78-79 11.78470879793315 6.533383893013487E-4 1.8666811122895677E-5 1.8666811122895677E-5 6.533383893013487E-4 80-81 12.695496112879251 6.701385193119548E-4 1.8666811122895677E-5 1.8666811122895677E-5 6.626717948627966E-4 82-83 13.621144076160292 6.888053304348505E-4 1.8666811122895677E-5 1.8666811122895677E-5 6.738718815365339E-4 84-85 14.555854776241496 7.03738779333167E-4 1.8666811122895677E-5 2.6133535572053945E-5 6.9067201154714E-4 86-87 15.499676746831785 7.224055904560627E-4 1.8666811122895677E-5 2.6133535572053945E-5 7.000054171085879E-4 88 16.214854548021062 7.354723582420897E-4 1.8666811122895677E-5 2.6133535572053945E-5 7.056054604454566E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AAGCAGT 194680 0.0 84.14661 1 GGTATCA 202115 0.0 83.76575 8 AGTGGTA 201275 0.0 83.5635 5 GTGGTAT 201645 0.0 83.375244 6 GCAGTGG 202365 0.0 83.19303 3 TGGTATC 203675 0.0 83.046646 7 GTATCAA 206080 0.0 82.02411 9 AGCAGTG 207575 0.0 80.784515 2 CAGTGGT 209280 0.0 80.22018 4 CAACGCA 200155 0.0 43.39624 12-13 GAGTACT 193945 0.0 43.106308 20-21 ACGCAGA 202055 0.0 43.09278 14-15 ATCAACG 202160 0.0 43.077286 10-11 AGAGTAC 202320 0.0 42.919624 18-19 AGTACTT 202005 0.0 42.608604 20-21 TCAACGC 199555 0.0 42.256622 12-13 AACGCAG 201305 0.0 41.974457 14-15 CGCAGAG 203515 0.0 41.513115 16-17 TATCAAC 205310 0.0 41.116714 10-11 GTACTTT 203805 0.0 41.019337 22-23 >>END_MODULE