FastQCFastQC Report
Thu 26 May 2016
SRR1660733_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660733_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35828346
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT13405013.7414537640113226No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC555910.1551592697022631Illumina Single End PCR Primer 1 (100% over 50bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA502860.1403525577206383No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1730700.085.166398
TGGTATC1755200.083.707257
AGTGGTA1770450.083.457015
GTGGTAT1776000.083.11686
GTATCAA1776100.082.926099
GCAGTGG1862300.079.7941743
AAGCAGT1875150.079.22661
CAGTGGT1901900.077.630124
AGCAGTG1999700.074.421232
CAACGCA1682200.044.83735712-13
ATCAACG1700400.044.42103610-11
ACGCAGA1718300.043.9615314-15
TCAACGC1679100.043.92490412-13
AACGCAG1700000.043.5387614-15
AGAGTAC1730200.043.09324618-19
CGCAGAG1735850.042.56693616-17
GAGTACT1704300.042.4924920-21
TATCAAC1757950.041.8144810-11
AGTACTT1815500.040.7713920-21
GTACTTT1825550.039.65575822-23
Produced by FastQC (version 0.11.5)