##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660733_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 35828346 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.84044677920661 34.0 31.0 34.0 30.0 34.0 2 31.961535902327167 34.0 31.0 34.0 30.0 34.0 3 32.02367910034139 34.0 31.0 34.0 30.0 34.0 4 35.43084470045031 37.0 35.0 37.0 33.0 37.0 5 35.40444900805636 37.0 35.0 37.0 33.0 37.0 6 35.479549125711806 37.0 35.0 37.0 33.0 37.0 7 35.45878327735252 37.0 35.0 37.0 33.0 37.0 8 35.449593068013804 37.0 35.0 37.0 33.0 37.0 9 37.131416727972876 39.0 37.0 39.0 33.0 39.0 10-11 37.090636349777355 39.0 37.0 39.0 33.0 39.0 12-13 37.08088023376798 39.0 37.0 39.0 33.0 39.0 14-15 38.44395105484356 40.0 38.0 41.0 34.0 41.0 16-17 38.42624345539144 40.0 38.0 41.0 34.0 41.0 18-19 38.392152850148314 40.0 38.0 41.0 33.5 41.0 20-21 38.2907964548517 40.0 38.0 41.0 33.5 41.0 22-23 38.155251082480895 40.0 38.0 41.0 33.0 41.0 24-25 38.11665517576502 40.0 38.0 41.0 33.0 41.0 26-27 38.07075575020963 40.0 38.0 41.0 33.0 41.0 28-29 37.97236353026177 40.0 38.0 41.0 33.0 41.0 30-31 37.87190159434097 40.0 38.0 41.0 32.5 41.0 32-33 37.7474117281328 40.0 38.0 41.0 32.0 41.0 34-35 37.599882129641145 40.0 37.5 41.0 31.5 41.0 36-37 37.435733008718856 40.0 37.0 41.0 31.0 41.0 38-39 37.110187015052276 40.0 36.0 41.0 31.0 41.0 40-41 37.05715048916855 40.0 36.0 41.0 30.5 41.0 42-43 37.077252072981544 40.0 36.0 41.0 30.5 41.0 44-45 37.08708518110214 40.0 36.0 41.0 31.0 41.0 46-47 37.1833557150531 40.0 36.0 41.0 31.0 41.0 48-49 37.09505223880555 40.0 36.0 41.0 31.0 41.0 50-51 35.75496401648013 38.5 34.5 40.0 29.0 40.5 52-53 35.95545642827051 38.5 35.0 39.5 29.5 40.5 54-55 36.502221983677394 39.5 35.0 41.0 28.5 41.0 56-57 36.264332757643906 40.0 35.0 41.0 28.0 41.0 58-59 36.11009556511484 39.0 35.0 41.0 28.0 41.0 60-61 35.89293332156612 39.0 35.0 41.0 27.0 41.0 62-63 35.673161942781284 39.0 35.0 41.0 26.5 41.0 64-65 35.412781404980294 38.5 35.0 41.0 26.0 41.0 66-67 35.119196292231855 37.5 35.0 40.0 26.0 41.0 68-69 34.82553889035235 37.0 34.0 40.0 26.0 41.0 70-71 34.51338764005461 36.5 34.0 39.5 26.0 41.0 72-73 34.164863066801914 36.0 34.0 39.0 25.5 41.0 74-75 33.78590109909065 35.5 34.0 39.0 25.0 40.5 76-77 33.39210870912099 35.0 33.0 37.5 24.5 40.0 78-79 33.021348682967385 35.0 33.0 37.0 24.0 39.0 80-81 32.69916166378431 35.0 33.0 36.5 23.5 39.0 82-83 32.38502945405294 35.0 33.0 36.0 23.0 38.0 84-85 32.09570265956458 35.0 33.0 36.0 22.5 37.0 86-87 31.87389590912179 35.0 32.5 35.0 21.5 37.0 88-89 31.662718689274687 35.0 32.0 35.0 20.0 36.5 90-91 31.48639873021211 35.0 32.0 35.0 20.0 36.0 92-93 31.33147011586859 35.0 32.0 35.0 20.0 36.0 94-95 31.148167445407612 35.0 32.0 35.0 19.5 36.0 96-97 31.0134851606044 35.0 31.5 35.0 19.0 35.0 98-99 30.91328073029104 35.0 31.0 35.0 19.0 35.0 100 30.834834853944976 35.0 31.0 35.0 18.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5109887181303598 1101 2 0.5314974728579323 1101 3 0.5291065198758389 1101 4 0.4406480822578942 1101 5 0.48324762256530107 1101 6 0.4362321904710953 1101 7 0.4460562298259845 1101 8 0.4694233097626537 1101 9 0.5347741868644817 1101 10-11 0.5621881639281128 1101 12-13 0.5472028321555413 1101 14-15 0.6722602422197141 1101 16-17 0.6385224687679099 1101 18-19 0.6182575226103566 1101 20-21 0.6826760100706508 1101 22-23 0.6994140153650079 1101 24-25 0.7128539201205015 1101 26-27 0.6522924155773211 1101 28-29 0.6629361163805925 1101 30-31 0.6320577542538715 1101 32-33 0.6188521062080667 1101 34-35 0.6393463825500518 1101 36-37 0.6033935729802877 1101 38-39 0.6145438789397204 1101 40-41 0.6411636441961761 1101 42-43 0.6214790646118615 1101 44-45 0.6551521883854434 1101 46-47 0.6692260368425806 1101 48-49 0.7182632294800726 1101 50-51 0.6203286357715214 1101 52-53 0.6571466678332811 1101 54-55 0.6945287341317226 1101 56-57 0.7394484680467457 1101 58-59 0.6521527709873567 1101 60-61 0.6588598642120544 1101 62-63 0.6592900845829988 1101 64-65 0.5996840369670124 1101 66-67 0.6042642734765664 1101 68-69 0.6099639490983435 1101 70-71 0.5626592550243714 1101 72-73 0.52384258602941 1101 74-75 0.5170834792659704 1101 76-77 0.5054273933511979 1101 78-79 0.5016404959486209 1101 80-81 0.4444900073160909 1101 82-83 0.44398653071365857 1101 84-85 0.4693316076579137 1101 86-87 0.3852010689113108 1101 88-89 0.3736019045710286 1101 90-91 0.365325153341594 1101 92-93 0.34903499789891157 1101 94-95 0.4026889867829553 1101 96-97 0.3734594804984752 1101 98-99 0.3153640096883805 1101 100 0.3061949200562104 1102 1 0.4851643608474383 1102 2 0.5082627374537303 1102 3 0.5184991903132499 1102 4 0.433872098175172 1102 5 0.44215014127167507 1102 6 0.4031791358279122 1102 7 0.404405164083542 1102 8 0.4301401401954621 1102 9 0.5124885169026498 1102 10-11 0.499741498526582 1102 12-13 0.5014870942249843 1102 14-15 0.6107593206900077 1102 16-17 0.5804075733060898 1102 18-19 0.5617647418510003 1102 20-21 0.592714467043713 1102 22-23 0.5974335248316436 1102 24-25 0.5755061968475772 1102 26-27 0.575606199847158 1102 28-29 0.6153359037058479 1102 30-31 0.6037070219781739 1102 32-33 0.5776984433144818 1102 34-35 0.5826729468803791 1102 36-37 0.5974231922134692 1102 38-39 0.5970240143462817 1102 40-41 0.5569034969159574 1102 42-43 0.5846903251699089 1102 44-45 0.6001633877966484 1102 46-47 0.6278705743664474 1102 48-49 0.649688891123219 1102 50-51 0.5888414696497151 1102 52-53 0.6300330645488046 1102 54-55 0.6597372162518766 1102 56-57 0.6955021645901809 1102 58-59 0.5778773401456476 1102 60-61 0.6001860730908533 1102 62-63 0.5935104705448921 1102 64-65 0.5546845314654902 1102 66-67 0.5524888108611563 1102 68-69 0.5423588532801134 1102 70-71 0.5259398970541582 1102 72-73 0.5053179482747296 1102 74-75 0.5061810263723814 1102 76-77 0.4900437422233139 1102 78-79 0.47717278145243114 1102 80-81 0.47165946362096633 1102 82-83 0.4540257253120501 1102 84-85 0.44448784899726945 1102 86-87 0.4105606490385476 1102 88-89 0.37163742868591854 1102 90-91 0.3470377769940356 1102 92-93 0.33986082667886564 1102 94-95 0.39296644050350693 1102 96-97 0.3426561968551525 1102 98-99 0.33013538996756253 1102 100 0.30903164917062753 1103 1 0.4074536580978254 1103 2 0.4546419238352577 1103 3 0.4633098365747301 1103 4 0.3906189754677598 1103 5 0.38343716654124194 1103 6 0.40790938264128584 1103 7 0.3910609254577295 1103 8 0.4322282290095387 1103 9 0.5047887886701119 1103 10-11 0.4718921626472792 1103 12-13 0.49813995006329037 1103 14-15 0.5805784790986763 1103 16-17 0.5712511492027303 1103 18-19 0.5634018394217222 1103 20-21 0.5742000136598691 1103 22-23 0.5789981763162757 1103 24-25 0.6429206306030935 1103 26-27 0.5905981308965664 1103 28-29 0.59260012980684 1103 30-31 0.558624821252053 1103 32-33 0.5218427813593038 1103 34-35 0.5417510468047269 1103 36-37 0.5562981751590357 1103 38-39 0.5575579746721147 1103 40-41 0.5288568494986308 1103 42-43 0.5653453586513848 1103 44-45 0.5717974579692537 1103 46-47 0.5876580229496611 1103 48-49 0.6401698564968399 1103 50-51 0.5773919515315953 1103 52-53 0.5802206935174965 1103 54-55 0.6492903057100605 1103 56-57 0.6896383236044414 1103 58-59 0.6336974594603362 1103 60-61 0.6295252022800071 1103 62-63 0.5741231858213993 1103 64-65 0.5631402477439238 1103 66-67 0.5591681188827309 1103 68-69 0.5751939365728305 1103 70-71 0.5366818583350081 1103 72-73 0.517311687445023 1103 74-75 0.5321404036131625 1103 76-77 0.5412331380964233 1103 78-79 0.5203486615004849 1103 80-81 0.4627134477260313 1103 82-83 0.47322749911933215 1103 84-85 0.4433472543263548 1103 86-87 0.4175275099974378 1103 88-89 0.44232995796993535 1103 90-91 0.42341877062120403 1103 92-93 0.38264977731353156 1103 94-95 0.45741358556431777 1103 96-97 0.4018753543004294 1103 98-99 0.39072459011805094 1103 100 0.38119901594529537 1104 1 0.34537733317876373 1104 2 0.39632527366138603 1104 3 0.36535544804925735 1104 4 0.29915543145232704 1104 5 0.2972900907354017 1104 6 0.2635718775902234 1104 7 0.2794070119866987 1104 8 0.30154751423881265 1104 9 0.3399334763166948 1104 10-11 0.3126779577361276 1104 12-13 0.3369241752212133 1104 14-15 0.41542280792201325 1104 16-17 0.4197268289617 1104 18-19 0.38495074113782124 1104 20-21 0.31117725469208324 1104 22-23 0.36232188862680204 1104 24-25 0.40188523748379623 1104 26-27 0.35108849609959236 1104 28-29 0.36111008818140533 1104 30-31 0.31364114167907786 1104 32-33 0.311512020573268 1104 34-35 0.2836210392939904 1104 36-37 0.2781990031673587 1104 38-39 0.28433080919604237 1104 40-41 0.3068481804166723 1104 42-43 0.26587614414020777 1104 44-45 0.27334398441173136 1104 46-47 0.3130973464826283 1104 48-49 0.31993611024001467 1104 50-51 0.29807382000092986 1104 52-53 0.30266457141141956 1104 54-55 0.4164585260839928 1104 56-57 0.43719342873765754 1104 58-59 0.3764633775958899 1104 60-61 0.42245255194409026 1104 62-63 0.3782919787408261 1104 64-65 0.35046549457631926 1104 66-67 0.3591419051975677 1104 68-69 0.33607979630822626 1104 70-71 0.33623877563092464 1104 72-73 0.30829082398599184 1104 74-75 0.3150107976749368 1104 76-77 0.3313876864651135 1104 78-79 0.3095584529857902 1104 80-81 0.25610148560875245 1104 82-83 0.2616371688437553 1104 84-85 0.22183486851464096 1104 86-87 0.22242470232277967 1104 88-89 0.22045587132002709 1104 90-91 0.21388420838552236 1104 92-93 0.22502515658118227 1104 94-95 0.28348940865581795 1104 96-97 0.24663804151329316 1104 98-99 0.21245090765554764 1104 100 0.17973834657581378 1105 1 0.2701920177868722 1105 2 0.29837636189572336 1105 3 0.29665123649254355 1105 4 0.2570919784680612 1105 5 0.26247130000858476 1105 6 0.24461222695289564 1105 7 0.27573934764738084 1105 8 0.28897351100440716 1105 9 0.2745206988049773 1105 10-11 0.28673141982765316 1105 12-13 0.29928909190279285 1105 14-15 0.36054547812078397 1105 16-17 0.31662801955005193 1105 18-19 0.3366037443643606 1105 20-21 0.35777076805448615 1105 22-23 0.39957926105370234 1105 24-25 0.40448199366065296 1105 26-27 0.361174060671658 1105 28-29 0.3590429454354833 1105 30-31 0.35811054164408773 1105 32-33 0.36016313165860225 1105 34-35 0.36973261803518653 1105 36-37 0.36918951121549526 1105 38-39 0.3685200916350553 1105 40-41 0.38154542726184815 1105 42-43 0.39748572987704733 1105 44-45 0.40620097861170024 1105 46-47 0.42223699825772343 1105 48-49 0.44344506467749767 1105 50-51 0.3275231257188622 1105 52-53 0.38143683732954514 1105 54-55 0.5075031206759348 1105 56-57 0.5384279624732784 1105 58-59 0.4987509842076179 1105 60-61 0.48140940374764085 1105 62-63 0.45929728118915847 1105 64-65 0.43029314719574785 1105 66-67 0.40527681088507705 1105 68-69 0.40850574457119393 1105 70-71 0.3894226172198785 1105 72-73 0.3995736250487951 1105 74-75 0.383167605153794 1105 76-77 0.38509899280263227 1105 78-79 0.40852377612683455 1105 80-81 0.36406793244775315 1105 82-83 0.38996988151357925 1105 84-85 0.3594771400394663 1105 86-87 0.30060388361905055 1105 88-89 0.35585243041778725 1105 90-91 0.3434625073472226 1105 92-93 0.30099082999022997 1105 94-95 0.3886931018652895 1105 96-97 0.3524350501618372 1105 98-99 0.2926853169217729 1105 100 0.30610815550498316 1106 1 0.23228451699486286 1106 2 0.2820101781029116 1106 3 0.25726478607094094 1106 4 0.24672785794016505 1106 5 0.2529992542760624 1106 6 0.229905521493734 1106 7 0.223053919185773 1106 8 0.24176727794881003 1106 9 0.25543575744615055 1106 10-11 0.22638825960762432 1106 12-13 0.23507910173275803 1106 14-15 0.31543622841558516 1106 16-17 0.286286382502027 1106 18-19 0.2745433104319446 1106 20-21 0.28971677666265094 1106 22-23 0.27724052470625793 1106 24-25 0.28469400695006897 1106 26-27 0.31546014511307163 1106 28-29 0.32217068513409686 1106 30-31 0.2953402221708501 1106 32-33 0.3319110762824451 1106 34-35 0.351450985310791 1106 36-37 0.2496524187763285 1106 38-39 0.2957714237968361 1106 40-41 0.29137324346553584 1106 42-43 0.317089474213347 1106 44-45 0.3525165726937516 1106 46-47 0.34891617672453634 1106 48-49 0.3531476166593208 1106 50-51 0.24801534761400745 1106 52-53 0.2828967057017948 1106 54-55 0.3780517503790435 1106 56-57 0.4424104200541805 1106 58-59 0.35387843656912565 1106 60-61 0.36634454873305344 1106 62-63 0.36018787348957204 1106 64-65 0.3257393861682445 1106 66-67 0.35569044466846833 1106 68-69 0.3467929374780283 1106 70-71 0.35682328426903354 1106 72-73 0.3397998729473741 1106 74-75 0.33342640412721636 1106 76-77 0.28645561637569017 1106 78-79 0.2903194355290779 1106 80-81 0.25677072858157857 1106 82-83 0.30587899486080516 1106 84-85 0.3077548358432338 1106 86-87 0.21717971068588682 1106 88-89 0.24868435957789004 1106 90-91 0.2089280292348974 1106 92-93 0.20033685496422393 1106 94-95 0.23735757089602316 1106 96-97 0.25052693680234484 1106 98-99 0.2492516756036487 1106 100 0.2324167758397131 1107 1 0.21481228471095548 1107 2 0.24121453778210977 1107 3 0.2302664926662672 1107 4 0.21215393004327154 1107 5 0.21941848004247788 1107 6 0.19310253592497162 1107 7 0.20636502334180307 1107 8 0.20931806989900537 1107 9 0.23761837510320305 1107 10-11 0.21791470854797268 1107 12-13 0.21990866922899954 1107 14-15 0.25678022887871066 1107 16-17 0.2430349313509339 1107 18-19 0.22769376981286626 1107 20-21 0.2401443936943366 1107 22-23 0.256312669654875 1107 24-25 0.28709458394824594 1107 26-27 0.2773660189837628 1107 28-29 0.28676373669236455 1107 30-31 0.26073885531597085 1107 32-33 0.2107690357199843 1107 34-35 0.26488150182413506 1107 36-37 0.23958658791435994 1107 38-39 0.23765518933851837 1107 40-41 0.2526514879878121 1107 42-43 0.29333581719158985 1107 44-45 0.2870708535538071 1107 46-47 0.28157529664964187 1107 48-49 0.32560469347086496 1107 50-51 0.2272584038007821 1107 52-53 0.24781870847237286 1107 54-55 0.3293614391047939 1107 56-57 0.34151547422079886 1107 58-59 0.30357889906651536 1107 60-61 0.3207662251421368 1107 62-63 0.28988502943283123 1107 64-65 0.2910958117786109 1107 66-67 0.2789225592845739 1107 68-69 0.2394503513525592 1107 70-71 0.24286094636776312 1107 72-73 0.23318851846099875 1107 74-75 0.2544895125936719 1107 76-77 0.2722842486183623 1107 78-79 0.28688966058797405 1107 80-81 0.2641809561019528 1107 82-83 0.24377706388856524 1107 84-85 0.2504301254501442 1107 86-87 0.24655663124343974 1107 88-89 0.2404195677070753 1107 90-91 0.22875461837752908 1107 92-93 0.25545415212672395 1107 94-95 0.2633074503835573 1107 96-97 0.28419098764007344 1107 98-99 0.25911462688697284 1107 100 0.28509046667602433 1108 1 0.11709263951347637 1108 2 0.14257012519767542 1108 3 0.1491970826968796 1108 4 0.12628947340936492 1108 5 0.1402822463608686 1108 6 0.08897995042116946 1108 7 0.09894393912539812 1108 8 0.13017827428304685 1108 9 0.13910387938738467 1108 10-11 0.08859906785554728 1108 12-13 0.10277839795082144 1108 14-15 0.15651113607407297 1108 16-17 0.13369132467812506 1108 18-19 0.1367585613877722 1108 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-1.0615214991252238 2316 84-85 -1.1855219408660886 2316 86-87 -1.3769467560060598 2316 88-89 -1.2910860786874245 2316 90-91 -1.1701781857120253 2316 92-93 -1.0175637480769808 2316 94-95 -1.266264817821348 2316 96-97 -1.460005550299492 2316 98-99 -1.3338916912243413 2316 100 -1.1081489864526723 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 18429.0 3 4593.0 4 3179.0 5 2761.0 6 4830.0 7 24891.0 8 49396.0 9 58087.0 10 59153.0 11 54154.0 12 49773.0 13 50667.0 14 56500.0 15 66047.0 16 80189.0 17 97797.0 18 109457.0 19 123577.0 20 143175.0 21 172024.0 22 217193.0 23 301859.0 24 429447.0 25 530782.0 26 548568.0 27 503745.0 28 465405.0 29 472676.0 30 537478.0 31 652872.0 32 817046.0 33 1062805.0 34 1473278.0 35 2235315.0 36 3855991.0 37 7412551.0 38 9962514.0 39 3113760.0 40 6382.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 16.285570638568174 33.011837606537014 27.655830741627014 23.0467610132678 2 20.613355596976433 31.220702408675066 29.560368511185736 18.605573483162765 3 23.172308463256837 27.239471889167827 27.378190475769777 22.210029171805555 4 19.114381652052824 28.185972359865563 27.084354037641322 25.615291950440287 5 21.181165253544137 31.417434295490693 27.135711230936995 20.265689220028175 6 25.33857676766212 27.370117000792 27.396949955784578 19.894356275761307 7 20.848644993694602 27.38764150537083 31.31888465423459 20.444828846699977 8 24.327446014086927 27.135378156160055 27.68139617292106 20.855779656831956 9 24.197652167407128 26.46588196153124 28.523956118796484 20.812509752265147 10-11 20.477870049495213 29.51337329094047 29.49097055159074 20.517786107973574 12-13 20.526289194738833 27.313114683220384 29.314746735023018 22.845849387017765 14-15 20.514488641580378 31.709358045824747 27.205805881854616 20.570347430740256 16-17 22.76812827890062 27.198808621561675 27.157209322346215 22.875853777191494 18-19 20.576869493593914 29.384044269920274 27.228487492145746 22.810598744340062 20-21 22.715468606214234 29.51725300987492 27.175463215725117 20.591815168185732 22-23 22.91048535915942 27.275626133232915 29.213490570141516 20.60039793746615 24-25 20.73888484073189 29.447827583107326 26.895133581381796 22.918153994778983 26-27 20.64702375292108 27.42166790160387 31.301298734531112 20.63000961094394 28-29 20.74728515445993 27.417203746845363 31.255651832045352 20.579859266649354 30-31 20.751019577047177 27.263196457197992 31.318560687371043 20.66722327838378 32-33 20.82526324342023 27.353822441900594 31.186641220126347 20.634273094552828 34-35 20.844511160956486 27.530043140986105 31.013645882369588 20.61179981568782 36-37 20.720511078343755 27.48127053456238 31.109888722463115 20.68832966463075 38-39 20.744821703667736 27.388801223491072 31.11803090354279 20.748346169298397 40-41 20.864219318070873 27.409506336504837 31.00601884596689 20.720255499457405 42-43 20.88143899846944 27.441893687153247 31.02225008254645 20.65441723183086 44-45 20.9900297007011 27.48157063425082 30.924334962865885 20.6040647021822 46-47 20.8003788702327 27.574765401059036 30.947473922362555 20.67738180634571 48-49 20.952187277980812 27.56385296913192 30.75993332971286 20.724026423174404 50-51 20.950130813494415 27.62010127947861 30.7178112607042 20.711956646322776 52-53 20.952868101721613 27.81508082443191 30.626849741819328 20.60520133202715 54-55 21.22693790357442 27.9089374487319 30.09262160020829 20.77150304748539 56-57 21.544915500908036 28.3645869037671 29.31594988597081 20.774547709354056 58-59 21.637229650774344 28.73124278656028 28.869782739909493 20.761744822755883 60-61 21.79576345178469 28.99545990221979 28.48994722569922 20.7188294202963 62-63 21.829205372520537 29.114223249688887 28.38654628156477 20.670025096225807 64-65 21.956719541997842 29.169202899335357 28.2937815629024 20.580295995764402 66-67 22.048094657516472 29.041091556961568 28.39078837813303 20.520025407388932 68-69 22.175666138992344 28.8934738226623 28.50483894605392 20.426021092291435 70-71 22.279593884921802 28.792452980993865 28.58237418439783 20.3455789496865 72-73 22.473342976453008 28.73957078807879 28.499355760043667 20.28773047542454 74-75 22.638465493547027 28.788013756303616 28.388644050041588 20.184876700107772 76-77 22.893267009896796 28.865306080021846 28.11663442230394 20.12479248777742 78-79 23.140624032500604 28.977570946975277 27.81313058606908 20.06867443445504 80-81 23.397441167645052 29.055087620863944 27.56127191998959 19.986199291501418 82-83 23.58382313839522 29.150309250858392 27.323164716108284 19.942702894638106 84-85 23.74200819891152 29.145789886248714 27.19534787252406 19.916854042315705 86-87 23.92088809962003 29.105149817219182 27.109326586440936 19.86463549671985 88-89 24.140663972589664 29.105539875112612 26.969928341494487 19.783867810803233 90-91 24.36036497454021 29.055599035651667 26.85187580429851 19.732160185509613 92-93 24.555379103635083 29.04640509728888 26.76646857973108 19.63174721934496 94-95 24.77335328972303 29.037810257807518 26.654622366529747 19.534214085939713 96-97 24.91721847479825 28.996026282777056 26.560736739399953 19.52601850302474 98-99 25.08178085227138 28.977871319945486 26.478627459402443 19.461720368380693 100 25.165133852745804 28.998669249365765 26.375593817640276 19.46060308024816 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 63937.0 1 41728.0 2 16133.5 3 11851.0 4 10825.5 5 10888.0 6 11139.5 7 11081.5 8 11107.5 9 11382.0 10 12071.0 11 14598.0 12 97981.5 13 122288.0 14 64716.5 15 68582.5 16 77753.5 17 87389.0 18 96677.0 19 106715.5 20 121454.5 21 147027.5 22 183620.5 23 223138.5 24 260145.5 25 303019.0 26 351255.0 27 408101.5 28 490330.5 29 570230.5 30 625259.0 31 686434.0 32 755722.0 33 804912.5 34 850749.0 35 920265.5 36 984960.0 37 1022865.5 38 1072632.5 39 1116615.0 40 1138902.0 41 1188487.5 42 1254886.5 43 1259213.0 44 1220143.5 45 1209399.5 46 1207786.0 47 1197825.0 48 1179433.5 49 1158696.0 50 1138962.5 51 1118555.0 52 1092798.0 53 1040597.0 54 969595.0 55 897020.0 56 822864.0 57 744447.5 58 657422.5 59 556170.0 60 452563.0 61 365553.5 62 286862.0 63 213260.0 64 155935.5 65 113154.0 66 81071.5 67 60556.5 68 45949.5 69 34834.0 70 27144.5 71 22046.5 72 18815.0 73 15510.5 74 13061.0 75 12150.0 76 9817.0 77 6268.0 78 4168.0 79 3310.5 80 2715.0 81 2236.0 82 1887.5 83 1640.5 84 1428.0 85 1218.0 86 1002.0 87 859.0 88 753.5 89 670.5 90 593.5 91 480.5 92 403.5 93 368.5 94 306.0 95 245.0 96 211.5 97 170.0 98 134.0 99 100.0 100 55.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4010316300953441 2 0.3831379768410186 3 0.1681573578640778 4 0.17472478355545634 5 0.2719271495256856 6 0.10197791435864775 7 0.13591752184150505 8 0.14170902558549592 9 0.13452197877066388 10-11 0.1312019818051327 12-13 0.1329087309807715 14-15 0.13880908708428796 16-17 0.14318551015444586 18-19 0.15497087138769955 20-21 0.1358184382834753 22-23 0.09848347450926148 24-25 0.09376235230060578 26-27 0.08834904072881287 28-29 0.0982336722995809 30-31 0.10268126806635171 32-33 0.10392051031325868 34-35 0.10449686960151608 36-37 0.10033117353505518 38-39 0.09960270005207608 40-41 0.10053352728032715 42-43 0.06349302309406078 44-45 0.06643901451660648 46-47 0.08467318028021724 48-49 0.08900773705824991 50-51 0.09827832967784782 52-53 0.10336368862799304 54-55 0.10588403941393221 56-57 0.1065315713988025 58-59 0.1059175324476324 60-61 0.10370699222341996 62-63 0.10801922031231918 64-65 0.11493274068526635 66-67 0.12355440577692312 68-69 0.12887561150604052 70-71 0.12722328851016454 72-73 0.11395027836339418 74-75 0.11151226461863464 76-77 0.11358325053576294 78-79 0.11222957375704701 80-81 0.10649249619281895 82-83 0.10962828147299905 84-85 0.11521184929943458 86-87 0.12879187892179003 88-89 0.13674228779637218 90-91 0.14357207558506888 92-93 0.14293012577248193 94-95 0.13302037442643877 96-97 0.12939056689918088 98-99 0.12449081517745754 100 0.11869931143346668 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3.5828346E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.81939960221468 #Duplication Level Percentage of deduplicated Percentage of total 1 73.93490150944297 30.179782892643374 2 13.266272055811354 10.830425205557155 3 4.559020213263487 5.582894036393288 4 2.267776778303609 3.7027714608879214 5 1.3213865445110213 2.696910269469251 6 0.8731622452810124 2.138517516461558 7 0.6249275497698219 1.7856417163541083 8 0.4909756948168641 1.6033066465363663 9 0.36286728653007044 1.3330822294298048 >10 1.9659918332850725 14.953269339662278 >50 0.16988242619961305 4.747708193679703 >100 0.15228395051409818 13.518915813418438 >500 0.010089014685961576 2.4161590098077967 >1k 4.2869511693018747E-4 0.34452239114303634 >5k 0.0 0.0 >10k+ 3.4202468021804616E-5 4.166093278555812 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 1340501 3.7414537640113226 No Hit AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC 55591 0.1551592697022631 Illumina Single End PCR Primer 1 (100% over 50bp) AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 50286 0.1403525577206383 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.18246725651248316 2.791086141682343E-6 0.0 2.791086141682343E-6 5.582172283364686E-6 2 0.19457777928124284 1.1164344566729372E-5 0.0 2.791086141682343E-6 5.582172283364686E-6 3 0.2084159843717039 1.3955430708411713E-5 0.0 2.791086141682343E-6 5.582172283364686E-6 4 0.21146105935227935 2.7910861416823427E-5 0.0 2.791086141682343E-6 1.1164344566729372E-5 5 0.21190205096266515 3.349303370018811E-5 0.0 2.791086141682343E-6 1.3955430708411713E-5 6 0.2120834715618745 3.90752059835528E-5 0.0 8.373258425047028E-6 1.95376029917764E-5 7 0.21237932669289283 4.186629212523514E-5 0.0 1.1164344566729372E-5 2.2328689133458743E-5 8 0.21331434055035642 4.186629212523514E-5 0.0 1.1164344566729372E-5 2.7910861416823427E-5 9 0.21427168309695346 4.465737826691749E-5 0.0 1.1164344566729372E-5 3.349303370018811E-5 10-11 0.2146024268047428 5.7217265904488035E-5 0.0 1.5350973779252885E-5 3.628411984187046E-5 12-13 0.21474477219796861 7.39637827545821E-5 0.0 1.95376029917764E-5 5.023955055028217E-5 14-15 0.21495968583087816 7.81504119671056E-5 2.791086141682343E-6 2.2328689133458743E-5 6.698606740037623E-5 16-17 0.21519692815292113 7.954595503794677E-5 2.791086141682343E-6 2.651531834598226E-5 7.396378275458208E-5 18-19 0.21533648246000525 8.094149810878794E-5 2.791086141682343E-6 2.93064044876646E-5 7.954595503794677E-5 20-21 0.21544393927646005 8.512812732131146E-5 2.791086141682343E-6 3.7679662912711625E-5 8.931475653383496E-5 22-23 0.21556953815283575 9.908355802972318E-5 2.791086141682343E-6 4.186629212523514E-5 9.629247188804083E-5 24-25 0.21588074425763332 1.0745681645477019E-4 2.791086141682343E-6 5.0239550550282173E-5 1.0187464417140552E-4 26-27 0.21682692245966362 1.1862116102149956E-4 2.791086141682343E-6 5.582172283364685E-5 1.0327018724224668E-4 28-29 0.2197575629084301 1.269944194465466E-4 2.791086141682343E-6 5.86128089753292E-5 1.0885235952561137E-4 30-31 0.22735908601530197 1.367632209424348E-4 2.791086141682343E-6 6.279943818785272E-5 1.2001670409234074E-4 32-33 0.2425356169106997 1.4792756550916416E-4 2.791086141682343E-6 7.39637827545821E-5 1.2978550558822893E-4 34-35 0.26996641151115375 1.5909191007589354E-4 2.791086141682343E-6 8.373258425047029E-5 1.353676778715936E-4 36-37 0.313448742512423 1.6188299621757587E-4 2.791086141682343E-6 8.931475653383497E-5 1.423453932257995E-4 38-39 0.3765189160560189 1.646740823592582E-4 2.791086141682343E-6 8.931475653383497E-5 1.507186516508465E-4 40-41 0.46158005730992996 1.7165179771346406E-4 2.791086141682343E-6 9.629247188804083E-5 1.5490528086337005E-4 42-43 0.5709990073223028 1.8421168535103462E-4 2.791086141682343E-6 1.0187464417140552E-4 1.646740823592582E-4 44-45 0.7084767463170083 1.8560722842187577E-4 2.791086141682343E-6 1.0466573031308785E-4 1.8281614228019347E-4 46-47 0.8804523100229076 1.95376029917764E-4 2.791086141682343E-6 1.0745681645477019E-4 1.9258494377608163E-4 48-49 1.0870499017733053 1.9816711605944633E-4 2.791086141682343E-6 1.1303898873813489E-4 2.0374928834281103E-4 50-51 1.3299888306314782 2.0095820220112867E-4 2.791086141682343E-6 1.1443453180897606E-4 2.1072700369701689E-4 52-53 1.6054676372724548 2.051448314136522E-4 2.791086141682343E-6 1.172256179506584E-4 2.1910026212206392E-4 54-55 1.9179004244293054 2.2189134826374626E-4 2.791086141682343E-6 1.172256179506584E-4 2.246824344054286E-4 56-57 2.274747765358747 2.3166014975963445E-4 2.791086141682343E-6 1.214122471631819E-4 2.3026460668879327E-4 58-59 2.674989518075995 2.3584677897215797E-4 2.791086141682343E-6 1.2838996251738778E-4 2.4142895125552267E-4 60-61 3.1201677576743285 2.4701112353888734E-4 2.791086141682343E-6 1.2838996251738778E-4 2.539888388930932E-4 62-63 3.5976737525086984 2.581754681056167E-4 2.791086141682343E-6 1.3397213480075248E-4 2.665487265306637E-4 64-65 4.113178989619001 2.735264418848696E-4 2.791086141682343E-6 1.4094985015495834E-4 2.791086141682343E-4 66-67 4.669816463199278 2.7631752802655194E-4 2.791086141682343E-6 1.423453932257995E-4 2.8887741566412247E-4 68-69 5.2671423905529995 2.791086141682343E-4 2.791086141682343E-6 1.4374093629664064E-4 2.944595879474872E-4 70-71 5.900852916849692 2.860863295224401E-4 2.791086141682343E-6 1.4513647936748183E-4 3.000417602308518E-4 72-73 6.571828629767056 3.000417602308518E-4 2.791086141682343E-6 1.4513647936748183E-4 3.1399719093926353E-4 74-75 7.273979379343942 3.112061047975812E-4 2.791086141682343E-6 1.46532022438323E-4 3.2097490629346944E-4 76-77 8.008674193332844 3.265570785768341E-4 2.791086141682343E-6 1.4792756550916416E-4 3.2516153550599293E-4 78-79 8.774906885179684 3.293481647185165E-4 2.791086141682343E-6 1.507186516508465E-4 3.293481647185164E-4 80-81 9.57223925436022 3.4330359542692813E-4 2.791086141682343E-6 1.5490528086337005E-4 3.321392508601988E-4 82-83 10.398596128328112 3.558634830644987E-4 4.186629212523514E-6 1.563008239342112E-4 3.3911696621440464E-4 84-85 11.24988298371351 3.6284119841870457E-4 5.582172283364686E-6 1.563008239342112E-4 3.433035954269282E-4 86-87 12.12871506823117 3.698189137729104E-4 5.582172283364686E-6 1.6467408235925824E-4 3.460946815686105E-4 88 12.798455725530841 3.712144568437516E-4 5.582172283364686E-6 1.6467408235925824E-4 3.5167685385197517E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 173070 0.0 85.16639 8 TGGTATC 175520 0.0 83.70725 7 AGTGGTA 177045 0.0 83.45701 5 GTGGTAT 177600 0.0 83.1168 6 GTATCAA 177610 0.0 82.92609 9 GCAGTGG 186230 0.0 79.794174 3 AAGCAGT 187515 0.0 79.2266 1 CAGTGGT 190190 0.0 77.63012 4 AGCAGTG 199970 0.0 74.42123 2 CAACGCA 168220 0.0 44.837357 12-13 ATCAACG 170040 0.0 44.421036 10-11 ACGCAGA 171830 0.0 43.96153 14-15 TCAACGC 167910 0.0 43.924904 12-13 AACGCAG 170000 0.0 43.53876 14-15 AGAGTAC 173020 0.0 43.093246 18-19 CGCAGAG 173585 0.0 42.566936 16-17 GAGTACT 170430 0.0 42.49249 20-21 TATCAAC 175795 0.0 41.81448 10-11 AGTACTT 181550 0.0 40.77139 20-21 GTACTTT 182555 0.0 39.655758 22-23 >>END_MODULE