FastQCFastQC Report
Thu 26 May 2016
SRR1660733_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660733_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35828346
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT13486723.764259728875009No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAGGTATATCTCGTA536970.14987295254991678TruSeq Adapter, Index 5 (97% over 37bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA381420.10645760761604792No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1810400.084.933428
TGGTATC1834850.083.737747
AGTGGTA1841350.082.890535
GTATCAA1854350.082.8190469
GTGGTAT1844450.082.728316
GCAGTGG1892200.080.637583
AAGCAGT1846750.080.33721
CAGTGGT1961900.077.654244
AGCAGTG2019900.075.222952
CAACGCA1760150.044.5269912-13
ATCAACG1779450.044.11002710-11
ACGCAGA1796650.043.69554514-15
TCAACGC1755150.043.69507612-13
AACGCAG1779150.043.2085414-15
AGAGTAC1830000.042.7739418-19
CGCAGAG1806700.042.55238316-17
GAGTACT1800700.042.15364520-21
TATCAAC1845800.041.58223710-11
AGTACTT1909650.040.64834220-21
GTACTTT1917550.039.5918622-23
Produced by FastQC (version 0.11.5)