##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660733_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 35828346 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.193446691622327 34.0 31.0 34.0 27.0 34.0 2 31.850481096727155 34.0 31.0 34.0 28.0 34.0 3 31.62495363308147 34.0 31.0 34.0 30.0 34.0 4 35.55627809332867 37.0 35.0 37.0 33.0 37.0 5 35.89556414354154 37.0 35.0 37.0 33.0 37.0 6 35.35966446790483 37.0 35.0 37.0 33.0 37.0 7 35.59490513461046 37.0 35.0 37.0 35.0 37.0 8 35.94959878974039 37.0 35.0 37.0 35.0 37.0 9 37.76193634503809 39.0 38.0 39.0 35.0 39.0 10-11 37.801350807542164 39.0 37.5 39.0 35.0 39.0 12-13 37.84282919451543 39.0 38.0 39.0 35.0 39.0 14-15 39.28575566954723 41.0 39.0 41.0 36.0 41.0 16-17 39.25341598241794 41.0 39.0 41.0 36.0 41.0 18-19 39.253298519557674 41.0 39.0 41.0 36.0 41.0 20-21 39.20946307429318 40.5 39.0 41.0 36.0 41.0 22-23 39.119383657844544 40.0 39.0 41.0 35.5 41.0 24-25 39.11305305581229 40.0 39.0 41.0 36.0 41.0 26-27 39.04230104565809 40.0 39.0 41.0 35.5 41.0 28-29 38.95348904747096 40.0 39.0 41.0 35.0 41.0 30-31 38.85620893579625 40.0 38.0 41.0 35.0 41.0 32-33 38.73740286531787 40.0 38.0 41.0 35.0 41.0 34-35 38.658467530150574 40.0 38.0 41.0 35.0 41.0 36-37 38.55231805844456 40.0 38.0 41.0 34.0 41.0 38-39 38.246416929210184 40.0 38.0 41.0 34.0 41.0 40-41 38.10474347601756 40.0 37.0 41.0 33.5 41.0 42-43 37.95852674862523 40.0 37.0 41.0 33.0 41.0 44-45 37.83477688308581 40.0 37.0 41.0 33.0 41.0 46-47 37.69344524025753 40.0 37.0 41.0 33.0 41.0 48-49 37.75143902819293 40.0 37.0 41.0 33.0 41.0 50-51 37.77378391958144 40.0 37.0 41.0 33.0 41.0 52-53 37.580554821034724 40.0 36.5 41.0 33.0 41.0 54-55 37.19228234817203 40.0 36.0 41.0 31.5 41.0 56-57 36.83092257454474 40.0 36.0 41.0 31.0 41.0 58-59 36.73282188354439 39.5 36.0 41.0 31.0 41.0 60-61 36.595409804851165 39.0 35.0 41.0 31.0 41.0 62-63 36.3638612985372 39.0 35.0 41.0 30.0 41.0 64-65 36.107400101584375 38.5 35.0 41.0 30.0 41.0 66-67 35.82329565255399 38.0 35.0 40.0 29.5 41.0 68-69 35.54573444445356 37.0 35.0 40.0 29.0 41.0 70-71 35.23262779141409 37.0 35.0 39.5 29.0 41.0 72-73 34.86663950381633 36.0 34.0 39.0 29.0 41.0 74-75 34.463833594216155 36.0 34.0 39.0 28.5 40.5 76-77 32.74602757269342 34.5 32.0 36.5 25.5 39.0 78-79 33.6843710424143 35.0 34.0 37.0 27.5 39.0 80-81 33.58487863213111 35.0 34.0 37.0 28.0 39.0 82-83 33.32604573764024 35.0 34.0 36.0 27.5 38.5 84-85 33.07242223796767 35.0 34.0 36.0 27.0 37.0 86-87 32.85451184377867 35.0 34.0 35.5 27.0 37.0 88-89 32.67884640837174 35.0 34.0 35.0 27.0 36.5 90-91 32.518835616916284 35.0 34.0 35.0 26.0 36.0 92-93 32.376490698175125 35.0 33.0 35.0 26.0 36.0 94-95 32.27076601582445 35.0 33.0 35.0 25.5 36.0 96-97 32.174614954315786 35.0 33.0 35.0 25.0 35.5 98-99 32.08888774826502 35.0 33.0 35.0 25.0 35.0 100 32.03943986138796 35.0 33.0 35.0 25.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.5856893438642103 1101 2 1.1878908225822613 1101 3 1.5401610096798528 1101 4 0.9463164173700207 1101 5 0.5667581823292167 1101 6 1.1147544168125663 1101 7 0.8077591988343329 1101 8 0.4903950634838381 1101 9 0.5033314349907769 1101 10-11 0.4112286876518425 1101 12-13 0.3879693811380349 1101 14-15 0.49396111966782996 1101 16-17 0.5250820689639468 1101 18-19 0.5053072214752348 1101 20-21 0.49652169499609045 1101 22-23 0.480400742054222 1101 24-25 0.48344337049932307 1101 26-27 0.48414996326475546 1101 28-29 0.49701562319697956 1101 30-31 0.4762540973602043 1101 32-33 0.5200203702003279 1101 34-35 0.541166707937002 1101 36-37 0.4934278317249934 1101 38-39 0.5268900011178559 1101 40-41 0.5014583539433559 1101 42-43 0.4982160521791741 1101 44-45 0.5077030850937163 1101 46-47 0.5099497875589378 1101 48-49 0.4690237392363841 1101 50-51 0.4422313339161761 1101 52-53 0.4459329183547851 1101 54-55 0.4336755728204267 1101 56-57 0.45437715619106456 1101 58-59 0.4942538067552107 1101 60-61 0.49886997585958426 1101 62-63 0.47413327881832146 1101 64-65 0.44052428499211516 1101 66-67 0.4618494407657252 1101 68-69 0.42105557667438376 1101 70-71 0.42364437682012124 1101 72-73 0.420000343729221 1101 74-75 0.4146775777720819 1101 76-77 0.3291097165482242 1101 78-79 0.4397822382064547 1101 80-81 0.4538128756226101 1101 82-83 0.43423348168463605 1101 84-85 0.39024191173239586 1101 86-87 0.38090939801403323 1101 88-89 0.3647804884640351 1101 90-91 0.3851972965843302 1101 92-93 0.3835813251650535 1101 94-95 0.35616666387889495 1101 96-97 0.3549574977222605 1101 98-99 0.35944823244462754 1101 100 0.33901244582012424 1102 1 1.5409160645603137 1102 2 1.1648476011748343 1102 3 1.5144336609696971 1102 4 0.9128031289953071 1102 5 0.5171015922144946 1102 6 1.0900098985338929 1102 7 0.7821534366677696 1102 8 0.4434249695267951 1102 9 0.48198231252593615 1102 10-11 0.3906812448117094 1102 12-13 0.371051155065274 1102 14-15 0.47727199610217497 1102 16-17 0.5034421530430606 1102 18-19 0.45490260101662017 1102 20-21 0.4373225635081326 1102 22-23 0.44332582798306674 1102 24-25 0.4535314703502209 1102 26-27 0.47125482562859133 1102 28-29 0.47729682276848706 1102 30-31 0.4791270808528978 1102 32-33 0.4734077520854143 1102 34-35 0.4495900615559023 1102 36-37 0.45040709229207465 1102 38-39 0.4715119388805178 1102 40-41 0.45240179907922595 1102 42-43 0.45904926637076926 1102 44-45 0.43888501060632024 1102 46-47 0.46847136698099945 1102 48-49 0.42140118260155646 1102 50-51 0.3772394901853673 1102 52-53 0.3728465800447509 1102 54-55 0.43232934385905963 1102 56-57 0.4723857451703566 1102 58-59 0.4586390094104331 1102 60-61 0.45984615187811784 1102 62-63 0.42741829578856283 1102 64-65 0.3970796515155328 1102 66-67 0.3957391903862728 1102 68-69 0.3880645888927603 1102 70-71 0.35440195367145577 1102 72-73 0.3364884221794142 1102 74-75 0.3458655564135569 1102 76-77 0.17980753046959563 1102 78-79 0.395221843779602 1102 80-81 0.4453255930812716 1102 82-83 0.42703641382158253 1102 84-85 0.4084640851274415 1102 86-87 0.3978410683292992 1102 88-89 0.3985857607764913 1102 90-91 0.42066905835064716 1102 92-93 0.39731559058820665 1102 94-95 0.37102648049204845 1102 96-97 0.40663513499428205 1102 98-99 0.41439160374530815 1102 100 0.3818297114008047 1103 1 1.5110963859572308 1103 2 1.1242878418619782 1103 3 1.4544298646949692 1103 4 0.9229169320987864 1103 5 0.5352123180487709 1103 6 1.0579822611593386 1103 7 0.7808778402514562 1103 8 0.4353141108131098 1103 9 0.4633030850032114 1103 10-11 0.37434471155737725 1103 12-13 0.3449050244154108 1103 14-15 0.42795377304886273 1103 16-17 0.4371344896917151 1103 18-19 0.40217314289854045 1103 20-21 0.4079759349870926 1103 22-23 0.37819866290941917 1103 24-25 0.3953918571078887 1103 26-27 0.41779919776301 1103 28-29 0.397990460809055 1103 30-31 0.41130092138258334 1103 32-33 0.4468331985753764 1103 34-35 0.4422844453247734 1103 36-37 0.39281124105283993 1103 38-39 0.41883185302169323 1103 40-41 0.44164203281452785 1103 42-43 0.46111481434461155 1103 44-45 0.4314072127142339 1103 46-47 0.46270776854394313 1103 48-49 0.42171995699730047 1103 50-51 0.41945938521234893 1103 52-53 0.4161352408021557 1103 54-55 0.4551402350201741 1103 56-57 0.5102467426714128 1103 58-59 0.5068110144415385 1103 60-61 0.4720850737551956 1103 62-63 0.4306132100926945 1103 64-65 0.43247488827901037 1103 66-67 0.44723859128743015 1103 68-69 0.40076976386509955 1103 70-71 0.4106320680858886 1103 72-73 0.4176872430647123 1103 74-75 0.4327072029819874 1103 76-77 0.27437637323763653 1103 78-79 0.44982603387804687 1103 80-81 0.46974239860875144 1103 82-83 0.4609633025182944 1103 84-85 0.452412661077922 1103 86-87 0.4287666788068947 1103 88-89 0.4170971584235943 1103 90-91 0.416540822278769 1103 92-93 0.42930335801956687 1103 94-95 0.41129104523048454 1103 96-97 0.44087505844301234 1103 98-99 0.4541050326221736 1103 100 0.43728873199411566 1104 1 1.437822319658224 1104 2 1.0577546467664476 1104 3 1.4117170618508332 1104 4 0.871541603146099 1104 5 0.49048536581822333 1104 6 1.0093432299024059 1104 7 0.7449175895337561 1104 8 0.4055442284578845 1104 9 0.4132843942387723 1104 10-11 0.31020633602459213 1104 12-13 0.30909065932566193 1104 14-15 0.3587301955562836 1104 16-17 0.36418765091417526 1104 18-19 0.3375984926194988 1104 20-21 0.3425191489607684 1104 22-23 0.3246988768245487 1104 24-25 0.3189265866938271 1104 26-27 0.3284008449328226 1104 28-29 0.31937032623503825 1104 30-31 0.33069487395014363 1104 32-33 0.35376737789368917 1104 34-35 0.38213806384420224 1104 36-37 0.35298136124791313 1104 38-39 0.36779586461027947 1104 40-41 0.3314114878803309 1104 42-43 0.3853831299295081 1104 44-45 0.3089569543808395 1104 46-47 0.3484650726684251 1104 48-49 0.28789147386103764 1104 50-51 0.27387105848690396 1104 52-53 0.2907051023320264 1104 54-55 0.3060572033541149 1104 56-57 0.33675676873351534 1104 58-59 0.3549053973406515 1104 60-61 0.3278755795439281 1104 62-63 0.2664583482048286 1104 64-65 0.25892454955184263 1104 66-67 0.2974342368745724 1104 68-69 0.24299389063290988 1104 70-71 0.2591065674478159 1104 72-73 0.27249609919710593 1104 74-75 0.26934668105567994 1104 76-77 0.16896671432707677 1104 78-79 0.28546575428939747 1104 80-81 0.29849422328437925 1104 82-83 0.3323325639726491 1104 84-85 0.31532681006858354 1104 86-87 0.2930411345167343 1104 88-89 0.29825978065940006 1104 90-91 0.2823165756226871 1104 92-93 0.281757377856259 1104 94-95 0.2737215962844459 1104 96-97 0.2783540391844497 1104 98-99 0.2946726631529941 1104 100 0.323103978590062 1105 1 1.3796431907455045 1105 2 1.0114382186208886 1105 3 1.3557806779683865 1105 4 0.8362523068034733 1105 5 0.4376726397735169 1105 6 0.9880355612038443 1105 7 0.712019972477961 1105 8 0.3571610753411463 1105 9 0.3649951443005932 1105 10-11 0.2501186127577526 1105 12-13 0.23513766200132125 1105 14-15 0.30417565455331186 1105 16-17 0.3000429801006277 1105 18-19 0.28477166634519335 1105 20-21 0.28756687666297864 1105 22-23 0.27626722905446854 1105 24-25 0.30458372744438833 1105 26-27 0.31959944148191255 1105 28-29 0.2872577739183413 1105 30-31 0.28890758468595834 1105 32-33 0.34133393791518074 1105 34-35 0.34318132423288006 1105 36-37 0.3380410274191945 1105 38-39 0.3490926749403158 1105 40-41 0.36594480998486745 1105 42-43 0.33683796704873714 1105 44-45 0.32665735665067785 1105 46-47 0.34501322375763976 1105 48-49 0.36649788188007903 1105 50-51 0.3223602470845819 1105 52-53 0.33338530867116845 1105 54-55 0.36781305550805143 1105 56-57 0.3654323445655976 1105 58-59 0.32402569959754146 1105 60-61 0.29939327924090264 1105 62-63 0.2792464452637162 1105 64-65 0.24671763075635766 1105 66-67 0.28530892725033397 1105 68-69 0.2474392286870284 1105 70-71 0.2679887644567316 1105 72-73 0.24368536194089785 1105 74-75 0.24658272272861836 1105 76-77 0.1344841888456827 1105 78-79 0.2811026864032371 1105 80-81 0.30342173540710604 1105 82-83 0.3028894340551602 1105 84-85 0.2856556606497591 1105 86-87 0.3058555881552252 1105 88-89 0.300483508300168 1105 90-91 0.2890838826193729 1105 92-93 0.27714522806529374 1105 94-95 0.24981541835002474 1105 96-97 0.2680642868781362 1105 98-99 0.29724308135740074 1105 100 0.29134812438047675 1106 1 1.3547078979009228 1106 2 0.98224060203097 1106 3 1.33339185403673 1106 4 0.7979489289482942 1106 5 0.42242698166332815 1106 6 0.9468563690157836 1106 7 0.6798730189071875 1106 8 0.3246922972032422 1106 9 0.30811172378776774 1106 10-11 0.22179918778116559 1106 12-13 0.18642708771779581 1106 14-15 0.22713301243732076 1106 16-17 0.2209114663756182 1106 18-19 0.1790337555123358 1106 20-21 0.22232313630382805 1106 22-23 0.2441251039110739 1106 24-25 0.24032032435368933 1106 26-27 0.19525742433516768 1106 28-29 0.2076167319654374 1106 30-31 0.23311232593854214 1106 32-33 0.2743944931445981 1106 34-35 0.2756308815923276 1106 36-37 0.25761599676997093 1106 38-39 0.28586482501703614 1106 40-41 0.29839576587745853 1106 42-43 0.2792076658901692 1106 44-45 0.280852972856529 1106 46-47 0.2820381084558008 1106 48-49 0.26461739121113936 1106 50-51 0.2657807222595707 1106 52-53 0.24853604458313328 1106 54-55 0.29712484638385916 1106 56-57 0.3056648829566342 1106 58-59 0.2995283892973504 1106 60-61 0.31311447933674685 1106 62-63 0.2842121439794596 1106 64-65 0.24785947659508167 1106 66-67 0.22963358524561528 1106 68-69 0.24220013791907036 1106 70-71 0.22306489638220484 1106 72-73 0.15814000206474788 1106 74-75 0.21619327146017042 1106 76-77 0.0964549288847465 1106 78-79 0.24547716026535227 1106 80-81 0.26149482586182415 1106 82-83 0.2737350619537082 1106 84-85 0.2529152083134534 1106 86-87 0.24930123568107376 1106 88-89 0.253608389593019 1106 90-91 0.26695355187735714 1106 92-93 0.2650826388637597 1106 94-95 0.22882220010964005 1106 96-97 0.27428710951325996 1106 98-99 0.26656562776694415 1106 100 0.26495460328424514 1107 1 1.313747063536269 1107 2 0.9264021298029306 1107 3 1.2867785756759922 1107 4 0.7889516783825528 1107 5 0.3963115265712034 1107 6 0.9454576294905976 1107 7 0.6638159083930333 1107 8 0.29690976668875635 1107 9 0.2795093754296474 1107 10-11 0.18790325214268222 1107 12-13 0.15234203588634898 1107 14-15 0.19114327308602697 1107 16-17 0.1794922209677594 1107 18-19 0.15706507190927255 1107 20-21 0.155671648294593 1107 22-23 0.18203407539602523 1107 24-25 0.17032822810866577 1107 26-27 0.15035522716284788 1107 28-29 0.15936069121752183 1107 30-31 0.14864555300479765 1107 32-33 0.19737437784569067 1107 34-35 0.2153321950478997 1107 36-37 0.17744692979907484 1107 38-39 0.1956595587564962 1107 40-41 0.201013990875623 1107 42-43 0.22048248806900972 1107 44-45 0.1912130688831013 1107 46-47 0.2203002905676854 1107 48-49 0.2092451248227647 1107 50-51 0.20481549727308845 1107 52-53 0.18082671981597542 1107 54-55 0.1782733258577167 1107 56-57 0.17601101364232363 1107 58-59 0.15615901668121523 1107 60-61 0.15427095108611866 1107 62-63 0.14779820528503507 1107 64-65 0.15459223935895494 1107 66-67 0.17360784051680156 1107 68-69 0.17486206748343136 1107 70-71 0.18202818466114934 1107 72-73 0.13929094862802316 1107 74-75 0.1498198755688449 1107 76-77 0.022475444358498464 1107 78-79 0.16553192102430359 1107 80-81 0.22151090124140893 1107 82-83 0.20631455458069325 1107 84-85 0.19726812571210672 1107 86-87 0.17690295675875944 1107 88-89 0.20704336079311503 1107 90-91 0.2196129577321173 1107 92-93 0.20264092610464957 1107 94-95 0.17869314682354798 1107 96-97 0.21288811173905486 1107 98-99 0.2304609838449636 1107 100 0.21174295142900235 1108 1 1.2479233414945696 1108 2 0.8896911609783302 1108 3 1.2282580187134222 1108 4 0.747238583859918 1108 5 0.33763093906290464 1108 6 0.8769790434428018 1108 7 0.612042992084028 1108 8 0.2635945885391564 1108 9 0.2044350025201993 1108 10-11 0.12619565333160665 1108 12-13 0.11852183909877567 1108 14-15 0.1522026761416484 1108 16-17 0.15389694585987002 1108 18-19 0.10481076729399064 1108 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-1.4302817663715288 2316 84-85 -1.0320545715384881 2316 86-87 -0.8373043150674988 2316 88-89 -0.7238145850301834 2316 90-91 -0.791075694587505 2316 92-93 -0.8875752542136865 2316 94-95 -0.9199534488563472 2316 96-97 -0.9967956270299538 2316 98-99 -1.137665808540973 2316 100 -1.0538170435274523 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 82.0 7 1108.0 8 1265.0 9 549.0 10 413.0 11 878.0 12 1168.0 13 1655.0 14 3423.0 15 7892.0 16 15614.0 17 24423.0 18 33117.0 19 44928.0 20 62026.0 21 86496.0 22 128468.0 23 207608.0 24 351683.0 25 482414.0 26 502442.0 27 445843.0 28 416110.0 29 457071.0 30 544492.0 31 654152.0 32 800966.0 33 1023734.0 34 1404373.0 35 2150851.0 36 3851785.0 37 7908603.0 38 1.093425E7 39 3271291.0 40 7173.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 17.052107395930637 32.60917589486365 27.821623919793474 22.517092789412235 2 20.865880869958907 31.418799611232718 29.335799136159547 18.37952038264883 3 23.255420382598015 27.3874599767234 27.520557764462755 21.836561876215825 4 19.444439327412052 28.24085804483179 27.12404897859661 25.19065364915955 5 21.13259637282036 31.574918654462657 27.142482459343004 20.150002513373984 6 26.22094033326656 27.23517732853046 27.06076126853078 19.483121069672187 7 20.75796747395377 27.387919826218255 31.510639018264776 20.3434736815632 8 24.397458541083793 27.104404235318285 27.689911645115693 20.808225578482226 9 24.33882446984364 26.413017402841245 28.50042892017479 20.747729207140324 10-11 20.398023128072985 29.56429299503856 29.607218535504877 20.430465341383574 12-13 20.43201073250772 27.25856644345234 29.426245353330017 22.883177470709924 14-15 20.29467812999238 31.943932047546937 27.120388141836077 20.64100168062461 16-17 22.68891507238614 27.243053577566474 27.143806710526736 22.92422463952065 18-19 20.44446037224269 29.41734318408112 27.262324640942122 22.875871802734068 20-21 22.706139000685035 29.436318706586523 27.213853566071016 20.643688726657427 22-23 22.786483613024462 27.31514964190909 29.359561031197323 20.538805713869124 24-25 20.510995597987236 29.506254801391396 26.918510681934066 23.064238918687302 26-27 20.557469509210343 27.169283455191078 31.59751505910074 20.67573197649784 28-29 20.5710833406348 27.325790106815035 31.457600354735426 20.64552619781474 30-31 20.672863755041096 27.202358424695248 31.510867983612144 20.613909836651505 32-33 20.68348832467942 27.367400708819396 31.276026628455256 20.673084338045932 34-35 20.67519547432823 27.361000476178766 31.19340492269862 20.770399126794384 36-37 20.634069863825278 27.399835029979684 31.21577752362395 20.750317582571085 38-39 20.857215409250156 27.307272200364157 31.21947063298942 20.61604175739626 40-41 20.710284814379815 27.42950777317269 31.302416644668735 20.557790767778766 42-43 20.6998988749872 27.47380494512186 31.221126717922377 20.605169461968565 44-45 20.730785191627994 27.4194735908826 31.18837475436595 20.66136646312346 46-47 20.76108350081273 27.496305737050896 31.102694004575454 20.63991675756092 48-49 20.884614783753126 27.505372108632685 31.010453715666646 20.59955939194754 50-51 20.792558583951347 27.68139980355383 30.938967591687383 20.58707402080743 52-53 20.972691218396143 27.70262272011793 30.63773656639312 20.686949495092804 54-55 21.224403431358056 27.85428329105239 30.0955389871554 20.825774290434154 56-57 21.439809956011445 28.257406054384436 29.358385320674735 20.944398668929384 58-59 21.477637192463373 28.67192973530493 28.945846028827187 20.90458704340451 60-61 21.52779591340345 28.872420724952104 28.569610250977256 21.030173110667196 62-63 21.607208543843186 28.996971304699155 28.345171988440672 21.050648163016987 64-65 21.54088045923989 29.091851887428373 28.423073185633285 20.944194467698455 66-67 21.616140080599315 29.11040495198451 28.30853899409254 20.964915973323635 68-69 21.500949325475005 29.12356333033167 28.387254199317663 20.98823314487566 70-71 21.456404944653112 29.089080722718396 28.436848060756297 21.017666271872194 72-73 21.45953695876028 29.054698522798077 28.3530421323689 21.132722386072743 74-75 21.510413896461237 29.10026790442139 28.222912587184627 21.166405611932742 76-77 21.556597201363754 29.188380072085195 28.004856172977437 21.250166553573614 78-79 21.629991894877225 29.308580261108467 27.687679977408923 21.373747866605378 80-81 21.706647877692422 29.4232010730961 27.40156746557786 21.46858358363362 82-83 21.70831892665655 29.535085952220975 27.133001695403603 21.623593425718877 84-85 21.704121763099277 29.536221157635893 26.98289691182901 21.77676016743582 86-87 21.71829400008286 29.51656357621138 26.869157844287212 21.89598457941855 88-89 21.722865840596604 29.555993439358275 26.71683400719575 22.004306712849374 90-91 21.76997715982377 29.47674959708695 26.580929720080242 22.172343523009037 92-93 21.77911414325896 29.4724209465244 26.494558329976485 22.253906580240155 94-95 21.815840502962633 29.473311560958816 26.390769450954956 22.320078485123602 96-97 21.82755406375112 29.398190012767678 26.32516483446195 22.449091089019255 98-99 21.790236265503008 29.376070806908288 26.27864973508982 22.555043192498882 100 21.74319684835145 29.368973027332075 26.202689780572236 22.68514034374424 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 48048.0 1 32208.5 2 12514.5 3 7985.5 4 7129.0 5 7233.0 6 7563.5 7 7520.0 8 7571.5 9 7938.5 10 8979.0 11 15978.5 12 106784.5 13 126739.0 14 61784.0 15 65995.5 16 75793.0 17 85819.0 18 95550.0 19 106161.5 20 121458.0 21 147081.5 22 184482.0 23 226752.5 24 265198.0 25 307268.5 26 355915.0 27 414662.5 28 496023.5 29 576053.0 30 632500.0 31 693218.5 32 763164.0 33 812992.5 34 856033.0 35 921971.5 36 984889.5 37 1022882.5 38 1073817.0 39 1119847.0 40 1145437.5 41 1198573.0 42 1268179.5 43 1273163.5 44 1231599.5 45 1218374.0 46 1214597.0 47 1200692.5 48 1178011.0 49 1157051.0 50 1141546.0 51 1123212.0 52 1099176.5 53 1048122.0 54 978013.0 55 903846.5 56 824954.0 57 744476.5 58 655165.0 59 551165.0 60 446245.5 61 357734.5 62 277159.0 63 202912.5 64 146275.5 65 104134.5 66 72978.5 67 53880.5 68 39912.5 69 29015.0 70 21630.0 71 17024.0 72 14101.5 73 11283.5 74 9286.0 75 8516.0 76 6295.0 77 3095.5 78 1389.5 79 870.0 80 540.5 81 374.0 82 265.5 83 183.0 84 129.0 85 86.5 86 61.5 87 44.5 88 30.0 89 19.5 90 10.5 91 8.0 92 6.0 93 3.5 94 2.5 95 1.5 96 1.0 97 1.0 98 1.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3272129838201295 2 0.006380422919885835 3 2.398436143270471 4 8.038328088045147E-4 5 3.628411984187046E-5 6 0.00862445617779844 7 0.00758896321923429 8 2.958551310183283E-4 9 2.791086141682343E-6 10-11 7.256823968374091E-5 12-13 0.0 14-15 0.0 16-17 3.070194755850577E-5 18-19 0.0 20-21 3.139971909392636E-4 22-23 3.90752059835528E-5 24-25 5.303063669196451E-4 26-27 0.0035069997370238636 28-29 0.006165509286976295 30-31 0.005746846365723944 32-33 0.015461221681849338 34-35 0.009950222095097551 36-37 0.005125829699199623 38-39 0.005243055317150281 40-41 0.008409542544888899 42-43 0.01909800692446143 44-45 0.034506197969618804 46-47 0.033493033700188114 48-49 0.04027397748140537 50-51 0.04036887441022256 52-53 0.06254684489203045 54-55 0.03341767437436269 56-57 0.02234962227952136 58-59 0.016993527973632943 60-61 0.017959243778655036 62-63 0.02501371400175716 64-65 0.04500905512076946 66-67 0.04814204931480789 68-69 0.050750319314210035 70-71 0.06126852743913994 72-73 0.06896773856097069 74-75 0.059616204443264 76-77 0.0601451152671128 78-79 0.054458277253435036 80-81 0.05217098216032635 82-83 0.048576063209839496 84-85 0.05048516613075022 86-87 0.05826810983683143 88-89 0.06506580013489878 90-91 0.061190377027172844 92-93 0.06541468590260907 94-95 0.07369025631269721 96-97 0.0731683232042026 98-99 0.04786991841599386 100 0.007044701421606233 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3.5828346E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.125551486476255 #Duplication Level Percentage of deduplicated Percentage of total 1 72.48409573084912 29.084643151988598 2 14.126783559540268 11.336899621132785 3 4.766481347301337 5.7377307813140535 4 2.4065233424309924 3.862523051204868 5 1.396924784902841 2.8026188689676856 6 0.9145504991222982 2.20181058837086 7 0.6631390381214433 1.8626173731784015 8 0.4952915945379592 1.589907870196144 9 0.36775185568191043 1.3280621417470515 >10 2.03613966321328 15.233539616356682 >50 0.17345929899395546 4.749884006598527 >100 0.16042093184151968 13.82180971600257 >500 0.007993360846992772 1.8544671993074153 >1k 3.959404367369787E-4 0.3056547842075897 >5k 1.4014908427269613E-5 0.034652372386452385 >10k+ 3.503727094453222E-5 4.193178857040344 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 1348672 3.764259728875009 No Hit AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAGGTATATCTCGTA 53697 0.14987295254991678 TruSeq Adapter, Index 5 (97% over 37bp) AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 38142 0.10645760761604792 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.17204813194558297 5.582172283364686E-6 0.0 2.791086141682343E-6 2.7910861416823427E-5 2 0.18395769651214153 5.582172283364686E-6 0.0 8.373258425047028E-6 2.7910861416823427E-5 3 0.19745259800717566 5.582172283364686E-6 0.0 1.6746516850094057E-5 3.628411984187046E-5 4 0.20051721059074287 8.373258425047028E-6 0.0 1.6746516850094057E-5 6.140389511701154E-5 5 0.20094703785656196 1.1164344566729372E-5 0.0 1.6746516850094057E-5 6.140389511701154E-5 6 0.2011145030250629 1.6746516850094057E-5 0.0 1.95376029917764E-5 6.419498125869389E-5 7 0.20140198489765618 1.6746516850094057E-5 0.0 1.95376029917764E-5 6.419498125869389E-5 8 0.20231187897984462 2.2328689133458743E-5 0.0 1.95376029917764E-5 6.698606740037623E-5 9 0.2032664304403 2.2328689133458743E-5 0.0 2.2328689133458743E-5 6.698606740037623E-5 10-11 0.20359996523423102 2.5119775275141083E-5 0.0 2.7910861416823427E-5 7.535932582542325E-5 12-13 0.2037353329121026 2.7910861416823427E-5 0.0 2.7910861416823427E-5 8.652367039215262E-5 14-15 0.20380371452257384 3.070194755850577E-5 0.0 2.7910861416823427E-5 8.931475653383497E-5 16-17 0.2038609317884783 4.0470749054393975E-5 0.0 3.070194755850577E-5 9.071029960467614E-5 18-19 0.2039446643727288 5.023955055028217E-5 0.0 3.628411984187046E-5 9.489692881719966E-5 20-21 0.20404235238768767 6.83816104712174E-5 0.0 4.605292133775866E-5 1.0327018724224668E-4 22-23 0.20417074235020505 7.396378275458208E-5 0.0 4.744846440859983E-5 1.0885235952561137E-4 24-25 0.2044665974812234 7.81504119671056E-5 0.0 4.744846440859983E-5 1.1443453180897606E-4 26-27 0.20544487317388305 8.373258425047029E-5 0.0 4.744846440859983E-5 1.2141224716318191E-4 28-29 0.2084494774054041 9.7688014958882E-5 0.0 4.744846440859983E-5 1.2838996251738778E-4 30-31 0.2161947414485726 1.0606127338392902E-4 0.0 5.023955055028217E-5 1.3536767787159364E-4 32-33 0.23156804391695895 1.116434456672937E-4 0.0 5.303063669196451E-5 1.4932310858000535E-4 34-35 0.25931981342370647 1.1862116102149956E-4 0.0 6.000835204617037E-5 1.5769636700505235E-4 36-37 0.3032291806046531 1.2559887637570542E-4 0.0 6.419498125869389E-5 1.618829962175759E-4 38-39 0.36700968557130714 1.2559887637570542E-4 0.0 6.977715354205858E-5 1.702562546426229E-4 40-41 0.4531146930422074 1.2978550558822893E-4 0.0 7.256823968374091E-5 1.7723396999682877E-4 42-43 0.5634421415936979 1.3397213480075245E-4 0.0 7.256823968374091E-5 1.8560722842187577E-4 44-45 0.7018521033597253 1.367632209424348E-4 2.791086141682343E-6 7.256823968374091E-5 1.911894007052405E-4 46-47 0.8749385193500141 1.4374093629664064E-4 2.791086141682343E-6 7.535932582542325E-5 1.9816711605944636E-4 48-49 1.0833321750325844 1.4792756550916416E-4 2.791086141682343E-6 7.535932582542325E-5 2.093314606261757E-4 50-51 1.3284844352011116 1.563008239342112E-4 2.791086141682343E-6 8.094149810878794E-5 2.1212254676785804E-4 52-53 1.606570116298419 1.6048745314673472E-4 2.791086141682343E-6 8.373258425047029E-5 2.3026460668879327E-4 54-55 1.9224471037541058 1.7304734078430525E-4 2.791086141682343E-6 8.79192134629938E-5 2.4282449432636382E-4 56-57 2.2826702633719123 1.8421168535103465E-4 2.791086141682343E-6 9.071029960467614E-5 2.4282449432636382E-4 58-59 2.687394500432702 1.995626591302875E-4 2.791086141682343E-6 9.629247188804083E-5 2.4701112353888734E-4 60-61 3.137115232726624 2.0654037448449337E-4 2.791086141682343E-6 1.0187464417140552E-4 2.539888388930932E-4 62-63 3.619843349732081 2.3026460668879327E-4 2.791086141682343E-6 1.0606127338392902E-4 2.581754681056167E-4 64-65 4.1402608984517455 2.44220037397205E-4 2.791086141682343E-6 1.0885235952561137E-4 2.651531834598226E-4 66-67 4.7027024356636495 2.498022096805697E-4 2.791086141682343E-6 1.1862116102149956E-4 2.735264418848696E-4 68-69 5.306879083952131 2.5677992503477556E-4 2.791086141682343E-6 1.2001670409234074E-4 2.777130710973931E-4 70-71 5.947631520584288 2.6096655424729905E-4 2.791086141682343E-6 1.2141224716318191E-4 2.818997003099166E-4 72-73 6.626899550428591 2.679442696015049E-4 2.791086141682343E-6 1.2559887637570542E-4 2.8887741566412247E-4 74-75 7.3376077701158735 2.721308988140284E-4 2.791086141682343E-6 1.3118104865907012E-4 2.944595879474872E-4 76-77 8.081818792304842 2.791086141682343E-4 2.791086141682343E-6 1.3397213480075245E-4 2.958551310183283E-4 78-79 8.857252299617738 2.8189970030991667E-4 2.791086141682343E-6 1.3955430708411715E-4 2.9864621716001064E-4 80-81 9.663821768384173 2.9166850180580484E-4 2.791086141682343E-6 1.4792756550916416E-4 3.126016478684224E-4 82-83 10.500130259990232 2.986462171600107E-4 2.791086141682343E-6 1.507186516508465E-4 3.126016478684224E-4 84-85 11.361907412639143 2.986462171600107E-4 4.186629212523514E-6 1.563008239342112E-4 3.1957936322262826E-4 86-87 12.253134710712015 3.0562393251421655E-4 5.582172283364686E-6 1.563008239342112E-4 3.2097490629346944E-4 88 12.932313425799784 3.0701947558505773E-4 5.582172283364686E-6 1.563008239342112E-4 3.293481647185165E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 181040 0.0 84.93342 8 TGGTATC 183485 0.0 83.73774 7 AGTGGTA 184135 0.0 82.89053 5 GTATCAA 185435 0.0 82.819046 9 GTGGTAT 184445 0.0 82.72831 6 GCAGTGG 189220 0.0 80.63758 3 AAGCAGT 184675 0.0 80.3372 1 CAGTGGT 196190 0.0 77.65424 4 AGCAGTG 201990 0.0 75.22295 2 CAACGCA 176015 0.0 44.52699 12-13 ATCAACG 177945 0.0 44.110027 10-11 ACGCAGA 179665 0.0 43.695545 14-15 TCAACGC 175515 0.0 43.695076 12-13 AACGCAG 177915 0.0 43.20854 14-15 AGAGTAC 183000 0.0 42.77394 18-19 CGCAGAG 180670 0.0 42.552383 16-17 GAGTACT 180070 0.0 42.153645 20-21 TATCAAC 184580 0.0 41.582237 10-11 AGTACTT 190965 0.0 40.648342 20-21 GTACTTT 191755 0.0 39.59186 22-23 >>END_MODULE