FastQCFastQC Report
Thu 26 May 2016
SRR1660732_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660732_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22899761
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT13366745.837065286401898No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA367210.16035538536843247No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT327500.14301459303439892No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGGTA1720550.085.2012565
GGTATCA1719200.085.115448
TGGTATC1736700.084.045927
GTGGTAT1746350.083.950746
GTATCAA1741500.083.840249
AAGCAGT1769350.083.097451
GCAGTGG1793650.082.185433
CAGTGGT1813550.080.709684
AGCAGTG1850550.079.670652
CAACGCA1701000.044.28981412-13
ATCAACG1710550.044.1282810-11
ACGCAGA1719450.043.8375214-15
AGAGTAC1709150.043.45635618-19
GAGTACT1658850.043.24670820-21
TCAACGC1697750.043.16327312-13
AACGCAG1712650.042.8394714-15
CGCAGAG1732700.042.30687716-17
AGTACTT1740950.042.30340220-21
TATCAAC1745550.041.79479210-11
GTACTTT1744800.041.04303422-23
Produced by FastQC (version 0.11.5)