##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660732_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 22899761 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.85934578094505 34.0 31.0 34.0 30.0 34.0 2 31.983036591517266 34.0 31.0 34.0 30.0 34.0 3 32.04470544474241 34.0 31.0 34.0 30.0 34.0 4 35.45848120423615 37.0 35.0 37.0 33.0 37.0 5 35.42550968108357 37.0 35.0 37.0 33.0 37.0 6 35.50395019406535 37.0 35.0 37.0 33.0 37.0 7 35.451005886043966 37.0 35.0 37.0 33.0 37.0 8 35.46318666819274 37.0 35.0 37.0 33.0 37.0 9 37.142810529769285 39.0 37.0 39.0 33.0 39.0 10-11 37.07529812210704 39.0 37.0 39.0 33.0 39.0 12-13 37.10535946204854 39.0 37.0 39.0 33.0 39.0 14-15 38.479192512096525 40.0 38.0 41.0 34.0 41.0 16-17 38.458032946282714 40.0 38.0 41.0 34.0 41.0 18-19 38.4185915521127 40.0 38.0 41.0 33.5 41.0 20-21 38.290655326053404 40.0 38.0 41.0 33.5 41.0 22-23 38.10646148228359 40.0 38.0 41.0 33.0 41.0 24-25 38.09492627455806 40.0 38.0 41.0 33.0 41.0 26-27 38.084867959975654 40.0 38.0 41.0 33.0 41.0 28-29 38.00296924496286 40.0 38.0 41.0 33.0 41.0 30-31 37.909065142644934 40.0 38.0 41.0 32.5 41.0 32-33 37.77808836083486 40.0 38.0 41.0 32.0 41.0 34-35 37.615327972200234 40.0 37.5 41.0 31.5 41.0 36-37 37.414593693794444 40.0 37.0 41.0 31.0 41.0 38-39 36.969296142435724 39.5 35.0 41.0 30.5 41.0 40-41 36.901181261236744 39.0 35.0 41.0 30.5 41.0 42-43 36.9009792285605 40.0 35.0 41.0 30.0 41.0 44-45 36.895884415562236 40.0 35.0 41.0 30.0 41.0 46-47 36.98263671834828 40.0 35.0 41.0 31.0 41.0 48-49 36.86876042068736 40.0 35.0 41.0 30.0 41.0 50-51 35.489786814805626 38.5 34.0 40.0 28.0 40.5 52-53 35.63508654085953 38.5 34.5 39.5 28.0 40.5 54-55 35.992774247731234 39.0 35.0 41.0 27.0 41.0 56-57 35.59854626867066 39.0 35.0 41.0 24.0 41.0 58-59 35.39917353285914 39.0 35.0 41.0 23.5 41.0 60-61 35.17140128667718 39.0 35.0 41.0 23.0 41.0 62-63 34.941425262036574 38.5 34.5 41.0 23.0 41.0 64-65 34.68483225654626 37.5 34.0 40.0 23.0 41.0 66-67 34.413870018119404 37.0 34.0 40.0 23.0 41.0 68-69 34.12886630126838 36.5 34.0 40.0 22.0 41.0 70-71 33.819976570934514 36.0 33.5 39.0 22.5 41.0 72-73 33.47278039277353 35.5 33.0 39.0 22.0 41.0 74-75 33.09058587991377 35.0 33.0 38.5 20.5 40.0 76-77 32.695320706622226 35.0 33.0 37.0 20.0 39.5 78-79 32.32525865662964 35.0 32.5 37.0 19.5 39.0 80-81 32.00443026457787 35.0 32.0 36.0 19.0 39.0 82-83 31.692216241907502 35.0 32.0 36.0 18.5 37.5 84-85 31.40233107673045 35.0 32.0 35.5 18.0 37.0 86-87 31.18215797099367 35.0 31.5 35.0 17.0 37.0 88-89 30.972396786149865 35.0 31.0 35.0 17.0 36.0 90-91 30.79586937610397 35.0 31.0 35.0 15.5 36.0 92-93 30.64177702553315 35.0 31.0 35.0 15.0 36.0 94-95 30.458667974744365 35.0 31.0 35.0 15.0 35.5 96-97 30.316424394123587 34.5 30.5 35.0 12.5 35.0 98-99 30.207240962034497 34.0 30.0 35.0 11.5 35.0 100 30.122171930091323 34.0 30.0 35.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5766216132590003 1101 2 0.576835695793747 1101 3 0.5513650530465597 1101 4 0.4979484120488493 1101 5 0.507428322241978 1101 6 0.4512449305538908 1101 7 0.500351740862186 1101 8 0.48213101795446534 1101 9 0.5791184443786577 1101 10-11 0.6106729979847572 1101 12-13 0.5751101697306638 1101 14-15 0.7100169459810246 1101 16-17 0.6704594790169054 1101 18-19 0.6834394820564071 1101 20-21 0.7506758856698283 1101 22-23 0.7875031816304414 1101 24-25 0.7723794451142965 1101 26-27 0.7294043258604788 1101 28-29 0.7280014227158915 1101 30-31 0.6663781961983659 1101 32-33 0.6657421681773741 1101 34-35 0.6924216050046041 1101 36-37 0.7004063604908595 1101 38-39 0.7162227112283546 1101 40-41 0.7138371647964661 1101 42-43 0.7130969592713541 1101 44-45 0.7349949446402135 1101 46-47 0.7463128578454103 1101 48-49 0.7982885822351378 1101 50-51 0.7349002344291833 1101 52-53 0.7576972367915502 1101 54-55 0.8382646207589559 1101 56-57 0.9010215014964587 1101 58-59 0.7566750393958657 1101 60-61 0.7621117934320623 1101 62-63 0.7154696175589379 1101 64-65 0.7092685065309539 1101 66-67 0.6500641235428333 1101 68-69 0.6446471159387812 1101 70-71 0.6451701533420149 1101 72-73 0.634529803894381 1101 74-75 0.6137134995672824 1101 76-77 0.6186874668193454 1101 78-79 0.6081489776758318 1101 80-81 0.5779368221114964 1101 82-83 0.5781900613078044 1101 84-85 0.5368863600098344 1101 86-87 0.49520769479224214 1101 88-89 0.5325112038990092 1101 90-91 0.5298228101778868 1101 92-93 0.5287343415104644 1101 94-95 0.5296067567855225 1101 96-97 0.5358306821417393 1101 98-99 0.4624813124534235 1101 100 0.46170470105489514 1102 1 0.44944548388650674 1102 2 0.501513998527436 1102 3 0.5106485800854941 1102 4 0.4361174242817185 1102 5 0.43165632699115264 1102 6 0.39151841542334154 1102 7 0.41547309163981083 1102 8 0.4477302155849543 1102 9 0.4788964366260444 1102 10-11 0.5186733622994311 1102 12-13 0.5503608336222214 1102 14-15 0.6279477634525605 1102 16-17 0.6273503108259817 1102 18-19 0.5729134245945247 1102 20-21 0.5832002469934139 1102 22-23 0.6116242546130692 1102 24-25 0.5982182803547929 1102 26-27 0.55948882121411 1102 28-29 0.5945860932542573 1102 30-31 0.5529974128043165 1102 32-33 0.5569961077781755 1102 34-35 0.5669000838360247 1102 36-37 0.5804682870119962 1102 38-39 0.5774668735941333 1102 40-41 0.586085420094399 1102 42-43 0.5753037170116855 1102 44-45 0.5838571311446756 1102 46-47 0.5876337360652144 1102 48-49 0.6132853060729602 1102 50-51 0.5656088045891039 1102 52-53 0.5907735751843077 1102 54-55 0.6617413477786656 1102 56-57 0.6831151688288486 1102 58-59 0.5757700678605815 1102 60-61 0.5858257701594525 1102 62-63 0.56158813892052 1102 64-65 0.5789282829233215 1102 66-67 0.5436899449045995 1102 68-69 0.5186173721716898 1102 70-71 0.5231549090746697 1102 72-73 0.5344790804128365 1102 74-75 0.49883639164411164 1102 76-77 0.47540136239059194 1102 78-79 0.4218975280631696 1102 80-81 0.38638452314719984 1102 82-83 0.4124901974055426 1102 84-85 0.39875522888632275 1102 86-87 0.31207488351374835 1102 88-89 0.31442181550593773 1102 90-91 0.32454330439053436 1102 92-93 0.3381269934030158 1102 94-95 0.3941055005757157 1102 96-97 0.3225233722297638 1102 98-99 0.27147064788820785 1102 100 0.2582443326768171 1103 1 0.37482731218943854 1103 2 0.4307972597205918 1103 3 0.42140645002676536 1103 4 0.34282017271652165 1103 5 0.40309156014732395 1103 6 0.36950785351028514 1103 7 0.34827547773320333 1103 8 0.39406725870067305 1103 9 0.4351398150091441 1103 10-11 0.4349282845766922 1103 12-13 0.45131523497765613 1103 14-15 0.5308236951608976 1103 16-17 0.47891558784237276 1103 18-19 0.4755598670090322 1103 20-21 0.5169525502504371 1103 22-23 0.5575144939188377 1103 24-25 0.6039952595678244 1103 26-27 0.5330846554480715 1103 28-29 0.5373050594285473 1103 30-31 0.5262841983555688 1103 32-33 0.47013487101149565 1103 34-35 0.5003423535951654 1103 36-37 0.5180234595729303 1103 38-39 0.5217864708292268 1103 40-41 0.48901876105676223 1103 42-43 0.5135627383523627 1103 44-45 0.5175676700311058 1103 46-47 0.5978195810309828 1103 48-49 0.6351566301648077 1103 50-51 0.5502229672129815 1103 52-53 0.518796840578581 1103 54-55 0.6259391850906013 1103 56-57 0.6164906448659337 1103 58-59 0.5639627958200322 1103 60-61 0.5424069456218277 1103 62-63 0.5188042685015404 1103 64-65 0.46647462524832406 1103 66-67 0.4478166315626879 1103 68-69 0.4428495696306811 1103 70-71 0.45852042030308837 1103 72-73 0.4440507992804257 1103 74-75 0.40671638404636923 1103 76-77 0.383353420011737 1103 78-79 0.38444894040823385 1103 80-81 0.3289989580287056 1103 82-83 0.33931943046905744 1103 84-85 0.33355823830207143 1103 86-87 0.2869639072894934 1103 88-89 0.30026000355410787 1103 90-91 0.34477103896255556 1103 92-93 0.2843615186258859 1103 94-95 0.3327933733662185 1103 96-97 0.2952123804355793 1103 98-99 0.27972305717974066 1103 100 0.3009648658014825 1104 1 0.3739490910847607 1104 2 0.4002596429458265 1104 3 0.3691039144450059 1104 4 0.30606281757327736 1104 5 0.3172028463922487 1104 6 0.2635094379715497 1104 7 0.30347192156350644 1104 8 0.31697104012609145 1104 9 0.3893317022073788 1104 10-11 0.34677511589594445 1104 12-13 0.34054730732624705 1104 14-15 0.4226475351605288 1104 16-17 0.42127377411927824 1104 18-19 0.3922991502638382 1104 20-21 0.3448191865235941 1104 22-23 0.41070787624321525 1104 24-25 0.4220281308455114 1104 26-27 0.38768291222408635 1104 28-29 0.3821743993310065 1104 30-31 0.3491517154438384 1104 32-33 0.35429669054823876 1104 34-35 0.3558930046413451 1104 36-37 0.3758343068717238 1104 38-39 0.3489962093380683 1104 40-41 0.3739631283614173 1104 42-43 0.359418064468187 1104 44-45 0.38896305882470017 1104 46-47 0.41588447022794384 1104 48-49 0.4321154527640516 1104 50-51 0.3941535084292056 1104 52-53 0.3724163531832474 1104 54-55 0.4819898497987438 1104 56-57 0.48568681583478934 1104 58-59 0.4215806317841029 1104 60-61 0.4392701056075836 1104 62-63 0.39218897079340564 1104 64-65 0.4005795006073569 1104 66-67 0.42254685930584657 1104 68-69 0.3880291204181816 1104 70-71 0.38066884002211765 1104 72-73 0.37642250832508495 1104 74-75 0.3464191510844614 1104 76-77 0.35348878081114066 1104 78-79 0.38313128705289756 1104 80-81 0.35073250741940143 1104 82-83 0.3529496888700798 1104 84-85 0.3092517840160056 1104 86-87 0.2744685724896492 1104 88-89 0.26168757029912015 1104 90-91 0.2637530114429616 1104 92-93 0.26708055743582193 1104 94-95 0.3313012101595092 1104 96-97 0.27903337898115055 1104 98-99 0.2872955650840616 1104 100 0.2655460335540418 1105 1 0.3126368084829494 1105 2 0.36721127576465307 1105 3 0.3351276556487335 1105 4 0.3156649339160893 1105 5 0.32401429037562224 1105 6 0.25859890587448575 1105 7 0.2923674873159712 1105 8 0.30446199356752857 1105 9 0.28776917547256176 1105 10-11 0.3101319309987005 1105 12-13 0.3032801412310704 1105 14-15 0.3633995180495333 1105 16-17 0.31043477623334326 1105 18-19 0.34825149091013685 1105 20-21 0.38042256442025746 1105 22-23 0.43433345342661767 1105 24-25 0.4347825211059444 1105 26-27 0.4493556588455263 1105 28-29 0.4013241623281587 1105 30-31 0.40815738138177693 1105 32-33 0.37651977119513447 1105 34-35 0.4027780509564636 1105 36-37 0.3786799929085092 1105 38-39 0.3611925850165676 1105 40-41 0.38696570027000377 1105 42-43 0.4272522780510428 1105 44-45 0.4355074855038765 1105 46-47 0.43976000734851084 1105 48-49 0.4620474858738248 1105 50-51 0.3671932310725694 1105 52-53 0.40027081844523593 1105 54-55 0.515933976670901 1105 56-57 0.5332434288040204 1105 58-59 0.4559622531523573 1105 60-61 0.4554411766984501 1105 62-63 0.45934447388562916 1105 64-65 0.49108908913459715 1105 66-67 0.4156192259072782 1105 68-69 0.4318220572770386 1105 70-71 0.42931092437677165 1105 72-73 0.38228674849834476 1105 74-75 0.40629671790421895 1105 76-77 0.4217926845352409 1105 78-79 0.4466104413591552 1105 80-81 0.4100763232129978 1105 82-83 0.39285317372698714 1105 84-85 0.36990314845739647 1105 86-87 0.3382838635387273 1105 88-89 0.3171367821267381 1105 90-91 0.34762668620995285 1105 92-93 0.3060654740097135 1105 94-95 0.35966746510943537 1105 96-97 0.3861149985194281 1105 98-99 0.32628348598695567 1105 100 0.29816165227653 1106 1 0.19801597521973946 1106 2 0.25552984184110983 1106 3 0.24593891282446378 1106 4 0.226213190483044 1106 5 0.2291240054680017 1106 6 0.21937667635747005 1106 7 0.22509701281490635 1106 8 0.2427949496882249 1106 9 0.23474274830264363 1106 10-11 0.22784697267869802 1106 12-13 0.2463788201918362 1106 14-15 0.3024444458593578 1106 16-17 0.2708704510967408 1106 18-19 0.25251353997284554 1106 20-21 0.2752099360272382 1106 22-23 0.2561952065405393 1106 24-25 0.25295198818361797 1106 26-27 0.29760349361905725 1106 28-29 0.27250139861754263 1106 30-31 0.244621074001941 1106 32-33 0.25562976808663507 1106 34-35 0.2550707225110287 1106 36-37 0.2256305226208326 1106 38-39 0.26439255789404825 1106 40-41 0.2754594035866731 1106 42-43 0.2733971877906498 1106 44-45 0.329017126397666 1106 46-47 0.32563214386297545 1106 48-49 0.32748543920266115 1106 50-51 0.21200709168453358 1106 52-53 0.26663317918552565 1106 54-55 0.42605240827580104 1106 56-57 0.4416897889601046 1106 58-59 0.3799497118139712 1106 60-61 0.4114436169584508 1106 62-63 0.3559097496137653 1106 64-65 0.3411740566677466 1106 66-67 0.32250673506686667 1106 68-69 0.3039177482578168 1106 70-71 0.32227187223617904 1106 72-73 0.28509516794715495 1106 74-75 0.29339133913881454 1106 76-77 0.25316209097297104 1106 78-79 0.3014189047946729 1106 80-81 0.2563921598120231 1106 82-83 0.24259548011579923 1106 84-85 0.2200539554934693 1106 86-87 0.20335093178855246 1106 88-89 0.24858636384741573 1106 90-91 0.20362223513135547 1106 92-93 0.16797829908636075 1106 94-95 0.208271972747486 1106 96-97 0.2619262023680271 1106 98-99 0.24823953615793926 1106 100 0.19706271629947025 1107 1 0.20100543485407485 1107 2 0.2042256427275646 1107 3 0.2131603390168877 1107 4 0.184395104764441 1107 5 0.2141959030673135 1107 6 0.16787799631622846 1107 7 0.18314150630214954 1107 8 0.19675510424967513 1107 9 0.20731526327537608 1107 10-11 0.22691894669086565 1107 12-13 0.1962849828524611 1107 14-15 0.2428079782680257 1107 16-17 0.23346580312128395 1107 18-19 0.23708789687034937 1107 20-21 0.259495170205966 1107 22-23 0.26406081153913874 1107 24-25 0.2596212117243013 1107 26-27 0.25441286399482976 1107 28-29 0.2780555490766474 1107 30-31 0.2033749779921692 1107 32-33 0.21724198374892723 1107 34-35 0.24192429249766434 1107 36-37 0.24113449312119428 1107 38-39 0.23392818720283515 1107 40-41 0.2403910761190673 1107 42-43 0.2805082058239634 1107 44-45 0.30025558257106155 1107 46-47 0.26776061775374416 1107 48-49 0.31304235397351476 1107 50-51 0.23625965856455622 1107 52-53 0.26145343580095215 1107 54-55 0.35845889822029875 1107 56-57 0.38416415394357983 1107 58-59 0.31081537693921035 1107 60-61 0.32001748574412403 1107 62-63 0.3268177065236344 1107 64-65 0.27492340246721625 1107 66-67 0.32228029036346584 1107 68-69 0.3202936957104754 1107 70-71 0.3135475884876442 1107 72-73 0.3091881392956779 1107 74-75 0.2896422440916524 1107 76-77 0.29110584533570005 1107 78-79 0.3401961801793689 1107 80-81 0.2668339258128114 1107 82-83 0.2916442938467263 1107 84-85 0.3088673682249663 1107 86-87 0.26247577775506414 1107 88-89 0.2758489362755867 1107 90-91 0.29390312632524385 1107 92-93 0.24898811881782734 1107 94-95 0.28814914440137684 1107 96-97 0.3123040948633644 1107 98-99 0.3095252937292834 1107 100 0.29181102378479906 1108 1 0.17183142644636007 1108 2 0.215286538386934 1108 3 0.22292930419230572 1108 4 0.21199001545866025 1108 5 0.22463836588623565 1108 6 0.1328383000475739 1108 7 0.13937406667122332 1108 8 0.17210232013097482 1108 9 0.19543006724663314 1108 10-11 0.17003787540307513 1108 12-13 0.16983350266011144 1108 14-15 0.19620236875064023 1108 16-17 0.201509819594186 1108 18-19 0.19544383460318926 1108 20-21 0.18950379851435173 1108 22-23 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-1.1790176556902274 2316 92-93 -1.042701764565468 2316 94-95 -1.2583589901298602 2316 96-97 -1.3937920539422173 2316 98-99 -1.3093746780113769 2316 100 -1.0971777297136 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 11832.0 3 2971.0 4 2212.0 5 1698.0 6 2719.0 7 12582.0 8 24483.0 9 28932.0 10 30147.0 11 28961.0 12 29529.0 13 32346.0 14 38199.0 15 47512.0 16 61451.0 17 77186.0 18 84417.0 19 94229.0 20 108960.0 21 132538.0 22 173738.0 23 258308.0 24 374640.0 25 436870.0 26 423430.0 27 362787.0 28 329948.0 29 325309.0 30 363369.0 31 437723.0 32 542745.0 33 700125.0 34 960837.0 35 1467568.0 36 2524889.0 37 4698495.0 38 5903683.0 39 1758512.0 40 3881.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 15.644978855470368 33.372210148243 27.500564408045026 23.48224658824161 2 20.703527461173692 33.92368305703687 27.11750490064589 18.25528458114355 3 25.78530357944598 25.262359844080095 27.033111633768087 21.91922494270584 4 18.73949861788261 27.77770948618235 24.842906607150613 28.639885288784427 5 21.43053566815111 32.938463626849526 26.209305991618827 19.42169471338054 6 28.392255465429184 27.297945666561173 25.601450745130816 18.708348122878828 7 20.78593172839545 25.981746708911817 32.80692189369937 20.425399668993368 8 27.167174396992433 26.5587201469918 25.784740067466284 20.489365388549484 9 26.475217706827824 25.068088316550373 27.584771725074848 20.871922251546955 10-11 20.403036641467576 29.939413478359576 29.265753046576048 20.3917968335968 12-13 20.301512745372513 26.64487678997708 29.166928026395343 23.886682438255065 14-15 20.28729293026514 33.39651967977081 25.87754196570511 20.438645424258937 16-17 23.764168810321205 26.437908143022838 25.952947507221953 23.844975539434003 18-19 20.318830798620233 29.903630729324405 25.955977072632102 23.821561399423263 20-21 23.707444413013818 30.05070640854334 25.919075792085668 20.322773386357174 22-23 23.69497632737337 26.65435735833306 29.279705919417736 20.37096039487584 24-25 20.37905164923261 29.87003146692629 25.817629337695315 23.933287546145788 26-27 20.421729306200028 26.53649982556463 32.68628635530776 20.35548451292758 28-29 20.396779456220425 26.646309419304835 32.665457092254655 20.29145403222008 30-31 20.328796199494704 26.558828867627703 32.679968487657604 20.432406445219982 32-33 20.448049518789297 26.7114932862362 32.45736921945868 20.383087975515828 34-35 20.47521482023509 26.827869481482235 32.33142431313512 20.365491385147553 36-37 20.391981737907923 26.784566360552876 32.4260650956402 20.397386805899004 38-39 20.4430719915107 26.760707785133338 32.43371019327155 20.362510030084408 40-41 20.47124767459437 26.789509951936154 32.41523214015476 20.324010233314716 42-43 20.46049811307959 26.836091394978318 32.357363549287115 20.346046942654972 44-45 20.502370469708918 26.918666856312985 32.316243534710196 20.262719139267908 46-47 20.483590836653022 27.021257960464617 32.2614509167381 20.23370028614426 48-49 20.56590560701783 27.02047892315718 32.13498884232963 20.278626627495353 50-51 20.59920308262147 27.0738333163149 32.06992505011113 20.257038550952498 52-53 20.741716034828077 27.184471473070516 31.79327809065178 20.28053440144963 54-55 21.091460721615356 27.47262037942469 30.99624149470803 20.439677404251928 56-57 21.586278574918005 28.03322597532041 29.838855877605308 20.541639572156278 58-59 21.75049706992946 28.542090317712738 29.142134841714114 20.56527777064369 60-61 21.932936255572923 28.7475430354574 28.777231630228435 20.542289078741245 62-63 22.048857656692434 28.91128480427759 28.568048363706076 20.471809175323898 64-65 22.16753313783037 28.96315221777263 28.49058046926263 20.378734175134365 66-67 22.294906214280193 28.827712114638736 28.602161609477267 20.275220061603804 68-69 22.419397336933507 28.683255541554697 28.725376175785723 20.171970945726077 70-71 22.588887903382563 28.557441662112552 28.762792565024487 20.09087786948039 72-73 22.798725582196727 28.441929124800343 28.743406654553148 20.01593863844978 74-75 23.03240189461509 28.50167153854008 28.604303408153186 19.861623158691643 76-77 23.361794975839654 28.599070624571404 28.291999572344167 19.74713482724477 78-79 23.66793484215089 28.752711726242673 27.921704415959457 19.65764901564698 80-81 23.955500956855502 28.942513413605315 27.55062980136754 19.551355828171644 82-83 24.172252004570684 29.077991039269364 27.194297451386205 19.555459504773747 84-85 24.346511438133344 29.105092051977287 27.009055665715625 19.53934084417374 86-87 24.57090607567224 29.098159150679482 26.88158243592218 19.4493523377261 88-89 24.835443975103598 29.058289369479745 26.738029029797744 19.36823762561891 90-91 25.042489491139158 29.020700183431803 26.622628223561534 19.314182101867505 92-93 25.23337278364241 29.01983277809461 26.527724261187895 19.219070177075082 94-95 25.419240879892545 28.993072184810536 26.406011532839646 19.181675402457273 96-97 25.55814820581766 28.92372759034773 26.32899524993182 19.189128953902788 98-99 25.76502981023175 28.909658104083906 26.230039474795998 19.095272610888344 100 25.84239136850358 28.919695355863677 26.13212578379654 19.105787491836203 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 36146.0 1 25543.5 2 12461.0 3 9475.5 4 8990.0 5 8931.0 6 8955.0 7 9066.5 8 9198.0 9 9360.0 10 9751.5 11 12105.0 12 102036.0 13 125236.5 14 58763.5 15 60635.0 16 68037.0 17 75360.5 18 81992.5 19 89063.0 20 98014.0 21 109980.0 22 127237.0 23 148304.5 24 169514.0 25 193921.0 26 222969.5 27 256096.0 28 295816.0 29 336733.5 30 371891.5 31 405928.5 32 442173.0 33 476809.5 34 509475.0 35 544417.5 36 582034.5 37 620240.0 38 658714.0 39 684213.5 40 694921.5 41 711837.5 42 736812.5 43 746463.5 44 746669.0 45 756767.5 46 768985.0 47 778191.0 48 777545.0 49 768281.5 50 759789.5 51 747602.5 52 728033.5 53 691195.0 54 643432.0 55 596634.0 56 546454.0 57 490972.0 58 431960.5 59 370185.0 60 305057.5 61 246588.0 62 195598.0 63 148225.0 64 109789.5 65 81065.5 66 59694.0 67 44702.5 68 33812.0 69 25889.0 70 20265.5 71 16003.0 72 12918.0 73 10351.0 74 8288.5 75 6884.5 76 5368.0 77 3805.0 78 2759.0 79 2100.5 80 1678.0 81 1382.5 82 1145.0 83 967.5 84 821.5 85 677.5 86 566.5 87 497.0 88 429.5 89 367.0 90 307.0 91 280.5 92 250.5 93 217.0 94 193.0 95 148.0 96 120.5 97 107.0 98 87.5 99 64.0 100 33.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4047203811428425 2 0.38416121460830965 3 0.16940351473537213 4 0.17585336370977844 5 0.27450504832779693 6 0.10149014218969359 7 0.13684422295935753 8 0.14251240438710255 9 0.13508001240711637 10-11 0.13167386332110628 12-13 0.13353196131610282 14-15 0.13952765707904113 16-17 0.14346656281696563 18-19 0.15489462968630982 20-21 0.13560840220122822 22-23 0.09822809941116852 24-25 0.09368656729648837 26-27 0.08832843277272633 28-29 0.09855343031746051 30-31 0.10325435274193473 32-33 0.10445087177984083 34-35 0.10508624959011581 36-37 0.10080017865688642 38-39 0.10026087171826817 40-41 0.10090498324414826 42-43 0.06312511296515277 44-45 0.06574741107560031 46-47 0.08447249733305078 48-49 0.08922800548005719 50-51 0.09873028805846489 52-53 0.10414955859146303 54-55 0.10674347212619381 56-57 0.10736356593415974 58-59 0.10680679156433118 60-61 0.10468668210118 62-63 0.10919109592453825 64-65 0.11624793813350279 66-67 0.124929251445026 68-69 0.12984851675962905 70-71 0.1283703353934567 72-73 0.11534836542617191 74-75 0.11255357643252259 76-77 0.11453176301709002 78-79 0.11334179426588775 80-81 0.10730461335382495 82-83 0.11042473325376628 84-85 0.11673702620739143 86-87 0.13037472312483958 88-89 0.13815646372903193 90-91 0.14508666706172174 92-93 0.14419364464109471 94-95 0.1341433214084636 96-97 0.13093586435247076 98-99 0.12593362873961872 100 0.1198047438137018 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2.2899761E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.337454069378985 #Duplication Level Percentage of deduplicated Percentage of total 1 63.81704421662181 25.742170899315102 2 18.259869770534078 14.731133163635205 3 7.486784253528896 9.05993447862215 4 3.466520899330678 5.5932251022917425 5 1.8105147246702875 3.6515777274161034 6 1.1054553700062757 2.67547531280259 7 0.7581354633861681 2.1406878102894837 8 0.5514742943010053 1.779605521344799 9 0.40229666424803745 1.460486089473263 >10 2.074289304144249 15.036504299127365 >50 0.1609591708203125 4.543498333640161 >100 0.10542065925067706 6.76173075323444 >500 5.223204386680762E-4 0.13677704094222054 >1k 6.697331313668458E-4 0.5259841854907695 >5k 1.0788896878454319E-5 0.04171359720537199 >10k+ 3.236669063536296E-5 6.119495685169305 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 1336674 5.837065286401898 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 36721 0.16035538536843247 No Hit AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 32750 0.14301459303439892 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.001917050575331332 1.7467431210308265E-5 0.0 1.3100573407731199E-5 6.113600923607892E-5 2 0.0019301511487390633 2.6201146815462398E-5 0.0 1.3100573407731199E-5 6.113600923607892E-5 3 0.0019432517221467944 3.056800461803946E-5 0.0 1.3100573407731199E-5 6.113600923607892E-5 4 0.0019607191533571027 5.2402293630924795E-5 0.0 1.3100573407731199E-5 6.5502867038656E-5 5 0.001978186584567411 5.676915143350186E-5 0.0 1.7467431210308265E-5 6.986972484123306E-5 6 0.001978186584567411 6.5502867038656E-5 0.0 2.183428901288533E-5 6.986972484123306E-5 7 0.001978186584567411 6.986972484123306E-5 0.0 2.183428901288533E-5 6.986972484123306E-5 8 0.001982553442369988 6.986972484123306E-5 0.0 2.6201146815462398E-5 6.986972484123306E-5 9 0.0019956540157777193 8.733715605154133E-5 0.0 4.8035435828347726E-5 6.986972484123306E-5 10-11 0.0020349557360009127 9.607087165669545E-5 0.0 4.8035435828347726E-5 7.860344044638719E-5 12-13 0.002065523740618952 1.0043772945927253E-4 0.0 4.8035435828347726E-5 7.860344044638719E-5 14-15 0.0021157426053485884 1.1353830286700372E-4 0.0 5.676915143350186E-5 7.860344044638719E-5 16-17 0.0021725117567820904 1.3100573407731197E-4 0.0 5.676915143350186E-5 8.51537271502528E-5 18-19 0.0022969672041555367 1.462897363863317E-4 0.0 6.768629593994452E-5 8.733715605154133E-5 20-21 0.0024126889359238294 1.6157373869535145E-4 0.0 7.20531537425216E-5 8.733715605154133E-5 22-23 0.0026157478237436626 1.8340802770823678E-4 0.0 7.423658264381012E-5 8.733715605154133E-5 24-25 0.0030065815970743105 1.9650860111596798E-4 0.0 8.297029824896426E-5 9.170401385411839E-5 26-27 0.004294804648834544 2.052423167211221E-4 0.0 8.952058495282985E-5 9.607087165669546E-5 28-29 0.00805248578795211 2.2489317683271892E-4 0.0 1.0698801616313813E-4 1.0698801616313813E-4 30-31 0.016973976278617055 2.445440369443157E-4 0.0 1.2227201847215784E-4 1.1353830286700372E-4 32-33 0.03572308025398169 2.62011468154624E-4 0.0 1.3537259187988904E-4 1.1353830286700372E-4 34-35 0.07035444605731911 2.8602918606879783E-4 0.0 1.6594059649792853E-4 1.2445544737344638E-4 36-37 0.1272327689358854 2.969463305752405E-4 0.0 1.724908832017941E-4 1.31005734077312E-4 38-39 0.20960917452369918 2.969463305752405E-4 0.0 1.8559145660952532E-4 1.31005734077312E-4 40-41 0.32114308965932004 3.056800461803946E-4 0.0 1.9650860111596798E-4 1.3318916297860053E-4 42-43 0.461410929135898 3.231474773907029E-4 0.0 2.0960917452369918E-4 1.3973944968246612E-4 44-45 0.6392009942811194 3.2751433519328E-4 0.0 2.183428901288533E-4 1.441063074850432E-4 46-47 0.865135666699753 3.449817664035882E-4 0.0 2.2489317683271892E-4 1.5502345199148586E-4 48-49 1.1437673956509853 3.6026576871260797E-4 0.0 2.314434635365845E-4 1.7030745430050557E-4 50-51 1.4655786145540994 3.6463262651518504E-4 0.0 2.5109432364818127E-4 1.7467431210308265E-4 52-53 1.8185910324566268 3.6681605541647355E-4 0.0 2.598280392533354E-4 1.7904116990565973E-4 54-55 2.2132916583714564 3.7118291321905063E-4 0.0 2.707451837597781E-4 1.7904116990565973E-4 56-57 2.6638422121523453 3.9083377333064745E-4 0.0 2.7729547046364373E-4 1.8340802770823678E-4 58-59 3.1791226991408337 4.1703492014610985E-4 0.0 2.860291860687979E-4 1.8340802770823678E-4 60-61 3.7422180956386404 4.3231892245512954E-4 0.0 3.2969776409456854E-4 1.8340802770823678E-4 62-63 4.337422997558796 4.3450235135641805E-4 0.0 3.449817664035882E-4 1.8340802770823678E-4 64-65 4.960385831101032 4.4978635366543784E-4 0.0 3.6681605541647355E-4 1.8559145660952532E-4 66-67 5.633078441299016 4.563366403693034E-4 0.0 3.777331999229162E-4 1.8995831441210236E-4 68-69 6.345701599243766 4.62886927073169E-4 0.0 3.908337733306474E-4 1.9650860111596798E-4 70-71 7.0999496457626785 4.6943721377703466E-4 0.0 4.061177756396672E-4 2.0087545891854506E-4 72-73 7.8819206890412525 4.890880738886315E-4 0.0 4.1703492014610985E-4 2.052423167211221E-4 74-75 8.690798126670405 5.087389340002282E-4 0.0 4.2576863575126395E-4 2.052423167211221E-4 76-77 9.525616883075767 5.109223629015167E-4 0.0 4.3231892245512954E-4 2.052423167211221E-4 78-79 10.387217578384334 5.218395074079595E-4 0.0 4.388692091589952E-4 2.183428901288533E-4 80-81 11.273969191206842 5.480406542234218E-4 0.0 4.5196978256672635E-4 2.183428901288533E-4 82-83 12.177954608347221 5.589577987298645E-4 0.0 4.7162064267832317E-4 2.183428901288533E-4 84-85 13.094874658298835 5.676915143350185E-4 0.0 4.8690464498734286E-4 2.183428901288533E-4 86-87 14.032292738775745 6.004429478543465E-4 0.0 5.043720761976512E-4 2.183428901288533E-4 88 14.742765219252725 6.244606657685205E-4 0.0 5.152892207040938E-4 2.183428901288533E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGTGGTA 172055 0.0 85.201256 5 GGTATCA 171920 0.0 85.11544 8 TGGTATC 173670 0.0 84.04592 7 GTGGTAT 174635 0.0 83.95074 6 GTATCAA 174150 0.0 83.84024 9 AAGCAGT 176935 0.0 83.09745 1 GCAGTGG 179365 0.0 82.18543 3 CAGTGGT 181355 0.0 80.70968 4 AGCAGTG 185055 0.0 79.67065 2 CAACGCA 170100 0.0 44.289814 12-13 ATCAACG 171055 0.0 44.12828 10-11 ACGCAGA 171945 0.0 43.83752 14-15 AGAGTAC 170915 0.0 43.456356 18-19 GAGTACT 165885 0.0 43.246708 20-21 TCAACGC 169775 0.0 43.163273 12-13 AACGCAG 171265 0.0 42.83947 14-15 CGCAGAG 173270 0.0 42.306877 16-17 AGTACTT 174095 0.0 42.303402 20-21 TATCAAC 174555 0.0 41.794792 10-11 GTACTTT 174480 0.0 41.043034 22-23 >>END_MODULE