FastQCFastQC Report
Thu 26 May 2016
SRR1660732_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660732_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22899761
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT12648105.523245417277499No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT351210.15336841288430914No Hit
TAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT282310.12328076262455316No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA278330.12154275321912748No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1653100.084.4015961
GGTATCA1714550.084.104458
AGTGGTA1705250.084.016835
TGGTATC1733600.083.178667
GCAGTGG1726600.082.95923
GTGGTAT1727400.082.928626
GTATCAA1739800.082.781139
AGCAGTG1770400.080.6157152
CAGTGGT1802700.079.36624
CAACGCA1692700.043.9772112-13
ATCAACG1702050.043.80737710-11
ACGCAGA1713250.043.55122814-15
AGAGTAC1723350.043.14543518-19
GAGTACT1666600.042.85827320-21
TCAACGC1688150.042.6137412-13
AACGCAG1705600.042.2742214-15
AGTACTT1751350.042.15526620-21
CGCAGAG1725150.041.78594616-17
TATCAAC1740900.041.34953310-11
GTACTTT1753150.040.75626422-23
Produced by FastQC (version 0.11.5)