FastQCFastQC Report
Thu 26 May 2016
SRR1660731_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660731_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26917922
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT12320484.577054647829057No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT386270.14349918987059995No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1594050.083.940378
AGTGGTA1609250.083.350845
TGGTATC1618650.082.4167567
GTATCAA1630400.082.004239
GTGGTAT1639850.081.761966
AAGCAGT1667150.080.783791
GCAGTGG1697750.079.3891753
CAGTGGT1723100.077.7300344
AGCAGTG1757100.076.796492
CAACGCA1562900.044.4276212-13
ATCAACG1575800.044.14000710-11
ACGCAGA1592950.043.6411514-15
TCAACGC1562700.042.92923412-13
AGAGTAC1599450.042.8300418-19
AACGCAG1575900.042.6708614-15
GAGTACT1553650.042.3661720-21
CGCAGAG1600800.041.95065316-17
AGTACTT1641950.041.48200620-21
TATCAAC1621350.041.22580310-11
GTACTTT1657950.039.7155622-23
Produced by FastQC (version 0.11.5)