##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660731_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 26917922 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.877096827905216 34.0 31.0 34.0 30.0 34.0 2 31.999645217784643 34.0 31.0 34.0 30.0 34.0 3 32.05922292218545 34.0 31.0 34.0 30.0 34.0 4 35.46637812532483 37.0 35.0 37.0 33.0 37.0 5 35.438280265467746 37.0 35.0 37.0 33.0 37.0 6 35.50485178610741 37.0 35.0 37.0 33.0 37.0 7 35.47021553149608 37.0 35.0 37.0 33.0 37.0 8 35.47129663277871 37.0 35.0 37.0 33.0 37.0 9 37.15174377130597 39.0 37.0 39.0 34.0 39.0 10-11 37.105748653257855 39.0 37.0 39.0 33.0 39.0 12-13 37.11208107000236 39.0 37.0 39.0 33.0 39.0 14-15 38.48369248190852 40.0 38.0 41.0 34.0 41.0 16-17 38.46374508403731 40.0 38.0 41.0 34.0 41.0 18-19 38.43268783897955 40.0 38.0 41.0 33.5 41.0 20-21 38.32234520183245 40.0 38.0 41.0 33.5 41.0 22-23 38.16289439058483 40.0 38.0 41.0 33.0 41.0 24-25 38.137960445089334 40.0 38.0 41.0 33.0 41.0 26-27 38.10594686692383 40.0 38.0 41.0 33.0 41.0 28-29 38.015218002340596 40.0 38.0 41.0 33.0 41.0 30-31 37.92102735493475 40.0 38.0 41.0 32.5 41.0 32-33 37.79345227688824 40.0 38.0 41.0 32.0 41.0 34-35 37.636069363006555 40.0 37.5 41.0 31.5 41.0 36-37 37.451614095620016 40.0 37.0 41.0 31.0 41.0 38-39 37.072410641504945 40.0 36.0 41.0 31.0 41.0 40-41 37.011093464049715 40.0 35.5 41.0 30.5 41.0 42-43 37.02499041345019 40.0 35.5 41.0 30.5 41.0 44-45 37.027388146826496 40.0 35.0 41.0 31.0 41.0 46-47 37.123394796225355 40.0 36.0 41.0 31.0 41.0 48-49 37.021002884249384 40.0 35.0 41.0 30.5 41.0 50-51 35.658431490365416 38.5 34.0 40.0 28.5 40.5 52-53 35.83505166186305 38.5 35.0 39.5 28.5 40.5 54-55 36.29452854867475 39.5 35.0 41.0 28.5 41.0 56-57 35.989261856840216 39.5 35.0 41.0 26.5 41.0 58-59 35.81188910124638 39.0 35.0 41.0 26.0 41.0 60-61 35.58659032075359 39.0 35.0 41.0 26.0 41.0 62-63 35.35579568512013 39.0 35.0 41.0 26.0 41.0 64-65 35.10073736375341 38.0 35.0 40.5 25.5 41.0 66-67 34.82880080787811 37.0 34.0 40.0 25.0 41.0 68-69 34.54185967624098 37.0 34.0 40.0 25.0 41.0 70-71 34.231793245407275 36.0 34.0 39.0 25.0 41.0 72-73 33.88716199935493 36.0 34.0 39.0 24.0 41.0 74-75 33.51036697780758 35.0 33.0 38.5 24.0 40.5 76-77 33.118509166495095 35.0 33.0 37.0 23.0 39.5 78-79 32.75101328401204 35.0 33.0 37.0 22.5 39.0 80-81 32.42868539406571 35.0 33.0 36.5 21.5 39.0 82-83 32.11251882667615 35.0 32.5 36.0 20.0 38.0 84-85 31.823908825502947 35.0 32.0 36.0 20.0 37.0 86-87 31.60259722128625 35.0 32.0 35.0 20.0 37.0 88-89 31.391280928000313 35.0 32.0 35.0 19.5 36.0 90-91 31.214547337643673 35.0 32.0 35.0 19.0 36.0 92-93 31.057424362103433 35.0 31.5 35.0 18.5 36.0 94-95 30.87388863077915 35.0 31.0 35.0 18.0 35.5 96-97 30.735586220214174 35.0 31.0 35.0 17.5 35.0 98-99 30.628032635654417 35.0 31.0 35.0 17.0 35.0 100 30.543766714235964 34.0 31.0 35.0 17.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5548302622728869 1101 2 0.5890292413901932 1101 3 0.5718131017898784 1101 4 0.4951017901982411 1101 5 0.5197521296007181 1101 6 0.4936075037637977 1101 7 0.5032750705513038 1101 8 0.5116833682191526 1101 9 0.5920528997555863 1101 10-11 0.6063291829454585 1101 12-13 0.6114115336782291 1101 14-15 0.7451321981586929 1101 16-17 0.6994574975390364 1101 18-19 0.7204757032432738 1101 20-21 0.7529052428742915 1101 22-23 0.8074551475175866 1101 24-25 0.799304302479122 1101 26-27 0.7662549379326506 1101 28-29 0.7477831347105237 1101 30-31 0.7232491351731127 1101 32-33 0.7074876329093271 1101 34-35 0.7178139872771183 1101 36-37 0.698820520072978 1101 38-39 0.712599069034475 1101 40-41 0.7003041911509129 1101 42-43 0.7095343174687727 1101 44-45 0.7722572466922415 1101 46-47 0.803818425508041 1101 48-49 0.8225341630650362 1101 50-51 0.7485426143951344 1101 52-53 0.7664053161967956 1101 54-55 0.8246345435066402 1101 56-57 0.8553160689156982 1101 58-59 0.7741601274914203 1101 60-61 0.751549153820747 1101 62-63 0.7197326119315974 1101 64-65 0.6855131526679727 1101 66-67 0.7082599038248532 1101 68-69 0.6843472665502404 1101 70-71 0.6386392299964498 1101 72-73 0.6093151268427022 1101 74-75 0.5784757020905005 1101 76-77 0.5992844371175181 1101 78-79 0.6002179305403885 1101 80-81 0.570555601899386 1101 82-83 0.5799987756238565 1101 84-85 0.5565453028636931 1101 86-87 0.47581635003659883 1101 88-89 0.4755063958879724 1101 90-91 0.4334798422074151 1101 92-93 0.4596647920962589 1101 94-95 0.5032779597719674 1101 96-97 0.4570699244085077 1101 98-99 0.4145966820402087 1101 100 0.403941774166789 1102 1 0.4386807191825568 1102 2 0.47021180792967954 1102 3 0.47962683822301955 1102 4 0.4212215494421869 1102 5 0.39626293269373747 1102 6 0.3652218138128447 1102 7 0.39616758948729824 1102 8 0.43600951741912297 1102 9 0.5060255664399236 1102 10-11 0.4944992517685307 1102 12-13 0.5087918798342415 1102 14-15 0.5702505284632693 1102 16-17 0.5670304720625055 1102 18-19 0.5337502361389213 1102 20-21 0.54996634844494 1102 22-23 0.594901497621251 1102 24-25 0.5683497771310826 1102 26-27 0.5546086403752142 1102 28-29 0.5558467903305271 1102 30-31 0.4958668411067819 1102 32-33 0.4819789981904421 1102 34-35 0.5085692713193879 1102 36-37 0.523257306283547 1102 38-39 0.529466351916291 1102 40-41 0.5142790785799036 1102 42-43 0.496305937015876 1102 44-45 0.5341245620310957 1102 46-47 0.5720293540804491 1102 48-49 0.5828143390436793 1102 50-51 0.5082880399417888 1102 52-53 0.5453716186174091 1102 54-55 0.5757815922858569 1102 56-57 0.5878561132124744 1102 58-59 0.492174232197236 1102 60-61 0.4891465414050771 1102 62-63 0.49459403136422253 1102 64-65 0.4634343775350658 1102 66-67 0.4223774216297329 1102 68-69 0.43235764284541034 1102 70-71 0.4133202127057345 1102 72-73 0.3773500113735011 1102 74-75 0.4088420034275799 1102 76-77 0.4021738553082912 1102 78-79 0.3650526000054839 1102 80-81 0.311602894340659 1102 82-83 0.299501770041779 1102 84-85 0.33631069754151 1102 86-87 0.2918807580741678 1102 88-89 0.24759030801813253 1102 90-91 0.28174718062249227 1102 92-93 0.2847407086183189 1102 94-95 0.27835810547038875 1102 96-97 0.2349686099375461 1102 98-99 0.16866201869069997 1102 100 0.15656657476954194 1103 1 0.378693460548881 1103 2 0.4018948210211164 1103 3 0.42706480632347876 1103 4 0.36438335827292434 1103 5 0.35734410067347966 1103 6 0.3613150701510648 1103 7 0.3523188239626691 1103 8 0.38637280593441403 1103 9 0.44262758744410036 1103 10-11 0.42253755909125346 1103 12-13 0.46162194404002577 1103 14-15 0.5095420548483887 1103 16-17 0.5205191193464387 1103 18-19 0.5084412110506165 1103 20-21 0.5359614688450733 1103 22-23 0.5326110431332793 1103 24-25 0.5885617510595935 1103 26-27 0.5357343623887445 1103 28-29 0.5253694620620664 1103 30-31 0.501735805195274 1103 32-33 0.46919440804931867 1103 34-35 0.4855504529907577 1103 36-37 0.47923370250799735 1103 38-39 0.4902106939624815 1103 40-41 0.4570483891740835 1103 42-43 0.45963715980123254 1103 44-45 0.48059951289944536 1103 46-47 0.5122789161460162 1103 48-49 0.5454321151180039 1103 50-51 0.46256864959556054 1103 52-53 0.46058343967219173 1103 54-55 0.4876289949630248 1103 56-57 0.5303643969760827 1103 58-59 0.47777176396702004 1103 60-61 0.4645155236683962 1103 62-63 0.41944737400653054 1103 64-65 0.4194321427991099 1103 66-67 0.3726907282168668 1103 68-69 0.35213859471775066 1103 70-71 0.33879386917121934 1103 72-73 0.32411775298579926 1103 74-75 0.3309667150892466 1103 76-77 0.3494380537897115 1103 78-79 0.32443569213160117 1103 80-81 0.25618407839036195 1103 82-83 0.2383456215238624 1103 84-85 0.2227425357697861 1103 86-87 0.19434342924675363 1103 88-89 0.17493173332353962 1103 90-91 0.17069585562734702 1103 92-93 0.15459712901939682 1103 94-95 0.23230403095196905 1103 96-97 0.21757833601148135 1103 98-99 0.16979263482237528 1103 100 0.1751347697785981 1104 1 0.37771270690478786 1104 2 0.4078414165187212 1104 3 0.3912177475855714 1104 4 0.35130029870809665 1104 5 0.3370181033311752 1104 6 0.30990158088101794 1104 7 0.328360516380485 1104 8 0.3416537786441225 1104 9 0.38591948444026514 1104 10-11 0.35737337885750975 1104 12-13 0.37506488851412456 1104 14-15 0.48127723671755973 1104 16-17 0.43218611877394153 1104 18-19 0.41879044794955433 1104 20-21 0.3629907180062304 1104 22-23 0.4446116605788504 1104 24-25 0.4535835453550945 1104 26-27 0.3944941206015855 1104 28-29 0.3811415187043039 1104 30-31 0.3774513903094743 1104 32-33 0.3652633142773425 1104 34-35 0.35034851655720445 1104 36-37 0.34627291847790076 1104 38-39 0.3386138275449966 1104 40-41 0.34443905122898144 1104 42-43 0.37057094724519146 1104 44-45 0.4088780506221781 1104 46-47 0.39246954064583406 1104 48-49 0.4161253164592651 1104 50-51 0.39424046881455155 1104 52-53 0.38100596154752253 1104 54-55 0.49965685239686053 1104 56-57 0.5129534604505466 1104 58-59 0.46334703756922835 1104 60-61 0.48739266977013074 1104 62-63 0.48312623025166346 1104 64-65 0.4908444995483734 1104 66-67 0.5012710032519863 1104 68-69 0.46510940272435874 1104 70-71 0.4678248989519673 1104 72-73 0.4691236078705998 1104 74-75 0.39618072785254554 1104 76-77 0.3596439459536924 1104 78-79 0.3760049080565224 1104 80-81 0.377259925253405 1104 82-83 0.3615208664230707 1104 84-85 0.3608363033559563 1104 86-87 0.3326732275492681 1104 88-89 0.29817776666007134 1104 90-91 0.2817241699289106 1104 92-93 0.3240998504088317 1104 94-95 0.33749243881270985 1104 96-97 0.33054896400185996 1104 98-99 0.336372785185322 1104 100 0.3263457495410087 1105 1 0.24748039586880566 1105 2 0.32251696992771883 1105 3 0.29647657395601357 1105 4 0.2294337608348016 1105 5 0.250692996674168 1105 6 0.2363443402789045 1105 7 0.25242761772476996 1105 8 0.26271007744473707 1105 9 0.25389391895689073 1105 10-11 0.29007227606430774 1105 12-13 0.27751225227488163 1105 14-15 0.3156371497820345 1105 16-17 0.30596089137316085 1105 18-19 0.3060903916024529 1105 20-21 0.3156559357475146 1105 22-23 0.36637545354350465 1105 24-25 0.3780382181394444 1105 26-27 0.3689504355013682 1105 28-29 0.3625550259086836 1105 30-31 0.3308382372868479 1105 32-33 0.3410887348681797 1105 34-35 0.3460918313764978 1105 36-37 0.32554906755623136 1105 38-39 0.36424580049838795 1105 40-41 0.36446403714220565 1105 42-43 0.3945578425200793 1105 44-45 0.41300030772593743 1105 46-47 0.4274624087131258 1105 48-49 0.41427668086399194 1105 50-51 0.3269425214588395 1105 52-53 0.39006802385131323 1105 54-55 0.5028808401614882 1105 56-57 0.5928719020508026 1105 58-59 0.49971722190473855 1105 60-61 0.4953902548945024 1105 62-63 0.46915897596525014 1105 64-65 0.4536985605642343 1105 66-67 0.40121021278270064 1105 68-69 0.4232129525915411 1105 70-71 0.44913978263545573 1105 72-73 0.41327922245644544 1105 74-75 0.4267673494415334 1105 76-77 0.438363611090935 1105 78-79 0.4485318339198443 1105 80-81 0.39671857453296866 1105 82-83 0.3762043728224924 1105 84-85 0.3772859210006523 1105 86-87 0.31771635722808966 1105 88-89 0.3488200591820103 1105 90-91 0.34047274917708137 1105 92-93 0.30116632388544673 1105 94-95 0.3902811693930879 1105 96-97 0.3455867165576052 1105 98-99 0.3308083643529933 1105 100 0.33240676539869085 1106 1 0.2089102833565306 1106 2 0.265618200766653 1106 3 0.22972686467564074 1106 4 0.22371511702297653 1106 5 0.1932912599075749 1106 6 0.18252312152182526 1106 7 0.18736006505543656 1106 8 0.21812815491963278 1106 9 0.2006036977079262 1106 10-11 0.186864966542295 1106 12-13 0.21404645147446644 1106 14-15 0.27575636748195365 1106 16-17 0.2453240266144121 1106 18-19 0.20985274152316435 1106 20-21 0.2624308356946585 1106 22-23 0.2518571721873215 1106 24-25 0.259127367123817 1106 26-27 0.2687697513663565 1106 28-29 0.25917210663057233 1106 30-31 0.26802718238992895 1106 32-33 0.26424382057383866 1106 34-35 0.2776634390182693 1106 36-37 0.20781655981628688 1106 38-39 0.23849891535152068 1106 40-41 0.2619905492618173 1106 42-43 0.23083986321025662 1106 44-45 0.28052784680318155 1106 46-47 0.2774856360785236 1106 48-49 0.2780854553108725 1106 50-51 0.1678628869349481 1106 52-53 0.22121670617404732 1106 54-55 0.3444956102286909 1106 56-57 0.3956440231713927 1106 58-59 0.3208386596454247 1106 60-61 0.314807003365388 1106 62-63 0.2624258490302651 1106 64-65 0.23828090595425522 1106 66-67 0.2267688626354456 1106 68-69 0.20188422043116105 1106 70-71 0.2225101065801809 1106 72-73 0.22745175133962903 1106 74-75 0.23132359802569624 1106 76-77 0.22404431787942514 1106 78-79 0.23563203780113895 1106 80-81 0.16425487295477836 1106 82-83 0.2206353023701766 1106 84-85 0.21331419254032724 1106 86-87 0.18330475864771145 1106 88-89 0.17685319366815477 1106 90-91 0.16378260343971007 1106 92-93 0.14914572432341444 1106 94-95 0.20072892292325406 1106 96-97 0.21626752906232483 1106 98-99 0.20743668119962777 1106 100 0.1709300607245261 1107 1 0.18628186015397574 1107 2 0.20611639537078474 1107 3 0.2042675254837647 1107 4 0.20507435184318012 1107 5 0.20272081418394094 1107 6 0.16108472848681288 1107 7 0.1527956731409077 1107 8 0.18843942133234037 1107 9 0.1981206418948389 1107 10-11 0.195230665990195 1107 12-13 0.2003585668226009 1107 14-15 0.20599871664889946 1107 16-17 0.20441872299812758 1107 18-19 0.1772346315092861 1107 20-21 0.19246042915939654 1107 22-23 0.1926489522381445 1107 24-25 0.2424098985585843 1107 26-27 0.19387969055827625 1107 28-29 0.23970670520855464 1107 30-31 0.1994191836357757 1107 32-33 0.1665379429869489 1107 34-35 0.26297459813284974 1107 36-37 0.24362514180803174 1107 38-39 0.24315113629173624 1107 40-41 0.217632941069148 1107 42-43 0.2680003741238224 1107 44-45 0.27589734744302774 1107 46-47 0.2700674562508709 1107 48-49 0.3248416586518772 1107 50-51 0.25697783663459006 1107 52-53 0.23754311616860946 1107 54-55 0.3269814334616967 1107 56-57 0.3480207696815185 1107 58-59 0.2787584373929022 1107 60-61 0.28819525641794996 1107 62-63 0.25688832020818353 1107 64-65 0.2595283556229404 1107 66-67 0.3170771770914058 1107 68-69 0.29139113488456303 1107 70-71 0.29521342932202543 1107 72-73 0.28794516236958856 1107 74-75 0.2824266069768271 1107 76-77 0.24224772114506266 1107 78-79 0.2640663788446318 1107 80-81 0.19835480821810592 1107 82-83 0.22183742850068455 1107 84-85 0.21425116385932697 1107 86-87 0.20643104351805164 1107 88-89 0.2375950707522101 1107 90-91 0.262860590742207 1107 92-93 0.21722632795389174 1107 94-95 0.24597992176597572 1107 96-97 0.2738538730959412 1107 98-99 0.24280795146792045 1107 100 0.2616548721945051 1108 1 0.1400196117650907 1108 2 0.17331239948819643 1108 3 0.1886223310475188 1108 4 0.1700595528858031 1108 5 0.1615869948662123 1108 6 0.11072755629515285 1108 7 0.13745581409772 1108 8 0.164278994343114 1108 9 0.16355157127274111 1108 10-11 0.11568091654277168 1108 12-13 0.1528854251487175 1108 14-15 0.1834417742031249 1108 16-17 0.15282578750147735 1108 18-19 0.14604033783509607 1108 20-21 0.15204996692830264 1108 22-23 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2316 94-95 -1.268085144266415 2316 96-97 -1.4502702395648726 2316 98-99 -1.2620598267786356 2316 100 -1.0373245068333503 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 13958.0 3 3363.0 4 2471.0 5 2022.0 6 3296.0 7 15121.0 8 29878.0 9 36246.0 10 37722.0 11 36132.0 12 34883.0 13 36938.0 14 42676.0 15 51989.0 16 65330.0 17 80703.0 18 89461.0 19 100053.0 20 115146.0 21 140801.0 22 181861.0 23 261461.0 24 374312.0 25 448369.0 26 439502.0 27 387902.0 28 364382.0 29 367256.0 30 414819.0 31 501523.0 32 627642.0 33 812343.0 34 1120502.0 35 1697929.0 36 2950432.0 37 5585644.0 38 7192849.0 39 2246199.0 40 4806.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 14.74155030575327 32.393665243438804 27.509371639131164 25.355412811676754 2 20.44058566938599 32.27179973950956 29.282868867633354 18.004745723471096 3 23.92763705712888 26.036597292245144 27.571323651339867 22.4644419992861 4 18.370261452685284 28.629592848201806 25.684382971699947 27.315762727412963 5 21.741360908084168 32.00517769772308 26.890325515688268 19.36313587850448 6 26.961569074525283 27.86560148443136 26.47909020863548 18.693739232407882 7 20.848869816740642 26.704020816423206 32.02599389317759 20.421115473658556 8 25.442964133620094 26.832012065705115 27.015062413758596 20.709961386916195 9 24.86350157221041 25.187946905171344 28.95785919574758 20.990692326870665 10-11 20.512258709046808 29.534149191831823 29.372284177128456 20.581307921992913 12-13 20.485873573837058 27.057290103105654 29.116817784082922 23.340018538974366 14-15 20.465497092166142 32.32390418168888 26.549045527631904 20.661553198513076 16-17 23.22985378944381 26.80603875784962 26.630819136198554 23.333288316508018 18-19 20.49926333402255 29.5018603527476 26.67365795800738 23.325218355222464 20-21 23.180007336047524 29.66580434627335 26.599373683082746 20.554814634596383 22-23 23.20029682666561 26.976077414417365 29.22107171829803 20.60255404061899 24-25 20.601495240367605 29.459803527497318 26.460394641019434 23.47830659111564 26-27 20.64681562160862 26.84740860480992 31.885692864061415 20.62008290952004 28-29 20.606284480837097 26.967397664069825 31.875205316676503 20.55111253841658 30-31 20.558497955166715 26.856632440736206 31.87889352320676 20.705976080890323 32-33 20.697967156070586 27.02511049018811 31.637537069001613 20.639385284739692 34-35 20.71712695509846 27.157664257817476 31.491015584520436 20.634193202563623 36-37 20.63543550445004 27.091396044070137 31.597068436493263 20.676100014986552 38-39 20.70085085848467 27.064566955077122 31.62396445682302 20.61061772961518 40-41 20.711110534290757 27.09986056896816 31.597415003558478 20.591613893182608 42-43 20.70459219876084 27.12772304655275 31.55396733890562 20.613717415780794 44-45 20.7388097752877 27.20400032773239 31.526740747470743 20.530449149509167 46-47 20.712678870358907 27.308370765163524 31.457414811543522 20.521535552934044 48-49 20.801296504470677 27.307387441051816 31.328607532148844 20.562708522328663 50-51 20.828869924466602 27.372069124759584 31.27969205411919 20.519368896654626 52-53 20.943388406263253 27.486321578227386 31.03394307365971 20.53634694184965 54-55 21.247676897472655 27.68306278491744 30.38044268340363 20.688817634206274 56-57 21.682654081651076 28.15473583087249 29.412755160662357 20.74985492681407 58-59 21.831922322809355 28.58859346791919 28.820863469602582 20.758620739668878 60-61 22.00780441911998 28.77935845145247 28.49791122202237 20.71492590740518 62-63 22.07092248274434 28.91449816144913 28.349683776391515 20.664895579415013 64-65 22.184799060009215 28.97278022381854 28.256809565298518 20.585611150873724 66-67 22.28149083129015 28.80817809970392 28.406112424879222 20.504218644126713 68-69 22.39066178249682 28.67896691293037 28.529444373447237 20.400926931125575 70-71 22.535528862953633 28.574881318752116 28.585106977351117 20.304482840943137 72-73 22.69332564582285 28.513899761076967 28.543810236813833 20.248964356286354 74-75 22.919499290639923 28.59927623179952 28.389465870790914 20.091758606769645 76-77 23.220467949868652 28.685622517825887 28.078373823243307 20.015535709062153 78-79 23.503635922842932 28.812818002500084 27.746970051900206 19.936576022756775 80-81 23.805452754388632 28.954871386888982 27.424700108029242 19.814975750693144 82-83 24.003745659478966 29.084807459513172 27.12842244600408 19.783024435003785 84-85 24.172424427804355 29.11152645212814 26.984538176031727 19.731510944035783 86-87 24.37739480882392 29.067365420983243 26.90017444926953 19.655065320923306 88-89 24.617410285440656 29.06841824451799 26.74347229992135 19.570699170120005 90-91 24.822661169282124 29.038560719272045 26.629379594750034 19.5093985166958 92-93 25.01082160690306 29.04355968830052 26.534602448482737 19.41101625631369 94-95 25.213975277704442 28.99826153282488 26.446952236364872 19.3408109531058 96-97 25.383745122959006 28.93913132560863 26.356833057756674 19.32029049367569 98-99 25.56753950813258 28.92130087451284 26.27991534089158 19.231244276463 100 25.643963156217847 28.898561469026085 26.194584599902505 19.262890774853563 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 27599.0 1 20669.0 2 11708.0 3 9108.5 4 8490.5 5 8526.0 6 8716.0 7 8875.5 8 8916.0 9 9032.0 10 9707.0 11 12162.0 12 91192.0 13 112982.0 14 56722.0 15 59385.0 16 66762.5 17 74601.5 18 82294.5 19 89777.5 20 99773.5 21 115449.0 22 137655.0 23 164665.5 24 192808.0 25 223944.5 26 260804.0 27 304813.0 28 358594.0 29 412851.0 30 459799.5 31 505071.0 32 552092.5 33 592277.0 34 625286.0 35 664059.5 36 709267.0 37 752060.0 38 796702.0 39 826979.0 40 835403.5 41 855369.5 42 887661.5 43 894773.0 44 890700.5 45 899742.5 46 907813.5 47 909330.5 48 900657.5 49 886828.0 50 874270.0 51 859320.0 52 835891.5 53 796378.5 54 744665.5 55 691896.0 56 636999.0 57 576288.5 58 511066.0 59 438872.0 60 360912.0 61 291933.0 62 230010.5 63 172887.0 64 128307.0 65 93311.5 66 67009.0 67 49541.0 68 37093.5 69 27705.0 70 21009.0 71 16482.5 72 13447.0 73 10987.5 74 9005.0 75 7606.5 76 6194.5 77 4412.0 78 3101.5 79 2409.0 80 1924.5 81 1606.0 82 1356.0 83 1132.0 84 932.0 85 802.5 86 717.5 87 617.5 88 523.0 89 450.5 90 394.5 91 344.0 92 299.5 93 248.5 94 217.5 95 199.0 96 149.0 97 113.0 98 99.0 99 71.5 100 41.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.40633894399426523 2 0.3859361803634025 3 0.17003541358058769 4 0.1763769134928023 5 0.27613944345332453 6 0.10262679266252424 7 0.13685677519980924 8 0.14277476545180567 9 0.1361917907333263 10-11 0.132424783755596 12-13 0.13439373217590866 14-15 0.14065535965220494 16-17 0.14519322851147276 18-19 0.1564199495042745 20-21 0.13731371983320256 22-23 0.09958792510060768 24-25 0.09457082162582982 26-27 0.08880886124865062 28-29 0.09858116090833462 30-31 0.10339765454406176 32-33 0.10477777593679036 34-35 0.10545947788986089 36-37 0.10086959907232065 38-39 0.10038850695830087 40-41 0.10107763890541031 42-43 0.06378649882409199 44-45 0.06659503657080217 46-47 0.08514587418746514 48-49 0.08941812075984172 50-51 0.09872047329656428 52-53 0.10414065394795334 54-55 0.1068916092408619 56-57 0.107391276339979 58-59 0.10677458683474898 60-61 0.10460131357836612 62-63 0.10905002250916694 64-65 0.11618838928205528 66-67 0.12494463725691754 68-69 0.13009362312588615 70-71 0.12842001696862038 72-73 0.11524292254060325 74-75 0.11259412966572978 76-77 0.11454264560243543 78-79 0.11314580672311927 80-81 0.10738570384444981 82-83 0.11045800637954149 84-85 0.1163314166673044 86-87 0.13015492057670722 88-89 0.1380790092192109 90-91 0.14518022602190467 92-93 0.14424218927449156 94-95 0.13446431711927836 96-97 0.13077718257746643 98-99 0.12582137655350958 100 0.11999440372848988 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2.6917922E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.66956884125334 #Duplication Level Percentage of deduplicated Percentage of total 1 69.20216833747807 28.14422349165066 2 15.849638595146484 12.891959359085913 3 5.683487075646794 6.934349066441727 4 2.6705293471946923 4.344371085132874 5 1.4902195707269643 3.030329371013163 6 0.9914193016712675 2.419235732392015 7 0.6682163626264507 1.902324995248184 8 0.5004658221332379 1.6282983364753731 9 0.39696475910798196 1.4529947038284257 >10 2.202308485860443 16.443487252980717 >50 0.20154892949011388 5.746284015222083 >100 0.14153610522366608 9.605156631719641 >500 9.482209415516468E-4 0.23632282620342734 >1k 5.1258764134651E-4 0.40303284194554745 >5k 9.12477776023244E-6 0.027146021858098612 >10k+ 2.7374333280695203E-5 4.790484268802312 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 1232048 4.577054647829057 No Hit AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 38627 0.14349918987059995 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 5.312445737824785E-4 1.4859988077831566E-5 0.0 0.0 1.4859988077831566E-5 2 5.461045618603101E-4 1.8574985097289455E-5 0.0 0.0 1.4859988077831566E-5 3 5.461045618603101E-4 1.8574985097289455E-5 0.0 0.0 1.4859988077831566E-5 4 5.758245380159732E-4 4.457996423349469E-5 0.0 0.0 2.9719976155663132E-5 5 5.758245380159732E-4 5.9439952311326264E-5 0.0 0.0 2.9719976155663132E-5 6 5.906845260938048E-4 7.429994038915782E-5 0.0 0.0 3.343497317512102E-5 7 5.906845260938048E-4 8.17299344280736E-5 0.0 0.0 3.343497317512102E-5 8 5.981145201327205E-4 9.287492548644728E-5 0.0 0.0 3.343497317512102E-5 9 6.204045022494679E-4 1.0030491952536306E-4 0.0 0.0 3.343497317512102E-5 10-11 6.445519828759442E-4 1.1330740909346568E-4 0.0 0.0 4.457996423349469E-5 12-13 6.928469441288967E-4 1.3559739121021304E-4 0.0 0.0 5.015245976268153E-5 14-15 7.244244187942888E-4 1.4488488375885775E-4 0.0 0.0 5.2009958272410475E-5 16-17 7.745768785569704E-4 1.6903236438533403E-4 0.0 0.0 5.5724955291868366E-5 18-19 8.395893263974834E-4 1.8946484799235244E-4 0.0 0.0 6.12974508210552E-5 20-21 9.120317682769124E-4 2.080398330896419E-4 0.0 0.0 6.686994635024204E-5 22-23 0.0010141941863120044 2.359023107355761E-4 0.0 1.8574985097289457E-6 7.058494336969993E-5 24-25 0.0013113939478686357 2.693372839106971E-4 0.0 3.7149970194578915E-6 7.058494336969993E-5 26-27 0.002362738104375219 2.8791226900798654E-4 0.0 7.429994038915783E-6 7.244244187942888E-5 28-29 0.00546290311711283 3.1205974963446285E-4 0.0 1.1144991058373673E-5 7.615743889888677E-5 30-31 0.013405566744713802 3.41779725790126E-4 1.8574985097289457E-6 1.1144991058373673E-5 7.801493740861571E-5 32-33 0.02998188344553491 3.6035471088741546E-4 3.7149970194578915E-6 1.3002489568102619E-5 8.17299344280736E-5 34-35 0.060407709034895035 3.826446930041628E-4 3.7149970194578915E-6 1.4859988077831566E-5 8.915992846698939E-5 36-37 0.1095218271306381 3.900746870430786E-4 3.7149970194578915E-6 1.8574985097289455E-5 9.658992250590517E-5 38-39 0.1811321096777084 4.030771766111812E-4 3.7149970194578915E-6 1.8574985097289455E-5 9.844742101563411E-5 40-41 0.2778297670971779 4.0679217363063906E-4 3.7149970194578915E-6 3.1577474665392074E-5 1.0959241207400779E-4 42-43 0.40332608141148485 4.216521617084706E-4 3.7149970194578915E-6 3.343497317512102E-5 1.1516490760319462E-4 44-45 0.5638771076013966 4.365121497863022E-4 3.7149970194578915E-6 3.343497317512102E-5 1.1887990462265251E-4 46-47 0.7640838694755114 4.4579964233494693E-4 3.7149970194578915E-6 3.714997019457891E-5 1.225949016421104E-4 48-49 1.0109194164393522 4.718046214711522E-4 3.7149970194578915E-6 4.272246572376575E-5 1.225949016421104E-4 50-51 1.2969333219703958 4.940946035878995E-4 3.7149970194578915E-6 4.643746274322364E-5 1.3559739121021304E-4 52-53 1.6162094533151556 5.070970931560022E-4 3.7149970194578915E-6 4.8294961252952584E-5 1.4116988673939986E-4 54-55 1.9797832091199314 5.293870752727495E-4 3.7149970194578915E-6 5.015245976268153E-5 1.4488488375885775E-4 56-57 2.394480896407977 5.516770573894969E-4 3.7149970194578915E-6 5.2009958272410475E-5 1.4674238226858668E-4 58-59 2.8653530536272447 5.906845260938048E-4 3.7149970194578915E-6 5.2009958272410475E-5 1.504573792880446E-4 60-61 3.3833499480383367 6.352644903272995E-4 3.7149970194578915E-6 5.2009958272410475E-5 1.5974487183668932E-4 62-63 3.9374398959919716 6.48266979895402E-4 3.7149970194578915E-6 5.2009958272410475E-5 1.6531736736587617E-4 64-65 4.528354751900983 6.705569620121494E-4 3.7149970194578915E-6 5.2009958272410475E-5 1.70889862895063E-4 66-67 5.163457639857935 6.85416950089981E-4 3.7149970194578915E-6 5.386745678213942E-5 1.8574985097289455E-4 68-69 5.84548651266617 6.984194396580835E-4 3.7149970194578915E-6 5.5724955291868366E-5 1.9317984501181034E-4 70-71 6.571621687587919 7.058494336969994E-4 3.7149970194578915E-6 5.5724955291868366E-5 1.9689484203126823E-4 72-73 7.3259091099231215 7.318544128332046E-4 3.7149970194578915E-6 5.5724955291868366E-5 2.0060983905072612E-4 74-75 8.107943844996653 7.615743889888677E-4 3.7149970194578915E-6 5.5724955291868366E-5 2.0618233457991294E-4 76-77 8.922209894211 8.098693502418203E-4 3.7149970194578915E-6 5.5724955291868366E-5 2.080398330896419E-4 78-79 9.767093091361213 8.340168308682966E-4 3.7149970194578915E-6 5.5724955291868366E-5 2.117548301090998E-4 80-81 10.63823945994048 8.488768189461282E-4 3.7149970194578915E-6 5.5724955291868366E-5 2.1546982712855768E-4 82-83 11.532169905240085 8.89741786160165E-4 3.7149970194578915E-6 5.5724955291868366E-5 2.3032981520638925E-4 84-85 12.448213870298012 9.083167712574544E-4 3.7149970194578915E-6 5.5724955291868366E-5 2.3032981520638925E-4 86-87 13.378881921123035 9.250342578450149E-4 3.7149970194578915E-6 5.5724955291868366E-5 2.3404481222584714E-4 88 14.086930633055553 9.324642518839307E-4 3.7149970194578915E-6 5.5724955291868366E-5 2.3404481222584714E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 159405 0.0 83.94037 8 AGTGGTA 160925 0.0 83.35084 5 TGGTATC 161865 0.0 82.416756 7 GTATCAA 163040 0.0 82.00423 9 GTGGTAT 163985 0.0 81.76196 6 AAGCAGT 166715 0.0 80.78379 1 GCAGTGG 169775 0.0 79.389175 3 CAGTGGT 172310 0.0 77.730034 4 AGCAGTG 175710 0.0 76.79649 2 CAACGCA 156290 0.0 44.42762 12-13 ATCAACG 157580 0.0 44.140007 10-11 ACGCAGA 159295 0.0 43.64115 14-15 TCAACGC 156270 0.0 42.929234 12-13 AGAGTAC 159945 0.0 42.83004 18-19 AACGCAG 157590 0.0 42.67086 14-15 GAGTACT 155365 0.0 42.36617 20-21 CGCAGAG 160080 0.0 41.950653 16-17 AGTACTT 164195 0.0 41.482006 20-21 TATCAAC 162135 0.0 41.225803 10-11 GTACTTT 165795 0.0 39.71556 22-23 >>END_MODULE