FastQCFastQC Report
Thu 26 May 2016
SRR1660731_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660731_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26917922
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT12261334.555080440458963No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT413380.15357054679035032No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1668400.083.1845868
AGTGGTA1665800.082.638545
TGGTATC1688150.082.127457
AAGCAGT1636650.081.83071
GTATCAA1702150.081.482369
GTGGTAT1695650.081.145026
GCAGTGG1707600.080.518063
AGCAGTG1767800.077.473112
CAGTGGT1776050.077.416884
CAACGCA1624700.044.335812-13
ATCAACG1637500.044.06667310-11
ACGCAGA1653900.043.66109514-15
TCAACGC1623000.042.61950312-13
AGAGTAC1690500.042.6149418-19
AACGCAG1638450.042.30805214-15
GAGTACT1639400.041.9865220-21
CGCAGAG1669450.041.51989416-17
AGTACTT1728550.041.44095220-21
TATCAAC1692050.040.9205310-11
GTACTTT1739250.039.55560322-23
Produced by FastQC (version 0.11.5)