##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660731_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 26917922 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.186603037188384 34.0 31.0 34.0 27.0 34.0 2 31.847723683871287 34.0 31.0 34.0 28.0 34.0 3 31.62264007600587 34.0 31.0 34.0 30.0 34.0 4 35.55416213034572 37.0 35.0 37.0 33.0 37.0 5 35.89214609508119 37.0 35.0 37.0 33.0 37.0 6 35.34818163155388 37.0 35.0 37.0 33.0 37.0 7 35.56938752553039 37.0 35.0 37.0 33.0 37.0 8 35.931344960431936 37.0 35.0 37.0 33.0 37.0 9 37.73631486115459 39.0 37.0 39.0 35.0 39.0 10-11 37.77160941323777 39.0 37.5 39.0 35.0 39.0 12-13 37.82776714710742 39.0 38.0 39.0 35.0 39.0 14-15 39.26910476224725 41.0 39.0 41.0 36.0 41.0 16-17 39.2371290027514 41.0 39.0 41.0 36.0 41.0 18-19 39.23730386766111 41.0 39.0 41.0 36.0 41.0 20-21 39.18407343999288 40.5 39.0 41.0 36.0 41.0 22-23 39.07547971199263 40.0 39.0 41.0 35.5 41.0 24-25 39.07831115269596 40.0 39.0 41.0 36.0 41.0 26-27 39.01874951565726 40.0 39.0 41.0 35.0 41.0 28-29 38.93294261347514 40.0 38.5 41.0 35.0 41.0 30-31 38.835819756071814 40.0 38.0 41.0 35.0 41.0 32-33 38.712837287365645 40.0 38.0 41.0 35.0 41.0 34-35 38.62538687050211 40.0 38.0 41.0 34.0 41.0 36-37 38.503310433844035 40.0 38.0 41.0 34.0 41.0 38-39 38.14450261056555 40.0 37.0 41.0 34.0 41.0 40-41 38.012348631517696 40.0 37.0 41.0 33.0 41.0 42-43 37.850555941873964 40.0 37.0 41.0 33.0 41.0 44-45 37.72573449391821 40.0 36.5 41.0 33.0 41.0 46-47 37.57725358591945 40.0 36.0 41.0 33.0 41.0 48-49 37.62298365750521 40.0 36.0 41.0 33.0 41.0 50-51 37.628332101564155 40.0 36.0 41.0 33.0 41.0 52-53 37.3955243647708 40.0 36.0 41.0 32.5 41.0 54-55 36.92796812101618 40.0 36.0 41.0 31.0 41.0 56-57 36.501507007115926 39.5 35.0 41.0 29.5 41.0 58-59 36.375348141658186 39.0 35.0 41.0 29.0 41.0 60-61 36.2293853886641 39.0 35.0 41.0 29.0 41.0 62-63 35.99461780519313 39.0 35.0 41.0 29.0 41.0 64-65 35.73183468619903 38.0 35.0 40.5 29.0 41.0 66-67 35.45009748523679 37.5 35.0 40.0 28.0 41.0 68-69 35.178191949586605 37.0 35.0 40.0 28.0 41.0 70-71 34.87146699139703 36.5 34.5 39.0 28.0 41.0 72-73 34.529010448874914 36.0 34.0 39.0 28.0 41.0 74-75 34.155160881289426 35.5 34.0 39.0 27.0 40.0 76-77 32.44269804704835 34.5 32.0 36.5 25.0 39.0 78-79 33.38839639998957 35.0 33.5 37.0 26.0 39.0 80-81 33.293713255428855 35.0 34.0 36.5 26.0 39.0 82-83 33.035768864327636 35.0 34.0 36.0 26.0 38.0 84-85 32.78506546679198 35.0 34.0 36.0 26.0 37.0 86-87 32.57054145932959 35.0 34.0 35.5 25.5 37.0 88-89 32.395703390477166 35.0 33.0 35.0 25.0 36.5 90-91 32.2322950300547 35.0 33.0 35.0 25.0 36.0 92-93 32.089330112480454 35.0 33.0 35.0 25.0 36.0 94-95 31.985231790923535 35.0 33.0 35.0 24.5 36.0 96-97 31.88445378510273 35.0 33.0 35.0 24.0 35.0 98-99 31.788645906619386 35.0 33.0 35.0 24.0 35.0 100 31.73243205771976 35.0 33.0 35.0 24.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.6348158264873227 1101 2 1.219255327009705 1101 3 1.5664910411836281 1101 4 0.9713927223086358 1101 5 0.5981126350276895 1101 6 1.131335187587048 1101 7 0.8335066148846835 1101 8 0.5133105121398529 1101 9 0.5444864461703744 1101 10-11 0.4343289141622577 1101 12-13 0.41330207013148623 1101 14-15 0.5422701266063044 1101 16-17 0.5902462595337283 1101 18-19 0.553074434359786 1101 20-21 0.546125402029368 1101 22-23 0.5479558036420116 1101 24-25 0.5402500864512305 1101 26-27 0.5255717163427107 1101 28-29 0.5362504893346554 1101 30-31 0.5173923447328477 1101 32-33 0.5750307720253005 1101 34-35 0.5951599634847824 1101 36-37 0.541528147296404 1101 38-39 0.5809757330293195 1101 40-41 0.5644291866012594 1101 42-43 0.580393994040854 1101 44-45 0.5720565027454612 1101 46-47 0.5325985510164557 1101 48-49 0.5587490067425946 1101 50-51 0.5102264943196175 1101 52-53 0.511252420841295 1101 54-55 0.5550402436881683 1101 56-57 0.5940519530242554 1101 58-59 0.5504261552347742 1101 60-61 0.5661492723836616 1101 62-63 0.5128964027650866 1101 64-65 0.5143861303896315 1101 66-67 0.5183838423932556 1101 68-69 0.5287325109409906 1101 70-71 0.5265762987785294 1101 72-73 0.4828656766585482 1101 74-75 0.48768382359292417 1101 76-77 0.40936406022569827 1101 78-79 0.5282083356429865 1101 80-81 0.5027806969332644 1101 82-83 0.5167437788614464 1101 84-85 0.5016039321192167 1101 86-87 0.5003511062922854 1101 88-89 0.4402979714712174 1101 90-91 0.43674740261951683 1101 92-93 0.46472582901515835 1101 94-95 0.44418706907519123 1101 96-97 0.4280917072235866 1101 98-99 0.408612012140086 1101 100 0.4334125626925065 1102 1 1.498684501312372 1102 2 1.12570489172667 1102 3 1.5002832926199439 1102 4 0.9153152982670107 1102 5 0.49965398312122034 1102 6 1.0623867179900444 1102 7 0.7964702069230611 1102 8 0.4644289547994731 1102 9 0.4676381777435523 1102 10-11 0.3604581615987499 1102 12-13 0.35876301204056205 1102 14-15 0.4660478592533437 1102 16-17 0.47980552859734615 1102 18-19 0.42519739254980493 1102 20-21 0.41348019693188576 1102 22-23 0.4245819976034184 1102 24-25 0.41434025834809063 1102 26-27 0.4219682872591619 1102 28-29 0.4501178097495 1102 30-31 0.4590725591620881 1102 32-33 0.4526858345446314 1102 34-35 0.4364858300400911 1102 36-37 0.4404483382726383 1102 38-39 0.46023648666806594 1102 40-41 0.4067402389350434 1102 42-43 0.4457855140078095 1102 44-45 0.3934348159683694 1102 46-47 0.41616840341697525 1102 48-49 0.3941700938503061 1102 50-51 0.38076817981990985 1102 52-53 0.3767022707409211 1102 54-55 0.4330087679760055 1102 56-57 0.4272351298536634 1102 58-59 0.4489975819236278 1102 60-61 0.42785095921737337 1102 62-63 0.3908941871940286 1102 64-65 0.37708151016209257 1102 66-67 0.34463298563729694 1102 68-69 0.3108359446368425 1102 70-71 0.3177192662683339 1102 72-73 0.2906851186971906 1102 74-75 0.3258497950200976 1102 76-77 0.1432348137653605 1102 78-79 0.3323972994401103 1102 80-81 0.3533244255186432 1102 82-83 0.35806547724985904 1102 84-85 0.3457342770428369 1102 86-87 0.3381944498883911 1102 88-89 0.3199796416351859 1102 90-91 0.37576001464327646 1102 92-93 0.36703499835269326 1102 94-95 0.3394158650757788 1102 96-97 0.317266001660073 1102 98-99 0.3397291057583267 1102 100 0.3208951133744158 1103 1 1.468353984894481 1103 2 1.0863429714649797 1103 3 1.4393967622651935 1103 4 0.8955726840132812 1103 5 0.5041677492722343 1103 6 1.0396864832931172 1103 7 0.765996747497816 1103 8 0.42945032325437893 1103 9 0.4234254387177856 1103 10-11 0.3507361526006676 1103 12-13 0.3393484929619248 1103 14-15 0.39723341430789816 1103 16-17 0.4229312450158531 1103 18-19 0.37555001273668864 1103 20-21 0.3928349854250328 1103 22-23 0.371887706316798 1103 24-25 0.38232067568679895 1103 26-27 0.4044729383939867 1103 28-29 0.3949138445783049 1103 30-31 0.3721801288979023 1103 32-33 0.4209326722465576 1103 34-35 0.4368433413766937 1103 36-37 0.3787388192425283 1103 38-39 0.38971821252762595 1103 40-41 0.40687853411331787 1103 42-43 0.4414492828740322 1103 44-45 0.4169276615903428 1103 46-47 0.43527851082968283 1103 48-49 0.36194570626925326 1103 50-51 0.3591808856515186 1103 52-53 0.3369393076852063 1103 54-55 0.33805824882555413 1103 56-57 0.35777428315413573 1103 58-59 0.3432635299119582 1103 60-61 0.3185644779801038 1103 62-63 0.30975902829742097 1103 64-65 0.31580688562946335 1103 66-67 0.3192428317793983 1103 68-69 0.308251058137472 1103 70-71 0.3309924442619945 1103 72-73 0.3206329929387479 1103 74-75 0.3317055838129548 1103 76-77 0.1855440279601197 1103 78-79 0.3237528932571223 1103 80-81 0.34817340035299793 1103 82-83 0.3008783094067198 1103 84-85 0.24328205291629956 1103 86-87 0.23180404111501218 1103 88-89 0.23025464874343982 1103 90-91 0.25233045847478763 1103 92-93 0.2521050982205679 1103 94-95 0.2213898010541655 1103 96-97 0.2724839139165951 1103 98-99 0.27194576284913907 1103 100 0.24526529497913785 1104 1 1.4571596572724381 1104 2 1.0783653992392601 1104 3 1.4239889579950962 1104 4 0.8985402532082958 1104 5 0.4791394353273404 1104 6 1.0187640501287731 1104 7 0.7628958485267248 1104 8 0.4346911878438533 1104 9 0.4417764342844066 1104 10-11 0.3233337619333483 1104 12-13 0.3144125660368857 1104 14-15 0.345454657811608 1104 16-17 0.3796641100088678 1104 18-19 0.3554574055923112 1104 20-21 0.3788813715893369 1104 22-23 0.366259591491378 1104 24-25 0.3560823658438821 1104 26-27 0.35731207341311944 1104 28-29 0.31900221775445914 1104 30-31 0.36721074980963664 1104 32-33 0.4088321269518289 1104 34-35 0.4401118564856574 1104 36-37 0.41534638681719827 1104 38-39 0.43008514562739464 1104 40-41 0.38361069054900554 1104 42-43 0.4084122631492164 1104 44-45 0.41304969397194213 1104 46-47 0.42319495099135196 1104 48-49 0.34939875690146494 1104 50-51 0.3422139238211628 1104 52-53 0.3672313878167657 1104 54-55 0.39312818817512607 1104 56-57 0.4652392780144652 1104 58-59 0.4420432961377756 1104 60-61 0.44576887132223675 1104 62-63 0.38743884937569106 1104 64-65 0.3414947580024901 1104 66-67 0.352763339761367 1104 68-69 0.311946161234097 1104 70-71 0.3359183292425527 1104 72-73 0.31094601101009545 1104 74-75 0.3412514321779341 1104 76-77 0.23941590127214596 1104 78-79 0.3704806131171807 1104 80-81 0.4117846411571051 1104 82-83 0.4095531201488427 1104 84-85 0.3667721024553856 1104 86-87 0.3838676366153351 1104 88-89 0.39314414257881936 1104 90-91 0.3786130080627288 1104 92-93 0.37044343915111 1104 94-95 0.3547477244359598 1104 96-97 0.3710293023788154 1104 98-99 0.356423434105654 1104 100 0.3403169234481602 1105 1 1.3659131628668995 1105 2 1.0035886142436894 1105 3 1.3460318783846965 1105 4 0.84115675485846 1105 5 0.4246820578822721 1105 6 0.9842934713635856 1105 7 0.7062954138491122 1105 8 0.34305032814813075 1105 9 0.331503166957674 1105 10-11 0.2592872997261537 1105 12-13 0.23338717764438854 1105 14-15 0.31239671689556303 1105 16-17 0.3047322256009224 1105 18-19 0.28285484582414 1105 20-21 0.29773005548211273 1105 22-23 0.2885490867823748 1105 24-25 0.2606194019865242 1105 26-27 0.3094370029792515 1105 28-29 0.2996297634358527 1105 30-31 0.27227783505474434 1105 32-33 0.3281332619433712 1105 34-35 0.33354419971998084 1105 36-37 0.33516520674797334 1105 38-39 0.3440108640528976 1105 40-41 0.33278789412301535 1105 42-43 0.3395350193272719 1105 44-45 0.30219779024498195 1105 46-47 0.34948853547175673 1105 48-49 0.3316422085637072 1105 50-51 0.35256350121792224 1105 52-53 0.32166336077015245 1105 54-55 0.3946768318199787 1105 56-57 0.411331880445438 1105 58-59 0.3776597494904266 1105 60-61 0.3587987996809332 1105 62-63 0.34165547981922373 1105 64-65 0.3349857022188658 1105 66-67 0.35078008289909945 1105 68-69 0.31410296715858976 1105 70-71 0.3103361027272129 1105 72-73 0.29389650605960327 1105 74-75 0.32052973854516154 1105 76-77 0.2311835580378201 1105 78-79 0.32803415090998556 1105 80-81 0.33967639033202346 1105 82-83 0.39477575558311884 1105 84-85 0.36090147539268 1105 86-87 0.3575575343855988 1105 88-89 0.3758155746000682 1105 90-91 0.4150875472239548 1105 92-93 0.3452596993137078 1105 94-95 0.33265093622055275 1105 96-97 0.35736110629090945 1105 98-99 0.3431609049259059 1105 100 0.3115037221280268 1106 1 1.3528967164423698 1106 2 0.9992944210850396 1106 3 1.3326914570300197 1106 4 0.7826913920252636 1106 5 0.3936606881020168 1106 6 0.9380235929372418 1106 7 0.6804323314659655 1106 8 0.3261772933372882 1106 9 0.29756499584841123 1106 10-11 0.21897749077047735 1106 12-13 0.18578349267874472 1106 14-15 0.24718647752381884 1106 16-17 0.25312917493264564 1106 18-19 0.21383616425652718 1106 20-21 0.22896880803063624 1106 22-23 0.24945157111371685 1106 24-25 0.24345277810976285 1106 26-27 0.18831127618018684 1106 28-29 0.22197923107913908 1106 30-31 0.248627272493394 1106 32-33 0.2806743780046901 1106 34-35 0.2712086262094502 1106 36-37 0.24171302823857133 1106 38-39 0.30686088293079195 1106 40-41 0.2855641230741668 1106 42-43 0.31057572007483714 1106 44-45 0.29433712423274727 1106 46-47 0.2726356169487545 1106 48-49 0.27309834388047705 1106 50-51 0.281222052905612 1106 52-53 0.26022604294186635 1106 54-55 0.2831929686469863 1106 56-57 0.3097110694509482 1106 58-59 0.27032370780802495 1106 60-61 0.25164084453481195 1106 62-63 0.21822640785667602 1106 64-65 0.20102376006074962 1106 66-67 0.21777079497339713 1106 68-69 0.2145406323401957 1106 70-71 0.2228014051511309 1106 72-73 0.1287787834891887 1106 74-75 0.186215731145019 1106 76-77 0.046750755802676736 1106 78-79 0.17477419470670696 1106 80-81 0.23388637296435633 1106 82-83 0.21553443564050667 1106 84-85 0.21830559832554286 1106 86-87 0.22842036002933241 1106 88-89 0.22282718828277126 1106 90-91 0.23613933307308344 1106 92-93 0.22627922661114752 1106 94-95 0.2427328209898647 1106 96-97 0.24226633924416774 1106 98-99 0.2408348760804735 1106 100 0.24198189172706108 1107 1 1.2978251714443907 1107 2 0.9171065034131871 1107 3 1.273401126441744 1107 4 0.7822894606325477 1107 5 0.3843911693354798 1107 6 0.9191868185663452 1107 7 0.6520712716205068 1107 8 0.28383116203739434 1107 9 0.2518071910769848 1107 10-11 0.16369700181464708 1107 12-13 0.11466384315329492 1107 14-15 0.16582959437938172 1107 16-17 0.18032329811607894 1107 18-19 0.1388981582496953 1107 20-21 0.13557973369292853 1107 22-23 0.15676759110443328 1107 24-25 0.18399049274750467 1107 26-27 0.14252065217972643 1107 28-29 0.14473009859406716 1107 30-31 0.12542929973965755 1107 32-33 0.15957965483976722 1107 34-35 0.17229131383277974 1107 36-37 0.1637424811912922 1107 38-39 0.1480173373232958 1107 40-41 0.19671485965759672 1107 42-43 0.23269756795097152 1107 44-45 0.18462981548711355 1107 46-47 0.21909743275415394 1107 48-49 0.20490847093226705 1107 50-51 0.2076932489298713 1107 52-53 0.199052778014142 1107 54-55 0.24626018814565498 1107 56-57 0.2128562421746878 1107 58-59 0.20003781754717664 1107 60-61 0.2256795940405354 1107 62-63 0.1894110738878183 1107 64-65 0.16078642476774263 1107 66-67 0.14397296187527076 1107 68-69 0.122354862583542 1107 70-71 0.16408440450640427 1107 72-73 0.17736481526504377 1107 74-75 0.18138482849956716 1107 76-77 0.025029454192960543 1107 78-79 0.14330987303691245 1107 80-81 0.2217956910212635 1107 82-83 0.2249207048918862 1107 84-85 0.2231304464578585 1107 86-87 0.1783879926542653 1107 88-89 0.21992445900202284 1107 90-91 0.19824487366877008 1107 92-93 0.20421826103816443 1107 94-95 0.176560454696002 1107 96-97 0.2100018207739538 1107 98-99 0.24404473267607685 1107 100 0.21793785514488562 1108 1 1.2851547269614407 1108 2 0.9096834418668323 1108 3 1.2633233951382614 1108 4 0.7554950787735848 1108 5 0.3372642362527998 1108 6 0.8788159186994235 1108 7 0.6232335586825144 1108 8 0.25831865681877275 1108 9 0.21832159095291104 1108 10-11 0.13432465925625792 1108 12-13 0.12395527110962234 1108 14-15 0.16396442220760576 1108 16-17 0.15438915473511372 1108 18-19 0.10844489265101487 1108 20-21 0.1337381475763877 1108 22-23 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-1.0522934640922657 2316 100 -0.9795430599887496 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 41.0 7 746.0 8 950.0 9 399.0 10 387.0 11 675.0 12 913.0 13 1294.0 14 2968.0 15 6762.0 16 14651.0 17 23762.0 18 31468.0 19 40964.0 20 54433.0 21 76338.0 22 115421.0 23 189814.0 24 321875.0 25 422312.0 26 424686.0 27 363407.0 28 328978.0 29 349047.0 30 407130.0 31 495075.0 32 611735.0 33 782474.0 34 1066443.0 35 1634859.0 36 2947443.0 37 5941302.0 38 7907018.0 39 2347008.0 40 5144.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 15.379508743346271 31.992936383469484 27.69123894176057 24.93631593142367 2 20.675383954931306 32.414279926490494 29.15135450728733 17.758981611290878 3 23.92428865995077 26.21777286788291 27.694513192487864 22.16342527967846 4 18.612973177440235 28.76974859308996 25.77079366841351 26.84648456105629 5 21.75180600932197 32.14265653294356 26.88717089200678 19.218366565727685 6 27.68110461655843 27.79124541696123 26.19317280851945 18.334477157960894 7 20.773881206486244 26.729324517829863 32.18708456268659 20.3097097129973 8 25.456040305743578 26.82937430629213 27.068613315379448 20.645972072584843 9 24.90423758952261 25.18981685101164 29.003803822739506 20.90214173672625 10-11 20.45061937581623 29.62978435246783 29.44399352609375 20.47560274562219 12-13 20.40948853367985 27.077860136375314 29.1841204972702 23.32853083267463 14-15 20.37438848362812 32.45734013197602 26.553981395740728 20.614289988655145 16-17 23.259892589375745 26.77881676160313 26.6088593156056 23.35243133341552 18-19 20.43323960891186 29.586180538007355 26.66032318542271 23.320256667658075 20-21 23.1836333017929 29.718439413578285 26.596421405440186 20.501505879188628 22-23 23.155215382233212 26.991965424361297 29.315882673217747 20.536936520187744 24-25 20.5216707289129 29.526427584083443 26.46556455018734 23.486337136816317 26-27 20.579969612059177 26.809608934749328 32.04025236535634 20.570169087835154 28-29 20.5775277874931 26.915636749641834 32.01897099617829 20.487864466686773 30-31 20.519150845470715 26.820432668243686 32.0247455858365 20.6356709004491 32-33 20.68817977139333 26.99192976653987 31.769205304724647 20.550685157342162 34-35 20.668555196377287 27.112746249163276 31.651210429817144 20.567488124642292 36-37 20.594266352857257 27.02747751847841 31.763716003722614 20.61454012494172 38-39 20.634191896972066 27.000792747766 31.802972495761523 20.562042859500412 40-41 20.66158153710425 27.03452722957532 31.76075928559013 20.5431319477303 42-43 20.652277225809737 27.081755551914167 31.705087060836128 20.56088016143997 44-45 20.71089639573877 27.144763396467138 31.662556580232682 20.481783627561416 46-47 20.728943242487794 27.24651677305678 31.547896492198625 20.476643492256798 48-49 20.75126206668053 27.277496348103885 31.438506083210644 20.532735502004943 50-51 20.768950833614362 27.36718411511291 31.35505565295586 20.50880939831687 52-53 20.89206357461126 27.467286276950254 31.07829478528485 20.56235536315364 54-55 21.14411231498129 27.677918483894693 30.45687146734151 20.721097733782504 56-57 21.560080596091517 28.17183985939345 29.407889982061892 20.860189562453147 58-59 21.658747364737888 28.66916416261264 28.76119467370793 20.910893798941544 60-61 21.71065284399301 28.92226997761237 28.41939546412309 20.947681714271532 62-63 21.70986358775106 29.07125086854801 28.23576332805711 20.983122215643817 64-65 21.729350478925433 29.1484177689533 28.13280832951417 20.9894234226071 66-67 21.667219596784975 29.044398863741616 28.258367134795172 21.030014404678244 68-69 21.623651126625234 28.93282293685056 28.38897596971296 21.054549966811244 70-71 21.563173683701457 28.894040020171342 28.436582835759477 21.106203460367727 72-73 21.540165288266902 28.829545259585686 28.396827010415944 21.23346244173147 74-75 21.605288307667948 28.901393540031794 28.23654867167819 21.256769480622065 76-77 21.659545117969277 29.00237603858758 28.025440191629446 21.3126386518137 78-79 21.749278638203183 29.15902568723932 27.687235195493752 21.40446047906374 80-81 21.831997779071347 29.29945222767671 27.373969676919778 21.494580316332172 82-83 21.84021411896647 29.41875989568201 27.059666843870122 21.681359141481394 84-85 21.846803065061188 29.430365232310407 26.91583421670411 21.8069974859243 86-87 21.858636792442486 29.405451686837182 26.833146677047903 21.902764843672433 88-89 21.902021517318353 29.390375731435537 26.702206256620087 22.005396494626023 90-91 21.93525053643904 29.334129020184243 26.586769749598215 22.143850693778504 92-93 21.927334996410945 29.304474445548536 26.567920403496903 22.20027015454362 94-95 21.987015196635458 29.23508017117594 26.510044439814656 22.26786019237394 96-97 22.05384388019511 29.103935592723616 26.487745958164172 22.354474568917098 98-99 22.10776761487089 29.035418637290018 26.471743549309906 22.38507019852919 100 22.066122825965405 28.9550998905111 26.418059612936112 22.560717670587387 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 16790.0 1 13593.5 2 8535.5 3 6227.0 4 5772.5 5 5835.0 6 6035.0 7 6196.5 8 6373.5 9 6706.0 10 7499.5 11 14032.0 12 98548.0 13 116057.5 14 53904.5 15 56577.0 16 64872.0 17 73435.0 18 81126.5 19 88811.5 20 99321.5 21 115760.0 22 139011.0 23 167070.5 24 196074.0 25 227462.5 26 264936.0 27 310176.5 28 364830.0 29 420564.5 30 469385.0 31 514286.0 32 559877.0 33 599103.5 34 632882.5 35 671418.5 36 714626.0 37 757959.5 38 801745.5 39 831143.5 40 841655.0 41 862617.0 42 896333.0 43 905305.0 44 901484.5 45 909392.5 46 911918.5 47 907367.0 48 896725.0 49 883349.0 50 872197.5 51 860386.0 52 840614.5 53 800912.5 54 745922.5 55 691483.0 56 635458.5 57 571854.0 58 503720.5 59 429758.5 60 352092.5 61 283478.0 62 222120.5 63 165310.0 64 120378.0 65 86691.5 66 61566.5 67 44506.0 68 32558.5 69 23804.5 70 17535.5 71 13196.0 72 10506.5 73 8202.5 74 6305.0 75 5201.5 76 3927.5 77 2337.0 78 1284.5 79 776.5 80 486.0 81 328.5 82 208.5 83 143.5 84 119.5 85 82.0 86 49.0 87 34.0 88 23.0 89 15.0 90 14.0 91 9.5 92 2.5 93 2.5 94 2.0 95 1.5 96 1.0 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.32917102590608593 2 0.006230050001630883 3 2.3941186841985798 4 9.101742697671834E-4 5 3.714997019457891E-5 6 0.008819402924193035 7 0.007459714015071446 8 2.637647883815103E-4 9 3.7149970194578915E-6 10-11 5.9439952311326264E-5 12-13 3.7149970194578915E-6 14-15 0.0 16-17 2.7862477645934183E-5 18-19 0.0 20-21 3.41779725790126E-4 22-23 3.714997019457891E-5 24-25 5.12669588685189E-4 26-27 0.0036945645358508732 28-29 0.006226335004611425 30-31 0.005667227953183013 32-33 0.015381945159065397 34-35 0.009761154668625609 36-37 0.005096975910696227 38-39 0.005260435779552374 40-41 0.0083680307863289 42-43 0.019065364703857897 44-45 0.033882630315965695 46-47 0.032909301096867724 48-49 0.03962787320655733 50-51 0.039512708298954126 52-53 0.06208317269067055 54-55 0.03313963091207412 56-57 0.02209308727471608 58-59 0.016958961393825273 60-61 0.01800287555629294 62-63 0.024626715241986362 64-65 0.04442764935569692 66-67 0.04745537192655511 68-69 0.05007258732676319 70-71 0.06088137115487592 72-73 0.06847668256115758 74-75 0.05918733251400313 76-77 0.05944738230536518 78-79 0.05374300438198759 80-81 0.051493573686705825 82-83 0.04784544661359818 84-85 0.04978839005477466 86-87 0.05770504870323943 88-89 0.06431217090234528 90-91 0.0602442491660389 92-93 0.06461680065794084 94-95 0.07293467898450705 96-97 0.07233284946735487 98-99 0.04768384424325176 100 0.007251674181981804 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2.6917922E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 39.925645027675735 #Duplication Level Percentage of deduplicated Percentage of total 1 67.71705883177101 27.036472532355234 2 16.81501453821464 13.427006031759289 3 5.843726444062903 6.999436429334917 4 2.7236155641357094 4.349684328221405 5 1.5140356830427826 3.0224425620200353 6 1.050227886851517 2.5158615485159794 7 0.7550869190610238 2.110313260683023 8 0.5329162414395423 1.702161974815864 9 0.38030540298792637 1.3665544669622804 >10 2.320445904952069 16.943028400764078 >50 0.20545578897562222 5.785703780700172 >100 0.14068133517924042 9.215999083752232 >500 8.767652757893116E-4 0.21822612900041644 >1k 4.8731345801272374E-4 0.3426366429614964 >5k 2.8020253841553157E-5 0.07716603925854149 >10k+ 3.7360338450911375E-5 4.887306788894967 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 1226133 4.555080440458963 No Hit AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 41338 0.15357054679035032 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 5.423895648408522E-4 1.1144991058373673E-5 0.0 0.0 4.08649672140368E-5 2 5.498195588797679E-4 2.2289982116747346E-5 0.0 0.0 4.08649672140368E-5 3 5.572495529186837E-4 2.2289982116747346E-5 0.0 0.0 4.08649672140368E-5 4 5.758245380159732E-4 3.343497317512102E-5 0.0 7.429994038915783E-6 4.08649672140368E-5 5 5.758245380159732E-4 5.5724955291868366E-5 0.0 7.429994038915783E-6 4.457996423349469E-5 6 5.758245380159732E-4 7.429994038915782E-5 0.0 7.429994038915783E-6 4.457996423349469E-5 7 5.869695290743468E-4 8.17299344280736E-5 0.0 7.429994038915783E-6 4.457996423349469E-5 8 5.943995231132626E-4 9.287492548644728E-5 0.0 7.429994038915783E-6 4.457996423349469E-5 9 6.278344962883836E-4 9.658992250590517E-5 0.0 7.429994038915783E-6 4.457996423349469E-5 10-11 6.594119709537757E-4 1.1330740909346568E-4 0.0 7.429994038915783E-6 5.2009958272410475E-5 12-13 6.85416950089981E-4 1.263098986615683E-4 0.0 9.287492548644728E-6 5.2009958272410475E-5 14-15 7.207094217748309E-4 1.4116988673939986E-4 0.0 1.4859988077831566E-5 5.2009958272410475E-5 16-17 7.690043830277835E-4 1.5602987481723143E-4 0.0 1.4859988077831566E-5 5.2009958272410475E-5 18-19 8.674518040434176E-4 1.7831985693397877E-4 3.7149970194578915E-6 1.671748658756051E-5 5.2009958272410475E-5 20-21 9.343217503936596E-4 1.9689484203126823E-4 3.7149970194578915E-6 1.8574985097289455E-5 5.5724955291868366E-5 22-23 0.0010123366878022754 2.0060983905072612E-4 3.7149970194578915E-6 1.8574985097289455E-5 5.9439952311326264E-5 24-25 0.0013039639538297198 2.080398330896419E-4 3.7149970194578915E-6 1.8574985097289455E-5 6.686994635024204E-5 26-27 0.00236088060586549 2.451898032842208E-4 3.7149970194578915E-6 1.8574985097289455E-5 6.686994635024204E-5 28-29 0.005594785511303584 2.6376478838151024E-4 3.7149970194578915E-6 1.8574985097289455E-5 6.686994635024204E-5 30-31 0.013658186542036937 2.6747978540096816E-4 3.7149970194578915E-6 1.8574985097289455E-5 7.801493740861571E-5 32-33 0.030409108102772565 2.8605477049825764E-4 3.7149970194578915E-6 1.8574985097289455E-5 8.730242995726044E-5 34-35 0.06136060577038599 3.0648725410527603E-4 3.7149970194578915E-6 1.8574985097289455E-5 8.915992846698939E-5 36-37 0.11122515326405953 3.1577474665392077E-4 3.7149970194578915E-6 1.8574985097289455E-5 8.915992846698939E-5 38-39 0.18402237735884663 3.1948974367337863E-4 3.7149970194578915E-6 1.8574985097289455E-5 9.101742697671833E-5 40-41 0.28234720347283865 3.269197377122944E-4 3.7149970194578915E-6 2.2289982116747346E-5 9.287492548644728E-5 42-43 0.4091140467678003 3.3434973175121025E-4 3.7149970194578915E-6 2.2289982116747346E-5 9.287492548644728E-5 44-45 0.5710897743146741 3.473522213193128E-4 3.7149970194578915E-6 2.2289982116747346E-5 1.0401991654482095E-4 46-47 0.7735069594153665 3.696422034360602E-4 3.7149970194578915E-6 2.2289982116747346E-5 1.0773491356427884E-4 48-49 1.0229281443047498 3.9378968406253645E-4 3.7149970194578915E-6 2.6004979136205238E-5 1.263098986615683E-4 50-51 1.311390232871616 4.1793716468901276E-4 3.7149970194578915E-6 3.343497317512102E-5 1.3373989270048408E-4 52-53 1.634119453945962 4.3093965425711536E-4 3.7149970194578915E-6 3.343497317512102E-5 1.393123882296709E-4 54-55 2.0022180761204376 4.5322963637386277E-4 3.7149970194578915E-6 3.343497317512102E-5 1.4859988077831564E-4 56-57 2.421856709444362 4.959521020976284E-4 3.7149970194578915E-6 3.343497317512102E-5 1.504573792880446E-4 58-59 2.899269118916386 5.219570812338338E-4 3.7149970194578915E-6 3.5292471684849965E-5 1.5788737332696038E-4 60-61 3.4239492929654824 5.4610456186031E-4 3.7149970194578915E-6 4.08649672140368E-5 1.634598688561472E-4 62-63 3.984575778174853 5.721095409965153E-4 3.7149970194578915E-6 4.457996423349469E-5 1.6903236438533403E-4 64-65 4.582864903167488 5.999720186424494E-4 3.7149970194578915E-6 4.457996423349469E-5 1.7274736140479195E-4 66-67 5.225869589784828 6.074020126813652E-4 3.7149970194578915E-6 4.457996423349469E-5 1.7831985693397877E-4 68-69 5.917696767231884 6.148320067202811E-4 3.7149970194578915E-6 4.8294961252952584E-5 1.838923524631656E-4 70-71 6.6529448298423635 6.241194992689257E-4 3.7149970194578915E-6 5.2009958272410475E-5 1.9317984501181034E-4 72-73 7.417290606607747 6.575544724440469E-4 3.7149970194578915E-6 5.2009958272410475E-5 2.1546982712855768E-4 74-75 8.210830687450539 6.83559451580252E-4 3.7149970194578915E-6 5.2009958272410475E-5 2.2475731967720242E-4 76-77 9.037274496894671 7.039919351872705E-4 3.7149970194578915E-6 5.5724955291868366E-5 2.2661481818693136E-4 78-79 9.894608506555596 7.318544128332046E-4 3.7149970194578915E-6 5.758245380159731E-5 2.2661481818693136E-4 80-81 10.7777134505405 7.448569024013072E-4 3.7149970194578915E-6 5.9439952311326264E-5 2.284723166966603E-4 82-83 11.684185725777791 7.727193800472413E-4 3.7149970194578915E-6 5.9439952311326264E-5 2.3032981520638925E-4 84-85 12.613495945192202 7.912943651445308E-4 3.7149970194578915E-6 5.9439952311326264E-5 2.3032981520638925E-4 86-87 13.558684061867776 8.005818576931756E-4 3.7149970194578915E-6 5.9439952311326264E-5 2.4147480626476295E-4 88 14.277955779796077 8.061543532223624E-4 3.7149970194578915E-6 5.9439952311326264E-5 2.4890480030367873E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 166840 0.0 83.184586 8 AGTGGTA 166580 0.0 82.63854 5 TGGTATC 168815 0.0 82.12745 7 AAGCAGT 163665 0.0 81.8307 1 GTATCAA 170215 0.0 81.48236 9 GTGGTAT 169565 0.0 81.14502 6 GCAGTGG 170760 0.0 80.51806 3 AGCAGTG 176780 0.0 77.47311 2 CAGTGGT 177605 0.0 77.41688 4 CAACGCA 162470 0.0 44.3358 12-13 ATCAACG 163750 0.0 44.066673 10-11 ACGCAGA 165390 0.0 43.661095 14-15 TCAACGC 162300 0.0 42.619503 12-13 AGAGTAC 169050 0.0 42.61494 18-19 AACGCAG 163845 0.0 42.308052 14-15 GAGTACT 163940 0.0 41.98652 20-21 CGCAGAG 166945 0.0 41.519894 16-17 AGTACTT 172855 0.0 41.440952 20-21 TATCAAC 169205 0.0 40.92053 10-11 GTACTTT 173925 0.0 39.555603 22-23 >>END_MODULE