FastQCFastQC Report
Thu 26 May 2016
SRR1660730_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660730_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29346392
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT14769015.032649328748829No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT434320.14799775045600155No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1903350.084.560048
AGTGGTA1924800.083.7832265
TGGTATC1926700.083.351147
GTATCAA1938600.082.905279
GTGGTAT1960400.082.2956856
AAGCAGT1979150.081.9268951
GCAGTGG2002600.080.949293
CAGTGGT2036550.079.106984
AGCAGTG2088600.077.672
CAACGCA1873650.044.6031812-13
ATCAACG1888150.044.34551210-11
ACGCAGA1899100.044.03523614-15
AGAGTAC1905450.043.23201818-19
TCAACGC1871550.043.1009512-13
AACGCAG1889650.042.7987314-15
GAGTACT1857700.042.52712620-21
AGTACTT1936850.042.1398220-21
CGCAGAG1919350.042.0498216-17
TATCAAC1939300.041.42111610-11
GTACTTT1957750.040.2987822-23
Produced by FastQC (version 0.11.5)