##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660730_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 29346392 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.893127134674682 34.0 31.0 34.0 30.0 34.0 2 32.019164604630106 34.0 31.0 34.0 30.0 34.0 3 32.074408022628475 34.0 31.0 34.0 30.0 34.0 4 35.484286961068335 37.0 35.0 37.0 33.0 37.0 5 35.453329935754965 37.0 35.0 37.0 33.0 37.0 6 35.522515510594964 37.0 35.0 37.0 33.0 37.0 7 35.483327320101225 37.0 35.0 37.0 33.0 37.0 8 35.4890046790079 37.0 35.0 37.0 33.0 37.0 9 37.17264636824861 39.0 37.0 39.0 34.0 39.0 10-11 37.11755436579733 39.0 37.0 39.0 34.0 39.0 12-13 37.13198053443844 39.0 37.0 39.0 33.5 39.0 14-15 38.50836084040587 40.0 38.0 41.0 34.0 41.0 16-17 38.48847337008243 40.0 38.0 41.0 34.0 41.0 18-19 38.45775461937535 40.0 38.0 41.0 34.0 41.0 20-21 38.34304114795441 40.0 38.0 41.0 33.5 41.0 22-23 38.17528504015077 40.0 38.0 41.0 33.5 41.0 24-25 38.15657519329804 40.0 38.0 41.0 33.0 41.0 26-27 38.13403047979459 40.0 38.0 41.0 33.0 41.0 28-29 38.047897932393184 40.0 38.0 41.0 33.0 41.0 30-31 37.955822116054335 40.0 38.0 41.0 33.0 41.0 32-33 37.82886535080701 40.0 38.0 41.0 32.0 41.0 34-35 37.6731508254916 40.0 38.0 41.0 32.0 41.0 36-37 37.486796025896474 40.0 37.0 41.0 31.0 41.0 38-39 37.08599520513459 40.0 35.5 41.0 31.0 41.0 40-41 37.02595334377051 40.0 35.0 41.0 31.0 41.0 42-43 37.036430883905595 40.0 35.0 41.0 31.0 41.0 44-45 37.039704932040706 40.0 35.0 41.0 31.0 41.0 46-47 37.134598982389385 40.0 36.0 41.0 31.0 41.0 48-49 37.03125488134964 40.0 35.0 41.0 31.0 41.0 50-51 35.66933134403712 38.5 34.0 40.0 28.5 40.5 52-53 35.83625082429213 38.5 35.0 39.5 28.5 40.5 54-55 36.26005682402116 39.5 35.0 41.0 27.5 41.0 56-57 35.921767912730125 39.5 35.0 41.0 26.0 41.0 58-59 35.736547477454806 39.0 35.0 41.0 26.0 41.0 60-61 35.51315662586392 39.0 35.0 41.0 26.0 41.0 62-63 35.286103654582135 39.0 35.0 41.0 25.0 41.0 64-65 35.03352158248278 38.0 35.0 40.5 25.0 41.0 66-67 34.76494282499873 37.0 34.0 40.0 25.0 41.0 68-69 34.482457110911625 37.0 34.0 40.0 24.5 41.0 70-71 34.17321957329541 36.0 34.0 39.0 24.0 41.0 72-73 33.82571835406547 36.0 34.0 39.0 24.0 41.0 74-75 33.44769087797914 35.0 33.0 38.5 23.5 40.5 76-77 33.05197889403236 35.0 33.0 37.0 22.5 39.5 78-79 32.68085015357254 35.0 33.0 37.0 21.5 39.0 80-81 32.36080278625052 35.0 33.0 36.5 20.5 39.0 82-83 32.048271777327855 35.0 32.5 36.0 20.0 38.0 84-85 31.75731307957721 35.0 32.0 36.0 20.0 37.0 86-87 31.53959072720081 35.0 32.0 35.0 19.5 37.0 88-89 31.330080764272488 35.0 32.0 35.0 19.0 36.0 90-91 31.156117811688745 35.0 32.0 35.0 18.5 36.0 92-93 31.001012884309596 35.0 31.5 35.0 18.0 36.0 94-95 30.818871260221698 35.0 31.0 35.0 18.0 35.5 96-97 30.683897206171036 35.0 31.0 35.0 17.0 35.0 98-99 30.578077076732292 35.0 31.0 35.0 17.0 35.0 100 30.497665880016868 34.0 31.0 35.0 17.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.479257634020275 1101 2 0.5003576336037909 1101 3 0.505985045848 1101 4 0.4123961918029124 1101 5 0.3988198349795198 1101 6 0.4063011809901269 1101 7 0.4189563219974488 1101 8 0.4282960640021045 1101 9 0.5114399865390453 1101 10-11 0.5253473985432962 1101 12-13 0.5175889252778845 1101 14-15 0.6436178617561339 1101 16-17 0.6031514160156703 1101 18-19 0.574465774799954 1101 20-21 0.6212012177924464 1101 22-23 0.6571788286931692 1101 24-25 0.6718252160922376 1101 26-27 0.6441954232884441 1101 28-29 0.6443874408305774 1101 30-31 0.6150583651583261 1101 32-33 0.5796096854249342 1101 34-35 0.5977139337457089 1101 36-37 0.564614338655332 1101 38-39 0.6076631052915147 1101 40-41 0.5930282015231754 1101 42-43 0.6179563683362161 1101 44-45 0.5817442554229899 1101 46-47 0.6233607500195433 1101 48-49 0.6665191812335252 1101 50-51 0.5853334022214582 1101 52-53 0.6178918511509508 1101 54-55 0.7053039718441454 1101 56-57 0.7173660213002577 1101 58-59 0.6222887066752065 1101 60-61 0.6194829323850612 1101 62-63 0.5883835703308122 1101 64-65 0.5585506383001544 1101 66-67 0.5323429775814859 1101 68-69 0.5279266055176635 1101 70-71 0.5102801506900931 1101 72-73 0.5061273108528255 1101 74-75 0.4664059722148082 1101 76-77 0.4667462070533901 1101 78-79 0.44624266778421173 1101 80-81 0.3681945872531358 1101 82-83 0.37414522195952316 1101 84-85 0.4015463554479801 1101 86-87 0.3708087326248233 1101 88-89 0.32674968911004143 1101 90-91 0.32970873362116393 1101 92-93 0.32725398336158307 1101 94-95 0.36101682026112414 1101 96-97 0.3587761920129644 1101 98-99 0.29427147807466625 1101 100 0.2768987538353258 1102 1 0.42201419881943636 1102 2 0.45748385916660794 1102 3 0.43877151321994745 1102 4 0.3674929841533725 1102 5 0.40548411932710593 1102 6 0.3474683459775747 1102 7 0.370935347953548 1102 8 0.4022488399904063 1102 9 0.44967525731274804 1102 10-11 0.4263868510992168 1102 12-13 0.44544073328481204 1102 14-15 0.555511247148992 1102 16-17 0.5311947515141853 1102 18-19 0.5010825185279799 1102 20-21 0.49789695967552206 1102 22-23 0.5477978263208456 1102 24-25 0.5293704057045332 1102 26-27 0.5011187669277959 1102 28-29 0.5028183765386629 1102 30-31 0.4735606198222513 1102 32-33 0.47843867405853757 1102 34-35 0.4988968124712372 1102 36-37 0.5161049101086945 1102 38-39 0.5672890038951763 1102 40-41 0.48232012614392517 1102 42-43 0.5024402553175165 1102 44-45 0.5161358669796599 1102 46-47 0.5478213172931845 1102 48-49 0.5799929134635846 1102 50-51 0.5565505074583186 1102 52-53 0.5904439071774021 1102 54-55 0.646883376545226 1102 56-57 0.6616446370251765 1102 58-59 0.5666665981308299 1102 60-61 0.5818433713175253 1102 62-63 0.5919841998729325 1102 64-65 0.5746388980286739 1102 66-67 0.5458886050111076 1102 68-69 0.5398049634844782 1102 70-71 0.5655902529929335 1102 72-73 0.500826864629758 1102 74-75 0.5161153755704433 1102 76-77 0.49024011811098944 1102 78-79 0.495210644999041 1102 80-81 0.4627457812931439 1102 82-83 0.45599616562643064 1102 84-85 0.408093427599308 1102 86-87 0.3934734075262405 1102 88-89 0.37239698187189774 1102 90-91 0.3680261485464982 1102 92-93 0.3403988671720093 1102 94-95 0.3711253057225399 1102 96-97 0.3791443997342334 1102 98-99 0.3132025924470945 1102 100 0.3005575467080597 1103 1 0.38492701653153816 1103 2 0.4038129995511781 1103 3 0.40488529738540535 1103 4 0.3586870724366662 1103 5 0.38861069797031433 1103 6 0.3577631778274224 1103 7 0.35457262725229555 1103 8 0.3751827120650404 1103 9 0.43816847998642316 1103 10-11 0.4349676014590287 1103 12-13 0.45593542385868346 1103 14-15 0.5310819434373215 1103 16-17 0.49066065387531665 1103 18-19 0.49420330154809733 1103 20-21 0.4986201109721904 1103 22-23 0.5275470172803338 1103 24-25 0.5622862360916443 1103 26-27 0.5103889572326707 1103 28-29 0.5271998556160895 1103 30-31 0.512700897152591 1103 32-33 0.4540806866766758 1103 34-35 0.459332168452697 1103 36-37 0.4449732179487711 1103 38-39 0.470038625520651 1103 40-41 0.4276099607720383 1103 42-43 0.4036180167721568 1103 44-45 0.42632612362255173 1103 46-47 0.4873665491626724 1103 48-49 0.5185710439265137 1103 50-51 0.456678482885863 1103 52-53 0.43804405186943285 1103 54-55 0.508779139789759 1103 56-57 0.5325389150813393 1103 58-59 0.4567805312876416 1103 60-61 0.4712234256572927 1103 62-63 0.4570142901963692 1103 64-65 0.43536135655086383 1103 66-67 0.4016967543691763 1103 68-69 0.3855969262557295 1103 70-71 0.3696587852341935 1103 72-73 0.3658599353149583 1103 74-75 0.3591061821478476 1103 76-77 0.3785721104894293 1103 78-79 0.3611240190661178 1103 80-81 0.29338667926786144 1103 82-83 0.32109908028034795 1103 84-85 0.3306900511728479 1103 86-87 0.2815897550641715 1103 88-89 0.25924981788332957 1103 90-91 0.28175968111590066 1103 92-93 0.2716396060913375 1103 94-95 0.3117477111687492 1103 96-97 0.27681371480650085 1103 98-99 0.245499234782816 1103 100 0.2639317368957279 1104 1 0.37500558693338704 1104 2 0.3968525002622556 1104 3 0.39376016042414363 1104 4 0.336565949779029 1104 5 0.35541507507608117 1104 6 0.2876975461241358 1104 7 0.2961024121638687 1104 8 0.31746353121365445 1104 9 0.40589059193479926 1104 10-11 0.32788453574372767 1104 12-13 0.3749424702426225 1104 14-15 0.46044706004681046 1104 16-17 0.4186339042555858 1104 18-19 0.4157971663811182 1104 20-21 0.36267131074866654 1104 22-23 0.3996800037359236 1104 24-25 0.4015526330560917 1104 26-27 0.38705218983840695 1104 28-29 0.3969471387617247 1104 30-31 0.35568798172621996 1104 32-33 0.36032482011523825 1104 34-35 0.3682695482634486 1104 36-37 0.3277937446045982 1104 38-39 0.30553148530520247 1104 40-41 0.32727170008676154 1104 42-43 0.32621675780989534 1104 44-45 0.31960744733213886 1104 46-47 0.36556208247603905 1104 48-49 0.37868357506457784 1104 50-51 0.3209444542627651 1104 52-53 0.28938525243194846 1104 54-55 0.34380232735497884 1104 56-57 0.3566751559339991 1104 58-59 0.32950280987943614 1104 60-61 0.3568777133645611 1104 62-63 0.30155294878804995 1104 64-65 0.3165957956244796 1104 66-67 0.2935883465869651 1104 68-69 0.27569376222687936 1104 70-71 0.2542523079024903 1104 72-73 0.26636861583306626 1104 74-75 0.21513226446860045 1104 76-77 0.24673607263348174 1104 78-79 0.2507516361270845 1104 80-81 0.22555477165806792 1104 82-83 0.2111954210984237 1104 84-85 0.19970609365129377 1104 86-87 0.16253831716091227 1104 88-89 0.16896138696268181 1104 90-91 0.16214465998203664 1104 92-93 0.19129517468289947 1104 94-95 0.22768721939150183 1104 96-97 0.20376190835592567 1104 98-99 0.15440557058791526 1104 100 0.10825448712710539 1105 1 0.26400348398020057 1105 2 0.2991475042546412 1105 3 0.2825001087179828 1105 4 0.24651512591655944 1105 5 0.2508640323484954 1105 6 0.22636490275877463 1105 7 0.24080830832844669 1105 8 0.24531556097009855 1105 9 0.28993325529053493 1105 10-11 0.28566218885139705 1105 12-13 0.2735019297156711 1105 14-15 0.33832431741491575 1105 16-17 0.2875295794080017 1105 18-19 0.30402568293145293 1105 20-21 0.3260519698734967 1105 22-23 0.37888251392849526 1105 24-25 0.3942258591758403 1105 26-27 0.4059179940385391 1105 28-29 0.38644329717014614 1105 30-31 0.3626182991593865 1105 32-33 0.36158324106175854 1105 34-35 0.35643580392979146 1105 36-37 0.35493446521884664 1105 38-39 0.39052088043477795 1105 40-41 0.3715520531555896 1105 42-43 0.41428185968172926 1105 44-45 0.42200895877240185 1105 46-47 0.4204329944824039 1105 48-49 0.4402155071898548 1105 50-51 0.33290642648683644 1105 52-53 0.36055790739118976 1105 54-55 0.46231878265373183 1105 56-57 0.4640950202438816 1105 58-59 0.44898007824424013 1105 60-61 0.4025201469998976 1105 62-63 0.4141421403077956 1105 64-65 0.4050706032489373 1105 66-67 0.3772199986615803 1105 68-69 0.3415740809113146 1105 70-71 0.3524346011806827 1105 72-73 0.35957907843705783 1105 74-75 0.34104692282742377 1105 76-77 0.3644735319060146 1105 78-79 0.35793004672408557 1105 80-81 0.2665242584317937 1105 82-83 0.29770399758205457 1105 84-85 0.28826554687622874 1105 86-87 0.2603254003277833 1105 88-89 0.27089660440272567 1105 90-91 0.24926687103035405 1105 92-93 0.2533022895829866 1105 94-95 0.3039103425635794 1105 96-97 0.3142919460179563 1105 98-99 0.2619242801759327 1105 100 0.25677478896660944 1106 1 0.2281519281121227 1106 2 0.28559478487978396 1106 3 0.25397142975784703 1106 4 0.24980809925633451 1106 5 0.243823835823882 1106 6 0.22450771468997033 1106 7 0.2169516348666818 1106 8 0.2332060315418687 1106 9 0.2675817604421269 1106 10-11 0.23082178847139545 1106 12-13 0.24073709217816486 1106 14-15 0.31989532496669426 1106 16-17 0.2874360698861551 1106 18-19 0.2470386088674701 1106 20-21 0.2685953657099063 1106 22-23 0.2657644320813617 1106 24-25 0.2628791486550952 1106 26-27 0.2847397219054457 1106 28-29 0.269318177754144 1106 30-31 0.23883177483354956 1106 32-33 0.317954269224515 1106 34-35 0.3089432649037107 1106 36-37 0.24136934230159568 1106 38-39 0.25458297808904007 1106 40-41 0.2730905311799461 1106 42-43 0.30742017566097957 1106 44-45 0.33654003343123406 1106 46-47 0.3083676242853315 1106 48-49 0.3443544328961252 1106 50-51 0.2284117834254502 1106 52-53 0.2885558202886287 1106 54-55 0.4158640600267489 1106 56-57 0.4534280590711006 1106 58-59 0.3785532855198639 1106 60-61 0.40665431052701706 1106 62-63 0.39122916686370246 1106 64-65 0.3237823372608304 1106 66-67 0.33434109908512966 1106 68-69 0.3431528668950321 1106 70-71 0.37040218815546666 1106 72-73 0.32454978578245175 1106 74-75 0.30798843014153476 1106 76-77 0.2910830959289399 1106 78-79 0.33248460808462 1106 80-81 0.2838167987877753 1106 82-83 0.310122323984956 1106 84-85 0.2902640485634116 1106 86-87 0.20982939088064612 1106 88-89 0.22983136091694334 1106 90-91 0.24354530003478914 1106 92-93 0.20574036833823683 1106 94-95 0.23907360595924843 1106 96-97 0.2622293520489656 1106 98-99 0.2685613526121493 1106 100 0.26194571584932547 1107 1 0.19807929642236743 1107 2 0.22303313346093745 1107 3 0.2216267617521126 1107 4 0.2027766290553643 1107 5 0.22037924586449265 1107 6 0.17633470989600397 1107 7 0.18223052271049056 1107 8 0.2194871915190575 1107 9 0.21899956420158162 1107 10-11 0.2137855673418798 1107 12-13 0.21843738780916055 1107 14-15 0.24261472488801417 1107 16-17 0.23967296602579324 1107 18-19 0.21613870836389282 1107 20-21 0.19707801662116964 1107 22-23 0.22385540048428965 1107 24-25 0.2556552510574406 1107 26-27 0.24814340778810617 1107 28-29 0.2803335856124036 1107 30-31 0.2316866742061947 1107 32-33 0.18335928445014815 1107 34-35 0.24164787208972172 1107 36-37 0.2154151441776122 1107 38-39 0.20301714159338502 1107 40-41 0.23130600415755964 1107 42-43 0.2892088013460423 1107 44-45 0.29806057202239344 1107 46-47 0.2737762072832268 1107 48-49 0.30962936194094937 1107 50-51 0.21955281402844662 1107 52-53 0.20154290925159302 1107 54-55 0.3295284340935254 1107 56-57 0.31827685726199917 1107 58-59 0.281251370417003 1107 60-61 0.2880621501304077 1107 62-63 0.24344340147907673 1107 64-65 0.262206442966054 1107 66-67 0.30210262534213683 1107 68-69 0.27276265004362443 1107 70-71 0.25865600218211426 1107 72-73 0.2747208983398295 1107 74-75 0.2627818969408864 1107 76-77 0.27315780666067724 1107 78-79 0.2678258262362334 1107 80-81 0.18286563135205114 1107 82-83 0.24348533282674367 1107 84-85 0.24862229714008421 1107 86-87 0.17089099073572456 1107 88-89 0.19644997982754475 1107 90-91 0.2319271401877181 1107 92-93 0.22333927739399684 1107 94-95 0.24719333377727892 1107 96-97 0.25214980843021806 1107 98-99 0.25269150274385765 1107 100 0.227493427636702 1108 1 0.1297595578086792 1108 2 0.12423344638482092 1108 3 0.15122028366657503 1108 4 0.1355719804685549 1108 5 0.14634205800125955 1108 6 0.09276365566897482 1108 7 0.11004687775815825 1108 8 0.11377729624933153 1108 9 0.13044912205937464 1108 10-11 0.10379762876321053 1108 12-13 0.09556502415419033 1108 14-15 0.1429813244765512 1108 16-17 0.127952851370857 1108 18-19 0.11897126434787708 1108 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-1.1068230613261036 2316 82-83 -1.063446211395572 2316 84-85 -1.181165987060286 2316 86-87 -1.3895065147428625 2316 88-89 -1.3335035651869127 2316 90-91 -1.2349807873757506 2316 92-93 -1.112273115847774 2316 94-95 -1.2878777261503238 2316 96-97 -1.4726759841918344 2316 98-99 -1.3182500625757037 2316 100 -1.145739890804606 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 15276.0 3 3697.0 4 2610.0 5 2196.0 6 3739.0 7 18063.0 8 34491.0 9 39847.0 10 40432.0 11 38245.0 12 37114.0 13 39366.0 14 45301.0 15 54750.0 16 69469.0 17 86181.0 18 95921.0 19 107894.0 20 124610.0 21 153326.0 22 200758.0 23 295940.0 24 431503.0 25 512885.0 26 498082.0 27 433404.0 28 397701.0 29 396988.0 30 445206.0 31 539832.0 32 674751.0 33 874611.0 34 1205946.0 35 1827171.0 36 3187824.0 37 6066231.0 38 7913865.0 39 2425934.0 40 5232.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 14.98705816062921 32.57933785640523 27.356519696424712 25.077084286540842 2 20.374429384000678 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20-21 23.379340990366448 29.756289396030823 26.423646759057327 20.440722854545402 22-23 23.367329351446486 26.83396676571754 29.299185459267218 20.49951842356875 24-25 20.516541633606227 29.578455501165312 26.289776596767556 23.615226268460905 26-27 20.585174386233753 26.679859981470244 32.229479776967615 20.5054858553284 28-29 20.5610151507818 26.78445400710709 32.22020624748422 20.434324594626887 30-31 20.48626313524784 26.692264113102905 32.24179882352101 20.57967392812825 32-33 20.631521529142262 26.862821416965684 31.99761146139679 20.508045592495264 34-35 20.656007431663575 26.963240632940565 31.864466833211424 20.516285102184437 36-37 20.57274594794645 26.889487898202148 31.970622771658352 20.567143382193052 38-39 20.602407672102743 26.885601320336423 31.988878426081648 20.523112581479186 40-41 20.637933753592165 26.909699769324895 31.960700519765574 20.49166595731737 42-43 20.632079305905734 26.95953673538561 31.895739922680004 20.512644036028654 44-45 20.66513883511025 27.01168603769061 31.878633532161484 20.444541595037663 46-47 20.654648991626363 27.10747019338045 31.815947336525923 20.421933478467263 48-49 20.736330504600787 27.130442152494737 31.67176311463158 20.461464228272895 50-51 20.747136709750148 27.192496832825963 31.629156669167273 20.431209788256616 52-53 20.87903314619032 27.29478876436509 31.35038558389638 20.47579250554821 54-55 21.203361516416283 27.530669902482746 30.662583759386163 20.60338482171481 56-57 21.649693640224235 28.02349121575568 29.62545373035953 20.701361413660557 58-59 21.799270683210572 28.498740646696636 28.980745836863136 20.721242833229653 60-61 21.98606548589208 28.708327009960243 28.628457222155077 20.677150281992592 62-63 22.05490858802492 28.85669534640489 28.459745573357115 20.62865049221308 64-65 22.185784210560612 28.90124725326142 28.387732383440216 20.52523615273775 66-67 22.243174139363582 28.766420992558604 28.522474965597883 20.46792990247993 68-69 22.377188757130302 28.631947944692044 28.64564877509271 20.345214523084945 70-71 22.496458071979237 28.508177175967326 28.71561078247369 20.279753969579748 72-73 22.663613617579163 28.41693667837118 28.704367695003945 20.215082009045716 74-75 22.877027324071666 28.512876904002255 28.55188787532836 20.05820789659771 76-77 23.15792702008156 28.614155474756124 28.255277801970756 19.972639703191557 78-79 23.428178872348745 28.746819816389195 27.92375490870418 19.901246402557877 80-81 23.742224227262394 28.87390377200078 27.579809150686867 19.804062850049952 82-83 23.916437820773282 29.02397932900807 27.271887001361083 19.787695848857563 84-85 24.090704437780015 29.05609146452702 27.10939647532495 19.743807622368013 86-87 24.280187591270764 29.028258110302847 27.02313841765501 19.668415880771384 88-89 24.51188933500673 29.008623770255365 26.866672352640286 19.61281454209762 90-91 24.738310529691375 28.964978208049676 26.74757344585201 19.54913781640694 92-93 24.916653784584977 28.96913487351843 26.660188088630086 19.454023253266502 94-95 25.121806345312837 28.925209307099394 26.56929654361785 19.38368780396992 96-97 25.268298523294426 28.865198259109086 26.471645557907113 19.394857659689375 98-99 25.43312460794794 28.86892297022181 26.39391326908969 19.304039152740557 100 25.518877414363278 28.857233480089644 26.28329664055999 19.34059246498709 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 30672.0 1 22769.5 2 12731.0 3 9864.5 4 8988.0 5 9054.5 6 9327.5 7 9226.0 8 9163.0 9 9395.5 10 9889.5 11 12697.5 12 104327.0 13 129983.0 14 65091.5 15 68077.0 16 77479.0 17 86994.0 18 95817.0 19 104371.5 20 115010.5 21 131949.5 22 156478.0 23 185677.5 24 215276.0 25 248295.5 26 286602.0 27 331953.0 28 390494.5 29 449263.5 30 497063.5 31 543161.0 32 590649.5 33 631452.5 34 668636.0 35 713579.5 36 761151.0 37 806862.5 38 859422.0 39 894865.0 40 909049.0 41 939327.0 42 981070.5 43 991449.0 44 980277.5 45 984900.0 46 996645.5 47 999119.0 48 992570.0 49 980668.0 50 964016.5 51 944514.5 52 921023.5 53 876619.0 54 814338.5 55 752870.5 56 689138.5 57 619137.0 58 544075.5 59 462895.5 60 379603.5 61 306213.0 62 241095.0 63 181200.0 64 133427.5 65 97788.5 66 70661.5 67 52434.0 68 39669.0 69 30276.5 70 23574.5 71 18783.0 72 15595.5 73 12755.5 74 10436.0 75 8877.0 76 7085.5 77 5019.5 78 3662.0 79 2871.0 80 2246.5 81 1788.5 82 1490.5 83 1241.0 84 1047.5 85 884.0 86 718.0 87 606.0 88 533.0 89 480.5 90 419.5 91 349.0 92 283.5 93 226.0 94 209.5 95 195.0 96 165.5 97 127.5 98 90.5 99 75.0 100 44.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4031875536863271 2 0.3830624221198981 3 0.16878054378882418 4 0.17496188287814052 5 0.2750968500659297 6 0.10224084786981649 7 0.1361836916783501 8 0.14181300379276607 9 0.135144381633013 10-11 0.13163117292238175 12-13 0.13367060591298582 14-15 0.13979231245871726 16-17 0.14417104494480956 18-19 0.15546544869979245 20-21 0.13705091924077073 22-23 0.09927966613408558 24-25 0.09409333862915754 26-27 0.08855262343663917 28-29 0.09846184839349245 30-31 0.10286273011005918 32-33 0.10413034760797851 34-35 0.10477778665261475 36-37 0.1004109806752394 38-39 0.09990495594824739 40-41 0.10092382055006967 42-43 0.06403001772756256 44-45 0.06693327070666813 46-47 0.08508371318695668 48-49 0.08905524058971201 50-51 0.0983170265019291 52-53 0.10358513578091644 54-55 0.10621407905953141 56-57 0.10682403479105711 58-59 0.1061374086463508 60-61 0.10410479080358499 62-63 0.10853804447238352 64-65 0.11552016343269729 66-67 0.12412088000460159 68-69 0.12931572644432746 70-71 0.12777039167199838 72-73 0.11456093137445994 74-75 0.11191665401320885 76-77 0.11408216723882104 78-79 0.11255046276216851 80-81 0.10692796579559082 82-83 0.10995900279666407 84-85 0.11600915029009358 86-87 0.12946054833589082 88-89 0.1375245720155309 90-91 0.144268160801505 92-93 0.14353382862193076 94-95 0.13363141881291574 96-97 0.13010117223268877 98-99 0.1249506242539117 100 0.11922760385671943 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2.9346392E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.18178017101286 #Duplication Level Percentage of deduplicated Percentage of total 1 67.63016389423568 27.17500378527749 2 16.901632702267655 13.582753795474419 3 6.0668754110390015 7.313335622738773 4 2.714845637948711 4.36349322489153 5 1.5674524569805375 3.1491515027452976 6 0.9771009802394219 2.355699407571698 7 0.7072049391047477 1.9891727379273059 8 0.5043480207957948 1.621248104104164 9 0.41365735840065454 1.4959340137239283 >10 2.1927107053948744 15.849086176524024 >50 0.18312615265661228 5.163374803183783 >100 0.13814043901356524 9.703549601083454 >500 0.0021814311798934744 0.5074580652931936 >1k 5.175698709710127E-4 0.437882550076422 >5k 1.6920348808551176E-5 0.044056854907438533 >10k+ 2.538052320778843E-5 5.248799754476993 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 1476901 5.032649328748829 No Hit AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 43432 0.14799775045600155 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 5.622496966577697E-4 1.703786959568999E-5 0.0 6.815147838275997E-6 3.066816527224198E-5 2 5.792875662534598E-4 2.044544351482799E-5 0.0 6.815147838275997E-6 3.066816527224198E-5 3 5.929178619300117E-4 2.044544351482799E-5 0.0 1.0222721757413995E-5 4.089088702965598E-5 4 6.031405836874257E-4 3.066816527224198E-5 0.0 1.3630295676551993E-5 4.7706034867931974E-5 5 6.133633054448397E-4 3.066816527224198E-5 0.0 1.3630295676551993E-5 4.7706034867931974E-5 6 6.167708793639777E-4 3.407573919137998E-5 0.0 1.3630295676551993E-5 4.7706034867931974E-5 7 6.167708793639777E-4 4.089088702965598E-5 0.0 1.3630295676551993E-5 4.7706034867931974E-5 8 6.235860272022537E-4 4.4298460948793974E-5 0.0 1.3630295676551993E-5 5.792875662534597E-5 9 6.304011750405296E-4 5.1113608787069974E-5 0.0 1.3630295676551993E-5 5.792875662534597E-5 10-11 6.678844881510477E-4 6.304011750405298E-5 0.0 1.874165655525899E-5 6.133633054448397E-5 12-13 7.053678012615657E-4 6.815147838275996E-5 0.0 2.3853017433965987E-5 6.474390446362197E-5 14-15 7.309246056551007E-4 7.326283926146695E-5 0.0 2.7260591353103987E-5 6.644769142319097E-5 16-17 7.547776230890665E-4 8.859692189758795E-5 0.0 3.407573919137998E-5 6.985526534232896E-5 18-19 8.024836579569987E-4 9.541206973586395E-5 0.0 4.259467398922498E-5 7.496662622103595E-5 20-21 8.570048406632066E-4 1.0393100453370894E-4 0.0 4.7706034867931974E-5 7.837420014017396E-5 22-23 9.421941886416565E-4 1.1756130021026094E-4 0.0 5.6224969665776976E-5 8.178177405931196E-5 24-25 0.0011892432977791616 1.2778402196767495E-4 0.0 6.304011750405298E-5 8.178177405931196E-5 26-27 0.002107584468986852 1.6697112203776192E-4 0.0 6.815147838275996E-5 8.518934797844996E-5 28-29 0.004825124669499406 1.993430742695729E-4 0.0 7.155905230189796E-5 9.541206973586395E-5 30-31 0.012057700312869808 2.2319609170353888E-4 0.0 7.496662622103596E-5 9.711585669543296E-5 32-33 0.028049444715384432 2.3512260042052188E-4 0.0 8.007798709974297E-5 1.1756130021026094E-4 34-35 0.05776008171634864 2.4704910913750486E-4 0.0 8.348556101888096E-5 1.2948780892724394E-4 36-37 0.10698759833917573 2.5216047001621185E-4 0.0 9.541206973586396E-5 1.3289538284638194E-4 38-39 0.17864887785864786 2.589756178544879E-4 0.0 1.0222721757413995E-4 1.3800674372508893E-4 40-41 0.2763695789247278 2.657907656927639E-4 0.0 1.0393100453370894E-4 1.465256785229339E-4 42-43 0.40208009216260726 2.7601348745017785E-4 0.0 1.0733857845284695E-4 1.5334082636120992E-4 44-45 0.5628340955849018 2.896437831267298E-4 0.0 1.1244993933155394E-4 1.5334082636120992E-4 46-47 0.7622453213328575 3.0327407880328185E-4 0.0 1.1756130021026094E-4 1.6697112203776192E-4 48-49 1.0083658665773974 3.169043744798338E-4 0.0 1.2096887412939894E-4 1.8230520467388291E-4 50-51 1.2946804499851292 3.2883088319681685E-4 0.0 1.2608023500810592E-4 1.908241394717279E-4 52-53 1.6155086458328505 3.3734981799466183E-4 0.0 1.2608023500810592E-4 1.908241394717279E-4 54-55 1.9730432279375263 3.492763267116448E-4 0.0 1.2778402196767495E-4 1.925279264312969E-4 56-57 2.3811410956413313 3.5949904846905884E-4 0.0 1.3630295676551993E-4 2.061582221078489E-4 58-59 2.845569567802406 3.816482789434558E-4 0.0 1.4141431764422692E-4 2.2319609170353888E-4 60-61 3.3553204768749767 3.850558528625938E-4 0.0 1.4311810460379592E-4 2.300112395418149E-4 62-63 3.9009309900855955 3.969823615795768E-4 0.0 1.4311810460379592E-4 2.3682638738009088E-4 64-65 4.47889812144539 4.089088702965598E-4 0.0 1.4311810460379592E-4 2.4193774825879787E-4 66-67 5.097904369300322 4.259467398922498E-4 0.0 1.4652567852293394E-4 2.4534532217793586E-4 68-69 5.759367284400754 4.327618877305258E-4 0.0 1.4652567852293394E-4 2.4534532217793586E-4 70-71 6.4564972757128025 4.412808225283708E-4 3.4075739191379983E-6 1.5674840028034791E-4 2.4534532217793586E-4 72-73 7.187842716746918 4.532073312453538E-4 3.4075739191379983E-6 1.601559741994859E-4 2.4875289609707386E-4 74-75 7.943738024081462 4.634300530027678E-4 3.4075739191379983E-6 1.6185976115905493E-4 2.5216047001621185E-4 76-77 8.726907553064786 4.634300530027678E-4 3.4075739191379983E-6 1.6526733507819292E-4 2.5216047001621185E-4 78-79 9.54269267581514 4.634300530027678E-4 3.4075739191379983E-6 1.6697112203776192E-4 2.5216047001621185E-4 80-81 10.383336050305605 4.6854141388147477E-4 3.4075739191379983E-6 1.754900568356069E-4 2.5727183089491884E-4 82-83 11.24489340972478 4.8046792259845775E-4 3.4075739191379983E-6 1.8060141771431392E-4 2.6919833961190187E-4 84-85 12.126911546741418 4.906906443558717E-4 3.4075739191379983E-6 1.840089916334519E-4 2.8282863528845384E-4 86-87 13.028763467754402 5.077285139515617E-4 3.4075739191379983E-6 1.908241394717279E-4 2.8453242224802283E-4 88 13.715079523234065 5.145436617898378E-4 3.4075739191379983E-6 1.942317133908659E-4 2.896437831267299E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 190335 0.0 84.56004 8 AGTGGTA 192480 0.0 83.783226 5 TGGTATC 192670 0.0 83.35114 7 GTATCAA 193860 0.0 82.90527 9 GTGGTAT 196040 0.0 82.295685 6 AAGCAGT 197915 0.0 81.926895 1 GCAGTGG 200260 0.0 80.94929 3 CAGTGGT 203655 0.0 79.10698 4 AGCAGTG 208860 0.0 77.67 2 CAACGCA 187365 0.0 44.60318 12-13 ATCAACG 188815 0.0 44.345512 10-11 ACGCAGA 189910 0.0 44.035236 14-15 AGAGTAC 190545 0.0 43.232018 18-19 TCAACGC 187155 0.0 43.10095 12-13 AACGCAG 188965 0.0 42.79873 14-15 GAGTACT 185770 0.0 42.527126 20-21 AGTACTT 193685 0.0 42.13982 20-21 CGCAGAG 191935 0.0 42.04982 16-17 TATCAAC 193930 0.0 41.421116 10-11 GTACTTT 195775 0.0 40.29878 22-23 >>END_MODULE