FastQCFastQC Report
Thu 26 May 2016
SRR1660730_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660730_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29346392
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT13078124.4564660623357035No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT472570.16103172069670438No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1777000.082.504518
AGTGGTA1777050.081.8136445
AAGCAGT1728550.081.702791
TGGTATC1798450.081.4466257
GTATCAA1805900.081.1085369
GTGGTAT1811250.080.3362356
GCAGTGG1810800.080.263343
CAGTGGT1881400.077.149474
AGCAGTG1884150.076.815862
CAACGCA1729750.044.15097412-13
ATCAACG1747550.043.86779810-11
ACGCAGA1761600.043.42336314-15
AGAGTAC1790550.042.6534218-19
TCAACGC1727600.042.3012912-13
AACGCAG1744050.041.95885514-15
GAGTACT1736100.041.77350620-21
AGTACTT1819900.041.63226720-21
CGCAGAG1772000.041.29183616-17
TATCAAC1801350.040.6462810-11
GTACTTT1834800.039.4945922-23
Produced by FastQC (version 0.11.5)