##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660730_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 29346392 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.201206301612817 34.0 31.0 34.0 27.0 34.0 2 31.860714530086014 34.0 31.0 34.0 28.0 34.0 3 31.624884653622836 34.0 31.0 34.0 30.0 34.0 4 35.56025279018968 37.0 35.0 37.0 33.0 37.0 5 35.904904800562875 37.0 35.0 37.0 33.0 37.0 6 35.36155664382865 37.0 35.0 37.0 33.0 37.0 7 35.58331487564127 37.0 35.0 37.0 35.0 37.0 8 35.94476060293886 37.0 35.0 37.0 35.0 37.0 9 37.75446961248252 39.0 38.0 39.0 35.0 39.0 10-11 37.78946309651967 39.0 37.5 39.0 35.0 39.0 12-13 37.845056097526395 39.0 38.0 39.0 35.0 39.0 14-15 39.28753299553826 41.0 39.0 41.0 36.0 41.0 16-17 39.25521023163597 41.0 39.0 41.0 36.0 41.0 18-19 39.256800682686986 41.0 39.0 41.0 36.0 41.0 20-21 39.20160848052463 40.5 39.0 41.0 36.0 41.0 22-23 39.0938215334955 40.0 39.0 41.0 35.5 41.0 24-25 39.097442830450845 40.0 39.0 41.0 36.0 41.0 26-27 39.03751348717757 40.0 39.0 41.0 35.5 41.0 28-29 38.95329373028207 40.0 38.5 41.0 35.0 41.0 30-31 38.856140083591875 40.0 38.0 41.0 35.0 41.0 32-33 38.733373100856824 40.0 38.0 41.0 35.0 41.0 34-35 38.64433779457454 40.0 38.0 41.0 35.0 41.0 36-37 38.523357045050034 40.0 38.0 41.0 34.0 41.0 38-39 38.17149017841784 40.0 37.0 41.0 34.0 41.0 40-41 38.04021107603279 40.0 37.0 41.0 33.5 41.0 42-43 37.87996681159306 40.0 37.0 41.0 33.0 41.0 44-45 37.7559086139107 40.0 36.5 41.0 33.0 41.0 46-47 37.60077421101715 40.0 36.0 41.0 33.0 41.0 48-49 37.653744231999625 40.0 36.0 41.0 33.0 41.0 50-51 37.66211335281012 40.0 36.0 41.0 33.0 41.0 52-53 37.4341798814655 40.0 36.0 41.0 32.5 41.0 54-55 36.97658523064777 40.0 36.0 41.0 31.0 41.0 56-57 36.556300805223344 39.5 35.5 41.0 30.0 41.0 58-59 36.43413369180102 39.0 35.0 41.0 30.0 41.0 60-61 36.289607952486975 39.0 35.0 41.0 29.0 41.0 62-63 36.05430396690673 39.0 35.0 41.0 29.0 41.0 64-65 35.79030108709786 38.5 35.0 40.5 29.0 41.0 66-67 35.50764008740836 37.5 35.0 40.0 28.5 41.0 68-69 35.23211681354219 37.0 35.0 40.0 28.0 41.0 70-71 34.9203616580873 36.5 34.5 39.0 28.0 41.0 72-73 34.572011697383445 36.0 34.0 39.0 28.0 41.0 74-75 34.19089842458317 35.5 34.0 39.0 27.0 40.0 76-77 32.47293910951643 34.5 32.0 36.5 25.0 39.0 78-79 33.41250253182742 35.0 33.5 37.0 26.0 39.0 80-81 33.313922031710064 35.0 34.0 36.5 26.0 39.0 82-83 33.05498370975212 35.0 34.0 36.0 26.0 38.0 84-85 32.799979840792695 35.0 34.0 36.0 26.0 37.0 86-87 32.57719603145763 35.0 33.5 35.5 26.0 37.0 88-89 32.397960216029276 35.0 33.0 35.0 25.0 36.5 90-91 32.22917699729493 35.0 33.0 35.0 25.0 36.0 92-93 32.07993446008627 35.0 33.0 35.0 25.0 36.0 94-95 31.96741146236989 35.0 33.0 35.0 24.5 36.0 96-97 31.8625760536423 35.0 33.0 35.0 24.0 35.0 98-99 31.76523456443981 35.0 33.0 35.0 24.0 35.0 100 31.706734919917924 35.0 33.0 35.0 23.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.5543897663764987 1101 2 1.1696644012972506 1101 3 1.542978364267551 1101 4 0.9552341429355593 1101 5 0.5694971455755677 1101 6 1.10840327787227 1101 7 0.8237360892246528 1101 8 0.48089522669744156 1101 9 0.49876566240456555 1101 10-11 0.40662981661665754 1101 12-13 0.39873965939533207 1101 14-15 0.5097107117702109 1101 16-17 0.5277702058456981 1101 18-19 0.4998288048374704 1101 20-21 0.5205136908140844 1101 22-23 0.4822190763132781 1101 24-25 0.4811187739018692 1101 26-27 0.4862448264625172 1101 28-29 0.47577364104628117 1101 30-31 0.48738270808426165 1101 32-33 0.5093122063294118 1101 34-35 0.5465786131229322 1101 36-37 0.5117878930819941 1101 38-39 0.5253824124741158 1101 40-41 0.5416674532638339 1101 42-43 0.5026458447512212 1101 44-45 0.4600884650504824 1101 46-47 0.4937967459343966 1101 48-49 0.46605654134638996 1101 50-51 0.44468540741331 1101 52-53 0.44315204843699263 1101 54-55 0.43449685686197626 1101 56-57 0.4374161898091202 1101 58-59 0.3989776568967258 1101 60-61 0.43932850313109384 1101 62-63 0.3965662896378106 1101 64-65 0.37200179148089774 1101 66-67 0.3857413752961705 1101 68-69 0.3394302664844062 1101 70-71 0.35683580939017645 1101 72-73 0.30205275341856463 1101 74-75 0.33639287846868626 1101 76-77 0.2741033928172385 1101 78-79 0.35767387409485707 1101 80-81 0.36959665663631824 1101 82-83 0.3559437572667292 1101 84-85 0.3363946771014099 1101 86-87 0.3406909300783596 1101 88-89 0.31976615652410345 1101 90-91 0.32035366094268625 1101 92-93 0.30419909868971473 1101 94-95 0.2935003323206793 1101 96-97 0.2834536200173652 1101 98-99 0.27784518514715373 1101 100 0.27865708538373823 1102 1 1.5140155178813082 1102 2 1.1463030582761071 1102 3 1.520491549456441 1102 4 0.9095054421558473 1102 5 0.5093711262517289 1102 6 1.0537657594512595 1102 7 0.7731372047417011 1102 8 0.44949923609132725 1102 9 0.4462808588183904 1102 10-11 0.3625984830023583 1102 12-13 0.3509145042919144 1102 14-15 0.45520244366716867 1102 16-17 0.46957256602618 1102 18-19 0.4207929237263599 1102 20-21 0.42495964400943365 1102 22-23 0.4256463350938944 1102 24-25 0.4338963834930851 1102 26-27 0.44687449240186794 1102 28-29 0.45030610479861366 1102 30-31 0.44228068355583616 1102 32-33 0.4337347181727722 1102 34-35 0.3940501381284349 1102 36-37 0.3922725925989283 1102 38-39 0.4348391902576907 1102 40-41 0.42984655754175094 1102 42-43 0.44908148594667097 1102 44-45 0.43437839318816174 1102 46-47 0.4665074480640996 1102 48-49 0.4149866271781164 1102 50-51 0.35139256723656587 1102 52-53 0.38118328192175 1102 54-55 0.41265041448365025 1102 56-57 0.44883596233303535 1102 58-59 0.43561248489267257 1102 60-61 0.4778949196246245 1102 62-63 0.43684628454870733 1102 64-65 0.3833567701618321 1102 66-67 0.3622061422545997 1102 68-69 0.3705713955520693 1102 70-71 0.3188597948927878 1102 72-73 0.27866602305562793 1102 74-75 0.31788744096951405 1102 76-77 0.1570397248112485 1102 78-79 0.39807315977263613 1102 80-81 0.39471481873935943 1102 82-83 0.37876167398779614 1102 84-85 0.3451864152551778 1102 86-87 0.35696834772796393 1102 88-89 0.3539899209448123 1102 90-91 0.3736161745182258 1102 92-93 0.36286470949793426 1102 94-95 0.31008251798936826 1102 96-97 0.3582352026971911 1102 98-99 0.35262380826219086 1102 100 0.3562736912929374 1103 1 1.4933702549165666 1103 2 1.0967678953665505 1103 3 1.4385108333385652 1103 4 0.8905071327471319 1103 5 0.48364829652822294 1103 6 1.0302494131648672 1103 7 0.7544028036203301 1103 8 0.4138762563871694 1103 9 0.4098811911945148 1103 10-11 0.35043108480463303 1103 12-13 0.3128542093844757 1103 14-15 0.3601050826434147 1103 16-17 0.39750893092767114 1103 18-19 0.35499986732420297 1103 20-21 0.368467493309673 1103 22-23 0.3636214642704587 1103 24-25 0.35304675550326436 1103 26-27 0.3713591521383037 1103 28-29 0.35200977982167814 1103 30-31 0.347597870509766 1103 32-33 0.37734319811845296 1103 34-35 0.3969063248386817 1103 36-37 0.36339690246067136 1103 38-39 0.3797114623917395 1103 40-41 0.3945587371154815 1103 42-43 0.4014412881327374 1103 44-45 0.37149752370683586 1103 46-47 0.4090281427413771 1103 48-49 0.3529265243800168 1103 50-51 0.3483411386158153 1103 52-53 0.3666948872737592 1103 54-55 0.36382066103108457 1103 56-57 0.3607651442389397 1103 58-59 0.34566918212478726 1103 60-61 0.33437316627592395 1103 62-63 0.2966010951904323 1103 64-65 0.2787291900650928 1103 66-67 0.300710629418667 1103 68-69 0.3124346397975941 1103 70-71 0.3360039569457456 1103 72-73 0.3102956469142768 1103 74-75 0.31550033454139026 1103 76-77 0.16152085465294164 1103 78-79 0.3480726364644724 1103 80-81 0.3827383522876886 1103 82-83 0.3680651400095414 1103 84-85 0.3468958710217862 1103 86-87 0.3023999195706679 1103 88-89 0.3249755920099133 1103 90-91 0.3247675550935156 1103 92-93 0.33767239233656454 1103 94-95 0.3135793614536162 1103 96-97 0.33820165193169416 1103 98-99 0.32471699111573216 1103 100 0.34219622646046943 1104 1 1.4417676288729275 1104 2 1.0698127982674173 1104 3 1.4271180947333058 1104 4 0.8886097242939428 1104 5 0.48133439041422577 1104 6 1.0033716980304632 1104 7 0.7383685489026703 1104 8 0.41474169469621813 1104 9 0.3916889547637439 1104 10-11 0.3179341724153204 1104 12-13 0.2964483339148174 1104 14-15 0.35275006142405374 1104 16-17 0.3654027068681529 1104 18-19 0.3441043000663413 1104 20-21 0.3426672012795464 1104 22-23 0.34397262906332315 1104 24-25 0.33689827594505317 1104 26-27 0.3537253319111855 1104 28-29 0.2824355729005106 1104 30-31 0.3320243006344157 1104 32-33 0.3640937101014998 1104 34-35 0.3636613100764592 1104 36-37 0.36896663950251707 1104 38-39 0.3705014387444123 1104 40-41 0.33765163869095005 1104 42-43 0.36399242654858455 1104 44-45 0.3327714419628265 1104 46-47 0.37622794839588636 1104 48-49 0.30384899490849193 1104 50-51 0.31159645583978346 1104 52-53 0.36231621515143075 1104 54-55 0.3312495691318702 1104 56-57 0.38008983411043573 1104 58-59 0.39620879332124304 1104 60-61 0.4113494981193142 1104 62-63 0.3409045937307553 1104 64-65 0.27765112535401215 1104 66-67 0.30783151297316635 1104 68-69 0.2762992296304674 1104 70-71 0.29078000301049656 1104 72-73 0.27745626085415864 1104 74-75 0.30808443948188113 1104 76-77 0.22539479172056076 1104 78-79 0.3150830425805822 1104 80-81 0.3301261307423431 1104 82-83 0.3385823750292971 1104 84-85 0.30405219323981214 1104 86-87 0.32112311213135314 1104 88-89 0.3169475099123318 1104 90-91 0.30636595608662986 1104 92-93 0.3086173510439707 1104 94-95 0.27423845650445955 1104 96-97 0.26676514151560227 1104 98-99 0.3095059144382155 1104 100 0.32532644346911255 1105 1 1.3524026448373725 1105 2 0.9964533436218552 1105 3 1.3568959335745951 1105 4 0.841148238946495 1105 5 0.42525799549073895 1105 6 0.9885706551466811 1105 7 0.6922338356596995 1105 8 0.34893982939525614 1105 9 0.3426422750170204 1105 10-11 0.25776827032687066 1105 12-13 0.22663022560323753 1105 14-15 0.2960521790340209 1105 16-17 0.284708365650161 1105 18-19 0.25417987569671396 1105 20-21 0.2934918924472498 1105 22-23 0.28501271303017717 1105 24-25 0.2797164272371262 1105 26-27 0.2806662803582398 1105 28-29 0.26531015327751106 1105 30-31 0.289532015173279 1105 32-33 0.3086690271089765 1105 34-35 0.29751805729598146 1105 36-37 0.29465492185156705 1105 38-39 0.34677478694586483 1105 40-41 0.355127278663808 1105 42-43 0.30542333707644787 1105 44-45 0.29365367142138865 1105 46-47 0.30705576757038955 1105 48-49 0.31383495932622907 1105 50-51 0.3053727779003097 1105 52-53 0.25957865344259545 1105 54-55 0.36157900198234216 1105 56-57 0.37930881895450597 1105 58-59 0.34645409949414585 1105 60-61 0.334383282598786 1105 62-63 0.30438298333735503 1105 64-65 0.27984304521127257 1105 66-67 0.29252874924169703 1105 68-69 0.300878539516269 1105 70-71 0.2959700213671468 1105 72-73 0.2504606479231697 1105 74-75 0.2824700000605205 1105 76-77 0.20833471429187256 1105 78-79 0.3060454015633667 1105 80-81 0.3086685006172445 1105 82-83 0.33106910459525096 1105 84-85 0.31932908646084 1105 86-87 0.31596429163064244 1105 88-89 0.27723182047540007 1105 90-91 0.2935120020732569 1105 92-93 0.28579970552087275 1105 94-95 0.27616788292265326 1105 96-97 0.29164880725282316 1105 98-99 0.25949617559028226 1105 100 0.26474159498556205 1106 1 1.3642669226575386 1106 2 0.9944543308624993 1106 3 1.337000174499682 1106 4 0.8157184272501965 1106 5 0.4285749960204015 1106 6 0.9527852741640714 1106 7 0.6692435216082586 1106 8 0.32606552440088166 1106 9 0.3038094429793645 1106 10-11 0.201265315711737 1106 12-13 0.17373419528310308 1106 14-15 0.2464396712353789 1106 16-17 0.23327448314969956 1106 18-19 0.18865344565188735 1106 20-21 0.22233379870417025 1106 22-23 0.23806531373988093 1106 24-25 0.2184781862538543 1106 26-27 0.20463948383696362 1106 28-29 0.2213505888133369 1106 30-31 0.23329066771165685 1106 32-33 0.2888605439585774 1106 34-35 0.2799936595015353 1106 36-37 0.2568056886860077 1106 38-39 0.2749345731473767 1106 40-41 0.2640771677345768 1106 42-43 0.2997754086762896 1106 44-45 0.30292160805853285 1106 46-47 0.27880588249674076 1106 48-49 0.2675733886090086 1106 50-51 0.2613848906444858 1106 52-53 0.22193462718662005 1106 54-55 0.27812606663716366 1106 56-57 0.32282140352891986 1106 58-59 0.296377453500007 1106 60-61 0.30154721198268675 1106 62-63 0.27381465427341567 1106 64-65 0.22933872203508798 1106 66-67 0.20558558338405675 1106 68-69 0.2448902636231196 1106 70-71 0.2523284612105172 1106 72-73 0.18448610646120045 1106 74-75 0.17613280648227914 1106 76-77 0.04544786448413163 1106 78-79 0.23541483997649948 1106 80-81 0.2614255211606604 1106 82-83 0.27107030261763754 1106 84-85 0.2760304483155309 1106 86-87 0.28017838907013726 1106 88-89 0.2654278436096291 1106 90-91 0.2613915085092984 1106 92-93 0.24031334538825178 1106 94-95 0.22710294724198477 1106 96-97 0.26447712316754846 1106 98-99 0.2691910231102064 1106 100 0.27644764065912497 1107 1 1.3008712010557026 1107 2 0.9168907771864347 1107 3 1.2820954735375132 1107 4 0.7753934259846815 1107 5 0.3827146786072717 1107 6 0.9311240988808223 1107 7 0.644892821955132 1107 8 0.29940689347217386 1107 9 0.24928525354819442 1107 10-11 0.17419544113942464 1107 12-13 0.12896091831234457 1107 14-15 0.1793016435697652 1107 16-17 0.16950382884975568 1107 18-19 0.13778435084419272 1107 20-21 0.14145377677931492 1107 22-23 0.18723106237192866 1107 24-25 0.1700863104877257 1107 26-27 0.1419850467669974 1107 28-29 0.13705414185414355 1107 30-31 0.1493003285862926 1107 32-33 0.15411254802140206 1107 34-35 0.18195880038374668 1107 36-37 0.1670432912251414 1107 38-39 0.1724092943323825 1107 40-41 0.16187577321678503 1107 42-43 0.18334501598229025 1107 44-45 0.14714746239831555 1107 46-47 0.21037855511951165 1107 48-49 0.1868511940390576 1107 50-51 0.1636155137170192 1107 52-53 0.18009106945644504 1107 54-55 0.21106597854873144 1107 56-57 0.18580203223112335 1107 58-59 0.1400401831283844 1107 60-61 0.13997668436800126 1107 62-63 0.1293054294259477 1107 64-65 0.11207685213676655 1107 66-67 0.11738986039929244 1107 68-69 0.12723326491208553 1107 70-71 0.13777184919256058 1107 72-73 0.10651734324786588 1107 74-75 0.11353860647914615 1107 76-77 -0.024098957167417723 1107 78-79 0.09309675374599635 1107 80-81 0.14530213067013165 1107 82-83 0.1660472689246717 1107 84-85 0.16817534575689308 1107 86-87 0.15289892740616295 1107 88-89 0.1677912447538361 1107 90-91 0.17136361730295846 1107 92-93 0.15925926341491703 1107 94-95 0.11982363154282538 1107 96-97 0.15364188850140792 1107 98-99 0.17218531075508636 1107 100 0.1705478034778487 1108 1 1.2627062099366562 1108 2 0.8920787789388775 1108 3 1.2417459976192369 1108 4 0.7445844304739211 1108 5 0.35121445478838353 1108 6 0.8819091698183783 1108 7 0.6167689568254673 1108 8 0.24317248457804652 1108 9 0.20493131906594897 1108 10-11 0.10937452514787083 1108 12-13 0.10788231780633595 1108 14-15 0.11585151037476749 1108 16-17 0.11627840311554394 1108 18-19 0.07772810550661546 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-1.0861337367867279 2316 80-81 -1.3666030552349255 2316 82-83 -1.4208911781485583 2316 84-85 -1.0515164763271585 2316 86-87 -0.8344509197456702 2316 88-89 -0.7036047937152503 2316 90-91 -0.7847626888859125 2316 92-93 -0.8901368435246972 2316 94-95 -0.8973017608859948 2316 96-97 -0.9862241040772908 2316 98-99 -1.134073491585621 2316 100 -1.0747270300460805 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 64.0 7 914.0 8 1072.0 9 439.0 10 386.0 11 652.0 12 1026.0 13 1503.0 14 3405.0 15 8634.0 16 18295.0 17 26570.0 18 32535.0 19 41956.0 20 57640.0 21 82532.0 22 124094.0 23 205241.0 24 341280.0 25 447600.0 26 451676.0 27 389706.0 28 353884.0 29 376051.0 30 438611.0 31 536801.0 32 664143.0 33 851493.0 34 1163905.0 35 1786877.0 36 3213407.0 37 6510364.0 38 8681681.0 39 2526341.0 40 5614.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 15.039850168576422 31.651717290760846 27.660338026687853 25.648094513974883 2 20.566302167360163 32.12708078700553 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23.150325578450563 29.674751403553973 26.65818950674423 20.516733511251235 22-23 23.110142087544393 27.006080937513943 29.30406798562664 20.57970898931502 24-25 20.551897497351153 29.473408123931627 26.48836879360364 23.486325585113583 26-27 20.614035102665298 26.828840710916026 31.970176798024987 20.586947388393693 28-29 20.603093916679846 26.94047088772704 31.934337394827818 20.522097800765298 30-31 20.51990071003144 26.827562416005012 31.96562506890072 20.686911805062827 32-33 20.716710295710907 27.00403162598611 31.701657802574264 20.57760027572872 34-35 20.7023235237351 27.121998657524692 31.571955158196314 20.60372266054389 36-37 20.61649009784722 27.053347746725052 31.672363391402648 20.657798764025078 38-39 20.6441861235823 27.040206278050594 31.72770561247401 20.587901985893097 40-41 20.67496446209362 27.06488330722523 31.685405806205345 20.5747464244758 42-43 20.665956201323112 27.111572568407876 31.622523652039074 20.599947578229933 44-45 20.717027168502895 27.164100530842315 31.595673708676014 20.523198591978772 46-47 20.750229584656722 27.264557231830615 31.46843883762825 20.516774345884418 48-49 20.769809828543572 27.291368545030053 31.361423773967445 20.577397852458926 50-51 20.783712196757325 27.391844047818324 31.283817097992923 20.540626657431428 52-53 20.893720852729615 27.493857622034863 31.020976112493436 20.59144541274209 54-55 21.15949496015144 27.678226875931323 30.406695437233132 20.755582726684107 56-57 21.544269669753323 28.172028798392525 29.404164836749707 20.87953669510445 58-59 21.628219610865564 28.657254488091393 28.766587858369046 20.947938042673993 60-61 21.6893475703774 28.897073511266736 28.42635320712651 20.98722571122936 62-63 21.711028977881544 29.044955658707316 28.24818342362071 20.995831939790424 64-65 21.739335795722916 29.14566876990805 28.116561743234097 20.998433691134938 66-67 21.678099186943946 29.04731596249816 28.232393378132254 21.042191472425642 68-69 21.651456124327222 28.941131127046127 28.34705913036541 21.060353618261242 70-71 21.616327321371102 28.86010381067507 28.40540744842668 21.118161419527155 72-73 21.604433668687342 28.803346740742096 28.383220767226767 21.20899882334379 74-75 21.66243944638212 28.863738326131237 28.262568138269863 21.211254089216784 76-77 21.743124934130712 28.92192855795333 28.070531682398524 21.264414825517434 78-79 21.84110997695344 29.059908947735757 27.760042429369136 21.338938645941663 80-81 21.965395683389467 29.189396402895014 27.463060392849208 21.382147520866308 82-83 21.97739380205733 29.27816298403545 27.18453847351136 21.559904740395865 84-85 22.010952522607603 29.271181499834913 27.053252800209222 21.66461317734826 86-87 22.05379891514461 29.213981897164825 26.991864726768217 21.74035446092235 88-89 22.106614413106875 29.198744557477312 26.87586219183475 21.818778837581064 90-91 22.231411731433955 29.091372118062992 26.771178169012995 21.906037981490066 92-93 22.304096010729083 29.02791653222181 26.756272097730893 21.91171535931821 94-95 22.412783193537507 28.92091569931502 26.717021467414565 21.94927963973291 96-97 22.47834871573954 28.77025131624317 26.71328489619184 22.038115071825455 98-99 22.49509963083931 28.690613110025048 26.761119174223364 22.05316808491228 100 22.4528700535707 28.636312209484604 26.724816319297716 22.186001417646978 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 18423.0 1 15159.0 2 9865.0 3 7235.5 4 6455.5 5 6500.5 6 6866.0 7 6910.0 8 6827.5 9 7129.5 10 8050.5 11 14928.5 12 104213.0 13 122524.5 14 56949.0 15 60965.0 16 70131.0 17 79216.5 18 88263.5 19 97302.0 20 109129.5 21 127404.5 22 153474.0 23 183591.5 24 214318.0 25 248569.0 26 289204.0 27 337929.0 28 396021.0 29 454340.0 30 503525.0 31 550712.5 32 599337.5 33 638496.0 34 673730.5 35 717848.0 36 764509.0 37 810226.0 38 864130.5 39 900864.0 40 917744.5 41 952726.0 42 997389.0 43 1006833.0 44 994846.5 45 999394.5 46 1007508.0 47 1004977.5 48 994865.5 49 982227.0 50 967494.0 51 952563.0 52 930662.0 53 884196.0 54 821731.0 55 757889.0 56 690149.5 57 617068.0 58 539454.5 59 457544.0 60 373153.5 61 298648.5 62 233440.0 63 173389.0 64 126116.0 65 90858.5 66 64292.5 67 46954.5 68 34496.5 69 25270.5 70 19108.5 71 14837.0 72 11969.5 73 9459.0 74 7410.0 75 6083.0 76 4557.5 77 2761.5 78 1508.0 79 940.5 80 580.0 81 382.0 82 275.0 83 177.5 84 127.0 85 88.5 86 53.0 87 36.0 88 25.0 89 19.0 90 13.5 91 13.0 92 6.5 93 4.0 94 5.5 95 4.0 96 2.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3286707272226173 2 0.006290381454728745 3 2.421681002557316 4 9.064146624907076E-4 5 3.066816527224198E-5 6 0.008716574085155 7 0.007759045813877222 8 2.8282863528845384E-4 9 0.0 10-11 8.007798709974297E-5 12-13 5.111360878706998E-6 14-15 0.0 16-17 4.770603486793198E-5 18-19 0.0 20-21 2.913475700862989E-4 22-23 4.259467398922498E-5 24-25 4.958020052345788E-4 26-27 0.0033564603103509285 28-29 0.0060484437064699465 30-31 0.005571383357790627 32-33 0.015363047014433666 34-35 0.009662175847715795 36-37 0.004975057921941478 38-39 0.005228922178917258 40-41 0.00808617291011447 42-43 0.018838772411954422 44-45 0.03374350073426403 46-47 0.033118210920102205 48-49 0.03979875958857225 50-51 0.039720385388432075 52-53 0.06192924840641398 54-55 0.03304324429388117 56-57 0.02211685852216518 58-59 0.016828303799663005 60-61 0.017681901066407074 62-63 0.024549866300429712 64-65 0.04462899561895037 66-67 0.0474078721500074 68-69 0.05030260619431513 70-71 0.06068548392592861 72-73 0.06838660098318049 74-75 0.05938038311489876 76-77 0.059540539089098246 78-79 0.05372040283521054 80-81 0.051583853987911016 82-83 0.04813709296870294 84-85 0.04978635874556572 86-87 0.05776860065114649 88-89 0.06435032967596152 90-91 0.06017264405109834 92-93 0.06475412718537937 94-95 0.07320150293092248 96-97 0.07213322850727272 98-99 0.04739253806737128 100 0.006787887246922893 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2.9346392E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 39.72709975212825 #Duplication Level Percentage of deduplicated Percentage of total 1 66.10546684320921 26.26178475441179 2 17.75161792238286 14.10440591928343 3 6.37503722147072 7.597852188626933 4 2.906988519410779 4.619448915556945 5 1.5754576756041638 3.129418211699136 6 1.0347841598347907 2.466538412380737 7 0.7212153434938584 2.005625572562217 8 0.54361892199617 1.7277122513029015 9 0.4267204490813293 1.525712926223424 >10 2.227384222870636 16.010826355590105 >50 0.1892683987914034 5.305097035540088 >100 0.1400751705161907 9.584771694373435 >500 0.0017863838768663433 0.41397846803046257 >1k 5.09963359337331E-4 0.3717864418861121 >5k 3.440205076469887E-5 0.08775540262778314 >10k+ 3.440205075395605E-5 4.787285449904507 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 1307812 4.4564660623357035 No Hit AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 47257 0.16103172069670438 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 5.792875662534598E-4 1.0222721757413995E-5 0.0 1.3630295676551993E-5 1.703786959568999E-5 2 5.895102880108737E-4 2.044544351482799E-5 0.0 1.3630295676551993E-5 1.703786959568999E-5 3 6.235860272022537E-4 3.407573919137998E-5 0.0 1.3630295676551993E-5 1.703786959568999E-5 4 6.542541924744957E-4 4.089088702965598E-5 0.0 2.044544351482799E-5 2.044544351482799E-5 5 6.576617663936337E-4 5.1113608787069974E-5 0.0 2.044544351482799E-5 2.044544351482799E-5 6 6.781072099084617E-4 5.1113608787069974E-5 0.0 2.3853017433965987E-5 2.044544351482799E-5 7 6.849223577467376E-4 6.133633054448397E-5 0.0 2.3853017433965987E-5 2.7260591353103987E-5 8 6.849223577467376E-4 6.474390446362197E-5 3.4075739191379983E-6 2.7260591353103987E-5 2.7260591353103987E-5 9 6.883299316658756E-4 7.837420014017396E-5 3.4075739191379983E-6 2.7260591353103987E-5 2.7260591353103987E-5 10-11 7.207018838976866E-4 8.178177405931196E-5 3.4075739191379983E-6 2.7260591353103987E-5 2.7260591353103987E-5 12-13 7.445549013316527E-4 9.030070885715695E-5 3.4075739191379983E-6 2.7260591353103987E-5 3.577952615094898E-5 14-15 7.667041318060496E-4 9.200449581672596E-5 3.4075739191379983E-6 3.407573919137998E-5 3.748331311051798E-5 16-17 8.041874449165676E-4 9.541206973586396E-5 3.4075739191379983E-6 4.7706034867931974E-5 5.1113608787069974E-5 18-19 8.570048406632066E-4 1.1244993933155395E-4 3.4075739191379983E-6 4.940982182750098E-5 6.133633054448398E-5 20-21 8.978957276928626E-4 1.2096887412939894E-4 3.4075739191379983E-6 5.452118270620797E-5 8.007798709974297E-5 22-23 9.864926495904505E-4 1.2608023500810595E-4 3.4075739191379983E-6 5.792875662534597E-5 9.030070885715695E-5 24-25 0.0012505796283236452 1.3630295676551993E-4 3.4075739191379983E-6 5.792875662534597E-5 9.370828277629495E-5 26-27 0.0022064041126418537 1.4141431764422692E-4 3.4075739191379983E-6 6.474390446362197E-5 1.0563479149327795E-4 28-29 0.0049818730697797535 1.465256785229339E-4 3.4075739191379983E-6 6.644769142319097E-5 1.0904236541241595E-4 30-31 0.01234052894815826 1.601559741994859E-4 3.4075739191379983E-6 7.155905230189796E-5 1.2267266108896793E-4 32-33 0.02852650506406375 1.601559741994859E-4 3.4075739191379983E-6 7.496662622103595E-5 1.3800674372508893E-4 34-35 0.058758500874656075 1.6867490899733092E-4 3.4075739191379983E-6 9.200449581672596E-5 1.465256785229339E-4 36-37 0.10875953677712749 1.703786959568999E-4 3.4075739191379983E-6 9.541206973586395E-5 1.5334082636120992E-4 38-39 0.18155724219863212 1.737862698760379E-4 3.4075739191379983E-6 1.0052343061457095E-4 1.5674840028034791E-4 40-41 0.280732977328184 1.771938437951759E-4 3.4075739191379983E-6 1.0222721757413995E-4 1.5674840028034791E-4 42-43 0.4080177897167052 1.8060141771431392E-4 3.4075739191379983E-6 1.0222721757413995E-4 1.5674840028034791E-4 44-45 0.5706221057770917 1.891203525121589E-4 3.4075739191379983E-6 1.0222721757413995E-4 1.6185976115905493E-4 46-47 0.7720335774155814 1.976392873100039E-4 3.4075739191379983E-6 1.0563479149327795E-4 1.6526733507819292E-4 48-49 1.0207217296081916 2.248998786631079E-4 3.4075739191379983E-6 1.0733857845284695E-4 1.771938437951759E-4 50-51 1.3097436304946788 2.300112395418149E-4 3.4075739191379983E-6 1.0904236541241595E-4 1.840089916334519E-4 52-53 1.6339572510310636 2.4193774825879787E-4 3.4075739191379983E-6 1.1244993933155394E-4 1.9423171339086592E-4 54-55 1.995446322668899 2.623831917736259E-4 3.4075739191379983E-6 1.1244993933155394E-4 1.976392873100039E-4 56-57 2.408853190538721 2.6919833961190187E-4 3.4075739191379983E-6 1.2096887412939894E-4 2.044544351482799E-4 58-59 2.8789297164707675 2.7942106136931585E-4 3.4075739191379983E-6 1.2437644804853693E-4 2.078620090674179E-4 60-61 3.39539184237708 2.8623620920759183E-4 3.4075739191379983E-6 1.3630295676551993E-4 2.163809438652629E-4 62-63 3.9479026927739533 2.9816271792457486E-4 3.4075739191379983E-6 1.4141431764422692E-4 2.197885177844009E-4 64-65 4.533461898825586 2.9986650488414386E-4 3.4075739191379983E-6 1.4652567852293394E-4 2.2149230474396989E-4 66-67 5.160469130242655 3.0668165272241984E-4 3.4075739191379983E-6 1.4822946548250293E-4 2.2489987866310788E-4 68-69 5.831575479534248 3.0668165272241984E-4 3.4075739191379983E-6 1.6526733507819292E-4 2.300112395418149E-4 70-71 6.538440909533274 3.169043744798338E-4 3.4075739191379983E-6 1.720824829164689E-4 2.3512260042052188E-4 72-73 7.280423092555978 3.458687527925068E-4 3.4075739191379983E-6 1.737862698760379E-4 2.3853017433965988E-4 74-75 8.048278984346696 3.6290662238819683E-4 3.4075739191379983E-6 1.771938437951759E-4 2.3853017433965988E-4 76-77 8.8431518259553 3.901672137413008E-4 3.4075739191379983E-6 1.8060141771431392E-4 2.4193774825879787E-4 78-79 9.670878791505274 3.918710007008698E-4 3.4075739191379983E-6 1.874165655525899E-4 2.589756178544879E-4 80-81 10.522685378154835 4.072050833369908E-4 3.4075739191379983E-6 1.925279264312969E-4 2.657907656927639E-4 82-83 11.397113144266594 4.123164442156978E-4 3.4075739191379983E-6 1.959355003504349E-4 2.7090212657147086E-4 84-85 12.292437857437466 4.157240181348358E-4 3.4075739191379983E-6 2.010468612291419E-4 2.7601348745017785E-4 86-87 13.207594650817722 4.1913159205397377E-4 3.4075739191379983E-6 2.010468612291419E-4 2.7942106136931585E-4 88 13.904547448285976 4.225391659731118E-4 3.4075739191379983E-6 2.044544351482799E-4 2.7942106136931585E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 177700 0.0 82.50451 8 AGTGGTA 177705 0.0 81.813644 5 AAGCAGT 172855 0.0 81.70279 1 TGGTATC 179845 0.0 81.446625 7 GTATCAA 180590 0.0 81.108536 9 GTGGTAT 181125 0.0 80.336235 6 GCAGTGG 181080 0.0 80.26334 3 CAGTGGT 188140 0.0 77.14947 4 AGCAGTG 188415 0.0 76.81586 2 CAACGCA 172975 0.0 44.150974 12-13 ATCAACG 174755 0.0 43.867798 10-11 ACGCAGA 176160 0.0 43.423363 14-15 AGAGTAC 179055 0.0 42.65342 18-19 TCAACGC 172760 0.0 42.30129 12-13 AACGCAG 174405 0.0 41.958855 14-15 GAGTACT 173610 0.0 41.773506 20-21 AGTACTT 181990 0.0 41.632267 20-21 CGCAGAG 177200 0.0 41.291836 16-17 TATCAAC 180135 0.0 40.64628 10-11 GTACTTT 183480 0.0 39.49459 22-23 >>END_MODULE