FastQCFastQC Report
Thu 26 May 2016
SRR1660729_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660729_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27379562
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT14007985.116217710129914No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT366450.13384070935831624No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1898800.081.6589361
GGTATCA1886150.081.639168
AGTGGTA1890750.081.590035
GTGGTAT1896600.081.335366
TGGTATC1898950.080.861457
GCAGTGG1937950.080.052393
GTATCAA1922000.079.989149
CAGTGGT1966750.078.370274
AGCAGTG1998750.077.65212
GAGTACT1757150.042.81318320-21
CAACGCA1869400.042.6239912-13
ACGCAGA1875500.042.48810614-15
ATCAACG1881850.042.480110-11
AGAGTAC1853300.042.3626318-19
AGTACTT1837700.042.20931620-21
TCAACGC1863450.041.3701912-13
AACGCAG1866400.041.3603314-15
CGCAGAG1888000.040.83250416-17
GTACTTT1849750.040.6406922-23
TATCAAC1923400.039.9349710-11
Produced by FastQC (version 0.11.5)