FastQCFastQC Report
Thu 26 May 2016
SRR1660729_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660729_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27379562
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT14594095.330286145556309No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT385800.14090802475218558No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1947200.083.011311
GGTATCA2022900.082.0148858
AGTGGTA2015250.081.891065
GCAGTGG2013250.081.884433
GTGGTAT2021800.081.616486
TGGTATC2042500.081.2209857
GTATCAA2063250.080.290179
AGCAGTG2071000.079.443482
CAGTGGT2096700.078.551434
GAGTACT1915800.042.728220-21
CAACGCA2002900.042.68019512-13
ACGCAGA2012650.042.57726714-15
ATCAACG2015900.042.5341410-11
AGAGTAC2012950.042.47141618-19
AGTACTT1995150.042.3666820-21
TCAACGC1996750.041.36454412-13
AACGCAG2000150.041.3623614-15
CGCAGAG2024750.040.8844816-17
GTACTTT2008800.040.7270822-23
TATCAAC2060900.040.13733710-11
Produced by FastQC (version 0.11.5)