##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660729_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 27379562 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.43625069677886 34.0 31.0 34.0 28.0 34.0 2 32.018737699310165 34.0 31.0 34.0 30.0 34.0 3 31.84835882327117 34.0 31.0 34.0 30.0 34.0 4 35.68218198669504 37.0 35.0 37.0 35.0 37.0 5 35.94324160481457 37.0 35.0 37.0 35.0 37.0 6 35.50148965129537 37.0 35.0 37.0 35.0 37.0 7 35.65667796292724 37.0 35.0 37.0 35.0 37.0 8 35.96809423028754 37.0 35.0 37.0 35.0 37.0 9 37.77004635793662 39.0 38.0 39.0 35.0 39.0 10-11 37.76419909493073 39.0 37.5 39.0 35.0 39.0 12-13 37.8442722348882 39.0 38.0 39.0 35.0 39.0 14-15 39.294241010867886 41.0 39.0 41.0 36.0 41.0 16-17 39.26537150229065 41.0 39.0 41.0 36.0 41.0 18-19 39.2612572655472 41.0 39.0 41.0 36.0 41.0 20-21 39.19742764694337 40.5 39.0 41.0 36.0 41.0 22-23 39.06691049330884 40.0 38.5 41.0 35.5 41.0 24-25 39.08217110996881 40.0 39.0 41.0 36.0 41.0 26-27 39.03610278718118 40.0 39.0 41.0 35.0 41.0 28-29 38.96002788503337 40.0 39.0 41.0 35.0 41.0 30-31 38.858798946455025 40.0 38.0 41.0 35.0 41.0 32-33 38.730455731906886 40.0 38.0 41.0 35.0 41.0 34-35 38.62951752113493 40.0 38.0 41.0 34.5 41.0 36-37 38.485441476383 40.0 38.0 41.0 34.0 41.0 38-39 37.89865838613488 40.0 37.0 41.0 33.5 41.0 40-41 37.77569699617547 40.0 36.5 41.0 33.0 41.0 42-43 37.75577109305109 40.0 36.0 41.0 33.0 41.0 44-45 37.64822333534773 40.0 36.0 41.0 33.0 41.0 46-47 37.49727050052883 40.0 36.0 41.0 33.0 41.0 48-49 37.49230376658326 40.0 36.0 41.0 33.0 41.0 50-51 37.444000875543594 40.0 36.0 41.0 33.0 41.0 52-53 37.16595009810602 40.0 35.0 41.0 31.5 41.0 54-55 36.624969785857054 39.5 35.0 41.0 30.5 41.0 56-57 36.12306405778149 39.0 35.0 41.0 28.0 41.0 58-59 35.97302918870652 39.0 35.0 41.0 28.0 41.0 60-61 35.836327093179946 39.0 35.0 41.0 28.0 41.0 62-63 35.61288593659752 39.0 35.0 41.0 27.0 41.0 64-65 35.36119126010854 38.0 35.0 40.5 27.0 41.0 66-67 35.096480597461714 37.0 35.0 40.0 26.0 41.0 68-69 34.83730269315484 37.0 34.5 40.0 26.0 41.0 70-71 34.406868123018185 36.0 34.0 39.0 25.5 41.0 72-73 34.04601708018558 36.0 34.0 39.0 25.5 41.0 74-75 33.79541707058718 35.0 34.0 38.5 26.0 40.0 76-77 32.12244286449871 34.5 31.5 36.5 23.0 39.0 78-79 33.05117393769849 35.0 33.5 37.0 25.0 39.0 80-81 32.95942975640005 35.0 34.0 36.5 25.0 39.0 82-83 32.70979544888264 35.0 34.0 36.0 25.0 38.0 84-85 32.47154152794701 35.0 34.0 36.0 24.0 37.0 86-87 32.26390120849997 35.0 33.0 35.0 24.0 37.0 88-89 32.09561633966241 35.0 33.0 35.0 24.0 36.0 90-91 31.942343781832594 35.0 33.0 35.0 23.5 36.0 92-93 31.807677383589994 35.0 33.0 35.0 23.0 36.0 94-95 31.70493691608361 35.0 33.0 35.0 22.5 36.0 96-97 31.609376055760134 35.0 33.0 35.0 20.0 35.0 98-99 31.527000833687552 35.0 33.0 35.0 20.0 35.0 100 31.476353931447115 35.0 33.0 35.0 20.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.3214638189370653 1101 2 1.002751693954096 1101 3 1.2927749724765114 1101 4 0.8071949422893141 1101 5 0.4883670753670586 1101 6 0.9391183402673349 1101 7 0.7105853286214696 1101 8 0.4447489662586719 1101 9 0.4748480472192256 1101 10-11 0.3997182526729972 1101 12-13 0.36225127586289574 1101 14-15 0.4784625615363467 1101 16-17 0.4913565124425858 1101 18-19 0.46052807687721753 1101 20-21 0.4618070328309756 1101 22-23 0.46714947906171744 1101 24-25 0.4622523709569464 1101 26-27 0.4500037668051746 1101 28-29 0.4579095870984773 1101 30-31 0.44360330139047477 1101 32-33 0.4830869029118432 1101 34-35 0.46339099416995566 1101 36-37 0.42576521728616257 1101 38-39 0.6773461407842731 1101 40-41 0.655885866740185 1101 42-43 0.5194156023289196 1101 44-45 0.48367799993256 1101 46-47 0.45781052399788535 1101 48-49 0.452898469106529 1101 50-51 0.4501833949806979 1101 52-53 0.41653084628551085 1101 54-55 0.4630060792645665 1101 56-57 0.4885391899064473 1101 58-59 0.5130377875143424 1101 60-61 0.5262472312494495 1101 62-63 0.5116073260047145 1101 64-65 0.47811412334787207 1101 66-67 0.4832632872359923 1101 68-69 0.4332526011055293 1101 70-71 0.6119356595690206 1101 72-73 0.6244796661221628 1101 74-75 0.4882488296300025 1101 76-77 0.3910252341222673 1101 78-79 0.4735053072283506 1101 80-81 0.45687403193542053 1101 82-83 0.4588920733857904 1101 84-85 0.448989240501497 1101 86-87 0.4374927678573428 1101 88-89 0.4390855801443223 1101 90-91 0.4183029872498878 1101 92-93 0.38843978443827964 1101 94-95 0.36263938827750053 1101 96-97 0.37991524786149355 1101 98-99 0.3403904947167362 1101 100 0.35999481815123957 1102 1 1.2733897269460108 1102 2 0.9668753201752196 1102 3 1.2545498651297642 1102 4 0.76552430633987 1102 5 0.4439884234756448 1102 6 0.8889766926306777 1102 7 0.6707071746753144 1102 8 0.41329573668594577 1102 9 0.4312989764547126 1102 10-11 0.364156068269601 1102 12-13 0.35596537176061105 1102 14-15 0.46388721110911746 1102 16-17 0.45382356598953777 1102 18-19 0.398039183657275 1102 20-21 0.38634539964387216 1102 22-23 0.4013874197357836 1102 24-25 0.41062204298997074 1102 26-27 0.43549346866590355 1102 28-29 0.443692509564265 1102 30-31 0.452802624768573 1102 32-33 0.4428591301217679 1102 34-35 0.4153211173548357 1102 36-37 0.4126647338861389 1102 38-39 0.599600011149434 1102 40-41 0.6018111841240241 1102 42-43 0.4549295887025835 1102 44-45 0.41493668515471427 1102 46-47 0.4105151836285401 1102 48-49 0.4049077848974818 1102 50-51 0.374003804629794 1102 52-53 0.41370928694457376 1102 54-55 0.4182590312332195 1102 56-57 0.4168348102366579 1102 58-59 0.3739148213421899 1102 60-61 0.3814628951670258 1102 62-63 0.36311274220436474 1102 64-65 0.3438725384945016 1102 66-67 0.34236853579241 1102 68-69 0.32551889278943946 1102 70-71 0.45791841140272993 1102 72-73 0.459793147941447 1102 74-75 0.335524178861597 1102 76-77 0.14841612143056437 1102 78-79 0.3728080138105412 1102 80-81 0.3442121611462383 1102 82-83 0.3363974303132764 1102 84-85 0.34386612988538445 1102 86-87 0.3318992807631176 1102 88-89 0.3006245122343998 1102 90-91 0.299342750717301 1102 92-93 0.2668946232527709 1102 94-95 0.26184157329277014 1102 96-97 0.28235525766617897 1102 98-99 0.28546599395854244 1102 100 0.24009860782075876 1103 1 1.2393714746273368 1103 2 0.9170872685607918 1103 3 1.206378008970571 1103 4 0.7598202591379746 1103 5 0.44774928966489114 1103 6 0.8821085387891046 1103 7 0.657414296105209 1103 8 0.40334913728887756 1103 9 0.3960235287888594 1103 10-11 0.3364733105529041 1103 12-13 0.2906413681860087 1103 14-15 0.367097859216571 1103 16-17 0.39573066866906714 1103 18-19 0.3588456724167628 1103 20-21 0.38575687763058397 1103 22-23 0.3357438683633802 1103 24-25 0.3561925190284754 1103 26-27 0.38935912450602217 1103 28-29 0.36157221186982014 1103 30-31 0.36544796572677996 1103 32-33 0.3935949206595737 1103 34-35 0.40153978169426807 1103 36-37 0.35473748587025256 1103 38-39 0.5540774554325267 1103 40-41 0.5697180347826816 1103 42-43 0.4344987756991898 1103 44-45 0.38126685367712554 1103 46-47 0.36224916447772415 1103 48-49 0.3750895696487717 1103 50-51 0.3639206871532892 1103 52-53 0.3563651347019885 1103 54-55 0.3642900595008669 1103 56-57 0.3841306142831016 1103 58-59 0.39227613608952794 1103 60-61 0.3698400190970048 1103 62-63 0.35302728782836823 1103 64-65 0.32388427484061566 1103 66-67 0.337262032821954 1103 68-69 0.328431303535929 1103 70-71 0.4231775955919801 1103 72-73 0.44609259490918163 1103 74-75 0.3231964574717381 1103 76-77 0.17407582710048075 1103 78-79 0.34252406766434973 1103 80-81 0.3681019443994913 1103 82-83 0.33162449565347174 1103 84-85 0.29759959838151673 1103 86-87 0.27182523554960625 1103 88-89 0.25032630387467947 1103 90-91 0.3114217085798714 1103 92-93 0.3272118342217958 1103 94-95 0.3037783720144702 1103 96-97 0.35262943332660157 1103 98-99 0.34028544768441904 1103 100 0.33015435398820614 1104 1 1.207757837250913 1104 2 0.8783580385003589 1104 3 1.1792703377277256 1104 4 0.7241975279084727 1104 5 0.41097909415113776 1104 6 0.8310120778247381 1104 7 0.6130719310883848 1104 8 0.3435443366935189 1104 9 0.349018146515057 1104 10-11 0.28029806335001695 1104 12-13 0.25144073885505946 1104 14-15 0.32613353219768726 1104 16-17 0.3511940815968231 1104 18-19 0.306759251312144 1104 20-21 0.29769656224513596 1104 22-23 0.2894131982344703 1104 24-25 0.2895095156495131 1104 26-27 0.29836132284894035 1104 28-29 0.27881244731641885 1104 30-31 0.2840377288985181 1104 32-33 0.29790565468322683 1104 34-35 0.33271176452640816 1104 36-37 0.30889786198407876 1104 38-39 0.5274006162757061 1104 40-41 0.48767795623340504 1104 42-43 0.33961312695528534 1104 44-45 0.31229539546979623 1104 46-47 0.33961111004814626 1104 48-49 0.30448257919612587 1104 50-51 0.30767715455186817 1104 52-53 0.30937243393184133 1104 54-55 0.3186077591572811 1104 56-57 0.3773508589777421 1104 58-59 0.37859740637400563 1104 60-61 0.3629091529380588 1104 62-63 0.3521857172066021 1104 64-65 0.3071381579953467 1104 66-67 0.2900540567958174 1104 68-69 0.27996142184932893 1104 70-71 0.4418632420301023 1104 72-73 0.44471156701197145 1104 74-75 0.33451804306988464 1104 76-77 0.2335610772820047 1104 78-79 0.33309822073914575 1104 80-81 0.35029933138650193 1104 82-83 0.3560428860876712 1104 84-85 0.30515704804759736 1104 86-87 0.3146057725833131 1104 88-89 0.31117673746209107 1104 90-91 0.30563817371685786 1104 92-93 0.3231748448369025 1104 94-95 0.3098726053587768 1104 96-97 0.33228635754137414 1104 98-99 0.3153089438476364 1104 100 0.3307360646087112 1105 1 1.1376234820721542 1105 2 0.8461555262522822 1105 3 1.1214165012495094 1105 4 0.6964116603871631 1105 5 0.3685824400830171 1105 6 0.8419858542103924 1105 7 0.615019158013709 1105 8 0.3342807245788393 1105 9 0.31265367684712686 1105 10-11 0.24602980201701996 1105 12-13 0.2187767082344081 1105 14-15 0.30661718756168455 1105 16-17 0.30207463941507484 1105 18-19 0.2643161222499444 1105 20-21 0.2926405735063824 1105 22-23 0.30196066433832414 1105 24-25 0.27862635763349886 1105 26-27 0.2986775780253552 1105 28-29 0.27110889885565825 1105 30-31 0.28582380904938276 1105 32-33 0.33975502776517175 1105 34-35 0.3436851948363113 1105 36-37 0.29228207219643565 1105 38-39 0.5025304553034076 1105 40-41 0.49952405284125945 1105 42-43 0.3382493501340633 1105 44-45 0.3101901990968443 1105 46-47 0.3156333577349173 1105 48-49 0.2736935426919942 1105 50-51 0.28052083855028087 1105 52-53 0.29023181296831524 1105 54-55 0.2991550061317767 1105 56-57 0.3139108571675777 1105 58-59 0.305409644694997 1105 60-61 0.2622544734156449 1105 62-63 0.25517787089616206 1105 64-65 0.24295430646353822 1105 66-67 0.2725535071580012 1105 68-69 0.22224306295069596 1105 70-71 0.3675354999470528 1105 72-73 0.35495251951194007 1105 74-75 0.24370995134452755 1105 76-77 0.12325177275823762 1105 78-79 0.23073182075080467 1105 80-81 0.2647652408446177 1105 82-83 0.2676266331339434 1105 84-85 0.2472037541347234 1105 86-87 0.23833753562687576 1105 88-89 0.2468442093423704 1105 90-91 0.23011778659826376 1105 92-93 0.22572474580140423 1105 94-95 0.22849503052022868 1105 96-97 0.24359472913200975 1105 98-99 0.21982529918676974 1105 100 0.20546370738437858 1106 1 1.112188794064462 1106 2 0.7963939096629957 1106 3 1.0820163812184482 1106 4 0.6394239940108974 1106 5 0.3553138975902286 1106 6 0.7920483161529646 1106 7 0.5685842264513639 1106 8 0.2906549910690899 1106 9 0.283330330390946 1106 10-11 0.2001049056131592 1106 12-13 0.18159539300256 1106 14-15 0.2132601214993457 1106 16-17 0.21538569407061203 1106 18-19 0.20326021259434413 1106 20-21 0.21323772421648357 1106 22-23 0.218434413320864 1106 24-25 0.21609891985092133 1106 26-27 0.1872300377403988 1106 28-29 0.20681359299285873 1106 30-31 0.21099423672435336 1106 32-33 0.24356956834442656 1106 34-35 0.24438842180567377 1106 36-37 0.2318420871092144 1106 38-39 0.4640451459204158 1106 40-41 0.44458824271161035 1106 42-43 0.32618630558457085 1106 44-45 0.2748671580185871 1106 46-47 0.30367750425064344 1106 48-49 0.2528542197606285 1106 50-51 0.25823961176594423 1106 52-53 0.25649400007363 1106 54-55 0.3046893550464631 1106 56-57 0.33432069174319423 1106 58-59 0.320713870313476 1106 60-61 0.29773914777525334 1106 62-63 0.27954820514359113 1106 64-65 0.25104504566343877 1106 66-67 0.2356743174906839 1106 68-69 0.23152092252212952 1106 70-71 0.36681903340613076 1106 72-73 0.34754516650894374 1106 74-75 0.2475181223188727 1106 76-77 0.1264181054298703 1106 78-79 0.2615541566156665 1106 80-81 0.3168561385305253 1106 82-83 0.3085836623038958 1106 84-85 0.2689656097253632 1106 86-87 0.28482885867172314 1106 88-89 0.2681113873058223 1106 90-91 0.2764840699599631 1106 92-93 0.28014625697575823 1106 94-95 0.24343490701823 1106 96-97 0.2686184250129422 1106 98-99 0.29181833297912263 1106 100 0.2622309000834804 1107 1 1.045119947525098 1107 2 0.7426476350258042 1107 3 1.0343264150619653 1107 4 0.6458671887438214 1107 5 0.3083667440517104 1107 6 0.7690477428494731 1107 7 0.5350793870434742 1107 8 0.2367103522457441 1107 9 0.22736891545994808 1107 10-11 0.1716274853355344 1107 12-13 0.12127785237613864 1107 14-15 0.17898243964063454 1107 16-17 0.1685668344396234 1107 18-19 0.12953255133187724 1107 20-21 0.1159228912696264 1107 22-23 0.17075657396683397 1107 24-25 0.16790226637501604 1107 26-27 0.14388397152078625 1107 28-29 0.13582198160832348 1107 30-31 0.10525746149821913 1107 32-33 0.15378984314926214 1107 34-35 0.18009428154186935 1107 36-37 0.17142020262564728 1107 38-39 0.36206421732814675 1107 40-41 0.3285017447310352 1107 42-43 0.2056970508529048 1107 44-45 0.19526507426524375 1107 46-47 0.19994977853298934 1107 48-49 0.196097481291595 1107 50-51 0.15170495084116453 1107 52-53 0.16762294259827115 1107 54-55 0.1484515276495415 1107 56-57 0.1478981257462948 1107 58-59 0.14748111257122076 1107 60-61 0.15413633126537718 1107 62-63 0.12872699723089198 1107 64-65 0.12070004164394987 1107 66-67 0.13888852256912543 1107 68-69 0.1252273540908604 1107 70-71 0.2715716173504603 1107 72-73 0.25806884271838015 1107 74-75 0.14218094987225527 1107 76-77 -0.014956307994481222 1107 78-79 0.12515766421354613 1107 80-81 0.17153759376714817 1107 82-83 0.1575171469027694 1107 84-85 0.16055732338771378 1107 86-87 0.16519476850432113 1107 88-89 0.16232491472219834 1107 90-91 0.16335867276489324 1107 92-93 0.15893848602286553 1107 94-95 0.11441382862034288 1107 96-97 0.14054564959552351 1107 98-99 0.15146582514541151 1107 100 0.1966045499679261 1108 1 1.0001781283080753 1108 2 0.7140880988937326 1108 3 1.0008888179786162 1108 4 0.6030627670312256 1108 5 0.29670261520278984 1108 6 0.7457447540572701 1108 7 0.5146790392236511 1108 8 0.22933239405685413 1108 9 0.1803758344060924 1108 10-11 0.11215813626957072 1108 12-13 0.1199447990029654 1108 14-15 0.11585833005140955 1108 16-17 0.12899847172595003 1108 18-19 0.09187429649572465 1108 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2316 70-71 -0.5097217374950205 2316 72-73 -0.5324994725106507 2316 74-75 -0.6825681189945456 2316 76-77 -0.6048800435587296 2316 78-79 -0.8484220362120425 2316 80-81 -1.006385650160393 2316 82-83 -1.0955080235603134 2316 84-85 -0.8429868194331576 2316 86-87 -0.7025892529381679 2316 88-89 -0.5913365334678389 2316 90-91 -0.662024026320637 2316 92-93 -0.7125216081818557 2316 94-95 -0.7254830420755596 2316 96-97 -0.7933402030114678 2316 98-99 -0.9151276072928134 2316 100 -0.8606897755968177 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 45.0 7 731.0 8 984.0 9 412.0 10 342.0 11 629.0 12 896.0 13 1555.0 14 4367.0 15 12060.0 16 25791.0 17 37914.0 18 43092.0 19 51910.0 20 69631.0 21 98866.0 22 147006.0 23 247280.0 24 395950.0 25 470238.0 26 459042.0 27 385849.0 28 342074.0 29 357405.0 30 408264.0 31 494259.0 32 608169.0 33 777923.0 34 1069510.0 35 1666703.0 36 3013892.0 37 5887590.0 38 7954424.0 39 2339048.0 40 5711.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 16.316466439996162 32.873687713066836 27.649190608407466 23.160655238529536 2 21.07274773034876 33.73132289469998 27.151071997130554 18.04485737782071 3 25.388992509131274 25.694822990802173 27.254067467549792 21.662117032516758 4 19.101634847159975 28.1105791332959 25.14269589533057 27.645090124213556 5 21.5707539126004 32.81416975653328 26.258726722090753 19.35634960877557 6 28.553313639354137 27.362404525865188 25.557481868735476 18.5267999660452 7 20.742251180589506 26.207688054955963 32.672893063034024 20.377167701420507 8 26.74750040112138 26.684434952831303 26.058806129098155 20.509258516949163 9 26.126038831446607 25.1634558653641 27.80814755181255 20.902357751376737 10-11 20.412322335465703 29.928671216290994 29.234160166985944 20.424846281257363 12-13 20.31295363463046 26.823524242599888 29.121425329436555 23.742096793333097 14-15 20.29947374614685 33.215213596185365 25.99509992161306 20.490212736054726 16-17 23.688392575291495 26.552586523235057 26.05294542244718 23.706075479026264 18-19 20.35670073904031 29.881659538600363 26.101348882060275 23.66029084029905 20-21 23.604410382445103 29.97635455889693 26.06764882538879 20.351586233269177 22-23 23.544267805380205 26.784796691385342 29.281856984996217 20.389078518238232 24-25 20.42049439474423 29.814173501555686 25.95936457788806 23.805967525812026 26-27 20.504558495838822 26.631105168793574 32.496551707512914 20.367784627854693 28-29 20.465330666343977 26.742782493230834 32.464998504101096 20.326888336324096 30-31 20.412399194492817 26.624548362308463 32.5008911718098 20.462161271388922 32-33 20.55864232717404 26.769227757062453 32.28602104920639 20.386108866557116 34-35 20.551829251198715 26.854954459000407 32.195600208179286 20.397616081621592 36-37 20.47113829118066 26.802265560166425 32.284703546075356 20.441892602577553 38-39 20.519084473284206 26.805839544776074 32.27962998317801 20.39544599876171 40-41 20.558672710074262 26.820299184252473 32.2418499486681 20.379178157005164 42-43 20.54961091492968 26.880771749391783 32.18623101329824 20.383386322380296 44-45 20.623187297871297 26.96124745964824 32.102182352936346 20.313382889544123 46-47 20.62438067819919 27.106480505142088 31.978544127258846 20.29059468939987 48-49 20.672418475947616 27.1277384367494 31.859011742292854 20.34083134501013 50-51 20.708775455656646 27.192933147949795 31.743623616631595 20.35466777976197 52-53 20.829624872502674 27.309897633234126 31.451451508516083 20.409025985747125 54-55 21.154148865912585 27.549839569209162 30.728845740616258 20.567165824261995 56-57 21.61555133477536 28.08233928626031 29.526017449363394 20.77609192960093 58-59 21.73607932686187 28.64892689413435 28.748697726069622 20.866296052934157 60-61 21.848709303912347 28.929347577615864 28.29101396308433 20.93092915538746 62-63 21.850793222615607 29.133020405231758 27.991582227248372 21.02460414490426 64-65 21.846489269447886 29.227227277480516 27.86418741122667 21.062096041844928 66-67 21.71467360237065 29.151923405695385 27.99179786236981 21.141605129564155 68-69 21.622490648350293 29.049148632709166 28.07969125009027 21.248669468850263 70-71 21.638295479167564 28.887235846634358 28.10017638431017 21.374292289887908 72-73 21.460702442260622 28.79091399433741 28.1214059150734 21.62697764832857 74-75 21.521757401835984 28.78509433707154 28.023308461083925 21.66983980000855 76-77 21.55418036622157 28.849713184564195 27.87189275969174 21.724213689522493 78-79 21.602597698967404 28.995459014792484 27.567753240523306 21.834190045716806 80-81 21.641033670753114 29.139813874805498 27.225266370456392 21.993886083984997 82-83 21.54790873286091 29.22557846399797 26.930866010523317 22.2956467926178 84-85 21.47004094165664 29.198491176225982 26.79343559563599 22.53803228648139 86-87 21.417023252261615 29.13642548574605 26.74376248521599 22.70278877677635 88-89 21.34931738426697 29.067575792879257 26.667687821466423 22.915419001387345 90-91 21.29033218759635 28.997647166299345 26.579590046933344 23.13243059917096 92-93 21.218168741777603 28.940033092584578 26.537652434896593 23.304145730741226 94-95 21.19984713018721 28.82741672400503 26.499760946769563 23.472975199038196 96-97 21.172807902310826 28.683074460142898 26.543026078073765 23.601091559472508 98-99 21.131172347148176 28.61190852808097 26.627139232802865 23.629779891967985 100 21.042794782793546 28.525381996481098 26.667315609285897 23.76450761143946 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 20646.0 1 15531.0 2 8501.0 3 6171.5 4 5703.0 5 5656.0 6 5771.0 7 5892.5 8 5988.0 9 6257.5 10 7123.5 11 13387.5 12 107744.5 13 128366.0 14 58093.5 15 60174.0 16 68743.5 17 76693.0 18 84400.0 19 93032.5 20 104011.5 21 121166.0 22 145554.5 23 173745.0 24 202013.5 25 234501.5 26 271424.5 27 313119.0 28 365449.0 29 418042.5 30 461066.5 31 503147.5 32 550713.0 33 591996.0 34 627140.5 35 671696.0 36 717421.5 37 756068.5 38 799862.5 39 833047.0 40 850054.5 41 878987.0 42 913739.0 43 919051.5 44 907110.5 45 910027.5 46 917052.0 47 916033.5 48 907552.0 49 896809.0 50 888140.0 51 880179.0 52 864201.5 53 823388.0 54 765893.0 55 709675.0 56 652213.5 57 586832.0 58 516060.0 59 439043.5 60 358932.5 61 290535.0 62 229709.5 63 174496.0 64 131722.5 65 98359.5 66 73108.5 67 55735.0 68 43031.0 69 33184.5 70 25773.0 71 20546.5 72 16896.0 73 13605.5 74 10832.5 75 8800.0 76 6569.5 77 4185.0 78 2501.5 79 1570.0 80 966.5 81 632.5 82 404.5 83 225.5 84 139.0 85 98.5 86 70.5 87 47.0 88 28.5 89 20.5 90 15.5 91 11.5 92 7.0 93 7.5 94 6.0 95 2.5 96 1.0 97 1.0 98 1.5 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2997966147157504 2 0.005321487611817896 3 1.9550312747881067 4 6.683817659318289E-4 5 3.287123439008995E-5 6 0.007268195159586556 7 0.006260143971623797 8 2.520127969906896E-4 9 0.0 10-11 4.382831252011994E-5 12-13 4.0175953143443276E-5 14-15 0.0 16-17 3.287123439008995E-5 18-19 0.0 20-21 2.812316720041029E-4 22-23 4.63849640837936E-4 24-25 4.547187423962443E-4 26-27 0.002899973345081269 28-29 0.005021994142930409 30-31 0.004687803259964495 32-33 0.012502026146364212 34-35 0.00802971208962364 36-37 0.0042641295722700015 38-39 0.5012242343394683 40-41 0.006760517206228499 42-43 0.015524353530564148 44-45 0.027810890473704435 46-47 0.02696354309831545 48-49 0.03260826451496923 50-51 0.035203265852098 52-53 0.053954478891955975 54-55 0.030729125615669088 56-57 0.023846254370321923 58-59 0.01946342311830993 60-61 0.020568261829754617 62-63 0.022966035760542847 64-65 0.03908024533044027 66-67 0.03960253272130504 68-69 0.041134697479820896 70-71 0.13407993889748857 72-73 0.056942108862077484 74-75 0.05256475614913051 76-77 0.05451511605627584 78-79 0.050418995015332965 80-81 0.04859281532699464 82-83 0.04611286331023119 84-85 0.04680133305273474 86-87 0.05367872575901689 88-89 0.05521636905659776 90-91 0.04976157032753117 92-93 0.053028605789968444 94-95 0.059933391191575675 96-97 0.05962659300393483 98-99 0.03885379904908632 100 0.006179792065336911 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2.7379562E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.08065723080553 #Duplication Level Percentage of deduplicated Percentage of total 1 65.49555555123979 26.25104912190424 2 18.214544379477115 14.60101819778235 3 6.6701913919572435 8.020369645347236 4 3.0351290196731955 4.865998635551686 5 1.6165036802359602 3.2395264959936587 6 1.0402784433611947 2.501702622777359 7 0.7040806333137032 1.9754010168686509 8 0.503596723681131 1.614759013153607 9 0.3837928260572535 1.384440183795865 >10 2.020954999418539 15.050053785107092 >50 0.17877189052549802 5.008269778384695 >100 0.13522310926033193 9.035964177656451 >500 6.260250583028039E-4 0.16215821382645476 >1k 6.873183774732831E-4 0.549930880545284 >5k 2.7432155679154034E-5 0.07198418832576199 >10k+ 3.6576207569200174E-5 5.667374042979578 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 1459409 5.330286145556309 No Hit AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 38580 0.14090802475218558 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.003086243673291779 0.0 0.0 0.0 5.843775002682658E-5 2 0.003097200751421809 1.0957078130029983E-5 0.0 0.0 5.843775002682658E-5 3 0.0031081578295518385 2.1914156260059966E-5 0.0 0.0 5.843775002682658E-5 4 0.003111810188928515 5.113303127347326E-5 0.0 0.0 5.843775002682658E-5 5 0.003111810188928515 7.304718753353322E-5 0.0 0.0 5.843775002682658E-5 6 0.003115462548305192 9.861370317026985E-5 0.0 0.0 5.843775002682658E-5 7 0.0031191149076818686 9.861370317026985E-5 0.0 0.0 6.209010940350323E-5 8 0.003126419626435222 1.0957078130029983E-4 0.0 0.0 6.209010940350323E-5 9 0.0031300719858118986 1.1687550005365316E-4 0.0 0.0 6.57424687801799E-5 10-11 0.0031556385014486353 1.2783257818368315E-4 0.0 0.0 7.669954691020989E-5 12-13 0.0032232071499171534 1.424420156903898E-4 0.0 1.8261796883383306E-6 8.035190628688655E-5 14-15 0.0033218208530874233 1.7166089070380308E-4 0.0 1.0957078130029983E-5 8.035190628688655E-5 16-17 0.003475219946907843 1.8079178914549474E-4 3.652359376676661E-6 1.0957078130029983E-5 8.400426566356321E-5 18-19 0.0037162756657685026 2.027059454055547E-4 7.304718753353322E-6 1.0957078130029983E-5 9.130898441691653E-5 20-21 0.0039682884627591924 2.26446281353953E-4 7.304718753353322E-6 1.0957078130029983E-5 9.861370317026985E-5 22-23 0.004618408431807638 2.520127969906896E-4 7.304718753353322E-6 1.0957078130029983E-5 1.0226606254694652E-4 24-25 0.006981484948517438 2.7392695325074957E-4 7.304718753353322E-6 1.0957078130029983E-5 1.0591842192362318E-4 26-27 0.013727392717239231 2.958411095108095E-4 1.2783257818368315E-5 1.4609437506706645E-5 1.0591842192362318E-4 28-29 0.02812499338009863 3.141029063941929E-4 2.556651563673663E-5 1.4609437506706645E-5 1.132231406769765E-4 30-31 0.054197360790504974 3.341908829659145E-4 2.556651563673663E-5 1.4609437506706645E-5 1.132231406769765E-4 32-33 0.09692813931793358 3.488003204726211E-4 2.556651563673663E-5 1.4609437506706645E-5 1.2235403911866813E-4 34-35 0.1665530661155208 3.725406564210194E-4 2.556651563673663E-5 1.4609437506706645E-5 1.2418021880700647E-4 36-37 0.2680630902714952 3.871500939277261E-4 2.556651563673663E-5 1.4609437506706645E-5 1.2418021880700647E-4 38-39 0.4102202219305042 3.999333517460944E-4 2.556651563673663E-5 1.4609437506706645E-5 1.2783257818368315E-4 40-41 0.591676740482554 4.0906425018778607E-4 2.556651563673663E-5 1.4609437506706645E-5 1.314849375603598E-4 42-43 0.8120034937008853 4.2367368769449267E-4 2.556651563673663E-5 1.4609437506706645E-5 1.314849375603598E-4 44-45 1.0799624917301454 4.382831252011994E-4 2.556651563673663E-5 1.4609437506706645E-5 1.314849375603598E-4 46-47 1.405594070496818 4.6019728146125933E-4 2.556651563673663E-5 1.4609437506706645E-5 1.314849375603598E-4 48-49 1.793379674956086 4.8028525803298094E-4 2.556651563673663E-5 1.4609437506706645E-5 1.314849375603598E-4 50-51 2.2341153594787233 4.894161564746726E-4 2.556651563673663E-5 1.4609437506706645E-5 1.314849375603598E-4 52-53 2.7069370211254657 5.058517736697176E-4 2.556651563673663E-5 1.4609437506706645E-5 1.314849375603598E-4 54-55 3.219381303470085 5.387230080598076E-4 2.556651563673663E-5 1.4609437506706645E-5 1.314849375603598E-4 56-57 3.7926720668504483 5.624633440082058E-4 2.556651563673663E-5 1.8261796883383306E-5 1.314849375603598E-4 58-59 4.429628932705351 5.971607580866341E-4 2.556651563673663E-5 2.1914156260059966E-5 1.314849375603598E-4 60-61 5.110255233447489 6.044654768399874E-4 2.556651563673663E-5 2.1914156260059966E-5 1.3513729693703647E-4 62-63 5.810878201776932 6.318581721650624E-4 2.556651563673663E-5 3.287123439008995E-5 1.4609437506706645E-4 64-65 6.536654969133545 6.464676096717691E-4 2.556651563673663E-5 4.0175953143443276E-5 1.5339909382041978E-4 66-67 7.298999158569447 6.555985081134607E-4 2.556651563673663E-5 4.0175953143443276E-5 1.5522527350875811E-4 68-69 8.101953931914615 6.610770471784757E-4 2.556651563673663E-5 4.0175953143443276E-5 1.5705145319709643E-4 70-71 8.94057582075272 6.647294065551524E-4 2.556651563673663E-5 4.0175953143443276E-5 1.5705145319709643E-4 72-73 9.79850225507625 6.79338844061859E-4 2.556651563673663E-5 4.0175953143443276E-5 1.5705145319709643E-4 74-75 10.67014512503889 6.95774461256904E-4 2.556651563673663E-5 4.0175953143443276E-5 1.5705145319709643E-4 76-77 11.559122092603236 7.049053596985956E-4 2.556651563673663E-5 4.0175953143443276E-5 1.5705145319709643E-4 78-79 12.468601579528555 7.268195159586557E-4 2.556651563673663E-5 4.0175953143443276E-5 1.6252999226211141E-4 80-81 13.39767049597068 7.523860315953922E-4 2.556651563673663E-5 4.0175953143443276E-5 1.6435617195044975E-4 82-83 14.333792118369168 7.779525472321289E-4 2.556651563673663E-5 4.0175953143443276E-5 1.6435617195044975E-4 84-85 15.276838614145836 8.016928831805271E-4 2.556651563673663E-5 4.0175953143443276E-5 1.6435617195044975E-4 86-87 16.22689033520697 8.199546800639105E-4 2.739269532507496E-5 5.113303127347326E-5 1.6435617195044975E-4 88 16.943784564559508 8.327379378822787E-4 2.921887501341329E-5 5.113303127347326E-5 1.6435617195044975E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AAGCAGT 194720 0.0 83.01131 1 GGTATCA 202290 0.0 82.014885 8 AGTGGTA 201525 0.0 81.89106 5 GCAGTGG 201325 0.0 81.88443 3 GTGGTAT 202180 0.0 81.61648 6 TGGTATC 204250 0.0 81.220985 7 GTATCAA 206325 0.0 80.29017 9 AGCAGTG 207100 0.0 79.44348 2 CAGTGGT 209670 0.0 78.55143 4 GAGTACT 191580 0.0 42.7282 20-21 CAACGCA 200290 0.0 42.680195 12-13 ACGCAGA 201265 0.0 42.577267 14-15 ATCAACG 201590 0.0 42.53414 10-11 AGAGTAC 201295 0.0 42.471416 18-19 AGTACTT 199515 0.0 42.36668 20-21 TCAACGC 199675 0.0 41.364544 12-13 AACGCAG 200015 0.0 41.36236 14-15 CGCAGAG 202475 0.0 40.88448 16-17 GTACTTT 200880 0.0 40.72708 22-23 TATCAAC 206090 0.0 40.137337 10-11 >>END_MODULE