FastQCFastQC Report
Thu 26 May 2016
SRR1660728_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660728_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24318306
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT12779625.255144005507621No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT282750.11627043429752056No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA262420.10791047698799415No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1631750.085.7382058
AGTGGTA1640950.085.234345
TGGTATC1647350.084.587457
GTATCAA1655400.084.42189
GTGGTAT1665450.084.014836
GCAGTGG1715950.082.0461043
AAGCAGT1723200.081.678351
CAGTGGT1749450.080.034564
AGCAGTG1797900.078.38222
CAACGCA1605850.044.81928612-13
ATCAACG1614500.044.6485810-11
ACGCAGA1627350.044.24952314-15
AGAGTAC1626700.043.71077318-19
TCAACGC1606300.043.6215212-13
AACGCAG1621400.043.3188214-15
GAGTACT1591000.043.1598520-21
CGCAGAG1639200.042.78838316-17
TATCAAC1649450.042.30480610-11
AGTACTT1678150.042.0474220-21
GTACTTT1682100.040.82044222-23
Produced by FastQC (version 0.11.5)