##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660728_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 24318306 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.863341879158853 34.0 31.0 34.0 30.0 34.0 2 31.997488024042465 34.0 31.0 34.0 30.0 34.0 3 32.050106738520356 34.0 31.0 34.0 30.0 34.0 4 35.44792725282756 37.0 35.0 37.0 33.0 37.0 5 35.42342443589615 37.0 35.0 37.0 33.0 37.0 6 35.49311329498033 37.0 35.0 37.0 33.0 37.0 7 35.43812323934077 37.0 35.0 37.0 33.0 37.0 8 35.45798132485051 37.0 35.0 37.0 33.0 37.0 9 37.14557120055978 39.0 37.0 39.0 34.0 39.0 10-11 37.06510973667327 39.0 37.0 39.0 33.5 39.0 12-13 37.1047396763574 39.0 37.0 39.0 33.5 39.0 14-15 38.49329918375071 40.0 38.0 41.0 34.0 41.0 16-17 38.47605690544399 40.0 38.0 41.0 34.0 41.0 18-19 38.4464764527595 40.0 38.0 41.0 34.0 41.0 20-21 38.33914901391569 40.0 38.0 41.0 33.5 41.0 22-23 38.16448656004246 40.0 38.0 41.0 33.5 41.0 24-25 38.159185491785486 40.0 38.0 41.0 33.0 41.0 26-27 38.148976762608385 40.0 38.0 41.0 33.0 41.0 28-29 38.07426419833684 40.0 38.0 41.0 33.0 41.0 30-31 37.98631514464864 40.0 38.0 41.0 33.0 41.0 32-33 37.86774045856649 40.0 38.0 41.0 32.5 41.0 34-35 37.7191412304788 40.0 38.0 41.0 32.0 41.0 36-37 37.53725674806461 40.0 37.5 41.0 31.5 41.0 38-39 37.13091921369852 40.0 36.0 41.0 31.0 41.0 40-41 37.05720087986392 40.0 35.0 41.0 31.0 41.0 42-43 37.0340015254352 40.0 35.0 41.0 31.0 41.0 44-45 37.00144302814513 40.0 35.0 41.0 31.0 41.0 46-47 37.052401347363585 40.0 35.0 41.0 31.0 41.0 48-49 36.93891410446106 40.0 35.0 41.0 30.5 41.0 50-51 35.534317850922676 38.5 34.0 39.5 28.0 40.0 52-53 35.71038620042037 38.5 35.0 39.5 28.5 40.5 54-55 36.162817693798246 39.0 35.0 41.0 27.5 41.0 56-57 35.83267389184098 39.0 35.0 41.0 25.5 41.0 58-59 35.666261683688 39.0 35.0 41.0 25.5 41.0 60-61 35.44663059589759 39.0 35.0 41.0 25.0 41.0 62-63 35.209066988465395 38.5 35.0 41.0 25.0 41.0 64-65 34.94552905946656 38.0 34.5 40.5 24.5 41.0 66-67 34.66817775053904 37.0 34.0 40.0 24.0 41.0 68-69 34.38797636644592 37.0 34.0 40.0 24.0 41.0 70-71 34.09525540142475 36.0 34.0 39.0 24.0 41.0 72-73 33.75306402921322 36.0 34.0 39.0 24.0 41.0 74-75 33.37517650283699 35.0 33.0 38.5 23.0 40.5 76-77 32.985085700459564 35.0 33.0 37.0 22.0 39.5 78-79 32.60816764950651 35.0 33.0 37.0 20.5 39.0 80-81 32.265030282125736 35.0 33.0 36.0 20.0 39.0 82-83 31.972745161607882 35.0 32.5 36.0 20.0 37.5 84-85 31.691651178334546 35.0 32.0 35.5 19.5 37.0 86-87 31.478218836460073 35.0 32.0 35.0 19.0 37.0 88-89 31.279121374654963 35.0 32.0 35.0 19.0 36.0 90-91 31.099739348620744 35.0 32.0 35.0 18.0 36.0 92-93 30.954041042167987 35.0 31.5 35.0 18.0 36.0 94-95 30.78483139409464 35.0 31.0 35.0 17.0 35.5 96-97 30.653995841651138 35.0 31.0 35.0 17.0 35.0 98-99 30.546521332530318 35.0 31.0 35.0 15.5 35.0 100 30.461578203679153 35.0 31.0 35.0 15.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5138033168892875 1101 2 0.4900799321781477 1101 3 0.49384487949014044 1101 4 0.42257076459378595 1101 5 0.43272460787098765 1101 6 0.4052507174319899 1101 7 0.4351696054323497 1101 8 0.4127541509674728 1101 9 0.4788537036656777 1101 10-11 0.4887817005402155 1101 12-13 0.49395046783535435 1101 14-15 0.5928767609549439 1101 16-17 0.5837498245684287 1101 18-19 0.5805535025824042 1101 20-21 0.6140853630900622 1101 22-23 0.6576796641032061 1101 24-25 0.6462047476008337 1101 26-27 0.6411481574627658 1101 28-29 0.6199672681613961 1101 30-31 0.6060165469191787 1101 32-33 0.5904631540750387 1101 34-35 0.6190020337743789 1101 36-37 0.5891540250581997 1101 38-39 0.5870919220266728 1101 40-41 0.5523755301755031 1101 42-43 0.567800707967308 1101 44-45 0.5864378675709929 1101 46-47 0.6117877859862446 1101 48-49 0.6214541350539449 1101 50-51 0.5371326798539755 1101 52-53 0.6192781231397007 1101 54-55 0.6532734387934767 1101 56-57 0.6468710357798528 1101 58-59 0.5179423576340128 1101 60-61 0.5176725708083936 1101 62-63 0.49280799002816167 1101 64-65 0.469428438291402 1101 66-67 0.4702167261314045 1101 68-69 0.5048038548034981 1101 70-71 0.4466909725725472 1101 72-73 0.4012967798855911 1101 74-75 0.3757171433604043 1101 76-77 0.35861982411596927 1101 78-79 0.3545248478612635 1101 80-81 0.29531981908990446 1101 82-83 0.3329413913341348 1101 84-85 0.3070757393332464 1101 86-87 0.23214623510220278 1101 88-89 0.25324027141746797 1101 90-91 0.23335374807360054 1101 92-93 0.2164689952627299 1101 94-95 0.21641693577757337 1101 96-97 0.19856483100390676 1101 98-99 0.19619421836901907 1101 100 0.21031370954900908 1102 1 0.3964320978153566 1102 2 0.3766003971909271 1102 3 0.39703731727828995 1102 4 0.34096511792824913 1102 5 0.3550682470042119 1102 6 0.3121818521070523 1102 7 0.35537560094434895 1102 8 0.36249320567754495 1102 9 0.4013997964242293 1102 10-11 0.410278891489007 1102 12-13 0.41096784675406894 1102 14-15 0.5033569268251625 1102 16-17 0.4887477221644403 1102 18-19 0.4477641655034432 1102 20-21 0.4743680491117388 1102 22-23 0.4624102510703594 1102 24-25 0.42364635109269244 1102 26-27 0.41907149289240664 1102 28-29 0.4769101128694544 1102 30-31 0.42634099573795226 1102 32-33 0.4125595432301168 1102 34-35 0.40021085246480226 1102 36-37 0.42752002761613284 1102 38-39 0.43969386198382665 1102 40-41 0.40200263134413916 1102 42-43 0.38235786471553723 1102 44-45 0.41728378352646445 1102 46-47 0.4247972368342374 1102 48-49 0.47889779153564405 1102 50-51 0.427948975699195 1102 52-53 0.4870591896344365 1102 54-55 0.5523541900010542 1102 56-57 0.6153635763967316 1102 58-59 0.5053400470085023 1102 60-61 0.48707054043703835 1102 62-63 0.49299266327280833 1102 64-65 0.47424161036583 1102 66-67 0.4346998438062073 1102 68-69 0.412167250012061 1102 70-71 0.4135703204730774 1102 72-73 0.4056633638128062 1102 74-75 0.39610876803811124 1102 76-77 0.40796416433266103 1102 78-79 0.392062247339247 1102 80-81 0.3088876635085711 1102 82-83 0.2831946207976763 1102 84-85 0.28494892002440153 1102 86-87 0.2955433380788719 1102 88-89 0.3167672900408256 1102 90-91 0.2794236989415033 1102 92-93 0.26034761658856453 1102 94-95 0.2665483039761867 1102 96-97 0.24672770257683396 1102 98-99 0.18420614925961232 1102 100 0.16257657011639637 1103 1 0.37071888317782253 1103 2 0.39418699065640084 1103 3 0.40264263599032546 1103 4 0.35188189486046184 1103 5 0.35575674754816333 1103 6 0.34556708835773264 1103 7 0.3247865961041043 1103 8 0.3577079957970426 1103 9 0.39892197067075585 1103 10-11 0.3870454142401485 1103 12-13 0.42091538998229083 1103 14-15 0.49416678386315027 1103 16-17 0.45882531203020704 1103 18-19 0.4388174586011928 1103 20-21 0.5062946672690671 1103 22-23 0.5042542421165095 1103 24-25 0.5085436763569575 1103 26-27 0.46548912604659876 1103 28-29 0.4715485420851522 1103 30-31 0.46959818188369695 1103 32-33 0.41702751407090943 1103 34-35 0.4242591425648641 1103 36-37 0.4507791646693491 1103 38-39 0.4541664016905429 1103 40-41 0.41296026875706815 1103 42-43 0.4356191865772132 1103 44-45 0.44038148269734023 1103 46-47 0.4348652708340879 1103 48-49 0.44084830397805064 1103 50-51 0.3868733580518793 1103 52-53 0.41955504771001983 1103 54-55 0.48560205418185376 1103 56-57 0.5144935109350612 1103 58-59 0.4321555128188521 1103 60-61 0.4185031366126495 1103 62-63 0.4022105913617082 1103 64-65 0.37250420539965745 1103 66-67 0.3917437777458659 1103 68-69 0.37931839203506 1103 70-71 0.35503344563626626 1103 72-73 0.3373829362833902 1103 74-75 0.3141761868494939 1103 76-77 0.3797120902228883 1103 78-79 0.31535445394901984 1103 80-81 0.23414463261729423 1103 82-83 0.2359500870203881 1103 84-85 0.23758398210548037 1103 86-87 0.1862365738183165 1103 88-89 0.15875790524026812 1103 90-91 0.18639011924978988 1103 92-93 0.1509014126889312 1103 94-95 0.1855800474585365 1103 96-97 0.17036021490616093 1103 98-99 0.1288672957475292 1103 100 0.15584484201376014 1104 1 0.27440839851690413 1104 2 0.3014214982533545 1104 3 0.309083318665742 1104 4 0.2498172182670615 1104 5 0.2437601209960647 1104 6 0.2131517235141942 1104 7 0.22959431060387914 1104 8 0.2525405556148641 1104 9 0.2955534201809371 1104 10-11 0.3053396469873917 1104 12-13 0.300556974525243 1104 14-15 0.3601828394554616 1104 16-17 0.3197421502877873 1104 18-19 0.33150159191563944 1104 20-21 0.30293861468913974 1104 22-23 0.3207662996787448 1104 24-25 0.3391047547641861 1104 26-27 0.30252312445065144 1104 28-29 0.2840532792518431 1104 30-31 0.2498761051506051 1104 32-33 0.24782825240669126 1104 34-35 0.23243901131142763 1104 36-37 0.2477699511277578 1104 38-39 0.2338454034908608 1104 40-41 0.24730881183183584 1104 42-43 0.26626641952329066 1104 44-45 0.2473763972790053 1104 46-47 0.26471772720337583 1104 48-49 0.2511071634449493 1104 50-51 0.22114952434654356 1104 52-53 0.26207196000977007 1104 54-55 0.3345865504115295 1104 56-57 0.38450045281398104 1104 58-59 0.3575013176224857 1104 60-61 0.37297738888432264 1104 62-63 0.3241941015342178 1104 64-65 0.29725060653638735 1104 66-67 0.2897555808836998 1104 68-69 0.2922878651546412 1104 70-71 0.2962368697725495 1104 72-73 0.26211528138718876 1104 74-75 0.2742470460793598 1104 76-77 0.28143012745561435 1104 78-79 0.2737080141162451 1104 80-81 0.25582028125611345 1104 82-83 0.2817728680176508 1104 84-85 0.2497211742974521 1104 86-87 0.14411501160783402 1104 88-89 0.1517253556676046 1104 90-91 0.1559637308609929 1104 92-93 0.20136006325262912 1104 94-95 0.24741210191131557 1104 96-97 0.19646313792904735 1104 98-99 0.1853318829193249 1104 100 0.17874596026496548 1105 1 0.2529383617250147 1105 2 0.29448223934511475 1105 3 0.26988249715056156 1105 4 0.21531112223269844 1105 5 0.23250627202144614 1105 6 0.19208016777481873 1105 7 0.21948264357652647 1105 8 0.22686106390838745 1105 9 0.2164439450798099 1105 10-11 0.22376862107467588 1105 12-13 0.22070307120417 1105 14-15 0.29195363474108404 1105 16-17 0.24282773762018195 1105 18-19 0.26774600384607083 1105 20-21 0.29202710400142706 1105 22-23 0.29102766362483123 1105 24-25 0.305867653884647 1105 26-27 0.31033099888485083 1105 28-29 0.31995321174516533 1105 30-31 0.3146511672844383 1105 32-33 0.3275226307235073 1105 34-35 0.33801401350098104 1105 36-37 0.3264347798456768 1105 38-39 0.30300679036631806 1105 40-41 0.30750469409758097 1105 42-43 0.33772237662753923 1105 44-45 0.3401565317588151 1105 46-47 0.33151658452755584 1105 48-49 0.3579334721220775 1105 50-51 0.23823850603617558 1105 52-53 0.29764989157580146 1105 54-55 0.3738529719014281 1105 56-57 0.4181466859971792 1105 58-59 0.39542242039553344 1105 60-61 0.384783166687761 1105 62-63 0.3518239801151637 1105 64-65 0.3620958783407673 1105 66-67 0.31992835277836207 1105 68-69 0.2999107790123716 1105 70-71 0.28830824647697995 1105 72-73 0.316838864138532 1105 74-75 0.30778157539332085 1105 76-77 0.30150097689486444 1105 78-79 0.3423062600588125 1105 80-81 0.2952827723723104 1105 82-83 0.2597358812492878 1105 84-85 0.26813917429633705 1105 86-87 0.20413544302427056 1105 88-89 0.1789915082414133 1105 90-91 0.21505680087518542 1105 92-93 0.187293906440118 1105 94-95 0.20130070227803287 1105 96-97 0.206397999538261 1105 98-99 0.23841293552786524 1105 100 0.22357331981401884 1106 1 0.2368409645481364 1106 2 0.269171086809763 1106 3 0.24711642485898722 1106 4 0.24342154921619397 1106 5 0.24223312308874512 1106 6 0.2136882442464767 1106 7 0.22243882802737858 1106 8 0.22655038210304212 1106 9 0.2233668143988794 1106 10-11 0.23524815687211742 1106 12-13 0.248156829826371 1106 14-15 0.31012302850359674 1106 16-17 0.25490117777783894 1106 18-19 0.23471566372795394 1106 20-21 0.2639472744684568 1106 22-23 0.2571884524427972 1106 24-25 0.25077027553697206 1106 26-27 0.2718692190233085 1106 28-29 0.2549493203770936 1106 30-31 0.2583236145156036 1106 32-33 0.28915499686357293 1106 34-35 0.25990342960747626 1106 36-37 0.22311425036856036 1106 38-39 0.25053059361373187 1106 40-41 0.27199674503684435 1106 42-43 0.273235503718368 1106 44-45 0.30140289929224906 1106 46-47 0.26405469796721803 1106 48-49 0.2906049590640336 1106 50-51 0.20870176712814725 1106 52-53 0.258736995456502 1106 54-55 0.34895841542324746 1106 56-57 0.4005539364945818 1106 58-59 0.3399704254417628 1106 60-61 0.3526935020358195 1106 62-63 0.35300865063554454 1106 64-65 0.377957125963583 1106 66-67 0.3771675526628755 1106 68-69 0.33399241614857544 1106 70-71 0.307453620456279 1106 72-73 0.273829832398782 1106 74-75 0.31766455191330323 1106 76-77 0.3218702488952161 1106 78-79 0.3583299095344188 1106 80-81 0.31538608191809203 1106 82-83 0.33732866213388846 1106 84-85 0.3339927333290156 1106 86-87 0.3178269391297661 1106 88-89 0.28944752009125807 1106 90-91 0.29627420282212213 1106 92-93 0.2714408181374459 1106 94-95 0.30521042881527904 1106 96-97 0.3383812792083738 1106 98-99 0.3070453707083409 1106 100 0.3213188368952409 1107 1 0.1924697646185365 1107 2 0.21647730512765406 1107 3 0.17763023317649385 1107 4 0.18398858722638778 1107 5 0.20405345577250955 1107 6 0.19754285096343693 1107 7 0.20594615687055295 1107 8 0.20079873237902746 1107 9 0.21149059041692198 1107 10-11 0.2063694228583728 1107 12-13 0.19571375518610523 1107 14-15 0.2238038923335921 1107 16-17 0.2205608643175836 1107 18-19 0.1962696243347466 1107 20-21 0.23338295970858525 1107 22-23 0.2423902159196203 1107 24-25 0.2549820426375433 1107 26-27 0.22202000707368796 1107 28-29 0.24587450871408834 1107 30-31 0.23683142805624158 1107 32-33 0.17012393730526298 1107 34-35 0.20097262506529034 1107 36-37 0.18559875153268734 1107 38-39 0.2109208572625576 1107 40-41 0.21907042340225047 1107 42-43 0.2640990653720081 1107 44-45 0.2500120834992927 1107 46-47 0.22584965957941705 1107 48-49 0.2733968575333279 1107 50-51 0.21851951363064614 1107 52-53 0.25417366556319365 1107 54-55 0.3450849272623131 1107 56-57 0.36821006286500335 1107 58-59 0.30774092107331796 1107 60-61 0.340292487289652 1107 62-63 0.3156832837461607 1107 64-65 0.3053959398593378 1107 66-67 0.33603819307513305 1107 68-69 0.31712828663741277 1107 70-71 0.2774273924009236 1107 72-73 0.27590612643825807 1107 74-75 0.3005967527709714 1107 76-77 0.3115270891367672 1107 78-79 0.3197756714050968 1107 80-81 0.2638464482913534 1107 82-83 0.3152035707847354 1107 84-85 0.27373938764737815 1107 86-87 0.2733308480153447 1107 88-89 0.29050029868895066 1107 90-91 0.31176729122973157 1107 92-93 0.26425923959023123 1107 94-95 0.23864708973568582 1107 96-97 0.32853022033177126 1107 98-99 0.30904220337361465 1107 100 0.30283789082381674 1108 1 0.13697117642060164 1108 2 0.1417348847837232 1108 3 0.14526246507190166 1108 4 0.1436547669706414 1108 5 0.13270192469722986 1108 6 0.09292420449985883 1108 7 0.10447601082541524 1108 8 0.14952899537193076 1108 9 0.14736080391084272 1108 10-11 0.1334112887838188 1108 12-13 0.11269873061680613 1108 14-15 0.14796347731787307 1108 16-17 0.13137066514509144 1108 18-19 0.12224519239352105 1108 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-1.1568139897810354 2316 64-65 -1.2350638545966248 2316 66-67 -1.1751957852090698 2316 68-69 -1.099124969917284 2316 70-71 -1.4878225306548032 2316 72-73 -1.142305790430676 2316 74-75 -1.0718265372715905 2316 76-77 -0.989623209323824 2316 78-79 -0.8197010309005819 2316 80-81 -0.8390540484417492 2316 82-83 -0.8153659483926212 2316 84-85 -0.9263734245275153 2316 86-87 -1.0700035076436016 2316 88-89 -0.9916929058672288 2316 90-91 -0.8729468225678154 2316 92-93 -0.790765511427928 2316 94-95 -0.9434661986567505 2316 96-97 -1.0838303741104482 2316 98-99 -0.9755889052987072 2316 100 -0.8946571693577177 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9901.0 3 2517.0 4 1646.0 5 1476.0 6 2973.0 7 18285.0 8 36241.0 9 41414.0 10 42109.0 11 39235.0 12 36909.0 13 37196.0 14 40972.0 15 48177.0 16 60260.0 17 74375.0 18 80601.0 19 89502.0 20 102369.0 21 124212.0 22 161351.0 23 233559.0 24 341749.0 25 425897.0 26 428376.0 27 368757.0 28 329511.0 29 325141.0 30 362391.0 31 438998.0 32 548894.0 33 711711.0 34 982014.0 35 1507968.0 36 2658246.0 37 5108352.0 38 6523743.0 39 1967632.0 40 3646.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 16.231917656283798 32.825756601021574 28.147528007366347 22.794797735328274 2 21.01450375513086 33.941917485226966 26.385292656468874 18.658286103173293 3 25.71106190282218 25.211564686448302 27.275874783176235 21.801498627553283 4 19.319065773699474 27.749829826980505 24.663657850367912 28.267446548952112 5 21.610984370613505 32.37405503048778 26.35398991889652 19.660970680002197 6 28.217804809958842 27.51544616522831 25.409773688918253 18.856975335894596 7 21.11277810302943 25.835355275188217 32.32369008035333 20.728176541429022 8 27.033158946184255 26.818167689557253 25.556248346787186 20.592425017471307 9 26.2094882990566 25.30505643596039 27.381527791117104 21.103927473865905 10-11 20.7097713418051 29.772263501060248 28.97926536056847 20.53869979656618 12-13 20.527570009558872 26.738078379288304 29.045658415566365 23.688693195586456 14-15 20.49144547260037 32.79725435059395 26.135186820273564 20.57611335653211 16-17 23.644425565102633 26.47901656670478 26.20809558728961 23.66846228090298 18-19 20.50644031688788 29.57209777306157 26.25312364946878 23.668338260581766 20-21 23.571863911311695 29.73687975130257 26.179125792231105 20.512130545154626 22-23 23.600535422316998 26.68466115243062 29.166725918626433 20.548077506625948 24-25 20.60711864487689 29.546780772743595 26.045802046933243 23.800298535446267 26-27 20.63791892211307 26.52970580518408 32.26446942320816 20.567905849494693 28-29 20.612209278805626 26.65983401546672 32.23915779946764 20.488798906260012 30-31 20.543835989480684 26.545814133104873 32.288844609134735 20.621505268279712 32-33 20.679318524952723 26.690889954579482 32.03939250189946 20.590399018568338 34-35 20.68905558422813 26.822934613105332 31.925230612454914 20.56277919021162 36-37 20.604698316401198 26.772550112280868 32.02744660364803 20.595304967669907 38-39 20.64131229626702 26.779622465186094 32.02768157346627 20.551383665080625 40-41 20.695185822223703 26.80092735230645 31.995880994876696 20.508005830593156 42-43 20.698765572589995 26.8297197975714 31.94704374171432 20.524470888124284 44-45 20.752821664392165 26.91767030682939 31.885103858396945 20.444404170381503 46-47 20.752661759452465 27.019327106617872 31.81330560515287 20.41470552877679 48-49 20.830479795739894 27.032860181565134 31.681932616987634 20.454727405707338 50-51 20.85488589458604 27.100728482246105 31.607334136802212 20.437051486365643 52-53 21.00836109940473 27.205204986040144 31.357509844334402 20.428924070220724 54-55 21.355352969702487 27.443407589768544 30.633970151212026 20.567269289316943 56-57 21.840672979195684 27.956797938880936 29.535761542211 20.66676753971238 58-59 22.02425402810849 28.44914782727367 28.874082864287104 20.652515280330736 60-61 22.20391582872681 28.680706049659786 28.500910501119776 20.614467620493627 62-63 22.28896229904067 28.859487558801128 28.307751496877593 20.54379864528061 64-65 22.454373082577945 28.926500066224094 28.207974845875672 20.411152005322293 66-67 22.56913897885267 28.759484648741434 28.36824629682918 20.30313007557671 68-69 22.71122169224661 28.621366906790207 28.52498997115525 20.142421429807936 70-71 22.838876882131956 28.463100055797817 28.67118264521681 20.026840416853418 72-73 23.006929661846552 28.353407300680516 28.66896290992497 19.970700127547957 74-75 23.243182150584 28.437866381970128 28.49701329785904 19.82193816958683 76-77 23.56794207730206 28.543445655786336 28.168247444202194 19.72036482270941 78-79 23.848759309334508 28.667301823346936 27.850248453001786 19.63369041431677 80-81 24.146827091087633 28.81058166129203 27.5099718835969 19.53261936402344 82-83 24.346320900426687 28.948807673822618 27.187938419520236 19.516933006230463 84-85 24.50419329878497 28.973865811681236 27.0204010016181 19.501539887915694 86-87 24.699651325375626 28.9724848658138 26.888114275933628 19.439749532876938 88-89 24.95119168126064 28.947697155690093 26.733775158969358 19.36733600407991 90-91 25.166681401552115 28.87493849171654 26.606573721273534 19.35180638545781 92-93 25.309992582939884 28.88959839911877 26.546043706621504 19.254365311319845 94-95 25.468824557540827 28.874735232128064 26.427080022309156 19.229360188021953 96-97 25.588870442389823 28.811097087454172 26.38408670914763 19.215945761008374 98-99 25.738349269733586 28.8005317427428 26.30309233431997 19.158026653203645 100 25.799423232233305 28.82545565769023 26.2169486644747 19.158172445601775 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 28078.0 1 20312.0 2 10809.5 3 8562.0 4 8095.0 5 8441.0 6 8783.0 7 8716.5 8 8552.0 9 8711.0 10 9257.0 11 11842.0 12 102752.0 13 125766.5 14 57845.0 15 59270.5 16 67321.0 17 75068.5 18 82050.0 19 89338.0 20 98600.0 21 111968.5 22 130501.5 23 151898.0 24 174366.5 25 199982.5 26 228435.5 27 262687.0 28 309420.5 29 358174.5 30 396555.0 31 431383.5 32 468514.0 33 500644.5 34 530603.0 35 568765.5 36 608431.0 37 643736.5 38 682062.5 39 711172.5 40 721959.5 41 743838.5 42 780986.5 43 794748.0 44 796706.5 45 812299.0 46 826982.0 47 836977.0 48 836179.0 49 825677.0 50 817663.0 51 809283.5 52 791069.0 53 752461.0 54 700338.5 55 648577.0 56 597212.0 57 540338.0 58 477313.0 59 409007.0 60 335213.0 61 268290.0 62 209675.5 63 156492.0 64 114665.0 65 83645.5 66 61239.0 67 45895.5 68 34500.5 69 26375.0 70 20688.0 71 16391.5 72 13478.5 73 11100.0 74 9204.0 75 7948.0 76 6390.0 77 4493.0 78 3230.0 79 2550.0 80 2087.5 81 1754.5 82 1470.0 83 1227.0 84 1072.5 85 937.5 86 776.5 87 664.0 88 598.0 89 516.0 90 465.0 91 407.0 92 347.5 93 326.5 94 276.0 95 228.0 96 190.0 97 150.0 98 121.0 99 95.5 100 63.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.34895522739125 2 0.32569702840321196 3 0.15004745807541037 4 0.146667288420501 5 0.22000710082355243 6 0.08707432170645439 7 0.11395941806143899 8 0.11711753277551487 9 0.1116237290541537 10-11 0.1074519746564584 12-13 0.10778916919624254 14-15 0.11313904841891535 16-17 0.11671043205065354 18-19 0.12504777265324318 20-21 0.11078485483322728 22-23 0.0918834560269124 24-25 0.0754328858268335 26-27 0.07044076178661458 28-29 0.07934352006262278 30-31 0.08128444473064858 32-33 0.08193621710328014 34-35 0.08232275718547172 36-37 0.07894258753056237 38-39 0.07828259090086291 40-41 0.07909473628631863 42-43 0.0495799337338711 44-45 0.051878613584350815 46-47 0.06659386554310157 48-49 0.0712611314291382 50-51 0.07848614126329358 52-53 0.08251397116230054 54-55 0.08458237181487888 56-57 0.08404162691266406 58-59 0.08322536939867439 60-61 0.08172238641951458 62-63 0.0852362002517774 64-65 0.09051617328937303 66-67 0.09726417621358988 68-69 0.10128378185552893 70-71 0.10011593735188627 72-73 0.09027972589867074 74-75 0.09057579915311535 76-77 0.09299373073107971 78-79 0.0923727993224528 80-81 0.0992030448173487 82-83 0.08700647158564416 84-85 0.09136943996016827 86-87 0.10468245608884107 88-89 0.10936822655328049 90-91 0.1146399753338082 92-93 0.11384222239822132 94-95 0.10706543457426682 96-97 0.10374489078309977 98-99 0.1011727543851122 100 0.0956439975712124 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2.4318306E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.36707731574006 #Duplication Level Percentage of deduplicated Percentage of total 1 71.26000269964939 29.47818041196242 2 14.583274510496402 12.065348883847319 3 5.237184259646112 6.4994101855667274 4 2.548974322828528 4.217744715531356 5 1.5208551336078437 3.1456665948997924 6 0.9559862170004565 2.372781345086384 7 0.6788573032336697 1.9657639784455405 8 0.4764106094793892 1.576617161309819 9 0.38307629641463503 1.4262072094450409 >10 2.0526434061754637 15.492795635323823 >50 0.1508301494675789 4.431350367577434 >100 0.15042880129508684 11.133422346998593 >500 8.493667222659059E-4 0.21507959582266017 >1k 5.871730687684256E-4 0.47221935274161553 >5k 9.93772862733267E-6 0.02966048898808986 >10k+ 2.981318588199793E-5 5.477751726453344 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 1277962 5.255144005507621 No Hit AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 28275 0.11627043429752056 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 26242 0.10791047698799415 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0016407392850472397 4.1121285339529816E-6 0.0 0.0 3.7009156805576835E-5 2 0.0016654120562509577 8.224257067905963E-6 0.0 0.0 3.7009156805576835E-5 3 0.0017229818557262994 8.224257067905963E-6 0.0 0.0 4.52334138734828E-5 4 0.0017723273981337352 1.2336385601858946E-5 0.0 0.0 4.52334138734828E-5 5 0.0017970001693374531 1.6448514135811926E-5 0.0 0.0 5.3457670941388765E-5 6 0.001825785069075124 2.056064266976491E-5 0.0 0.0 5.756979947534175E-5 7 0.001829897197609077 2.056064266976491E-5 0.0 0.0 5.756979947534175E-5 8 0.0018504578402788417 2.056064266976491E-5 0.0 4.1121285339529816E-6 5.756979947534175E-5 9 0.0018792427400165126 2.056064266976491E-5 0.0 4.1121285339529816E-6 6.99061850772007E-5 10-11 0.0019882141461662665 2.26167069367414E-5 0.0 4.1121285339529816E-6 6.99061850772007E-5 12-13 0.0021033537451169504 2.6728835470694382E-5 0.0 4.1121285339529816E-6 7.401831361115367E-5 14-15 0.0021958766371308926 4.317734960650631E-5 0.0 4.1121285339529816E-6 7.401831361115367E-5 16-17 0.0023480253928871526 4.728947814045929E-5 0.0 8.224257067905963E-6 9.457895628091858E-5 18-19 0.0025947531049243314 5.551373520836525E-5 0.0 8.224257067905963E-6 9.869108481487155E-5 20-21 0.0027962474030880273 6.57940565432477E-5 0.0 8.224257067905963E-6 1.110274704167305E-4 22-23 0.002975124994314982 7.401831361115367E-5 0.0 8.224257067905963E-6 1.1925172748463648E-4 24-25 0.0034994213823939875 9.04668277469656E-5 0.0 8.224257067905963E-6 1.315881130864954E-4 26-27 0.005384832315211429 9.457895628091858E-5 0.0 8.224257067905963E-6 1.4598056295533085E-4 28-29 0.010983495314188414 1.0691534188277753E-4 0.0 8.224257067905963E-6 1.5420482002323683E-4 30-31 0.024411651041811876 1.110274704167305E-4 0.0 8.224257067905963E-6 1.5626088429021332E-4 32-33 0.05156814788003737 1.2130779175161297E-4 0.0 8.224257067905963E-6 1.5626088429021332E-4 34-35 0.0991166901181357 1.315881130864954E-4 0.0 1.0280321334882454E-5 1.6242907709114278E-4 36-37 0.17522602108880447 1.398123701544014E-4 0.0 1.2336385601858946E-5 1.7270939842602522E-4 38-39 0.28567779351078154 1.603730128241663E-4 0.0 1.2336385601858946E-5 1.829897197609077E-4 40-41 0.431518544095958 1.7270939842602525E-4 0.0 1.2336385601858946E-5 1.9327004109579015E-4 42-43 0.6156123703682321 1.788775912269547E-4 0.0 1.4392449868835435E-5 2.014942981636961E-4 44-45 0.844065783200524 1.9327004109579013E-4 0.0 1.6448514135811926E-5 2.0560642669764908E-4 46-47 1.1274901302746994 2.0971855523160208E-4 0.0 1.6448514135811926E-5 2.0560642669764908E-4 48-49 1.4680360548140154 2.2616706936741399E-4 0.0 2.26167069367414E-5 2.0971855523160208E-4 50-51 1.8568563122776727 2.3850345496927294E-4 0.0 2.6728835470694382E-5 2.1383068376555506E-4 52-53 2.282844043495464 2.4261558350322591E-4 0.0 3.289702827162385E-5 2.1999887656648452E-4 54-55 2.751715107129584 2.467277120371789E-4 0.0 3.289702827162385E-5 2.241110051004375E-4 56-57 3.277674851200573 2.4878377630415543E-4 0.0 3.289702827162385E-5 2.282231336343905E-4 58-59 3.8615662620578917 2.5495196910508487E-4 0.0 3.289702827162385E-5 2.4055951923624943E-4 60-61 4.491817810006996 2.6523229043996734E-4 0.0 3.289702827162385E-5 2.467277120371789E-4 62-63 5.155433112816329 2.6934441897392026E-4 0.0 3.495309253860035E-5 2.5495196910508487E-4 64-65 5.846003418165723 2.775686760418263E-4 0.0 3.7009156805576835E-5 2.7551261177484975E-4 66-67 6.574703435346196 2.796247403088028E-4 0.0 3.7009156805576835E-5 2.8373686884275575E-4 68-69 7.344574083408606 2.796247403088028E-4 0.0 3.7009156805576835E-5 2.8579293310973227E-4 70-71 8.146408306565434 2.796247403088028E-4 0.0 3.7009156805576835E-5 2.919611259106617E-4 72-73 8.970816059309394 2.8373686884275575E-4 0.0 3.7009156805576835E-5 3.0224144724554417E-4 74-75 9.811328963456582 2.899050616436852E-4 0.0 3.906522107255332E-5 3.166338971143796E-4 76-77 10.673329795257942 2.919611259106617E-4 0.0 4.728947814045929E-5 3.2485815418228556E-4 78-79 11.559830688864594 3.0840964004647366E-4 0.0 4.934554240743578E-5 3.2897028271623854E-4 80-81 12.4652679343701 3.207460256483326E-4 0.0 4.934554240743578E-5 3.330824112501915E-4 82-83 13.38421352210964 3.2897028271623854E-4 0.0 4.934554240743578E-5 3.35138475517168E-4 84-85 14.314389332875407 3.35138475517168E-4 0.0 5.140160667441228E-5 3.4130666831809747E-4 86-87 15.252838746251486 3.577551824539094E-4 0.0 5.3457670941388765E-5 3.4541879685205044E-4 88 15.962250824543453 3.7009156805576835E-4 0.0 5.3457670941388765E-5 3.4953092538600347E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 163175 0.0 85.738205 8 AGTGGTA 164095 0.0 85.23434 5 TGGTATC 164735 0.0 84.58745 7 GTATCAA 165540 0.0 84.4218 9 GTGGTAT 166545 0.0 84.01483 6 GCAGTGG 171595 0.0 82.046104 3 AAGCAGT 172320 0.0 81.67835 1 CAGTGGT 174945 0.0 80.03456 4 AGCAGTG 179790 0.0 78.3822 2 CAACGCA 160585 0.0 44.819286 12-13 ATCAACG 161450 0.0 44.64858 10-11 ACGCAGA 162735 0.0 44.249523 14-15 AGAGTAC 162670 0.0 43.710773 18-19 TCAACGC 160630 0.0 43.62152 12-13 AACGCAG 162140 0.0 43.31882 14-15 GAGTACT 159100 0.0 43.15985 20-21 CGCAGAG 163920 0.0 42.788383 16-17 TATCAAC 164945 0.0 42.304806 10-11 AGTACTT 167815 0.0 42.04742 20-21 GTACTTT 168210 0.0 40.820442 22-23 >>END_MODULE