FastQCFastQC Report
Thu 26 May 2016
SRR1660728_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660728_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24318306
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT12145084.994213001514168No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT303750.12490590421882182No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1605050.084.924248
AGTGGTA1612100.084.226995
TGGTATC1627500.083.726087
GTATCAA1629250.083.5646069
GTGGTAT1633000.083.114546
GCAGTGG1643650.082.743393
AAGCAGT1620050.082.2063451
CAGTGGT1711200.079.2148744
AGCAGTG1732150.078.374792
CAACGCA1574350.044.5291112-13
ATCAACG1586200.044.2630610-11
ACGCAGA1604250.043.84977314-15
AGAGTAC1622400.043.25126318-19
TCAACGC1573900.043.19795612-13
AACGCAG1590600.042.86387314-15
GAGTACT1583850.042.72450320-21
CGCAGAG1613750.042.27384616-17
TATCAAC1621900.041.91526410-11
AGTACTT1670050.041.75214820-21
GTACTTT1672750.040.43100422-23
Produced by FastQC (version 0.11.5)