##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660728_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 24318306 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.497417583280676 34.0 31.0 34.0 28.0 34.0 2 32.065486962784334 34.0 31.0 34.0 30.0 34.0 3 31.900921593798515 34.0 31.0 34.0 30.0 34.0 4 35.718244354684906 37.0 35.0 37.0 35.0 37.0 5 35.96439928833859 37.0 35.0 37.0 35.0 37.0 6 35.54405594698907 37.0 35.0 37.0 35.0 37.0 7 35.692828110642246 37.0 35.0 37.0 35.0 37.0 8 35.990605225544904 37.0 35.0 37.0 35.0 37.0 9 37.79495269941911 39.0 38.0 39.0 35.0 39.0 10-11 37.78770682053265 39.0 37.5 39.0 35.0 39.0 12-13 37.863184939773355 39.0 38.0 39.0 35.0 39.0 14-15 39.31866463066959 41.0 39.0 41.0 36.0 41.0 16-17 39.29045797844636 41.0 39.0 41.0 36.0 41.0 18-19 39.29302869616001 41.0 39.0 41.0 36.0 41.0 20-21 39.23710062288056 40.5 39.0 41.0 36.0 41.0 22-23 39.115087025387375 40.0 39.0 41.0 35.5 41.0 24-25 39.12626196084546 40.0 39.0 41.0 36.0 41.0 26-27 39.07797537377809 40.0 39.0 41.0 35.5 41.0 28-29 39.00620976642041 40.0 39.0 41.0 35.0 41.0 30-31 38.9160206101527 40.0 38.5 41.0 35.0 41.0 32-33 38.799045809358596 40.0 38.0 41.0 35.0 41.0 34-35 38.708034391869234 40.0 38.0 41.0 35.0 41.0 36-37 38.578314644942786 40.0 38.0 41.0 35.0 41.0 38-39 37.98214332856902 40.0 37.0 41.0 33.5 41.0 40-41 37.87051010049795 40.0 37.0 41.0 33.0 41.0 42-43 37.89182015803239 40.0 37.0 41.0 33.0 41.0 44-45 37.79729028000553 40.0 36.5 41.0 33.0 41.0 46-47 37.65481434027518 40.0 36.0 41.0 33.0 41.0 48-49 37.65326178558655 40.0 36.0 41.0 33.0 41.0 50-51 37.61127923548622 40.0 36.0 41.0 33.0 41.0 52-53 37.35490416149875 40.0 36.0 41.0 32.5 41.0 54-55 36.85304221025922 40.0 35.5 41.0 30.5 41.0 56-57 36.39414215365166 39.0 35.0 41.0 29.5 41.0 58-59 36.26142805753 39.0 35.0 41.0 29.0 41.0 60-61 36.124972952474565 39.0 35.0 41.0 29.0 41.0 62-63 35.90114356649678 39.0 35.0 41.0 28.5 41.0 64-65 35.64290249082317 38.0 35.0 40.5 28.0 41.0 66-67 35.36948410386809 37.5 35.0 40.0 28.0 41.0 68-69 35.107231173914826 37.0 35.0 40.0 28.0 41.0 70-71 34.647646283421224 36.5 34.5 39.0 27.0 41.0 72-73 34.28185302051878 36.0 34.0 39.0 26.0 41.0 74-75 34.05464258077845 35.0 34.0 38.5 26.5 40.0 76-77 32.38494143054208 34.5 32.0 36.5 24.5 39.0 78-79 33.30621762881016 35.0 33.5 37.0 26.0 39.0 80-81 33.2018249955404 35.0 34.0 36.5 26.0 39.0 82-83 32.94571671645221 35.0 34.0 36.0 26.0 37.5 84-85 32.7021914478747 35.0 34.0 36.0 25.5 37.0 86-87 32.489373663609626 35.0 34.0 35.0 25.0 37.0 88-89 32.314646793242915 35.0 33.0 35.0 25.0 36.0 90-91 32.153413605372016 35.0 33.0 35.0 25.0 36.0 92-93 32.008575330041495 35.0 33.0 35.0 24.0 36.0 94-95 31.89571356244962 35.0 33.0 35.0 24.0 36.0 96-97 31.784690594813636 35.0 33.0 35.0 23.5 35.0 98-99 31.689000993736983 35.0 33.0 35.0 23.0 35.0 100 31.628582805068742 35.0 33.0 35.0 23.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.2813710238707117 1101 2 0.9579684038562917 1101 3 1.2496530002137796 1101 4 0.7683978082500502 1101 5 0.4748531543928678 1101 6 0.9147399020941975 1101 7 0.6972406256915846 1101 8 0.4159631528729193 1101 9 0.46726701099780854 1101 10-11 0.39676660257809715 1101 12-13 0.36715944136861367 1101 14-15 0.4548215181708386 1101 16-17 0.47954764598038935 1101 18-19 0.46268492305921427 1101 20-21 0.45775854933328475 1101 22-23 0.4322543694421128 1101 24-25 0.43854815092108623 1101 26-27 0.4646272070606656 1101 28-29 0.4340294600636909 1101 30-31 0.4433010761652696 1101 32-33 0.4669465612136321 1101 34-35 0.503361127363263 1101 36-37 0.48359481112228764 1101 38-39 0.7067327199290432 1101 40-41 0.6891398632161483 1101 42-43 0.5285897398733752 1101 44-45 0.45303832217745565 1101 46-47 0.4721027028178213 1101 48-49 0.4473299893729177 1101 50-51 0.43240860305275675 1101 52-53 0.42294848719486566 1101 54-55 0.4574983379463191 1101 56-57 0.456718477098498 1101 58-59 0.5079369627947443 1101 60-61 0.5360890058484173 1101 62-63 0.4910583066334766 1101 64-65 0.46544914445570384 1101 66-67 0.47574921797461656 1101 68-69 0.41895985220430987 1101 70-71 0.5273879819163696 1101 72-73 0.5561798613889621 1101 74-75 0.427286542416347 1101 76-77 0.40019432302388935 1101 78-79 0.4403323128018499 1101 80-81 0.4364585693426619 1101 82-83 0.4108353668505984 1101 84-85 0.4021133639605523 1101 86-87 0.384659373450404 1101 88-89 0.3799470217194525 1101 90-91 0.34627299681427104 1101 92-93 0.32246950609161473 1101 94-95 0.28583201226117794 1101 96-97 0.2745576800415641 1101 98-99 0.2505916432304076 1101 100 0.21580886618345474 1102 1 1.2110097920061094 1102 2 0.9162472067473857 1102 3 1.1950812691545032 1102 4 0.7306567104578576 1102 5 0.4127351858177235 1102 6 0.8580099800896406 1102 7 0.6475644028762417 1102 8 0.38080789623188593 1102 9 0.3929464511845495 1102 10-11 0.30872120698223426 1102 12-13 0.32832402058000554 1102 14-15 0.43039035405887915 1102 16-17 0.44519183558225706 1102 18-19 0.3690441816936456 1102 20-21 0.36251941335746807 1102 22-23 0.4038912916541122 1102 24-25 0.38059735964622377 1102 26-27 0.3929546518953728 1102 28-29 0.4241382647911891 1102 30-31 0.40719169952811995 1102 32-33 0.3969881791289538 1102 34-35 0.38923679250321896 1102 36-37 0.33193407751411286 1102 38-39 0.6032648138210988 1102 40-41 0.599345138302219 1102 42-43 0.44232264995275017 1102 44-45 0.38748533715337885 1102 46-47 0.39756681929620896 1102 48-49 0.3780102571532922 1102 50-51 0.3421500298909024 1102 52-53 0.3880628276761726 1102 54-55 0.42539532480596876 1102 56-57 0.4459176607020723 1102 58-59 0.4496786586848387 1102 60-61 0.462841391500028 1102 62-63 0.43129339998426985 1102 64-65 0.42519602676521373 1102 66-67 0.4014557998471062 1102 68-69 0.3796713335456374 1102 70-71 0.5416331586411545 1102 72-73 0.5335020145782465 1102 74-75 0.38092511155088715 1102 76-77 0.18196319694465046 1102 78-79 0.4035468224089911 1102 80-81 0.41109523656769653 1102 82-83 0.41736547894903 1102 84-85 0.41386657272492045 1102 86-87 0.3763200351155618 1102 88-89 0.359400002613647 1102 90-91 0.34507946713525683 1102 92-93 0.33289284444627043 1102 94-95 0.2732528615616836 1102 96-97 0.25673990144826675 1102 98-99 0.2111440844499164 1102 100 0.2220271252167052 1103 1 1.190666320249015 1103 2 0.894888477657922 1103 3 1.1494288791686778 1103 4 0.7274069305991091 1103 5 0.424666942866331 1103 6 0.8327246714220635 1103 7 0.6411786980727996 1103 8 0.3840476039099414 1103 9 0.39799870091064093 1103 10-11 0.3372917214009661 1103 12-13 0.30003880760969537 1103 14-15 0.40002620603224415 1103 16-17 0.40768392409800924 1103 18-19 0.34725926190810696 1103 20-21 0.3569909470011865 1103 22-23 0.36594820305570863 1103 24-25 0.35926926292280115 1103 26-27 0.36160385753586155 1103 28-29 0.35366592514201756 1103 30-31 0.34770684998795787 1103 32-33 0.3621616276824824 1103 34-35 0.37635912254513215 1103 36-37 0.34530389247071014 1103 38-39 0.6166176839507926 1103 40-41 0.5690764103268151 1103 42-43 0.42927496574287005 1103 44-45 0.37329220805545305 1103 46-47 0.3799546295550371 1103 48-49 0.3650549147421742 1103 50-51 0.37509497785774926 1103 52-53 0.3637902120931358 1103 54-55 0.39095662162002043 1103 56-57 0.45372753951306777 1103 58-59 0.4396954238988968 1103 60-61 0.43607254417737096 1103 62-63 0.3687547796108248 1103 64-65 0.33799690212088507 1103 66-67 0.3798028348691318 1103 68-69 0.38348890371405275 1103 70-71 0.5204996848632746 1103 72-73 0.5615722003516481 1103 74-75 0.4136324161169469 1103 76-77 0.2963750898799091 1103 78-79 0.4388400611862622 1103 80-81 0.4680320842835428 1103 82-83 0.466958384391404 1103 84-85 0.42428910243742024 1103 86-87 0.3862958023959777 1103 88-89 0.3564214035142186 1103 90-91 0.3638605496163052 1103 92-93 0.3741178872833615 1103 94-95 0.36802081994509805 1103 96-97 0.36600038796578005 1103 98-99 0.32610878070580895 1103 100 0.3404202843579114 1104 1 1.1032498504339046 1104 2 0.8439761838727691 1104 3 1.1319910267435738 1104 4 0.7180518175450672 1104 5 0.39904875117762373 1104 6 0.808538491459359 1104 7 0.6341447528807294 1104 8 0.3455543558348637 1104 9 0.36853438605457 1104 10-11 0.28412292876374323 1104 12-13 0.2627090515370938 1104 14-15 0.2932593148446898 1104 16-17 0.31300092709815175 1104 18-19 0.29074484241404264 1104 20-21 0.3062629971213795 1104 22-23 0.29651113272977625 1104 24-25 0.2834368622416932 1104 26-27 0.292041948310235 1104 28-29 0.2512035331287308 1104 30-31 0.279521060798686 1104 32-33 0.2921027876671687 1104 34-35 0.3295147184006453 1104 36-37 0.31949571810690713 1104 38-39 0.5487190110605837 1104 40-41 0.48917425917917257 1104 42-43 0.3466657813329732 1104 44-45 0.30255838743993735 1104 46-47 0.34051880519647426 1104 48-49 0.2884413389318965 1104 50-51 0.2745793825171532 1104 52-53 0.30926294965042445 1104 54-55 0.354430482109251 1104 56-57 0.3773920042272252 1104 58-59 0.3524897765217716 1104 60-61 0.3535926701722758 1104 62-63 0.29814183089462176 1104 64-65 0.2653036828355013 1104 66-67 0.2956766928815071 1104 68-69 0.26941901655328593 1104 70-71 0.43164016582070275 1104 72-73 0.44048482228792096 1104 74-75 0.3190866497151461 1104 76-77 0.20289264526919482 1104 78-79 0.2898494354896073 1104 80-81 0.3417984849756692 1104 82-83 0.3361380595887056 1104 84-85 0.2983439003395745 1104 86-87 0.305764351161983 1104 88-89 0.2618143976725946 1104 90-91 0.26660689176315344 1104 92-93 0.32062978457239666 1104 94-95 0.2905622634954277 1104 96-97 0.2704199598902228 1104 98-99 0.25484268741638516 1104 100 0.2947295189729289 1105 1 1.0760879392144602 1105 2 0.7965683439546041 1105 3 1.0697444769012243 1105 4 0.6606992981186934 1105 5 0.35196742081013355 1105 6 0.7968305081743097 1105 7 0.5804303397596868 1105 8 0.2950034049314141 1105 9 0.3115634884019798 1105 10-11 0.21526010193762346 1105 12-13 0.21403165164755222 1105 14-15 0.2899242494667078 1105 16-17 0.28541545498932663 1105 18-19 0.2493044211935853 1105 20-21 0.26666813876816065 1105 22-23 0.24692531261072048 1105 24-25 0.22921331158678981 1105 26-27 0.25476547562632845 1105 28-29 0.2506849225312422 1105 30-31 0.2691783788456732 1105 32-33 0.2901614675847739 1105 34-35 0.29495474679415423 1105 36-37 0.23420248074014438 1105 38-39 0.5002608372851967 1105 40-41 0.4928262322263848 1105 42-43 0.31218820655283963 1105 44-45 0.2454928037418327 1105 46-47 0.2737577360816559 1105 48-49 0.2523340612902061 1105 50-51 0.2402223395899341 1105 52-53 0.22469322049111895 1105 54-55 0.27411613835045046 1105 56-57 0.24276311291192343 1105 58-59 0.20466504035139366 1105 60-61 0.21351599436290059 1105 62-63 0.20370647672672249 1105 64-65 0.19432996709709727 1105 66-67 0.23902075279467994 1105 68-69 0.23554301599537553 1105 70-71 0.37287385704737375 1105 72-73 0.36966093751805573 1105 74-75 0.22954295472460018 1105 76-77 0.13571896775163594 1105 78-79 0.2745867475045216 1105 80-81 0.27326030281468405 1105 82-83 0.2856967217423332 1105 84-85 0.261685471730452 1105 86-87 0.2561155549879075 1105 88-89 0.2791180284804753 1105 90-91 0.26976180543263695 1105 92-93 0.22493817235845626 1105 94-95 0.22031151393877835 1105 96-97 0.2119867204668573 1105 98-99 0.20806757184892177 1105 100 0.18851878007771106 1106 1 1.071755654039947 1106 2 0.7933454124991712 1106 3 1.0492520305487538 1106 4 0.6207625642317822 1106 5 0.3388105170838358 1106 6 0.7843593011598031 1106 7 0.5525756760805862 1106 8 0.2779676859383997 1106 9 0.25752054527244184 1106 10-11 0.1918036256341722 1106 12-13 0.18362327767452769 1106 14-15 0.22926205787332066 1106 16-17 0.2284375102593117 1106 18-19 0.19675918403981285 1106 20-21 0.20838784101574248 1106 22-23 0.2326049212144099 1106 24-25 0.23442507732643492 1106 26-27 0.21025246151070576 1106 28-29 0.22365639001635174 1106 30-31 0.23991152514228276 1106 32-33 0.23407555622009824 1106 34-35 0.254387234696388 1106 36-37 0.24578636168939028 1106 38-39 0.5090831021869349 1106 40-41 0.4982961149204712 1106 42-43 0.3345259145370463 1106 44-45 0.275355915455485 1106 46-47 0.28915845284655006 1106 48-49 0.2677692982556934 1106 50-51 0.2669331293493542 1106 52-53 0.27022771872430695 1106 54-55 0.272251262968382 1106 56-57 0.3006260300571668 1106 58-59 0.29441050094501975 1106 60-61 0.31384503463855395 1106 62-63 0.2925897168555167 1106 64-65 0.25146952912989917 1106 66-67 0.21509825378321068 1106 68-69 0.220154067480685 1106 70-71 0.38238966416104603 1106 72-73 0.33109402795470544 1106 74-75 0.24144536297932717 1106 76-77 0.11647552502064684 1106 78-79 0.24168428903859507 1106 80-81 0.23501584330687564 1106 82-83 0.2349802706700146 1106 84-85 0.22574523561227977 1106 86-87 0.22925334707588263 1106 88-89 0.2366181696994829 1106 90-91 0.2289000870798148 1106 92-93 0.2390467352511294 1106 94-95 0.25820371049052326 1106 96-97 0.2679871158800644 1106 98-99 0.2646618716223408 1106 100 0.2874191547787994 1107 1 1.0182923758557578 1107 2 0.7168852704646866 1107 3 1.0040107321977665 1107 4 0.615028967977274 1107 5 0.31673015448662056 1107 6 0.7390383238646763 1107 7 0.5261895682592126 1107 8 0.2501949686760838 1107 9 0.2271247928177118 1107 10-11 0.16256787144855167 1107 12-13 0.12264466866679413 1107 14-15 0.17258107147893043 1107 16-17 0.15843730356787233 1107 18-19 0.10885518387155457 1107 20-21 0.12221097140709247 1107 22-23 0.15968221849301756 1107 24-25 0.14221670987692647 1107 26-27 0.12766513317036043 1107 28-29 0.10324649914218043 1107 30-31 0.11919173611743616 1107 32-33 0.1447015991959617 1107 34-35 0.13553330123749419 1107 36-37 0.14009616459279073 1107 38-39 0.36837707929520036 1107 40-41 0.3434555102791208 1107 42-43 0.2197280164559956 1107 44-45 0.16219982425887025 1107 46-47 0.20493187406847113 1107 48-49 0.16947297961147 1107 50-51 0.13469162237297638 1107 52-53 0.1323666281816216 1107 54-55 0.17749403958618615 1107 56-57 0.18474802417745906 1107 58-59 0.17768906104786453 1107 60-61 0.1891298215928643 1107 62-63 0.16603337264924534 1107 64-65 0.13671883768056858 1107 66-67 0.15213619870466033 1107 68-69 0.12273583086565054 1107 70-71 0.29642198804241815 1107 72-73 0.3446597670724003 1107 74-75 0.19440354652180503 1107 76-77 0.02581899324491843 1107 78-79 0.17137312052125253 1107 80-81 0.21463994169103984 1107 82-83 0.20547788453222182 1107 84-85 0.21295733317739973 1107 86-87 0.19127187992446437 1107 88-89 0.18800548624270874 1107 90-91 0.21089601199498276 1107 92-93 0.18562146532540424 1107 94-95 0.16311248704402814 1107 96-97 0.18163507830138315 1107 98-99 0.17804815180745237 1107 100 0.18285028289582073 1108 1 1.0039851752429243 1108 2 0.7041809182641501 1108 3 0.9808858385334069 1108 4 0.5831060704799 1108 5 0.26841099257683965 1108 6 0.6691660226946752 1108 7 0.4813771406230529 1108 8 0.21054768581080907 1108 9 0.16055792074663344 1108 10-11 0.10352948459640743 1108 12-13 0.10526189639774941 1108 14-15 0.11490760956051105 1108 16-17 0.11041133549367288 1108 18-19 0.07982967914514916 1108 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-0.7177426992324385 2316 76-77 -0.617567653325402 2316 78-79 -0.8448878449447719 2316 80-81 -1.0279513552361905 2316 82-83 -1.0932590214993603 2316 84-85 -0.8678454085441665 2316 86-87 -0.7388860645015107 2316 88-89 -0.6477687806430446 2316 90-91 -0.6450416168250115 2316 92-93 -0.7179621093647874 2316 94-95 -0.7332694287246433 2316 96-97 -0.8188428281675115 2316 98-99 -0.9328629339681456 2316 100 -0.8475425440340238 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 30.0 7 645.0 8 843.0 9 351.0 10 266.0 11 498.0 12 691.0 13 1091.0 14 2605.0 15 6528.0 16 14333.0 17 22491.0 18 28476.0 19 36307.0 20 48345.0 21 68983.0 22 103900.0 23 171696.0 24 288328.0 25 396478.0 26 408395.0 27 346700.0 28 303892.0 29 313991.0 30 360456.0 31 438324.0 32 540677.0 33 690003.0 34 947093.0 35 1470061.0 36 2686963.0 37 5385374.0 38 7152252.0 39 2077235.0 40 4005.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 16.445232209331728 32.30623280335862 28.482333669892206 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26.325692669546967 23.607201504907454 20-21 23.46486895444311 29.76548900544474 26.272662437372375 20.496979602739778 22-23 23.464089900315113 26.764579560735623 29.248825965775932 20.522504573173332 24-25 20.559336660235754 29.56903315668729 26.133579738464785 23.73805044461217 26-27 20.60958643235534 26.58724838921312 32.25363808247995 20.549527095951586 28-29 20.579670404347485 26.708085070404486 32.22785675068796 20.484387774560066 30-31 20.513440628344785 26.62211730801081 32.24946228953574 20.614979774108658 32-33 20.676865410352104 26.778201147212155 31.986836479897978 20.558096962537764 34-35 20.69691790870672 26.888898630836444 31.874348972764405 20.53983448769243 36-37 20.60587995160155 26.84240266881504 31.953990057041263 20.597727322542145 38-39 20.63474671862903 26.824970298728722 32.002769195204145 20.5375137874381 40-41 20.66168689538111 26.865800629916563 31.977739589592957 20.494772885109366 42-43 20.660172517001545 26.90115097016394 31.920578514113167 20.51809799872135 44-45 20.712222713637868 26.988109398103127 31.845128182874628 20.45453970538438 46-47 20.740519816978054 27.09779966376354 31.713342889908493 20.448337629349915 48-49 20.789546913778555 27.117904594743514 31.61366294656492 20.478885544913012 50-51 20.815677046658667 27.22169335975534 31.4914460438112 20.47118354977479 52-53 20.93318922468298 27.338045938540844 31.220068020983526 20.50869681579265 54-55 21.212021992159386 27.578486984780376 30.528160147121163 20.68133087593908 56-57 21.642695395598828 28.101061921011794 29.424645689567114 20.83159699382227 58-59 21.74663754017189 28.645888418927974 28.699350514811993 20.908123526088144 60-61 21.776482259925245 28.921214043597416 28.31423584255304 20.988067853924296 62-63 21.74430681760412 29.12725184187203 28.098329338449545 21.030112002074304 64-65 21.7868918052016 29.22516965493796 27.965185825411254 21.022752714449183 66-67 21.71104001283471 29.115215202552957 28.10532692009054 21.068417864521788 68-69 21.644779860449628 29.002473797024408 28.265468636132983 21.087277706392975 70-71 21.68378459830097 28.894067596632183 28.319221702493675 21.10292610257317 72-73 21.49149768607196 28.84919062816457 28.370276788837884 21.289034896925585 74-75 21.529417173720994 28.92674606532117 28.204524203094916 21.339312557862925 76-77 21.56270705978292 29.01371487269901 28.00364422132191 21.41993384619616 78-79 21.617548216181387 29.185416083913353 27.656771938777545 21.54026376112772 80-81 21.651966312127495 29.39696387863907 27.30658498264057 21.644484826592866 82-83 21.61081578342663 29.55494159431613 26.97817420658887 21.856068415668368 84-85 21.567943794312967 29.585959093728796 26.81113571101402 22.03496140094421 86-87 21.576543302727412 29.606491706251614 26.660405678772115 22.156559312248863 88-89 21.56996282459972 29.627569811541687 26.516134475082538 22.286332888776055 90-91 21.590397941981223 29.570513442237374 26.39796625419416 22.441122361587244 92-93 21.564011894206313 29.574397920655333 26.352698957149457 22.508891227988904 94-95 21.568915784933935 29.53842781899036 26.248765357479975 22.64389103859573 96-97 21.586381618332297 29.41654580502085 26.209044359824148 22.788028216822706 98-99 21.59577700138278 29.329933244030286 26.200264453675594 22.874025300911338 100 21.550499504213363 29.275653915266876 26.159505779352248 23.014340801167517 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 18864.0 1 14753.0 2 8829.0 3 6617.0 4 6036.5 5 6214.5 6 6601.5 7 6572.0 8 6498.5 9 6816.0 10 7599.5 11 13311.5 12 105666.5 13 124918.0 14 54437.0 15 55377.5 16 63582.5 17 71572.5 18 79006.0 19 87091.0 20 97061.0 21 111333.5 22 130985.5 23 153987.0 24 178025.0 25 204416.0 26 233782.5 27 270398.5 28 318660.5 29 368817.0 30 409038.5 31 444556.5 32 481267.5 33 513537.0 34 543049.5 35 578674.0 36 616652.5 37 650810.0 38 687041.5 39 715135.0 40 728665.5 41 752655.0 42 791132.0 43 806869.0 44 808684.0 45 821837.5 46 832079.5 47 836671.5 48 832319.5 49 822276.0 50 818155.0 51 811916.5 52 794354.0 53 756370.0 54 701331.0 55 646701.5 56 592855.5 57 533061.5 58 467348.0 59 395907.0 60 321885.5 61 255973.0 62 198905.5 63 147528.0 64 106481.0 65 76321.0 66 54299.5 67 39756.5 68 29271.0 69 21646.5 70 16405.0 71 12501.0 72 9778.0 73 7610.5 74 6102.0 75 5206.0 76 3851.0 77 2145.5 78 1177.5 79 753.0 80 459.5 81 306.5 82 204.0 83 120.5 84 79.0 85 58.0 86 39.5 87 27.5 88 16.5 89 11.5 90 9.5 91 7.5 92 7.0 93 5.0 94 3.0 95 1.0 96 1.5 97 1.0 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.29578129331870406 2 0.004922217855141719 3 1.8919368808008257 4 6.044828944910883E-4 5 2.8784899737670873E-5 6 0.00743472838938699 7 0.005789876975805798 8 2.467277120371789E-4 9 0.0 10-11 4.52334138734828E-5 12-13 3.289702827162385E-5 14-15 0.0 16-17 2.8784899737670873E-5 18-19 0.0 20-21 2.6523229043996734E-4 22-23 5.201842595450521E-4 24-25 3.6803550378879183E-4 26-27 0.0028291444313596516 28-29 0.004839975284462659 30-31 0.004455491266538056 32-33 0.012030032026079449 34-35 0.007924071684927396 36-37 0.004099792148351123 38-39 0.6001240382450981 40-41 0.006552676818854077 42-43 0.014807774850764689 44-45 0.02655818213653533 46-47 0.02569669120867218 48-49 0.031215167701237084 50-51 0.03399907871872325 52-53 0.051693567800322936 54-55 0.030676478863289246 56-57 0.02428006292872538 58-59 0.019814291340852445 60-61 0.020877276566879287 62-63 0.022789416335167425 64-65 0.03776784452009116 66-67 0.037959058496919974 68-69 0.03915157577176634 70-71 0.14792148762335666 72-73 0.05386477166625011 74-75 0.05131319591093228 76-77 0.05336103592084087 78-79 0.04932498176476602 80-81 0.0478487276210769 82-83 0.0457494860044939 84-85 0.04646499636940172 86-87 0.05251188137857958 88-89 0.05284701985409675 90-91 0.04701602159295142 92-93 0.05011245437901801 94-95 0.05633616091515585 96-97 0.05615522725966192 98-99 0.03678093367194245 100 0.005732307176330457 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2.4318306E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.47258529564907 #Duplication Level Percentage of deduplicated Percentage of total 1 69.52800305477848 28.139780340706714 2 15.521603588450047 12.563988503175946 3 5.539919112696468 6.72644546458813 4 2.72855510306361 4.41726716570482 5 1.5610287608059092 3.158943483533927 6 1.0391099570131708 2.5233279820064283 7 0.6919477823350441 1.9603440948483204 8 0.5171395277984071 1.6743978918858173 9 0.4014142051887875 1.4621643592549545 >10 2.1525098647975818 15.803871672580339 >50 0.16183313691533557 4.6424395807158465 >100 0.15557254790015743 10.985792203876963 >500 7.401768560309964E-4 0.1849851581674143 >1k 5.721877105107101E-4 0.4183071491655573 >5k 1.0198738117898187E-5 0.03187817291424435 >10k+ 4.079495247159274E-5 5.306066776874636 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 1214508 4.994213001514168 No Hit AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 30375 0.12490590421882182 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0016489635421151458 8.224257067905963E-6 0.0 0.0 4.52334138734828E-5 2 0.0016736363133188635 1.6448514135811926E-5 0.0 0.0 4.52334138734828E-5 3 0.0017435424983960643 1.6448514135811926E-5 0.0 0.0 5.3457670941388765E-5 4 0.0017928880408035 2.467277120371789E-5 0.0 4.1121285339529816E-6 5.3457670941388765E-5 5 0.001805224426405359 4.112128533952982E-5 0.0 4.1121285339529816E-6 5.3457670941388765E-5 6 0.001825785069075124 4.112128533952982E-5 0.0 4.1121285339529816E-6 5.3457670941388765E-5 7 0.0018586820973467478 4.112128533952982E-5 0.0 4.1121285339529816E-6 5.3457670941388765E-5 8 0.0018833548685504656 4.112128533952982E-5 0.0 4.1121285339529816E-6 6.168192800929473E-5 9 0.0019039155112202306 4.112128533952982E-5 0.0 4.1121285339529816E-6 6.57940565432477E-5 10-11 0.00199232627470022 4.52334138734828E-5 0.0 4.1121285339529816E-6 6.57940565432477E-5 12-13 0.0021177461949857855 4.52334138734828E-5 0.0 4.1121285339529816E-6 6.99061850772007E-5 14-15 0.0022493343080722808 4.728947814045929E-5 0.0 4.1121285339529816E-6 6.99061850772007E-5 16-17 0.0024055951923624944 5.756979947534175E-5 0.0 4.1121285339529816E-6 6.99061850772007E-5 18-19 0.0026543789686666497 6.168192800929473E-5 0.0 4.1121285339529816E-6 7.401831361115367E-5 20-21 0.002866153588165228 7.196224934417718E-5 0.0 4.1121285339529816E-6 7.813044214510666E-5 22-23 0.003061479693527995 7.401831361115367E-5 0.0 4.1121285339529816E-6 8.429863494603612E-5 24-25 0.00362689736694653 8.018650641208315E-5 0.0 4.1121285339529816E-6 8.635469921301261E-5 26-27 0.005549317456569549 8.635469921301261E-5 0.0 4.1121285339529816E-6 9.04668277469656E-5 28-29 0.011180877483818158 9.04668277469656E-5 0.0 4.1121285339529816E-6 9.663502054789506E-5 30-31 0.024580248311703946 1.0074714908184805E-4 0.0 1.0280321334882454E-5 1.0691534188277753E-4 32-33 0.05157842820137225 1.1925172748463648E-4 0.0 1.2336385601858946E-5 1.0897140614975402E-4 34-35 0.0994148194368473 1.2336385601858946E-4 0.0 1.2336385601858946E-5 1.151395989506835E-4 36-37 0.17537405771602677 1.315881130864954E-4 0.0 1.2336385601858946E-5 1.151395989506835E-4 38-39 0.2857086344747862 1.3775630588742487E-4 0.0 2.467277120371789E-5 1.151395989506835E-4 40-41 0.43263498699292624 1.4186843442137788E-4 0.0 2.467277120371789E-5 1.1719566321765998E-4 42-43 0.6181022641955406 1.4803662722230734E-4 0.0 2.467277120371789E-5 1.2130779175161297E-4 44-45 0.848173799605943 1.5420482002323683E-4 0.0 2.467277120371789E-5 1.315881130864954E-4 46-47 1.1333334649214464 1.583169485571898E-4 0.0 2.467277120371789E-5 1.3570024162044839E-4 48-49 1.4753659239257866 1.6448514135811927E-4 0.0 2.467277120371789E-5 1.3570024162044839E-4 50-51 1.8662114046924156 1.7270939842602522E-4 0.0 2.467277120371789E-5 1.398123701544014E-4 52-53 2.2949378135138194 1.788775912269547E-4 0.0 2.467277120371789E-5 1.4186843442137788E-4 54-55 2.767001944954554 1.8504578402788417E-4 0.0 2.467277120371789E-5 1.4392449868835436E-4 56-57 3.2968455944258617 2.0766249096462557E-4 0.0 2.467277120371789E-5 1.4598056295533085E-4 58-59 3.886078660248786 2.241110051004375E-4 0.0 2.467277120371789E-5 1.5214875575626032E-4 60-61 4.518347207243794 2.3439132643531996E-4 0.0 2.467277120371789E-5 1.603730128241663E-4 62-63 5.182291480335842 2.3850345496927294E-4 0.0 2.467277120371789E-5 1.6242907709114278E-4 64-65 5.872251134597944 2.4055951923624943E-4 0.0 2.467277120371789E-5 1.8504578402788417E-4 66-67 6.6018290912204165 2.5495196910508487E-4 0.0 2.467277120371789E-5 1.8710184829486066E-4 68-69 7.372577678724825 2.6523229043996734E-4 0.0 2.467277120371789E-5 1.9121397682881367E-4 70-71 8.17660366639025 2.7140048324089677E-4 0.0 2.467277120371789E-5 2.0560642669764908E-4 72-73 9.007457180611183 2.899050616436852E-4 0.0 2.467277120371789E-5 2.0560642669764908E-4 74-75 9.857002375083198 2.960732544446147E-4 0.0 2.467277120371789E-5 2.1177461949857857E-4 76-77 10.728615307332674 2.960732544446147E-4 0.0 2.467277120371789E-5 2.1383068376555506E-4 78-79 11.62519708404031 3.0224144724554417E-4 0.0 2.8784899737670873E-5 2.1383068376555506E-4 80-81 12.539905123325614 3.1252176858042664E-4 0.0 2.8784899737670873E-5 2.1794281229950803E-4 82-83 13.469219443163517 3.207460256483326E-4 0.0 2.8784899737670873E-5 2.22054940833461E-4 84-85 14.410154638238371 3.330824112501915E-4 0.0 2.8784899737670873E-5 2.3027919790136696E-4 86-87 15.360315393679148 3.371945397841445E-4 0.0 2.8784899737670873E-5 2.4055951923624943E-4 88 16.07829097964307 3.4541879685205044E-4 0.0 2.8784899737670873E-5 2.4261558350322591E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 160505 0.0 84.92424 8 AGTGGTA 161210 0.0 84.22699 5 TGGTATC 162750 0.0 83.72608 7 GTATCAA 162925 0.0 83.564606 9 GTGGTAT 163300 0.0 83.11454 6 GCAGTGG 164365 0.0 82.74339 3 AAGCAGT 162005 0.0 82.206345 1 CAGTGGT 171120 0.0 79.214874 4 AGCAGTG 173215 0.0 78.37479 2 CAACGCA 157435 0.0 44.52911 12-13 ATCAACG 158620 0.0 44.26306 10-11 ACGCAGA 160425 0.0 43.849773 14-15 AGAGTAC 162240 0.0 43.251263 18-19 TCAACGC 157390 0.0 43.197956 12-13 AACGCAG 159060 0.0 42.863873 14-15 GAGTACT 158385 0.0 42.724503 20-21 CGCAGAG 161375 0.0 42.273846 16-17 TATCAAC 162190 0.0 41.915264 10-11 AGTACTT 167005 0.0 41.752148 20-21 GTACTTT 167275 0.0 40.431004 22-23 >>END_MODULE