FastQCFastQC Report
Thu 26 May 2016
SRR1660727_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660727_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33518895
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT22639026.754106900003714No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA525800.15686674635306444No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT394660.11774254491384636No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA2799850.087.238448
AGTGGTA2828250.086.3732455
TGGTATC2819200.086.292997
GTGGTAT2829450.086.289546
GTATCAA2837300.085.980799
AAGCAGT2909200.084.547041
GCAGTGG2942100.083.665783
CAGTGGT2959600.082.500384
AGCAGTG3023550.081.469652
CAACGCA2769300.045.2499812-13
ATCAACG2778900.045.1688510-11
ACGCAGA2792700.044.84528414-15
TCAACGC2761800.044.3745612-13
AGAGTAC2797500.044.10421418-19
AACGCAG2791850.043.98998314-15
CGCAGAG2808200.043.6373916-17
GAGTACT2754750.043.4943120-21
TATCAAC2835750.042.9497110-11
AGTACTT2861350.042.81363720-21
GTACTTT2863950.041.84728622-23
Produced by FastQC (version 0.11.5)