##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660727_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 33518895 Sequences flagged as poor quality 0 Sequence length 100 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.666371698709042 34.0 31.0 34.0 30.0 34.0 2 31.78239440769154 34.0 31.0 34.0 30.0 34.0 3 31.84362091888769 34.0 31.0 34.0 30.0 34.0 4 35.22479643198262 37.0 35.0 37.0 33.0 37.0 5 35.19072472406981 37.0 35.0 37.0 33.0 37.0 6 35.27558808248303 37.0 35.0 37.0 33.0 37.0 7 35.20828112024576 37.0 35.0 37.0 33.0 37.0 8 35.231090404382364 37.0 35.0 37.0 32.0 37.0 9 36.887998694467704 39.0 37.0 39.0 33.0 39.0 10-11 36.796524960026275 39.0 37.0 39.0 33.0 39.0 12-13 36.848466603687264 39.0 37.0 39.0 33.0 39.0 14-15 38.21080386152347 40.0 38.0 41.0 33.0 41.0 16-17 38.1862574676164 40.0 38.0 41.0 33.0 41.0 18-19 38.142543004475534 40.0 38.0 41.0 33.0 41.0 20-21 38.009876220561566 40.0 38.0 41.0 32.5 41.0 22-23 37.81167692133049 40.0 37.5 41.0 32.0 41.0 24-25 37.81459799017838 40.0 38.0 41.0 32.0 41.0 26-27 37.82396513966228 40.0 38.0 41.0 32.0 41.0 28-29 37.756897564791444 40.0 38.0 41.0 32.0 41.0 30-31 37.67309865077593 40.0 38.0 41.0 32.0 41.0 32-33 37.55371893972041 40.0 38.0 41.0 31.0 41.0 34-35 37.40525919783454 40.0 37.0 41.0 31.0 41.0 36-37 37.210206183706234 39.5 37.0 41.0 30.0 41.0 38-39 36.731630219313615 39.0 35.0 41.0 30.0 41.0 40-41 36.674077382324214 39.0 35.0 41.0 30.0 41.0 42-43 36.676480489586545 39.0 35.0 41.0 30.0 41.0 44-45 36.68207308146644 39.5 35.0 41.0 30.0 41.0 46-47 36.77797498992732 40.0 35.0 41.0 30.0 41.0 48-49 36.679667945497606 40.0 35.0 41.0 30.0 41.0 50-51 35.33335284471639 38.5 34.0 40.0 27.5 40.5 52-53 35.48750060227224 38.5 34.5 39.5 27.5 40.5 54-55 35.7959370975684 39.0 35.0 41.0 25.5 41.0 56-57 35.363773298612614 39.0 35.0 41.0 22.5 41.0 58-59 35.183109302976725 39.0 35.0 41.0 22.0 41.0 60-61 34.98658493962883 39.0 35.0 41.0 22.0 41.0 62-63 34.779597000438116 38.5 34.0 41.0 22.0 41.0 64-65 34.55136884733223 38.0 34.0 40.5 21.0 41.0 66-67 34.31299604297814 37.0 34.0 40.0 21.0 41.0 68-69 34.06698766471866 37.0 34.0 40.0 21.0 41.0 70-71 33.79595007532319 36.0 33.5 39.0 21.0 41.0 72-73 33.47961439361292 36.0 33.0 39.0 20.5 41.0 74-75 33.119304992005254 35.0 33.0 39.0 20.0 40.5 76-77 32.732486035115414 35.0 33.0 37.5 20.0 40.0 78-79 32.36385486454729 35.0 32.5 37.0 19.0 39.0 80-81 32.04353504195171 35.0 32.0 36.5 18.5 39.0 82-83 31.734154034612416 35.0 32.0 36.0 18.0 38.0 84-85 31.451600343627078 35.0 32.0 36.0 17.5 37.0 86-87 31.23942779139945 35.0 32.0 35.0 17.0 37.0 88-89 31.03675406065743 35.0 31.0 35.0 17.0 36.5 90-91 30.864431405032892 35.0 31.0 35.0 15.0 36.0 92-93 30.712238171932576 35.0 31.0 35.0 15.0 36.0 94-95 30.531919026566953 35.0 31.0 35.0 14.5 36.0 96-97 30.401362350996358 35.0 31.0 35.0 12.5 35.0 98-99 30.30691166877667 35.0 31.0 35.0 11.5 35.0 100 30.235222790011424 34.0 30.0 35.0 11.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5357355113739324 1101 2 0.5665310550020486 1101 3 0.5549593909063724 1101 4 0.4770870274472685 1101 5 0.49544338627531914 1101 6 0.4534421710636778 1101 7 0.5020236168363823 1101 8 0.504335065256555 1101 9 0.5771716870331076 1101 10-11 0.6023411975498689 1101 12-13 0.5976114354785125 1101 14-15 0.6997758132410326 1101 16-17 0.6681200300674774 1101 18-19 0.6713223622481905 1101 20-21 0.7193418085182088 1101 22-23 0.7623592250885523 1101 24-25 0.7709225986134243 1101 26-27 0.7422104098306406 1101 28-29 0.7186365601108733 1101 30-31 0.6955916620099245 1101 32-33 0.6920428876539262 1101 34-35 0.7071904823816411 1101 36-37 0.6813334675134897 1101 38-39 0.6797385101118323 1101 40-41 0.6661361220266215 1101 42-43 0.7039427678490711 1101 44-45 0.7187502732238471 1101 46-47 0.7607509667262136 1101 48-49 0.7851790272381365 1101 50-51 0.7277971112042252 1101 52-53 0.7561283154119636 1101 54-55 0.801327745282137 1101 56-57 0.8314602963202233 1101 58-59 0.7082857349282392 1101 60-61 0.7113391783865239 1101 62-63 0.7162489046640275 1101 64-65 0.6919477779728709 1101 66-67 0.6742440997032944 1101 68-69 0.6726132488243763 1101 70-71 0.6398222927414423 1101 72-73 0.604027939658657 1101 74-75 0.6034315096907292 1101 76-77 0.5772515075721785 1101 78-79 0.6015162313220728 1101 80-81 0.5072666471087217 1101 82-83 0.513585491604001 1101 84-85 0.46449968085711646 1101 86-87 0.3896240461198275 1101 88-89 0.4292556226548747 1101 90-91 0.42061967533456723 1101 92-93 0.4380787277548883 1101 94-95 0.47406477748573295 1101 96-97 0.46600775484119694 1101 98-99 0.4404061083859183 1101 100 0.41936802167301224 1102 1 0.4944825331828149 1102 2 0.5443091161715508 1102 3 0.5239762463368329 1102 4 0.4898657081141522 1102 5 0.520832389141674 1102 6 0.4451590591977137 1102 7 0.4675203599378577 1102 8 0.49080870439302515 1102 9 0.5569953855546856 1102 10-11 0.5664848167747678 1102 12-13 0.5673323565118125 1102 14-15 0.6579703152175256 1102 16-17 0.6450954460892504 1102 18-19 0.6304764770020412 1102 20-21 0.6554522299600976 1102 22-23 0.6961975175269615 1102 24-25 0.6428677837286756 1102 26-27 0.6403217256870022 1102 28-29 0.6609529546348583 1102 30-31 0.6293782241435153 1102 32-33 0.6431660417804892 1102 34-35 0.6278676734503961 1102 36-37 0.6385221488357615 1102 38-39 0.6399082544550154 1102 40-41 0.6469889124392552 1102 42-43 0.662565106505113 1102 44-45 0.65300041759793 1102 46-47 0.6789040015243941 1102 48-49 0.7014027555680471 1102 50-51 0.6590172060725621 1102 52-53 0.7083924919547471 1102 54-55 0.7661993401798597 1102 56-57 0.7674057002023105 1102 58-59 0.6745531783574918 1102 60-61 0.7088480744575278 1102 62-63 0.6661927449684626 1102 64-65 0.6372866432909134 1102 66-67 0.6344789076213644 1102 68-69 0.5729838626255486 1102 70-71 0.6162121150839752 1102 72-73 0.6206696513309424 1102 74-75 0.5748184025784724 1102 76-77 0.6100813543213803 1102 78-79 0.5895767965147556 1102 80-81 0.5442930691376802 1102 82-83 0.5286518590752927 1102 84-85 0.5229623598027082 1102 86-87 0.4539415258733115 1102 88-89 0.4352921012850679 1102 90-91 0.44652281744375344 1102 92-93 0.4618514517631276 1102 94-95 0.49054128437583344 1102 96-97 0.4908497097024558 1102 98-99 0.4504469753520226 1102 100 0.4433772512003742 1103 1 0.4007286349865318 1103 2 0.44130780935762104 1103 3 0.4302659385669223 1103 4 0.38435377368062973 1103 5 0.3920300663976377 1103 6 0.40431960799094924 1103 7 0.39593153256493707 1103 8 0.42403958395767916 1103 9 0.47700167104800784 1103 10-11 0.45990496364941436 1103 12-13 0.4762085426156091 1103 14-15 0.5577321532019823 1103 16-17 0.5415460235313745 1103 18-19 0.5368245440284554 1103 20-21 0.5354312303455373 1103 22-23 0.5923176981687917 1103 24-25 0.6231251924375769 1103 26-27 0.5908424738363678 1103 28-29 0.5948894525463331 1103 30-31 0.5507859150196239 1103 32-33 0.48773736859133265 1103 34-35 0.5134554010373122 1103 36-37 0.5281246712282268 1103 38-39 0.5733913461251063 1103 40-41 0.530614740634995 1103 42-43 0.5643495447154123 1103 44-45 0.5998295313081243 1103 46-47 0.5981955626398161 1103 48-49 0.6376749150911607 1103 50-51 0.5563818832769414 1103 52-53 0.6003425633006003 1103 54-55 0.6574859044085031 1103 56-57 0.6697565608966372 1103 58-59 0.6001552038099689 1103 60-61 0.6166649499647079 1103 62-63 0.6008978635536764 1103 64-65 0.5391531871486706 1103 66-67 0.5432480492061345 1103 68-69 0.5414515729228171 1103 70-71 0.49005280368587023 1103 72-73 0.4631517673074157 1103 74-75 0.4749125998850374 1103 76-77 0.49997579219010646 1103 78-79 0.473549926783555 1103 80-81 0.41128137438838763 1103 82-83 0.4132150310527969 1103 84-85 0.43423945756953586 1103 86-87 0.38001459396407 1103 88-89 0.38903347747009676 1103 90-91 0.3950017252896849 1103 92-93 0.3213601609258845 1103 94-95 0.39781913965036253 1103 96-97 0.3685757782968402 1103 98-99 0.3703473723323185 1103 100 0.369747931808071 1104 1 0.3644133617585972 1104 2 0.37975416013602725 1104 3 0.38269885850078467 1104 4 0.29625352594757715 1104 5 0.310036856809738 1104 6 0.26842304462471844 1104 7 0.24878202918090153 1104 8 0.3023584920258173 1104 9 0.3808993089351347 1104 10-11 0.33926473050770767 1104 12-13 0.34639718702285194 1104 14-15 0.40830205921806595 1104 16-17 0.4046170335643424 1104 18-19 0.3806441991619067 1104 20-21 0.3319377376293744 1104 22-23 0.42172464196008264 1104 24-25 0.44458198146653416 1104 26-27 0.40000389760320587 1104 28-29 0.3815621939444256 1104 30-31 0.3450017997312713 1104 32-33 0.34599336519445956 1104 34-35 0.33023692434398555 1104 36-37 0.35891856019062374 1104 38-39 0.3410502004072029 1104 40-41 0.37065587505443176 1104 42-43 0.3687889757659377 1104 44-45 0.3648916045535202 1104 46-47 0.40939057718284033 1104 48-49 0.4583682743587474 1104 50-51 0.4044464625156934 1104 52-53 0.4053891786431194 1104 54-55 0.47927368186669383 1104 56-57 0.49472787312195265 1104 58-59 0.44580591053075835 1104 60-61 0.44768727460266433 1104 62-63 0.434176682060631 1104 64-65 0.3847703650848686 1104 66-67 0.43482385875463336 1104 68-69 0.3857906242581919 1104 70-71 0.3738790645898078 1104 72-73 0.35551850288695164 1104 74-75 0.32956358556798193 1104 76-77 0.3103038243537384 1104 78-79 0.3202536239819693 1104 80-81 0.2905674845727759 1104 82-83 0.29706102261327416 1104 84-85 0.29354047906428704 1104 86-87 0.2529483659490026 1104 88-89 0.2609712845742642 1104 90-91 0.29013485222879254 1104 92-93 0.3249534067755313 1104 94-95 0.37292891642398374 1104 96-97 0.3241545714405696 1104 98-99 0.3221166822959347 1104 100 0.2770076469067213 1105 1 0.2905625384364221 1105 2 0.3276816184599518 1105 3 0.32563379890457966 1105 4 0.29261848702520155 1105 5 0.28997992366921466 1105 6 0.2586326152914822 1105 7 0.277765993876514 1105 8 0.2802639849929207 1105 9 0.2892741224205082 1105 10-11 0.2978089786467706 1105 12-13 0.31078385700685374 1105 14-15 0.36605423314090046 1105 16-17 0.3441642425580014 1105 18-19 0.35328475087245437 1105 20-21 0.3894450642283971 1105 22-23 0.42371515494379963 1105 24-25 0.4253207226689 1105 26-27 0.3971201426674398 1105 28-29 0.3815528207793406 1105 30-31 0.3942691220019867 1105 32-33 0.3728411116104269 1105 34-35 0.3719586103486989 1105 36-37 0.362752125112749 1105 38-39 0.4177189546176052 1105 40-41 0.4004164463592801 1105 42-43 0.4516677671314966 1105 44-45 0.4614232971337131 1105 46-47 0.47316779584417645 1105 48-49 0.5141733583875308 1105 50-51 0.3655870366328031 1105 52-53 0.4155231735440381 1105 54-55 0.5137571731517596 1105 56-57 0.5147707067851073 1105 58-59 0.43764795934389156 1105 60-61 0.4285382752723379 1105 62-63 0.4271977168346268 1105 64-65 0.39195141263269306 1105 66-67 0.3903240958954015 1105 68-69 0.3950758572099673 1105 70-71 0.3763110856362246 1105 72-73 0.33959982467499117 1105 74-75 0.3167402486831108 1105 76-77 0.314295998591156 1105 78-79 0.3379294991593653 1105 80-81 0.2690053001727932 1105 82-83 0.26808256456300583 1105 84-85 0.290036936958348 1105 86-87 0.24421002728031738 1105 88-89 0.28980185372036615 1105 90-91 0.2890831237161997 1105 92-93 0.2721252607571252 1105 94-95 0.3026283823532374 1105 96-97 0.2767701816284749 1105 98-99 0.2514587877748937 1105 100 0.29122285907586587 1106 1 0.231361153664011 1106 2 0.2854961561882483 1106 3 0.2692110662701417 1106 4 0.2526046856522086 1106 5 0.24872152401887604 1106 6 0.24743004208381336 1106 7 0.27461904263010695 1106 8 0.28245366652868853 1106 9 0.2750704094140275 1106 10-11 0.24900639658795143 1106 12-13 0.2813718718619356 1106 14-15 0.3280621408762059 1106 16-17 0.31501974917567566 1106 18-19 0.28528688181494744 1106 20-21 0.3345602178261373 1106 22-23 0.301946545726139 1106 24-25 0.29243589308705253 1106 26-27 0.35034585224318704 1106 28-29 0.3185453945672023 1106 30-31 0.2904986477908622 1106 32-33 0.2980314187193187 1106 34-35 0.3075102167530659 1106 36-37 0.2928345300522821 1106 38-39 0.3144978579910642 1106 40-41 0.32870533739379937 1106 42-43 0.346483296049513 1106 44-45 0.3678746328163669 1106 46-47 0.37483254695828094 1106 48-49 0.38727168540177814 1106 50-51 0.2934909232757974 1106 52-53 0.3553279165308183 1106 54-55 0.516019317162943 1106 56-57 0.515543151995125 1106 58-59 0.45087045571428064 1106 60-61 0.39696417346005575 1106 62-63 0.4165230567648308 1106 64-65 0.4025560610452317 1106 66-67 0.37706855161658837 1106 68-69 0.3465790186732818 1106 70-71 0.4144397804316782 1106 72-73 0.39782095126396655 1106 74-75 0.4105000848755509 1106 76-77 0.3710187715226212 1106 78-79 0.3858648974234171 1106 80-81 0.35039893398282373 1106 82-83 0.38454058327993934 1106 84-85 0.3468048521468994 1106 86-87 0.2890639570505158 1106 88-89 0.3446937187016843 1106 90-91 0.3244628568195438 1106 92-93 0.2997892858419 1106 94-95 0.33867869412983254 1106 96-97 0.3828884273787203 1106 98-99 0.32161046690999484 1106 100 0.3159237068586229 1107 1 0.20258383882981335 1107 2 0.2500144315230379 1107 3 0.21749459649019443 1107 4 0.21759975772209827 1107 5 0.24295546374475663 1107 6 0.19618740288989045 1107 7 0.21698545367661382 1107 8 0.22802035124755093 1107 9 0.24329056598285348 1107 10-11 0.2396536382799681 1107 12-13 0.2314760334908641 1107 14-15 0.2610222847546382 1107 16-17 0.24339569433461605 1107 18-19 0.24942905161307038 1107 20-21 0.26261704711328093 1107 22-23 0.2770921511241582 1107 24-25 0.28048748055370254 1107 26-27 0.2588089223476615 1107 28-29 0.2552670547922773 1107 30-31 0.20347434969851008 1107 32-33 0.1650200753912756 1107 34-35 0.20848974914273555 1107 36-37 0.22093629767054068 1107 38-39 0.26668398202276933 1107 40-41 0.2478808654984661 1107 42-43 0.29465445450600924 1107 44-45 0.286319427816089 1107 46-47 0.26404969253398036 1107 48-49 0.3190869250589117 1107 50-51 0.22306825172469047 1107 52-53 0.24114468703840686 1107 54-55 0.3147071538886337 1107 56-57 0.3586746008152417 1107 58-59 0.30596479553776845 1107 60-61 0.31226553650568434 1107 62-63 0.30659354349050005 1107 64-65 0.30620918710608436 1107 66-67 0.32954300103441625 1107 68-69 0.29595876469785054 1107 70-71 0.254531673407719 1107 72-73 0.2334171212005316 1107 74-75 0.25870612445121566 1107 76-77 0.2464814427395865 1107 78-79 0.25777972418231343 1107 80-81 0.20465171470923593 1107 82-83 0.23558086173493464 1107 84-85 0.21637380862201283 1107 86-87 0.20662947062763237 1107 88-89 0.18512541346228417 1107 90-91 0.21780900774180978 1107 92-93 0.23808165009824833 1107 94-95 0.24702270420153383 1107 96-97 0.2929044903208329 1107 98-99 0.24948039407452782 1107 100 0.23557847687692757 1108 1 0.08160694449970052 1108 2 0.13823417525485837 1108 3 0.15617267565274418 1108 4 0.13241361049019673 1108 5 0.1326618921381666 1108 6 0.0718846132595985 1108 7 0.06827477835074802 1108 8 0.10982721745246238 1108 9 0.11599683314963016 1108 10-11 0.08221858677774918 1108 12-13 0.10746609424113274 1108 14-15 0.1488874289214266 1108 16-17 0.11695744824270093 1108 18-19 0.08353758409533896 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-1.1888278051753112 2316 84-85 -1.267508122148275 2316 86-87 -1.4223618534927809 2316 88-89 -1.459334939806542 2316 90-91 -1.3180255447343505 2316 92-93 -1.180399789728849 2316 94-95 -1.3747527197017142 2316 96-97 -1.5837881115528383 2316 98-99 -1.4229802233363031 2316 100 -1.2007496359707837 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 17530.0 3 4381.0 4 3153.0 5 2607.0 6 5385.0 7 33542.0 8 62116.0 9 67154.0 10 67169.0 11 64417.0 12 62796.0 13 66405.0 14 74018.0 15 83791.0 16 95901.0 17 110011.0 18 124355.0 19 141309.0 20 161767.0 21 195137.0 22 248984.0 23 356988.0 24 551366.0 25 708234.0 26 687920.0 27 568961.0 28 493094.0 29 469310.0 30 509953.0 31 608904.0 32 748881.0 33 960090.0 34 1319696.0 35 2035129.0 36 3513331.0 37 6629102.0 38 8819396.0 39 2840581.0 40 6031.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 15.901018682155321 35.060789761383845 27.46559171142526 21.572599845035572 2 20.136279906920045 35.17117410897409 26.59895519856163 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26.020248041768046 19.865151775019793 22-23 23.83080263575104 26.354786830920673 29.912495269383992 19.901915263944296 24-25 19.949600670601463 30.11261385721457 25.917556220812983 24.020229251370985 26-27 19.97769731270818 26.199203169168 33.87638272204494 19.946716796078878 28-29 19.967563182115263 26.27989229516722 33.86289438842244 19.889650134295074 30-31 19.932781763090514 26.21884702325316 33.854061407805936 19.994309805850385 32-33 20.03265781530863 26.333565910138745 33.67694521752277 19.95683105702985 34-35 20.05465338978961 26.437241573251157 33.57129818689728 19.936806850061952 36-37 19.98941039201633 26.392688819252143 33.636173006641584 19.981727782089944 38-39 20.014378107647513 26.386548159642537 33.640568512377286 19.95850522033267 40-41 20.027801448287256 26.411438588318838 33.636441949193554 19.924318014200352 42-43 20.01686437223217 26.43476838818787 33.603870013845466 19.94449722573449 44-45 20.018335429755684 26.485229294300904 33.578415249195125 19.918020026748287 46-47 19.99953836977286 26.56250634517615 33.52723295855886 19.910722326492127 48-49 20.049297453800992 26.547352236413975 33.449979687084046 19.953370622700987 50-51 20.033673771666844 26.628731436858533 33.39376910990947 19.94382568156515 52-53 20.124150033688053 26.763257503356858 33.11045261452744 20.002139848427653 54-55 20.48069935436413 27.098046292758692 32.21882405136415 20.20243030151302 56-57 20.96382180603627 27.72801391095212 30.896710099366885 20.41145418364472 58-59 21.018742754314975 28.32897722383862 30.174154184384317 20.478125837462088 60-61 21.045243826866862 28.642081315995306 29.826755854051406 20.485919003086423 62-63 21.007173241204434 28.814844958085782 29.674778220883862 20.50320357982592 64-65 21.028832087104895 28.864216910146194 29.653251987607938 20.453699015140973 66-67 21.000284644029627 28.6669276824638 29.911491807440598 20.42129586606598 68-69 20.98297990350863 28.478549424982898 30.193752414752506 20.34471825675597 70-71 20.952632434789844 28.337861456966813 30.427086455459378 20.28241965278396 72-73 20.986488196054033 28.240995362722447 30.489885236740584 20.282631204482936 74-75 21.07392968751528 28.317380719558656 30.3712108917715 20.237478701154565 76-77 21.248100953490887 28.470301263981046 30.054451293034706 20.227146489493364 78-79 21.38705657723112 28.661485562870563 29.68970469811066 20.261753161787656 80-81 21.530400787595717 28.88832265882814 29.336808525117053 20.24446802845909 82-83 21.609764955730114 29.089700556111996 29.021300228946885 20.279234259211 84-85 21.650353907616587 29.184721769354837 28.830435563682038 20.334488759346538 86-87 21.741790293761948 29.25567851382931 28.676000441163307 20.326530751245432 88-89 21.87679400679243 29.330689077859596 28.486425896316263 20.306091019031708 90-91 21.99123583524838 29.340855868989063 28.35006029006425 20.31784800569831 92-93 22.09088329891703 29.412683774869457 28.24455016783101 20.251882758382497 94-95 22.193425407922724 29.44807914415939 28.146573306893153 20.21192214102473 96-97 22.26368704833495 29.426762396135608 28.093692782322645 20.2158577732068 98-99 22.34222990400431 29.422170831112005 28.041836796662217 20.193762468221465 100 22.372144450850207 29.456210206041522 27.93645768873514 20.235187654373128 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 72364.0 1 49662.0 2 22171.5 3 16268.5 4 14760.0 5 14418.0 6 14507.5 7 14514.5 8 14305.5 9 14244.5 10 15209.5 11 20016.5 12 189032.5 13 233071.5 14 108216.5 15 111149.0 16 125377.5 17 138886.5 18 150121.0 19 161636.5 20 176058.5 21 196747.5 22 226626.5 23 260803.5 24 295550.0 25 334259.5 26 380884.5 27 437046.0 28 501845.5 29 566904.5 30 622435.0 31 672151.5 32 725704.5 33 776829.5 34 819377.0 35 863585.5 36 914885.0 37 957820.5 38 997298.5 39 1023415.5 40 1026223.5 41 1039782.5 42 1065195.5 43 1067523.0 44 1057929.5 45 1061871.0 46 1063899.0 47 1060812.0 48 1045635.0 49 1022398.5 50 1005604.0 51 984506.5 52 953482.0 53 905270.5 54 843433.5 55 781638.5 56 718399.0 57 650106.5 58 576971.0 59 497603.0 60 415627.5 61 341104.0 62 270807.0 63 204902.5 64 151924.0 65 112178.5 66 82450.5 67 61757.0 68 46675.0 69 35711.0 70 28177.5 71 22609.0 72 18876.0 73 15600.5 74 12755.0 75 10790.5 76 8728.0 77 6495.5 78 4974.5 79 3959.5 80 3232.5 81 2728.5 82 2292.5 83 1980.5 84 1661.5 85 1368.5 86 1177.0 87 1028.5 88 865.5 89 724.0 90 622.5 91 551.0 92 481.5 93 396.5 94 333.0 95 291.0 96 246.5 97 192.5 98 160.5 99 112.0 100 59.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.40884402663035285 2 0.38774249568787994 3 0.16997875377455016 4 0.17624686016648222 5 0.277443513576447 6 0.10380712132664277 7 0.13813999536679236 8 0.14393374244586524 9 0.1365916149682142 10-11 0.1332472326429615 12-13 0.13483588883225417 14-15 0.14145901886085444 16-17 0.14577897033896853 18-19 0.1570248661240175 20-21 0.13791027419012472 22-23 0.1001300908040077 24-25 0.0952940125263676 26-27 0.08981650498920087 28-29 0.09991230319495914 30-31 0.10434711526140703 32-33 0.10541069447545928 34-35 0.10596709706569982 36-37 0.1019738270011586 38-39 0.10131598908615573 40-41 0.1023557011649698 42-43 0.06459192643432905 44-45 0.06725609540529304 46-47 0.08618124195323265 48-49 0.09054594430991833 50-51 0.09990335301924481 52-53 0.10506014592664825 54-55 0.10743940097070621 56-57 0.10803458765570881 58-59 0.10750951068046843 60-61 0.10534505985355423 62-63 0.10984848993381197 64-65 0.1165849888547937 66-67 0.1252845596491173 68-69 0.13029516635318675 70-71 0.12860954992698895 72-73 0.1157511308174091 74-75 0.11311082898168331 76-77 0.11517831957169233 78-79 0.11394021193121073 80-81 0.10810171397356626 82-83 0.11137001980524716 84-85 0.1173203949593207 86-87 0.13056217992866412 88-89 0.13823546390774516 90-91 0.14505400610610822 92-93 0.14416644701443768 94-95 0.13452710777010995 96-97 0.13107979842414255 98-99 0.12616167686912114 100 0.12038583014147691 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3.3518895E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.18927295872932 #Duplication Level Percentage of deduplicated Percentage of total 1 68.53591748315411 28.229446126905835 2 16.157190272455132 13.310058407565611 3 6.097251170263169 7.534240282497041 4 2.813443818030582 4.635348214996449 5 1.5673414023975638 3.2278826421435434 6 1.0082556753246301 2.491759093388049 7 0.6834347333671895 1.9705125847496292 8 0.47177713932559084 1.5545725893896185 9 0.3707103807131489 1.3742361953845519 >10 2.0167743985245687 14.771208934936666 >50 0.1521829456894076 4.496097664564074 >100 0.12466308415179221 8.533747814305448 >500 4.410008862528007E-4 0.12204721768610896 >1k 5.22602236681346E-4 0.5048729453464896 >5k 6.500317880135456E-5 0.1728075189350051 >10k+ 2.8890301417562E-5 7.07116176720586 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 2263902 6.754106900003714 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 52580 0.15686674635306444 No Hit AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 39466 0.11774254491384636 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 5.549108942881321E-4 1.4916959523874519E-5 0.0 0.0 5.966783809549808E-6 2 5.966783809549808E-4 3.878409476207375E-5 0.0 0.0 5.966783809549808E-6 3 6.235289080979549E-4 5.370105428594827E-5 0.0 0.0 5.966783809549808E-6 4 6.473960433361542E-4 7.75681895241475E-5 0.0 0.0 5.966783809549808E-6 5 6.50379435240929E-4 9.248514904802202E-5 0.0 2.983391904774904E-6 5.966783809549808E-6 6 6.533628271457039E-4 1.0441871666712163E-4 0.0 2.983391904774904E-6 5.966783809549808E-6 7 6.623130028600286E-4 1.1336889238144634E-4 0.0 2.983391904774904E-6 8.950175714324712E-6 8 6.861801380982278E-4 1.1336889238144634E-4 0.0 2.983391904774904E-6 8.950175714324712E-6 9 7.040804895268773E-4 1.1933567619099615E-4 0.0 8.950175714324712E-6 8.950175714324712E-6 10-11 7.204891450031392E-4 1.2530246000054597E-4 1.491695952387452E-6 8.950175714324712E-6 1.1933567619099615E-5 12-13 7.577815438128256E-4 1.491695952387452E-4 2.983391904774904E-6 1.0441871666712164E-5 1.3425263571487067E-5 14-15 7.965656385748993E-4 1.640865547626197E-4 2.983391904774904E-6 1.4916959523874519E-5 1.4916959523874519E-5 16-17 8.517583888132351E-4 1.7602012238171933E-4 2.983391904774904E-6 1.7900351428649424E-5 1.7900351428649424E-5 18-19 9.263431864326077E-4 1.954121697627562E-4 2.983391904774904E-6 1.7900351428649424E-5 1.7900351428649424E-5 20-21 9.710940650042312E-4 2.2822948071528012E-4 2.983391904774904E-6 1.9392047381036875E-5 1.7900351428649424E-5 22-23 0.001026286815242567 2.4463813619154213E-4 2.983391904774904E-6 2.0883743333424327E-5 2.6850527142974134E-5 24-25 0.0012023069376242861 2.5657170381064175E-4 2.983391904774904E-6 2.386713523819923E-5 2.6850527142974134E-5 26-27 0.0017318590007218315 2.849139269060033E-4 2.983391904774904E-6 2.8342223095361586E-5 2.6850527142974134E-5 28-29 0.0030922857092991877 3.1027275809659E-4 2.983391904774904E-6 3.2817310952523944E-5 2.9833919047749037E-5 30-31 0.006168162763122114 3.3563158928717664E-4 2.983391904774904E-6 3.2817310952523944E-5 3.2817310952523944E-5 32-33 0.012257265640767692 3.56515332620601E-4 2.983391904774904E-6 3.2817310952523944E-5 3.580070285729885E-5 34-35 0.022527592272955296 3.759073800016379E-4 2.983391904774904E-6 3.2817310952523944E-5 3.580070285729885E-5 36-37 0.038215758604214126 4.176748666684866E-4 2.983391904774904E-6 3.2817310952523944E-5 3.580070285729885E-5 38-39 0.06045097847050149 4.370669140495234E-4 2.983391904774904E-6 3.580070285729885E-5 3.580070285729885E-5 40-41 0.08927203596657945 4.385586100019109E-4 2.983391904774904E-6 3.72923988096863E-5 4.1767486666848654E-5 42-43 0.12498622045863982 4.430336978590732E-4 2.983391904774904E-6 4.02757907144612E-5 4.773427047639846E-5 44-45 0.1692015801833563 4.534755695257854E-4 2.983391904774904E-6 4.1767486666848654E-5 5.370105428594827E-5 46-47 0.22341279448502105 4.6391744119249757E-4 2.983391904774904E-6 4.773427047639846E-5 5.9667838095498075E-5 48-49 0.2888639377879253 4.937513602402467E-4 2.983391904774904E-6 5.2209358333560816E-5 5.9667838095498075E-5 50-51 0.3638156926115852 5.14635103573671E-4 2.983391904774904E-6 5.370105428594827E-5 6.115953404788553E-5 52-53 0.44772657332528415 5.295520630975454E-4 2.983391904774904E-6 5.370105428594827E-5 6.414292595266044E-5 54-55 0.5423135219702201 5.444690226214199E-4 2.983391904774904E-6 5.817614214311062E-5 7.16014057145977E-5 56-57 0.6495724277306874 5.683361578596193E-4 2.983391904774904E-6 6.265123000027298E-5 7.16014057145977E-5 58-59 0.769756878918592 5.862365092882686E-4 2.983391904774904E-6 6.265123000027298E-5 7.16014057145977E-5 60-61 0.9042511693777495 6.086119485740804E-4 2.983391904774904E-6 6.265123000027298E-5 7.16014057145977E-5 62-63 1.0488487165224272 6.280039959551172E-4 5.966783809549808E-6 6.265123000027298E-5 8.950175714324712E-5 64-65 1.2048219370000115 6.488877392885415E-4 5.966783809549808E-6 6.265123000027298E-5 8.950175714324712E-5 66-67 1.3742681553195593 6.623130028600287E-4 5.966783809549808E-6 6.265123000027298E-5 8.950175714324712E-5 68-69 1.559945517297035 6.652963947648035E-4 5.966783809549808E-6 6.712631785743534E-5 9.845193285757183E-5 70-71 1.7602981840540983 6.757382664315157E-4 5.966783809549808E-6 7.45847976193726E-5 9.994362880995928E-5 72-73 1.9737777751921715 7.010970976221024E-4 5.966783809549808E-6 7.45847976193726E-5 1.0143532476234673E-4 74-75 2.202644210079121 7.204891450031392E-4 5.966783809549808E-6 7.75681895241475E-5 1.0143532476234673E-4 76-77 2.4466707509301844 7.324227126222389E-4 5.966783809549808E-6 7.75681895241475E-5 1.0143532476234673E-4 78-79 2.708578251162516 7.592732397652131E-4 5.966783809549808E-6 7.75681895241475E-5 1.0292702071473418E-4 80-81 2.9871211446558723 7.771735911938625E-4 5.966783809549808E-6 7.905988547653495E-5 1.0591041261950908E-4 82-83 3.2816087761842985 7.995490304796742E-4 5.966783809549808E-6 8.05515814289224E-5 1.1933567619099615E-4 84-85 3.5936074264978006 8.099909021463864E-4 5.966783809549808E-6 8.05515814289224E-5 1.267941559529334E-4 86-87 3.924622216812338 8.234161657178734E-4 5.966783809549808E-6 8.05515814289224E-5 1.3126924381009578E-4 88 4.184019192756802 8.323663414321981E-4 5.966783809549808E-6 8.05515814289224E-5 1.3126924381009578E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 279985 0.0 87.23844 8 AGTGGTA 282825 0.0 86.373245 5 TGGTATC 281920 0.0 86.29299 7 GTGGTAT 282945 0.0 86.28954 6 GTATCAA 283730 0.0 85.98079 9 AAGCAGT 290920 0.0 84.54704 1 GCAGTGG 294210 0.0 83.66578 3 CAGTGGT 295960 0.0 82.50038 4 AGCAGTG 302355 0.0 81.46965 2 CAACGCA 276930 0.0 45.24998 12-13 ATCAACG 277890 0.0 45.16885 10-11 ACGCAGA 279270 0.0 44.845284 14-15 TCAACGC 276180 0.0 44.37456 12-13 AGAGTAC 279750 0.0 44.104214 18-19 AACGCAG 279185 0.0 43.989983 14-15 CGCAGAG 280820 0.0 43.63739 16-17 GAGTACT 275475 0.0 43.49431 20-21 TATCAAC 283575 0.0 42.94971 10-11 AGTACTT 286135 0.0 42.813637 20-21 GTACTTT 286395 0.0 41.847286 22-23 >>END_MODULE