FastQCFastQC Report
Thu 26 May 2016
SRR1660727_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660727_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33518895
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT21389426.381302247583043No Hit
TAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT463080.13815491232631624No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT426230.12716111315722073No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA400400.11945501186718716No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA2758850.086.689618
AGTGGTA2771600.085.603545
TGGTATC2796350.085.4553157
GTGGTAT2776300.085.4028246
AAGCAGT2706100.085.298181
GTATCAA2801550.085.227339
GCAGTGG2797400.084.772923
AGCAGTG2865750.082.478992
CAGTGGT2903600.081.58684
CAACGCA2712150.045.049812-13
ATCAACG2722900.044.9374510-11
ACGCAGA2744100.044.5699514-15
TCAACGC2705650.044.02259412-13
AGAGTAC2779550.043.99379318-19
AACGCAG2732000.043.68409714-15
GAGTACT2732450.043.37101420-21
CGCAGAG2758800.043.26093716-17
AGTACTT2853550.042.6465520-21
TATCAAC2796750.042.6440110-11
CAGAGTA2871100.041.60432418-19
Produced by FastQC (version 0.11.5)