##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660727_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 33518895 Sequences flagged as poor quality 0 Sequence length 100 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.25254075350634 34.0 31.0 34.0 27.0 34.0 2 31.900635268555245 34.0 31.0 34.0 28.0 34.0 3 31.668019008383183 34.0 31.0 34.0 30.0 34.0 4 35.58817610186732 37.0 35.0 37.0 33.0 37.0 5 35.924582537700005 37.0 35.0 37.0 35.0 37.0 6 35.38667900597559 37.0 35.0 37.0 33.0 37.0 7 35.59432087483791 37.0 35.0 37.0 35.0 37.0 8 35.969929736645554 37.0 35.0 37.0 35.0 37.0 9 37.786247607506155 39.0 38.0 39.0 35.0 39.0 10-11 37.784039196399526 39.0 37.5 39.0 35.0 39.0 12-13 37.88124413110874 39.0 38.0 39.0 35.0 39.0 14-15 39.35137393401543 41.0 39.0 41.0 36.0 41.0 16-17 39.31806579542673 41.0 39.0 41.0 36.0 41.0 18-19 39.30745612288233 41.0 39.0 41.0 36.0 41.0 20-21 39.235251639411146 40.5 39.0 41.0 36.0 41.0 22-23 39.09382546769516 40.0 39.0 41.0 35.5 41.0 24-25 39.12120615849658 40.0 39.0 41.0 36.0 41.0 26-27 39.09995387676115 40.0 39.0 41.0 36.0 41.0 28-29 39.034081448687374 40.0 39.0 41.0 35.0 41.0 30-31 38.94850164362518 40.0 38.5 41.0 35.0 41.0 32-33 38.83705413021521 40.0 38.0 41.0 35.0 41.0 34-35 38.760972296371946 40.0 38.0 41.0 35.0 41.0 36-37 38.629064726029895 40.0 38.0 41.0 35.0 41.0 38-39 38.18284105129361 40.0 37.0 41.0 34.0 41.0 40-41 38.06005554479049 40.0 37.0 41.0 34.0 41.0 42-43 37.905718207596045 40.0 36.5 41.0 33.0 41.0 44-45 37.78999264444725 40.0 36.0 41.0 33.0 41.0 46-47 37.65284599924908 40.0 36.0 41.0 33.0 41.0 48-49 37.702242854366176 40.0 36.0 41.0 33.0 41.0 50-51 37.70377545262157 40.0 36.0 41.0 33.0 41.0 52-53 37.4410103465523 40.0 36.0 41.0 33.0 41.0 54-55 36.86323085829649 40.0 35.5 41.0 30.5 41.0 56-57 36.3037565379169 39.5 35.0 41.0 28.5 41.0 58-59 36.15788048800535 39.0 35.0 41.0 28.0 41.0 60-61 36.03121297703878 39.0 35.0 41.0 28.0 41.0 62-63 35.8134692238512 39.0 35.0 41.0 28.0 41.0 64-65 35.56510884383271 38.5 35.0 40.5 28.0 41.0 66-67 35.303949250116986 37.5 35.0 40.0 27.5 41.0 68-69 35.05551632892433 37.0 35.0 40.0 27.0 41.0 70-71 34.77321158707649 36.5 34.5 39.5 27.0 41.0 72-73 34.44859966296622 36.0 34.0 39.0 26.0 41.0 74-75 34.080114678601426 35.5 34.0 39.0 26.0 40.5 76-77 32.37722614364227 34.5 32.0 36.5 24.0 39.0 78-79 33.31068377999931 35.0 33.5 37.0 26.0 39.0 80-81 33.20767605853355 35.0 34.0 37.0 26.0 39.0 82-83 32.944336261681656 35.0 34.0 36.0 25.0 38.5 84-85 32.6902176966156 35.0 34.0 36.0 25.0 37.0 86-87 32.473975663577214 35.0 34.0 35.5 25.0 37.0 88-89 32.29789095076076 35.0 33.5 35.0 25.0 36.5 90-91 32.132574075010524 35.0 33.0 35.0 24.0 36.0 92-93 31.986750219540355 35.0 33.0 35.0 24.0 36.0 94-95 31.878378165509332 35.0 33.0 35.0 24.0 36.0 96-97 31.776023105773625 35.0 33.0 35.0 23.0 36.0 98-99 31.687229725204247 35.0 33.0 35.0 23.0 35.0 100 31.637279958065445 35.0 33.0 35.0 21.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.5655718054488759 1101 2 1.1703861799040958 1101 3 1.5278188152543635 1101 4 0.9424111978318521 1101 5 0.5604376294804467 1101 6 1.114120526248513 1101 7 0.8131214917843579 1101 8 0.47685190778832975 1101 9 0.5059401545815874 1101 10-11 0.40770076471940087 1101 12-13 0.3786408400999832 1101 14-15 0.49415803965275984 1101 16-17 0.5332759594279963 1101 18-19 0.503335231367295 1101 20-21 0.5105006641730938 1101 22-23 0.5139450799009353 1101 24-25 0.49084175616410874 1101 26-27 0.48197384175897895 1101 28-29 0.46395693344290834 1101 30-31 0.48953713969853396 1101 32-33 0.5151063992940408 1101 34-35 0.5162463388377461 1101 36-37 0.5136400964267622 1101 38-39 0.5283321434408208 1101 40-41 0.5321190237660858 1101 42-43 0.5303929005722949 1101 44-45 0.5295038048022533 1101 46-47 0.5287936764471723 1101 48-49 0.5073891758237181 1101 50-51 0.4813151767771231 1101 52-53 0.447861013864177 1101 54-55 0.45498286526258624 1101 56-57 0.5335971338847116 1101 58-59 0.5129165705323473 1101 60-61 0.505284553850835 1101 62-63 0.46396466514563883 1101 64-65 0.4493231350965843 1101 66-67 0.4588078386214107 1101 68-69 0.45523341094598635 1101 70-71 0.47718381874196325 1101 72-73 0.43302625835801223 1101 74-75 0.4281134031371252 1101 76-77 0.36214179896416 1101 78-79 0.45639567833300987 1101 80-81 0.43969287909252586 1101 82-83 0.4774549095759113 1101 84-85 0.43569632384915025 1101 86-87 0.4369163727757197 1101 88-89 0.41908896028024145 1101 90-91 0.4240212592581898 1101 92-93 0.4420146923188497 1101 94-95 0.38895520199681854 1101 96-97 0.4104890233966749 1101 98-99 0.4227835740245247 1101 100 0.41561267912027944 1102 1 1.513647830454353 1102 2 1.1444036719475967 1102 3 1.4995000261636307 1102 4 0.9060087140187179 1102 5 0.49690029023764026 1102 6 1.0584614064222748 1102 7 0.7525853097112645 1102 8 0.43079987836538436 1102 9 0.45607735260229987 1102 10-11 0.35481320606188405 1102 12-13 0.34722219917818364 1102 14-15 0.46482065514236126 1102 16-17 0.46541890696980914 1102 18-19 0.41987831365306505 1102 20-21 0.4137550321590098 1102 22-23 0.4239835561130434 1102 24-25 0.41623054157418693 1102 26-27 0.41689055792267027 1102 28-29 0.42577277813457926 1102 30-31 0.42605650353896607 1102 32-33 0.43782961164727396 1102 34-35 0.4089572540070776 1102 36-37 0.41537705099309363 1102 38-39 0.4392965855311104 1102 40-41 0.4755537735146689 1102 42-43 0.4725340422909312 1102 44-45 0.45136467020376614 1102 46-47 0.4711068761729038 1102 48-49 0.4344957424565692 1102 50-51 0.39543892645684053 1102 52-53 0.40089856244880906 1102 54-55 0.43793334085115276 1102 56-57 0.4695625060465005 1102 58-59 0.4412091979508901 1102 60-61 0.44199727414253687 1102 62-63 0.3834968477323386 1102 64-65 0.35593296091131066 1102 66-67 0.37447464865784497 1102 68-69 0.3460221714157612 1102 70-71 0.32236209861073917 1102 72-73 0.3001199225346909 1102 74-75 0.31932797056806095 1102 76-77 0.17728831431573866 1102 78-79 0.35708644621274743 1102 80-81 0.36002991491262293 1102 82-83 0.3783958843645294 1102 84-85 0.3578871174763876 1102 86-87 0.3564492043156946 1102 88-89 0.32896844293939864 1102 90-91 0.34282493423278027 1102 92-93 0.34089872240867436 1102 94-95 0.3105215887197481 1102 96-97 0.29630749562300096 1102 98-99 0.32206575845529173 1102 100 0.36727923863796974 1103 1 1.4607281878665788 1103 2 1.0753554734136337 1103 3 1.4312138190940829 1103 4 0.8837148215805044 1103 5 0.49542227286581664 1103 6 1.0477387868740635 1103 7 0.7483097952273141 1103 8 0.4237058377461409 1103 9 0.4287818500137135 1103 10-11 0.3472981741237291 1103 12-13 0.31228788317601897 1103 14-15 0.38629954500064656 1103 16-17 0.43027419670709577 1103 18-19 0.3904375280948713 1103 20-21 0.39196534710749376 1103 22-23 0.37575825167077426 1103 24-25 0.39121780777070114 1103 26-27 0.3754960064281363 1103 28-29 0.36718304242732813 1103 30-31 0.3798585174400486 1103 32-33 0.39127874624311687 1103 34-35 0.40761370970510313 1103 36-37 0.37786311228128255 1103 38-39 0.40966683565572737 1103 40-41 0.4382897074280052 1103 42-43 0.428080437684045 1103 44-45 0.3917678493053529 1103 46-47 0.42362962508227753 1103 48-49 0.3791569089339717 1103 50-51 0.3458138296633919 1103 52-53 0.35078492980601084 1103 54-55 0.3806338118667938 1103 56-57 0.4352679990385653 1103 58-59 0.3960686096034678 1103 60-61 0.40694172128075223 1103 62-63 0.36985005549054506 1103 64-65 0.3153030074467935 1103 66-67 0.3284593448192439 1103 68-69 0.32678980949609127 1103 70-71 0.32950387795291647 1103 72-73 0.3392122781483735 1103 74-75 0.3341012783820787 1103 76-77 0.20956739265980673 1103 78-79 0.3760864146920042 1103 80-81 0.4048205507890046 1103 82-83 0.38367984576559877 1103 84-85 0.3342962517645063 1103 86-87 0.32274155035685226 1103 88-89 0.30204332572462533 1103 90-91 0.3117315469287476 1103 92-93 0.3244482713908283 1103 94-95 0.31943009588630034 1103 96-97 0.331268497947665 1103 98-99 0.35427919576311595 1103 100 0.3488417389619656 1104 1 1.4171638314309405 1104 2 1.0477890799210208 1104 3 1.398332879941492 1104 4 0.8641656070906052 1104 5 0.49089075430850215 1104 6 1.0171225404781552 1104 7 0.7378123527136822 1104 8 0.4099540367404799 1104 9 0.40410233153505004 1104 10-11 0.3166444994722539 1104 12-13 0.284811310830996 1104 14-15 0.3440419112862827 1104 16-17 0.3609134066542765 1104 18-19 0.3483939653382251 1104 20-21 0.33847600382839005 1104 22-23 0.3385884277102207 1104 24-25 0.32713150214616604 1104 26-27 0.3299153568695914 1104 28-29 0.3040457747166485 1104 30-31 0.3261376389058981 1104 32-33 0.3568456176708992 1104 34-35 0.3717820708641071 1104 36-37 0.3662979812840561 1104 38-39 0.409668935370874 1104 40-41 0.36731160345743774 1104 42-43 0.4363011890687716 1104 44-45 0.38135979466476755 1104 46-47 0.3941831533202773 1104 48-49 0.2894626774513753 1104 50-51 0.30534675303012904 1104 52-53 0.3416887638525239 1104 54-55 0.3520845850452403 1104 56-57 0.40409092872776853 1104 58-59 0.3961105039198074 1104 60-61 0.35632972097546656 1104 62-63 0.34082895935259216 1104 64-65 0.3213783832208179 1104 66-67 0.35143712750006273 1104 68-69 0.2875730965634844 1104 70-71 0.27347924529474454 1104 72-73 0.2835846582954673 1104 74-75 0.2899760980315449 1104 76-77 0.19951368994547636 1104 78-79 0.32587922613391385 1104 80-81 0.33623112275474654 1104 82-83 0.33647345009994467 1104 84-85 0.2766033787642712 1104 86-87 0.30481544899793533 1104 88-89 0.3083814658502746 1104 90-91 0.28332676717720773 1104 92-93 0.3000837101829674 1104 94-95 0.2805749339032779 1104 96-97 0.2771758365187402 1104 98-99 0.291431355453458 1104 100 0.3194799890393689 1105 1 1.3490901091496568 1105 2 0.9946456442023823 1105 3 1.3481951190676753 1105 4 0.8382403849811766 1105 5 0.4340457741019108 1105 6 0.9935540912669438 1105 7 0.7251359430692688 1105 8 0.3664035062581519 1105 9 0.35011167721529546 1105 10-11 0.251492218342257 1105 12-13 0.24546805247615566 1105 14-15 0.30296378255795986 1105 16-17 0.2939788733479958 1105 18-19 0.30251076059838056 1105 20-21 0.2844466364318734 1105 22-23 0.2888317907712903 1105 24-25 0.29469694593415596 1105 26-27 0.3012653650442445 1105 28-29 0.3020101827276207 1105 30-31 0.3117790522528736 1105 32-33 0.31705027040572986 1105 34-35 0.3283987160425781 1105 36-37 0.3294388770491494 1105 38-39 0.3736198891767515 1105 40-41 0.3629356724251167 1105 42-43 0.35008570781715775 1105 44-45 0.32876341469501114 1105 46-47 0.33870999302345695 1105 48-49 0.31100547577033666 1105 50-51 0.32288740545355665 1105 52-53 0.3155171194912114 1105 54-55 0.3935773762398398 1105 56-57 0.3422050300867028 1105 58-59 0.29086640091759364 1105 60-61 0.28152664174047715 1105 62-63 0.23614274558735815 1105 64-65 0.23767586219596382 1105 66-67 0.23676240154136963 1105 68-69 0.20442987925660105 1105 70-71 0.20990582497485377 1105 72-73 0.21248308636545943 1105 74-75 0.2316990579350673 1105 76-77 0.17891185359360406 1105 78-79 0.24189566449152977 1105 80-81 0.24419562469092426 1105 82-83 0.24890704525947172 1105 84-85 0.24625742727223354 1105 86-87 0.2673703946067363 1105 88-89 0.25353189608324556 1105 90-91 0.22988633119636148 1105 92-93 0.22214235755018308 1105 94-95 0.2284241674038121 1105 96-97 0.24221901924341083 1105 98-99 0.242107080075467 1105 100 0.2453932616621799 1106 1 1.3381294545756504 1106 2 0.9851941668932405 1106 3 1.3233627358890487 1106 4 0.7904403847060166 1106 5 0.3876035529875068 1106 6 0.935621099150822 1106 7 0.6619652978368009 1106 8 0.31136664437010353 1106 9 0.3009309121848247 1106 10-11 0.2131912164955807 1106 12-13 0.19005208801997497 1106 14-15 0.2338648053731731 1106 16-17 0.22199755412579947 1106 18-19 0.2136840068814152 1106 20-21 0.22601574110796463 1106 22-23 0.2585495637056141 1106 24-25 0.25285456222588465 1106 26-27 0.21561507169067085 1106 28-29 0.22103488184558273 1106 30-31 0.24905443865478816 1106 32-33 0.2701442690132012 1106 34-35 0.274558762935726 1106 36-37 0.26924104977096164 1106 38-39 0.2755378560017263 1106 40-41 0.29511076809581027 1106 42-43 0.3094697117855816 1106 44-45 0.2943664553535683 1106 46-47 0.30499074606377263 1106 48-49 0.281331289061022 1106 50-51 0.28658624387627896 1106 52-53 0.2706430363109078 1106 54-55 0.32571073892427194 1106 56-57 0.3075312333496214 1106 58-59 0.24749637749813047 1106 60-61 0.2308312538221884 1106 62-63 0.2101784614711022 1106 64-65 0.18443727913301444 1106 66-67 0.18569742074746642 1106 68-69 0.19698672150079943 1106 70-71 0.2051486358329555 1106 72-73 0.15118490505788884 1106 74-75 0.19423530190896798 1106 76-77 0.06177045812601989 1106 78-79 0.16334258072880203 1106 80-81 0.2025153418081942 1106 82-83 0.20352209097804064 1106 84-85 0.2076398985913741 1106 86-87 0.22551522577311545 1106 88-89 0.24097062843570427 1106 90-91 0.21862560322560398 1106 92-93 0.20551031692887278 1106 94-95 0.18846516477045938 1106 96-97 0.22499719060080636 1106 98-99 0.2317064626339267 1106 100 0.23365591791882068 1107 1 1.274689944562546 1107 2 0.9007375638236788 1107 3 1.2638330523822958 1107 4 0.780726763421498 1107 5 0.36954611738990195 1107 6 0.9281852945146412 1107 7 0.6464382291600472 1107 8 0.2820905627295218 1107 9 0.2741809665529473 1107 10-11 0.1712020205853051 1107 12-13 0.13014900576801125 1107 14-15 0.17977912673201502 1107 16-17 0.19662393819676538 1107 18-19 0.13080364321132976 1107 20-21 0.14169190388101782 1107 22-23 0.16531646248517262 1107 24-25 0.17011884162428004 1107 26-27 0.1371422620986067 1107 28-29 0.1444803389514817 1107 30-31 0.15066910447935555 1107 32-33 0.18378843835032654 1107 34-35 0.16967291461424594 1107 36-37 0.15936457264925963 1107 38-39 0.1677016451474671 1107 40-41 0.1768415500862872 1107 42-43 0.16614492336118047 1107 44-45 0.14158903293331804 1107 46-47 0.20785910512957884 1107 48-49 0.19340705069670605 1107 50-51 0.15121883006751347 1107 52-53 0.16866199694973005 1107 54-55 0.18832879704727645 1107 56-57 0.15127271294433342 1107 58-59 0.13320901749971625 1107 60-61 0.1296986084207319 1107 62-63 0.05504478904956045 1107 64-65 0.07627463401148304 1107 66-67 0.09789116890089389 1107 68-69 0.04369404344428318 1107 70-71 0.08890963680218533 1107 72-73 0.11742376290791157 1107 74-75 0.11989505875637718 1107 76-77 -0.047062197490063795 1107 78-79 0.06671680758711318 1107 80-81 0.12287796643189353 1107 82-83 0.1529445353859984 1107 84-85 0.16257755483798775 1107 86-87 0.12512453351279618 1107 88-89 0.16612436683764997 1107 90-91 0.15546034292225386 1107 92-93 0.15357255330369668 1107 94-95 0.1188563348802738 1107 96-97 0.15133150279039498 1107 98-99 0.13649978794332185 1107 100 0.15426525749409237 1108 1 1.2442329563530379 1108 2 0.8817516696411509 1108 3 1.2247754497249659 1108 4 0.7371158479900686 1108 5 0.3408627476914603 1108 6 0.8807428333799621 1108 7 0.6118810271758832 1108 8 0.2746677218417801 1108 9 0.1989961056113998 1108 10-11 0.11290001924712811 1108 12-13 0.11641657361156632 1108 14-15 0.12449898574791973 1108 16-17 0.13544336223786502 1108 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-0.8397000546957045 2316 88-89 -0.700777255575133 2316 90-91 -0.7890117427585182 2316 92-93 -0.8582728641894874 2316 94-95 -0.8839786508444298 2316 96-97 -0.9811338026471397 2316 98-99 -1.1161788696209491 2316 100 -1.0286623390915786 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 70.0 7 1078.0 8 1325.0 9 505.0 10 465.0 11 843.0 12 1087.0 13 1493.0 14 2732.0 15 5474.0 16 10555.0 17 16801.0 18 24869.0 19 37269.0 20 56761.0 21 85727.0 22 135781.0 23 236161.0 24 442123.0 25 641870.0 26 645314.0 27 519701.0 28 425924.0 29 423457.0 30 480258.0 31 581542.0 32 719150.0 33 918410.0 34 1260955.0 35 1976862.0 36 3537116.0 37 7224118.0 38 9959380.0 39 3136209.0 40 7510.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 16.410612142171324 34.24949176034532 28.048444138298656 21.291451959184695 2 20.382964444122084 34.81143914932434 27.102828722306455 17.702767684247117 3 26.384926591749103 25.768671379880825 27.035641523176462 20.810760505193613 4 19.191443653202263 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19.905420277697466 30.03876611350193 26.127023634891405 23.928789973909197 26-27 19.95329127886624 26.236960872357574 33.83093126834822 19.978816580427964 28-29 19.951080344975715 26.328705543853626 33.81337874976945 19.906835361401214 30-31 19.921638844109772 26.254339254638893 33.81044759122008 20.013574310031252 32-33 20.053218290908823 26.374921597280967 33.6022480143212 19.96961209748901 34-35 20.0483214306046 26.478627959237087 33.51873326672945 19.954317343428862 36-37 19.991870436332132 26.435432772464644 33.575517136379105 19.99717965482412 38-39 20.001339316628684 26.42781102764491 33.58838279918846 19.982466856537943 40-41 20.005728601250116 26.45491628111738 33.59181955741483 19.94753556021767 42-43 20.009132721349097 26.481702054469913 33.53556853450751 19.973596689673478 44-45 20.019139694717214 26.539443545279568 33.50261471920179 19.938802040801434 46-47 20.0508066568864 26.595812528237982 33.43448147924097 19.91889933563464 48-49 20.062334976360695 26.604772116469373 33.35986137409919 19.97303153307075 50-51 20.068940562939684 26.672958391895442 33.28941750510034 19.96868354006454 52-53 20.156042169212046 26.811826231952402 33.021894509861006 20.01023708897455 54-55 20.486598883674784 27.078354925785685 32.21578948954863 20.2192567009909 56-57 20.9621007004755 27.679613569696127 30.89164105266049 20.466644677167885 58-59 21.025926515410706 28.31006313978784 30.122037075595575 20.541973269205876 60-61 20.98442029441205 28.632987028964596 29.790615754808513 20.591976921814837 62-63 20.936294146035014 28.807524833123864 29.617824976804275 20.638356044036847 64-65 20.94441284510066 28.894368319400808 29.562236654896868 20.59898218060166 66-67 20.86014070904207 28.73096451094419 29.788620940411732 20.620273839602007 68-69 20.807615041586132 28.553458349803197 30.063594244527224 20.575332364083447 70-71 20.730741869449222 28.44954562544287 30.276638370852226 20.543074134255683 72-73 20.686889501873967 28.377095658348107 30.34298230282115 20.593032536956777 74-75 20.74222001351247 28.46659761422032 30.208929796289812 20.582252575977407 76-77 20.78000101914442 28.592907238424004 30.01070907644828 20.616382665983295 78-79 20.88407460065533 28.81141510979982 29.63043249192326 20.67407779762159 80-81 20.9442858833828 29.04573768707932 29.268890618199688 20.741085811338188 82-83 20.939469998710546 29.276556776556777 28.93965565616478 20.844317568567895 84-85 20.949547879400995 29.3938191927133 28.721339463054964 20.935293464830742 86-87 20.95637374256923 29.481044974971798 28.567475398261564 20.995105884197404 88-89 20.986766661609003 29.571397044110853 28.385442559044975 21.05639373523516 90-91 21.055095723414315 29.578817872589784 28.204267465830764 21.161818938165137 92-93 21.04438352804433 29.67943101854562 28.09557741581469 21.18060803759535 94-95 21.06929975285409 29.76887642410369 27.978017614790794 21.183806208251426 96-97 21.054336811922703 29.74708025993583 27.90829935254887 21.290283575592593 98-99 21.049446931174113 29.763410338004952 27.845251913364056 21.34189081745688 100 21.038508004579466 29.783745135688726 27.74862303904422 21.429123820687586 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 55973.0 1 40280.5 2 19291.0 3 12708.5 4 11050.5 5 10881.0 6 11021.5 7 10868.5 8 10941.5 9 11212.5 10 12181.0 11 25222.0 12 196907.0 13 229249.0 14 99337.0 15 103430.5 16 117409.0 17 130778.5 18 142326.5 19 154435.0 20 169986.5 21 192409.5 22 223267.5 23 258026.5 24 293883.0 25 334067.5 26 382336.5 27 440718.5 28 506646.5 29 572440.5 30 629475.0 31 679943.5 32 734973.0 33 786414.5 34 828770.5 35 872442.0 36 922880.5 37 965716.5 38 1004008.0 39 1030594.0 40 1036241.0 41 1051045.0 42 1075836.5 43 1077128.0 44 1068440.0 45 1073453.0 46 1073779.5 47 1068496.5 48 1052500.5 49 1028952.5 50 1013578.5 51 994823.0 52 964718.5 53 913734.5 54 847972.0 55 786242.5 56 723713.0 57 653811.5 58 577453.5 59 495173.0 60 410925.0 61 334222.5 62 262825.0 63 196258.5 64 142874.0 65 103892.0 66 74606.5 67 54001.0 68 39438.0 69 28816.0 70 21284.5 71 16306.0 72 13268.0 73 10322.5 74 7877.5 75 6318.0 76 4760.5 77 2926.0 78 1582.0 79 959.5 80 607.5 81 397.5 82 258.5 83 158.5 84 112.5 85 95.5 86 69.0 87 40.0 88 22.5 89 16.5 90 9.5 91 7.5 92 7.0 93 7.0 94 5.5 95 3.0 96 2.5 97 1.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3324304097733532 2 0.0062710897838368475 3 2.4026388698076113 4 8.68167044289497E-4 5 4.475087857162356E-5 6 0.008729404713371369 7 0.007753835560509974 8 2.7447205523929117E-4 9 0.0 10-11 6.414292595266044E-5 12-13 1.491695952387452E-6 14-15 0.0 16-17 3.1325615000136486E-5 18-19 0.0 20-21 3.3413989333478923E-4 22-23 4.624257452401101E-5 24-25 5.608776780976819E-4 26-27 0.0034950436164438 28-29 0.006268106391932073 30-31 0.005686344970500967 32-33 0.015601647966020359 34-35 0.009937678434805204 36-37 0.005134417468117609 38-39 0.005376072212404377 40-41 0.008532500847656225 42-43 0.019184701643655017 44-45 0.034513369250388476 46-47 0.03364669390205136 48-49 0.040180322173508407 50-51 0.040310099721366115 52-53 0.06292271866360749 54-55 0.033640727118241814 56-57 0.02220986103509677 58-59 0.016817380167216133 60-61 0.018003278449364158 62-63 0.025093309311061716 64-65 0.045378882567578674 66-67 0.04816387891068605 68-69 0.0508444565371263 70-71 0.06144444797479153 72-73 0.06935043652244502 74-75 0.06027197495621499 76-77 0.06028838361169126 78-79 0.05484966016928661 80-81 0.05200201259617897 82-83 0.04873370676449806 84-85 0.050696778637839945 86-87 0.058431222150968874 88-89 0.0652393224776652 90-91 0.061338537562172016 92-93 0.06559285441838104 94-95 0.07373005583865458 96-97 0.07301851686936578 98-99 0.04790581551092302 100 0.006954286530030301 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3.3518895E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.077584345440265 #Duplication Level Percentage of deduplicated Percentage of total 1 66.39094112524751 26.60788542720266 2 17.298023432683905 13.865259862655826 3 6.54607725294402 7.870528897098958 4 3.020040835220838 4.84143765200948 5 1.626534256060607 3.2593781919008453 6 1.0444800125447147 2.5116141479932383 7 0.7174760876008035 2.0128295891660386 8 0.5142239837128686 1.6487084063760635 9 0.39867641539557913 1.4380188898098691 >10 2.148607983583103 15.233126788244364 >50 0.16467654364610976 4.705022681816714 >100 0.1293626231391227 8.467185575108193 >500 2.725858164559372E-4 0.06995507879347715 >1k 5.249004970503348E-4 0.4809978290783978 >5k 3.725541261589462E-5 0.10760224452091871 >10k+ 4.4706494690050955E-5 6.880448738224813 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 2138942 6.381302247583043 No Hit TAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 46308 0.13815491232631624 No Hit AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 42623 0.12716111315722073 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 40040 0.11945501186718716 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 5.041932319069587E-4 2.0883743333424327E-5 0.0 0.0 0.0 2 5.101600157165086E-4 2.386713523819923E-5 0.0 2.983391904774904E-6 0.0 3 5.280603671451579E-4 2.9833919047749037E-5 0.0 2.983391904774904E-6 2.983391904774904E-6 4 5.459607185738074E-4 3.580070285729885E-5 0.0 2.983391904774904E-6 2.983391904774904E-6 5 5.549108942881321E-4 5.668444619072317E-5 0.0 2.983391904774904E-6 2.983391904774904E-6 6 5.847448133358812E-4 7.16014057145977E-5 0.0 5.966783809549808E-6 2.983391904774904E-6 7 5.907115971454309E-4 8.05515814289224E-5 0.0 5.966783809549808E-6 5.966783809549808E-6 8 6.056285566693055E-4 8.353497333369731E-5 0.0 8.950175714324712E-6 5.966783809549808E-6 9 6.414292595266043E-4 8.651836523847221E-5 0.0 1.1933567619099615E-5 5.966783809549808E-6 10-11 6.608213069076412E-4 9.696023690518437E-5 0.0 1.3425263571487067E-5 1.1933567619099615E-5 12-13 6.861801380982279E-4 1.0591041261950908E-4 0.0 1.4916959523874519E-5 1.7900351428649424E-5 14-15 7.264559288126891E-4 1.267941559529334E-4 0.0 1.4916959523874519E-5 2.5358831190586682E-5 16-17 7.622566316699879E-4 1.3276093976248321E-4 0.0 1.7900351428649424E-5 2.9833919047749037E-5 18-19 8.338580373845856E-4 1.491695952387452E-4 0.0 1.9392047381036875E-5 3.132561500013649E-5 20-21 8.786089159562092E-4 1.5961146690545737E-4 0.0 2.0883743333424327E-5 3.2817310952523944E-5 22-23 9.651272811946813E-4 1.6557825071500718E-4 0.0 2.5358831190586682E-5 3.4309006904911396E-5 24-25 0.0011515892752431128 1.6706994666739462E-4 0.0 2.6850527142974134E-5 3.580070285729885E-5 26-27 0.0017139586492931824 1.8497029809604404E-4 0.0 2.6850527142974134E-5 3.580070285729885E-5 28-29 0.0030878106214420253 1.954121697627562E-4 0.0 2.983391904774904E-5 3.580070285729885E-5 30-31 0.006245730952646262 2.1182082523901817E-4 0.0 3.2817310952523944E-5 3.580070285729885E-5 32-33 0.012354225877672877 2.2673778476289268E-4 0.0 3.2817310952523944E-5 3.580070285729885E-5 34-35 0.02267676186819404 2.5209661595347935E-4 0.0 3.4309006904911396E-5 4.02757907144612E-5 36-37 0.03855288188945369 2.8043883904884097E-4 0.0 3.580070285729885E-5 4.1767486666848654E-5 38-39 0.060986497317408586 2.908807107155531E-4 0.0 3.72923988096863E-5 4.624257452401101E-5 40-41 0.09017152862586908 2.953557985727155E-4 0.0 3.878409476207375E-5 4.922596642878591E-5 42-43 0.12623029488293097 3.177312378585273E-4 0.0 3.878409476207375E-5 5.519275023833572E-5 44-45 0.17067686748026745 3.3563158928717664E-4 0.0 4.1767486666848654E-5 5.9667838095498075E-5 46-47 0.2251178029585999 3.669572042873132E-4 0.0 4.475087857162356E-5 5.9667838095498075E-5 48-49 0.29093590346579146 3.8336585976357515E-4 0.0 4.475087857162356E-5 5.9667838095498075E-5 50-51 0.36637693456183446 4.072329950017744E-4 0.0 4.624257452401101E-5 6.712631785743534E-5 52-53 0.45062643025672533 4.2960843428758614E-4 0.0 4.773427047639846E-5 6.861801380982279E-5 54-55 0.5458980673438071 4.6839252904965986E-4 0.0 4.773427047639846E-5 6.861801380982279E-5 56-57 0.6538043691476106 4.833094885735344E-4 0.0 4.773427047639846E-5 6.861801380982279E-5 58-59 0.7747376517036137 4.952430561926341E-4 0.0 5.0717662381173364E-5 7.010970976221024E-5 60-61 0.9101851358763468 5.235852792879955E-4 0.0 5.370105428594827E-5 7.309310166698515E-5 62-63 1.0556374248017424 5.489441104785822E-4 0.0 5.370105428594827E-5 7.45847976193726E-5 64-65 1.212613064959331 5.698278538120066E-4 0.0 5.370105428594827E-5 7.45847976193726E-5 66-67 1.3836747899953146 6.011534688121431E-4 0.0 5.370105428594827E-5 8.502666928608476E-5 68-69 1.5709005323713685 6.175621242884051E-4 0.0 5.9667838095498075E-5 8.651836523847221E-5 70-71 1.773293839191298 6.309873878598922E-4 0.0 5.9667838095498075E-5 8.651836523847221E-5 72-73 1.9887081599796175 6.563462190504789E-4 0.0 5.9667838095498075E-5 8.950175714324712E-5 74-75 2.2193482213539557 6.802133542886781E-4 0.0 6.265123000027298E-5 9.546854095279692E-5 76-77 2.4660821903586023 6.921469219077777E-4 0.0 6.563462190504789E-5 1.0740210857189654E-4 78-79 2.730746344710946 7.055721854792648E-4 0.0 7.16014057145977E-5 1.148605883338338E-4 80-81 3.011951915479314 7.339144085746264E-4 0.0 7.16014057145977E-5 1.1635228428622125E-4 82-83 3.3090858156272756 7.503230640508883E-4 0.0 7.16014057145977E-5 1.178439802386087E-4 84-85 3.623569631397455 7.62256631669988E-4 0.0 7.45847976193726E-5 1.1933567619099615E-4 86-87 3.957857202631531 7.697151114319252E-4 0.0 7.45847976193726E-5 1.1933567619099615E-4 88 4.220282321359341 7.816486790510249E-4 0.0 7.75681895241475E-5 1.1933567619099615E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 275885 0.0 86.68961 8 AGTGGTA 277160 0.0 85.60354 5 TGGTATC 279635 0.0 85.455315 7 GTGGTAT 277630 0.0 85.402824 6 AAGCAGT 270610 0.0 85.29818 1 GTATCAA 280155 0.0 85.22733 9 GCAGTGG 279740 0.0 84.77292 3 AGCAGTG 286575 0.0 82.47899 2 CAGTGGT 290360 0.0 81.5868 4 CAACGCA 271215 0.0 45.0498 12-13 ATCAACG 272290 0.0 44.93745 10-11 ACGCAGA 274410 0.0 44.56995 14-15 TCAACGC 270565 0.0 44.022594 12-13 AGAGTAC 277955 0.0 43.993793 18-19 AACGCAG 273200 0.0 43.684097 14-15 GAGTACT 273245 0.0 43.371014 20-21 CGCAGAG 275880 0.0 43.260937 16-17 AGTACTT 285355 0.0 42.64655 20-21 TATCAAC 279675 0.0 42.64401 10-11 CAGAGTA 287110 0.0 41.604324 18-19 >>END_MODULE