FastQCFastQC Report
Thu 26 May 2016
SRR1660726_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660726_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26829357
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT12936694.821841239057649No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1166430.4347588352564692Illumina Single End PCR Primer 1 (100% over 50bp)
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT336800.1255341303930616No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA305930.11402807752716548No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1666300.083.894898
AGTGGTA1689600.082.941145
TGGTATC1682500.082.791787
GTATCAA1689050.082.612029
GTGGTAT1699450.082.403626
AAGCAGT1741750.080.850171
GCAGTGG1780150.079.141583
CAGTGGT1781850.078.505844
AGCAGTG1860100.075.8213962
CAACGCA1642100.044.01642212-13
ATCAACG1658300.043.68322410-11
ACGCAGA1668550.043.36561214-15
AGAGTAC1661200.043.07641618-19
TCAACGC1638850.042.72134812-13
GAGTACT1613550.042.699220-21
AACGCAG1657000.042.37148314-15
AGTACTT1687850.042.12037320-21
CGCAGAG1686500.041.55172716-17
TATCAAC1694350.041.15603610-11
GTACTTT1708550.040.35248622-23
Produced by FastQC (version 0.11.5)