FastQCFastQC Report
Thu 26 May 2016
SRR1660726_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660726_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26829357
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT14210435.29659730570509No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACCGTAGATCTCGTA1126040.41970443048635114TruSeq Adapter, Index 10 (97% over 37bp)
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT353800.13187047307917218No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA284910.10619337615880992No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1898850.083.693878
AGTGGTA1918600.082.747235
TGGTATC1922300.082.706317
GTATCAA1921000.082.650469
AAGCAGT1898050.082.373681
GTGGTAT1929050.082.2963946
GCAGTGG1973550.080.487223
CAGTGGT2005750.079.0004044
AGCAGTG2053200.077.196872
CAACGCA1866150.043.81822212-13
ATCAACG1883150.043.5346510-11
ACGCAGA1899650.043.2515414-15
AGAGTAC1909800.042.9289418-19
TCAACGC1862150.042.58350412-13
GAGTACT1851200.042.54440720-21
AGTACTT1926750.042.19215420-21
AACGCAG1885200.042.1697814-15
CGCAGAG1918250.041.4997316-17
TATCAAC1924850.041.23656510-11
GTACTTT1943000.040.5065622-23
Produced by FastQC (version 0.11.5)