FastQCFastQC Report
Thu 26 May 2016
SRR1660725_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660725_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25159694
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT14038085.579590912353703No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA393030.15621414155513974No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT285410.11343937648844218No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGGTA1838850.082.8297045
GGTATCA1842100.082.44248
AAGCAGT1865300.081.865341
GTGGTAT1862800.081.715186
TGGTATC1860650.081.385887
GTATCAA1868300.081.1786049
GCAGTGG1888250.081.09833
CAGTGGT1927350.078.946344
AGCAGTG1963300.078.0740052
GAGTACT1727150.043.1728820-21
CAACGCA1840200.042.36733612-13
AGTACTT1804300.042.36643220-21
ATCAACG1848250.042.2800810-11
ACGCAGA1848850.042.2551114-15
AGAGTAC1828600.042.1875918-19
TCAACGC1831900.041.4123412-13
AACGCAG1843000.041.3154114-15
GTACTTT1820600.040.9473122-23
CGCAGAG1859000.040.87549216-17
TATCAAC1884900.040.13144710-11
Produced by FastQC (version 0.11.5)