FastQCFastQC Report
Thu 26 May 2016
SRR1660725_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660725_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25159694
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT11631314.6229934275035305No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT296890.11800223007481729No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA269760.10721911005753887No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGGTA1625650.079.8110355
AAGCAGT1593050.079.782421
GGTATCA1642150.079.277848
GCAGTGG1647050.078.852973
GTGGTAT1649250.078.5834966
TGGTATC1659750.078.443587
GTATCAA1671550.077.855099
AGCAGTG1703450.075.95912
CAGTGGT1717400.075.4661944
GAGTACT1519450.042.41296420-21
AGTACTT1608050.041.35951620-21
CAACGCA1637950.041.03173412-13
AGAGTAC1636550.041.02073318-19
ATCAACG1644800.040.93788510-11
ACGCAGA1647600.040.90828714-15
AACGCAG1634750.039.83548714-15
TCAACGC1630900.039.80426412-13
GTACTTT1624100.039.7248322-23
CGCAGAG1657200.039.3119116-17
TATCAAC1690800.038.45102310-11
Produced by FastQC (version 0.11.5)