##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660725_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 25159694 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.54704306022164 34.0 31.0 34.0 28.0 34.0 2 32.1086646363823 34.0 31.0 34.0 30.0 34.0 3 31.94378540533919 34.0 31.0 34.0 30.0 34.0 4 35.74523084422251 37.0 35.0 37.0 35.0 37.0 5 35.992973324715315 37.0 35.0 37.0 35.0 37.0 6 35.569988649305515 37.0 35.0 37.0 35.0 37.0 7 35.726944850760106 37.0 35.0 37.0 35.0 37.0 8 36.01707083559919 37.0 35.0 37.0 35.0 37.0 9 37.82953389655693 39.0 38.0 39.0 35.0 39.0 10-11 37.82975945573901 39.0 38.0 39.0 35.0 39.0 12-13 37.891934059293405 39.0 38.0 39.0 35.0 39.0 14-15 39.35202359774328 41.0 39.0 41.0 36.0 41.0 16-17 39.322480193916505 41.0 39.0 41.0 36.0 41.0 18-19 39.322170432597474 41.0 39.0 41.0 36.0 41.0 20-21 39.265830120986365 40.5 39.0 41.0 36.0 41.0 22-23 39.15379716064909 40.5 39.0 41.0 35.5 41.0 24-25 39.16192679052456 40.0 39.0 41.0 36.0 41.0 26-27 39.11018951581843 40.0 39.0 41.0 36.0 41.0 28-29 39.03444835219379 40.0 39.0 41.0 35.0 41.0 30-31 38.93639763663262 40.0 38.5 41.0 35.0 41.0 32-33 38.81428311489003 40.0 38.0 41.0 35.0 41.0 34-35 38.72120292877966 40.0 38.0 41.0 35.0 41.0 36-37 38.589595902875445 40.0 38.0 41.0 35.0 41.0 38-39 38.012151320282356 40.0 37.0 41.0 33.5 41.0 40-41 37.901454306240765 40.0 37.0 41.0 33.0 41.0 42-43 37.92232367770451 40.0 37.0 41.0 33.0 41.0 44-45 37.82076805067661 40.0 37.0 41.0 33.0 41.0 46-47 37.66899279458646 40.0 36.0 41.0 33.0 41.0 48-49 37.65940962954478 40.0 36.0 41.0 33.0 41.0 50-51 37.60885688832305 40.0 36.0 41.0 33.0 41.0 52-53 37.35417278922391 40.0 36.0 41.0 32.5 41.0 54-55 36.86974426239047 40.0 35.5 41.0 31.0 41.0 56-57 36.42315091352065 39.0 35.0 41.0 29.5 41.0 58-59 36.28849949446921 39.0 35.0 41.0 29.0 41.0 60-61 36.14493699724647 39.0 35.0 41.0 29.0 41.0 62-63 35.91562695476344 39.0 35.0 41.0 29.0 41.0 64-65 35.656625573427085 38.0 35.0 40.5 29.0 41.0 66-67 35.38012386001197 37.0 35.0 40.0 28.5 41.0 68-69 35.1089438726878 37.0 35.0 40.0 28.0 41.0 70-71 34.64009588113433 36.0 34.5 39.0 27.0 41.0 72-73 34.27094288189674 36.0 34.0 39.0 26.0 41.0 74-75 34.04293102690359 35.0 34.0 38.5 26.5 40.0 76-77 32.39394733894618 34.5 32.0 36.5 25.0 39.0 78-79 33.31487465626569 35.0 33.5 37.0 26.0 39.0 80-81 33.218187172705676 35.0 34.0 36.5 26.0 39.0 82-83 32.969259721521254 35.0 34.0 36.0 26.0 38.0 84-85 32.73230689928105 35.0 34.0 36.0 26.0 37.0 86-87 32.53046523538799 35.0 34.0 35.0 25.0 37.0 88-89 32.367170284344475 35.0 34.0 35.0 25.0 36.0 90-91 32.217387262341106 35.0 33.0 35.0 25.0 36.0 92-93 32.086003132629514 35.0 33.0 35.0 25.0 36.0 94-95 31.983679253014763 35.0 33.0 35.0 24.0 36.0 96-97 31.88442840362049 35.0 33.0 35.0 24.0 35.0 98-99 31.799907145134597 35.0 33.0 35.0 24.0 35.0 100 31.747326219468327 35.0 33.0 35.0 23.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.2486213903458356 1101 2 0.9579490420354446 1101 3 1.2368702582042168 1101 4 0.7661068988249369 1101 5 0.4863639910615021 1101 6 0.9189751781023787 1101 7 0.6927635969530783 1101 8 0.4240457025982636 1101 9 0.4671389619030606 1101 10-11 0.3898360314117255 1101 12-13 0.36807542074824084 1101 14-15 0.4558507954252917 1101 16-17 0.4878842004892405 1101 18-19 0.44507282965411576 1101 20-21 0.43167830528604867 1101 22-23 0.43854617409482444 1101 24-25 0.43176680179532667 1101 26-27 0.450987969733049 1101 28-29 0.4310085907428345 1101 30-31 0.4274942174988041 1101 32-33 0.4895566907336715 1101 34-35 0.49100936873937684 1101 36-37 0.4692405585875008 1101 38-39 0.6864248486493949 1101 40-41 0.646005787643837 1101 42-43 0.47253146041021665 1101 44-45 0.48189263570621677 1101 46-47 0.46983744542344397 1101 48-49 0.41916053476705173 1101 50-51 0.4161783601062865 1101 52-53 0.43231827737798056 1101 54-55 0.4486723970346276 1101 56-57 0.42176430423626243 1101 58-59 0.4002526951738403 1101 60-61 0.43320880913739757 1101 62-63 0.39064998757270075 1101 64-65 0.3968701469851226 1101 66-67 0.40462807980519955 1101 68-69 0.38954202756145406 1101 70-71 0.580112858612182 1101 72-73 0.586462846112795 1101 74-75 0.433453786412322 1101 76-77 0.3200323661150719 1101 78-79 0.3866807672005095 1101 80-81 0.4027488497063274 1101 82-83 0.3735675716686231 1101 84-85 0.40157589460054055 1101 86-87 0.35761639467556705 1101 88-89 0.3177088651436506 1101 90-91 0.34508337820622614 1101 92-93 0.3340415933826293 1101 94-95 0.2782591736815334 1101 96-97 0.3026280378940278 1101 98-99 0.2993512682257382 1101 100 0.3083797968227522 1102 1 1.1691372736970145 1102 2 0.8751332815206041 1102 3 1.1688667383269724 1102 4 0.7090308110039416 1102 5 0.4022991372799041 1102 6 0.8375367491272954 1102 7 0.6274680041447098 1102 8 0.3722824823527162 1102 9 0.38860050193142115 1102 10-11 0.327053500465631 1102 12-13 0.3117467684761124 1102 14-15 0.4136594206058817 1102 16-17 0.4195192240679049 1102 18-19 0.37995560820002083 1102 20-21 0.3694680881549388 1102 22-23 0.3656495477540105 1102 24-25 0.3778228142319975 1102 26-27 0.38538062597046974 1102 28-29 0.398932737813503 1102 30-31 0.39063858447332933 1102 32-33 0.39229214070413576 1102 34-35 0.3734950716098666 1102 36-37 0.3816116485014902 1102 38-39 0.5965157029145871 1102 40-41 0.5918206880780019 1102 42-43 0.41973936440141557 1102 44-45 0.3502383578695927 1102 46-47 0.37819744419903145 1102 48-49 0.3543903265917763 1102 50-51 0.3033864037751499 1102 52-53 0.33436337561652607 1102 54-55 0.33918997160575515 1102 56-57 0.35724908667434363 1102 58-59 0.34598676201533607 1102 60-61 0.3548854413446776 1102 62-63 0.31443560054883335 1102 64-65 0.30040330846387064 1102 66-67 0.3203354279775752 1102 68-69 0.2393062409129385 1102 70-71 0.39612588484637 1102 72-73 0.4160311979970004 1102 74-75 0.2774814139235744 1102 76-77 0.054289005793620504 1102 78-79 0.252414367230557 1102 80-81 0.29639427133868423 1102 82-83 0.3195435026231408 1102 84-85 0.28467859099448845 1102 86-87 0.24451084369071197 1102 88-89 0.2582058332581241 1102 90-91 0.2639383306288323 1102 92-93 0.24392546866112497 1102 94-95 0.21943220685707843 1102 96-97 0.22573295312831831 1102 98-99 0.2277793028276669 1102 100 0.19577597442334493 1103 1 1.1686335743370684 1103 2 0.8730957591201403 1103 3 1.1343860794918008 1103 4 0.7123548885991511 1103 5 0.41948512143734007 1103 6 0.8363085854325263 1103 7 0.6298448037551267 1103 8 0.36349474581565744 1103 9 0.35226545776980345 1103 10-11 0.3173152170079163 1103 12-13 0.2873048562206151 1103 14-15 0.3735038507768067 1103 16-17 0.39522266978399045 1103 18-19 0.33857716460236276 1103 20-21 0.36442404238532333 1103 22-23 0.32589175417966487 1103 24-25 0.3393864224957568 1103 26-27 0.3810214627269062 1103 28-29 0.37692499339564023 1103 30-31 0.3783079028397154 1103 32-33 0.3643481642167927 1103 34-35 0.3610970200830437 1103 36-37 0.29738052340638177 1103 38-39 0.5667315274306048 1103 40-41 0.5824134945833848 1103 42-43 0.418678133654943 1103 44-45 0.36736507419668385 1103 46-47 0.40333775022722307 1103 48-49 0.34187805184408404 1103 50-51 0.34473275962614025 1103 52-53 0.3525811104688543 1103 54-55 0.32225044711091044 1103 56-57 0.3506576022671837 1103 58-59 0.3657976612097258 1103 60-61 0.3614595503461686 1103 62-63 0.3306052512499349 1103 64-65 0.30278695899720276 1103 66-67 0.293362995201214 1103 68-69 0.2546123910249847 1103 70-71 0.4510724002640245 1103 72-73 0.43714860460771376 1103 74-75 0.29812241945080586 1103 76-77 0.1302475722551435 1103 78-79 0.3161225220031483 1103 80-81 0.33493776917288187 1103 82-83 0.3084014763467309 1103 84-85 0.28913031729822336 1103 86-87 0.24598507839609596 1103 88-89 0.23660737132332343 1103 90-91 0.255039798461965 1103 92-93 0.30117823323851667 1103 94-95 0.2452251053526595 1103 96-97 0.2623700405750391 1103 98-99 0.27324095717711927 1103 100 0.287856235125556 1104 1 1.0780713068519212 1104 2 0.8064921878674696 1104 3 1.0985646385836425 1104 4 0.6605172177259746 1104 5 0.38841184740351054 1104 6 0.7851452425120229 1104 7 0.598187688142275 1104 8 0.33802027084547603 1104 9 0.33877327680517766 1104 10-11 0.26378619298451866 1104 12-13 0.2519330638028592 1104 14-15 0.3061154664406516 1104 16-17 0.31889234718894244 1104 18-19 0.28190743746102953 1104 20-21 0.2607969586593626 1104 22-23 0.27703438565644944 1104 24-25 0.24949377702574083 1104 26-27 0.2590919813367947 1104 28-29 0.2328925940656248 1104 30-31 0.25734044489417585 1104 32-33 0.2839996722273881 1104 34-35 0.2969503796182664 1104 36-37 0.29466492462577776 1104 38-39 0.5491616604551695 1104 40-41 0.4860654019821098 1104 42-43 0.302949632078068 1104 44-45 0.2355692195083421 1104 46-47 0.27546994888869847 1104 48-49 0.2305899559180773 1104 50-51 0.1838136126501837 1104 52-53 0.23118163898720212 1104 54-55 0.22273810200567112 1104 56-57 0.2566980625313562 1104 58-59 0.24838409813792595 1104 60-61 0.23754843293473016 1104 62-63 0.24049059741489032 1104 64-65 0.1823323290680463 1104 66-67 0.16788555405241112 1104 68-69 0.1211566926786034 1104 70-71 0.33042606296779553 1104 72-73 0.37719975694713526 1104 74-75 0.2134569113351361 1104 76-77 0.05583105340346606 1104 78-79 0.19730055396892254 1104 80-81 0.22471023575351978 1104 82-83 0.21439876273650071 1104 84-85 0.1547093797504573 1104 86-87 0.1516371599401154 1104 88-89 0.17987642661239533 1104 90-91 0.18395708473897798 1104 92-93 0.1996371031090547 1104 94-95 0.20144972782364334 1104 96-97 0.20001244036861365 1104 98-99 0.19495934486493027 1104 100 0.22231841985140477 1105 1 1.059150103602473 1105 2 0.7921920416121111 1105 3 1.0575949062968597 1105 4 0.6652724565427803 1105 5 0.34261355353313405 1105 6 0.7953389751795612 1105 7 0.5897618312649158 1105 8 0.33266772175341686 1105 9 0.3128317522224222 1105 10-11 0.24134384234043438 1105 12-13 0.22566142572782155 1105 14-15 0.3064092406330303 1105 16-17 0.2966337608203773 1105 18-19 0.26411843498157594 1105 20-21 0.28106094638647505 1105 22-23 0.2738356263515058 1105 24-25 0.26091357387461755 1105 26-27 0.279615177256062 1105 28-29 0.30622419514192956 1105 30-31 0.30241427084740025 1105 32-33 0.319929155856876 1105 34-35 0.31942624258945074 1105 36-37 0.32697244780778334 1105 38-39 0.5636544310427567 1105 40-41 0.5492253920052477 1105 42-43 0.3654597910514852 1105 44-45 0.3174144534953669 1105 46-47 0.3246496510104251 1105 48-49 0.28286179298550707 1105 50-51 0.2988885150984544 1105 52-53 0.3041591258297274 1105 54-55 0.3349459451325387 1105 56-57 0.3662012785626132 1105 58-59 0.36077000466251974 1105 60-61 0.3162467225683301 1105 62-63 0.27844731778630916 1105 64-65 0.26285029655998926 1105 66-67 0.28089177589563974 1105 68-69 0.30206845509038516 1105 70-71 0.4378438804203739 1105 72-73 0.4450797912639288 1105 74-75 0.2982243048466984 1105 76-77 0.2002806751099655 1105 78-79 0.30394015697212495 1105 80-81 0.3069803162696161 1105 82-83 0.32642360906678647 1105 84-85 0.3203821206909083 1105 86-87 0.30150326155087015 1105 88-89 0.2925668888441706 1105 90-91 0.3134431316956636 1105 92-93 0.3032243843669775 1105 94-95 0.28748939345460656 1105 96-97 0.3068096185785869 1105 98-99 0.2820582593552139 1105 100 0.2756634262266324 1106 1 1.004532170621811 1106 2 0.7453680000382903 1106 3 1.0202691414440856 1106 4 0.6017086591046876 1106 5 0.3251408790508208 1106 6 0.7547090633757989 1106 7 0.5407861823603639 1106 8 0.2531636547247018 1106 9 0.24702576477589844 1106 10-11 0.16709544663120113 1106 12-13 0.16383073919909918 1106 14-15 0.21593770387737266 1106 16-17 0.18960429170093818 1106 18-19 0.1656740298603765 1106 20-21 0.200234283255476 1106 22-23 0.19162645194894168 1106 24-25 0.20241871854963023 1106 26-27 0.1529696963577294 1106 28-29 0.16380833595683697 1106 30-31 0.19000450305640726 1106 32-33 0.22415466232308034 1106 34-35 0.20260578307098598 1106 36-37 0.21998640599590402 1106 38-39 0.45976404539436544 1106 40-41 0.4251541605741451 1106 42-43 0.2645788591056757 1106 44-45 0.2610263172654541 1106 46-47 0.2671743198128027 1106 48-49 0.22457591200066673 1106 50-51 0.23127600574706264 1106 52-53 0.24203290475657724 1106 54-55 0.25010902236645194 1106 56-57 0.28937935657552316 1106 58-59 0.30067690853803697 1106 60-61 0.30115832640447593 1106 62-63 0.27111473389967955 1106 64-65 0.20584380261045965 1106 66-67 0.2104402056709418 1106 68-69 0.20368537813923382 1106 70-71 0.3700703411884376 1106 72-73 0.32912899990989786 1106 74-75 0.21945745534748795 1106 76-77 0.08263936142561334 1106 78-79 0.23513263838393073 1106 80-81 0.28239521557272695 1106 82-83 0.2958736672865214 1106 84-85 0.264159802796577 1106 86-87 0.2933872710140477 1106 88-89 0.27963315880251827 1106 90-91 0.24855379490293217 1106 92-93 0.2813497221049701 1106 94-95 0.2550375064301704 1106 96-97 0.2779081762634057 1106 98-99 0.26910236548511435 1106 100 0.24746942743515987 1107 1 0.973002583131386 1107 2 0.6825318205152087 1107 3 0.9704193294762469 1107 4 0.5985214375569328 1107 5 0.29427197966797536 1107 6 0.7034194542294969 1107 7 0.5035370753277917 1107 8 0.2324077738745629 1107 9 0.2089004118471962 1107 10-11 0.13471780619372709 1107 12-13 0.09527608102293783 1107 14-15 0.13016285105715752 1107 16-17 0.1200463703159187 1107 18-19 0.08612789807049381 1107 20-21 0.09481827206045068 1107 22-23 0.118017183588087 1107 24-25 0.10851006770474925 1107 26-27 0.08519825551898919 1107 28-29 0.11253136171337275 1107 30-31 0.10131553105536995 1107 32-33 0.09769406659452073 1107 34-35 0.13802080895513313 1107 36-37 0.14695902346910117 1107 38-39 0.3536533020480306 1107 40-41 0.32255422065627926 1107 42-43 0.17033299145449377 1107 44-45 0.14041102278673634 1107 46-47 0.14725128146752553 1107 48-49 0.14386191950733718 1107 50-51 0.11784067509329077 1107 52-53 0.09116845335953627 1107 54-55 0.10757859470307096 1107 56-57 0.08974643031106666 1107 58-59 0.10894669811805358 1107 60-61 0.13310317863063403 1107 62-63 0.09630247567081085 1107 64-65 0.07972043811160745 1107 66-67 0.10956249724153366 1107 68-69 0.1004869222012843 1107 70-71 0.26301850912463465 1107 72-73 0.24430520718784976 1107 74-75 0.10235673120985922 1107 76-77 -0.06209949628724587 1107 78-79 0.05262186126375923 1107 80-81 0.11619952837084213 1107 82-83 0.14179931113338284 1107 84-85 0.15440438999554829 1107 86-87 0.12403442785225849 1107 88-89 0.1391038055097411 1107 90-91 0.13820676046943703 1107 92-93 0.15914871607053982 1107 94-95 0.10273510164661559 1107 96-97 0.12305572308163804 1107 98-99 0.12774727706070266 1107 100 0.15028635594335782 1108 1 0.9166018541375145 1108 2 0.648596737251502 1108 3 0.9200719237576926 1108 4 0.5549732977271375 1108 5 0.24897744816191647 1108 6 0.6549979686325074 1108 7 0.4786436565823067 1108 8 0.2213607616707236 1108 9 0.16680569616377738 1108 10-11 0.0920925242603019 1108 12-13 0.095785816058509 1108 14-15 0.10015420065159475 1108 16-17 0.1053211502357172 1108 18-19 0.07764162334812852 1108 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-0.6962311714415286 2316 78-79 -0.9277751404249557 2316 80-81 -1.0951978821909663 2316 82-83 -1.1796014645823263 2316 84-85 -0.9719675558985728 2316 86-87 -0.8041420233797822 2316 88-89 -0.7198963053850136 2316 90-91 -0.7567567373878923 2316 92-93 -0.8336189056469863 2316 94-95 -0.8133826672142845 2316 96-97 -0.880181202899891 2316 98-99 -1.0463809624433082 2316 100 -1.0089340953962918 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 40.0 7 668.0 8 888.0 9 395.0 10 306.0 11 540.0 12 730.0 13 1182.0 14 2829.0 15 7794.0 16 16791.0 17 24510.0 18 31089.0 19 41406.0 20 58024.0 21 83959.0 22 117907.0 23 188014.0 24 305415.0 25 378571.0 26 377261.0 27 328828.0 28 302416.0 29 319861.0 30 369229.0 31 443076.0 32 543356.0 33 695760.0 34 960995.0 35 1520856.0 36 2816206.0 37 5629177.0 38 7390386.0 39 2196319.0 40 4910.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 16.178726665313413 32.25229964391736 26.932259786133557 24.636713904635677 2 20.988345887075937 31.9467655066876 29.10174294969891 17.963145656537552 3 24.473499394609043 26.32070113092976 26.944583056974462 22.26121641748674 4 19.07550243253585 28.123348166801254 25.910577801738636 26.890571598924257 5 21.875509247254456 31.95963431019245 26.35131267944383 19.81354376310927 6 27.683928354299553 27.125041234639536 26.06633403891819 19.124696372142726 7 20.99038151614093 26.560502943721197 31.822238884832544 20.626876655305328 8 25.62950555666028 26.555302270578178 26.865020693669265 20.950171479092276 9 25.27033616825134 25.121010021863142 28.424516149700235 21.184137660185282 10-11 20.71473128264611 29.383528116882413 29.09909484339814 20.80264575707334 12-13 20.718006902191973 26.790095195335983 28.79469473558864 23.697203166883405 14-15 20.659104598013 32.28261639430114 26.13694943984613 20.921329567839734 16-17 23.551869376432812 26.621567800536784 26.149878695825564 23.676684127204844 18-19 20.745564314096985 29.40834654030371 26.220330819603767 23.625758325995537 20-21 23.469136400870433 29.509743793733378 26.240484732079032 20.780635073317157 22-23 23.497970552744167 26.805069325536696 28.898158393866986 20.798801727852158 24-25 20.770487958642747 29.431338568697125 26.041113965974382 23.75705950668575 26-27 20.864365870032923 26.648587789253476 31.660559543866 20.826486796847597 28-29 20.793108368127637 26.81736975605436 31.590995845591223 20.79852603022678 30-31 20.817625622074655 26.665858724165247 31.6538059656524 20.862709688107696 32-33 20.893826745317792 26.81044367367455 31.452410325454505 20.84331925555315 34-35 20.93275714407347 26.879921007106134 31.337944723576943 20.849377125243453 36-37 20.82794743419412 26.83408261432851 31.423505638184558 20.914464313292815 38-39 20.83452673850106 26.856081295466538 31.467842766293046 20.841549199739354 40-41 20.84394697392072 26.866307946830663 31.374300182404667 20.915444896843947 42-43 20.83837828975267 26.9151556278081 31.36732399491419 20.879142087525036 44-45 20.880665585081964 26.952671376062586 31.35174532009868 20.814917718756767 46-47 20.896029964896464 27.00836202902573 31.27540945094962 20.82019855512818 48-49 20.96336925499631 27.01198579757892 31.17612050174763 20.84852444567714 50-51 20.915662443659876 27.08971801043432 31.1630768942052 20.831542651700605 52-53 21.077354570689444 27.128693531257248 30.876509391933304 20.917442506120004 54-55 21.349343472303453 27.27502674084985 30.2838715625755 21.0917582242712 56-57 21.624913988441254 27.750739894208365 29.408113257444384 21.216232859905997 58-59 21.72909913342179 28.19740080403533 28.81404997996302 21.25945008257986 60-61 21.81259882436818 28.461334552254996 28.384368124732006 21.341698498644813 62-63 21.847317045759056 28.64236826642071 28.12788069695824 21.382433990861998 64-65 21.80542307671892 28.74992840526574 28.117461367719958 21.327187150295384 66-67 21.757765646237143 28.696041885564515 28.18090170416317 21.365290764035173 68-69 21.620907145292552 28.694280565134278 28.28873816962577 21.396074119947393 70-71 21.736042525887946 28.59028724304168 28.257425305890372 21.416244925180003 72-73 21.582344575925276 28.51584384658579 28.34012830648484 21.561683271004103 74-75 21.678784581430534 28.523327137529837 28.25767567516731 21.540212605872316 76-77 21.74664837419559 28.61625693458848 28.10027300713757 21.536821684078355 78-79 21.82360120416283 28.785143958109 27.7967859206629 21.59446891706527 80-81 21.881854271959856 28.939115474781367 27.50266048843235 21.676369764826422 82-83 21.870868246669687 29.126858352053674 27.15421591236152 21.84805748891512 84-85 21.811294681217447 29.173769101219587 26.979216732641913 22.03571948492105 86-87 21.791536563824724 29.18650992249285 26.873642974617535 22.148310539064894 88-89 21.77476360015657 29.21260140618055 26.712487373377357 22.300147620285518 90-91 21.771350910335734 29.1775171335064 26.59682669139786 22.45430526476001 92-93 21.750829763381038 29.18548738836012 26.508929838268912 22.554753009989927 94-95 21.760480420251596 29.172418897227708 26.38635152675341 22.680749155767288 96-97 21.757592660434874 29.08494286127425 26.314688197491492 22.842776280799388 98-99 21.723739266755743 29.060277219049397 26.317531626977647 22.89845188721722 100 21.70216311169589 28.99993536922655 26.21792240761635 23.079979111461217 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 25746.0 1 18929.5 2 9931.5 3 7290.0 4 6684.5 5 6801.0 6 7181.5 7 7216.0 8 7273.5 9 7567.5 10 8237.5 11 13787.0 12 100168.5 13 117484.0 14 51704.0 15 53373.0 16 60120.5 17 66741.0 18 73215.0 19 80148.0 20 89137.5 21 103233.0 22 124293.0 23 148986.5 24 175020.5 25 203532.0 26 235454.5 27 275999.5 28 326643.5 29 378221.5 30 420186.5 31 459661.0 32 503455.0 33 541624.0 34 571549.0 35 606847.5 36 647815.0 37 682667.0 38 720012.5 39 746766.0 40 755239.0 41 775820.0 42 807298.5 43 814529.0 44 808623.5 45 816846.5 46 827852.0 47 832197.0 48 827608.5 49 820329.0 50 816593.5 51 810961.5 52 798924.0 53 767846.0 54 723474.5 55 680469.5 56 634826.5 57 579213.5 58 514550.5 59 445996.5 60 371755.0 61 300907.0 62 237565.5 63 179609.0 64 133044.0 65 98643.0 66 72716.0 67 53974.0 68 40518.0 69 30943.5 70 24149.0 71 18906.5 72 15203.0 73 11973.5 74 9282.0 75 7575.0 76 5763.5 77 3726.0 78 2228.5 79 1373.5 80 925.0 81 621.5 82 406.0 83 278.5 84 200.0 85 138.0 86 81.5 87 51.0 88 33.5 89 20.5 90 11.5 91 8.5 92 9.5 93 10.0 94 6.5 95 4.0 96 2.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.29613237744465415 2 0.004745685698721138 3 1.854394572525405 4 5.84267837279738E-4 5 3.974611137957401E-5 6 0.007317259104979576 7 0.006244114097731078 8 2.662989462431459E-4 9 3.9746111379574014E-6 10-11 5.7631861500382314E-5 12-13 2.1860361258765707E-5 14-15 0.0 16-17 2.980958353468051E-5 18-19 0.0 20-21 2.52387807260295E-4 22-23 5.325978924862918E-4 24-25 3.676515302610596E-4 26-27 0.0027166467127938835 28-29 0.004930505116636156 30-31 0.004523107474995522 32-33 0.012039097136872968 34-35 0.007901526942259313 36-37 0.00405211605514757 38-39 0.6129486312512386 40-41 0.00644085734905997 42-43 0.015081661962979359 44-45 0.026973698487747902 46-47 0.025948248814154893 48-49 0.03150276787945036 50-51 0.03440820862129722 52-53 0.05191239607286161 54-55 0.030336219510459866 56-57 0.024237178719264233 58-59 0.019688236271871988 60-61 0.020747470140137635 62-63 0.02278843295947876 64-65 0.037562062559266425 66-67 0.0379555490619242 68-69 0.03942416787739946 70-71 0.1505065999610329 72-73 0.05426934047767036 74-75 0.05101214665011426 76-77 0.053518138972596406 78-79 0.049406403750379475 80-81 0.04791791187921443 82-83 0.045596738974647305 84-85 0.0462128037010307 86-87 0.052627826077693944 88-89 0.053343256082526276 90-91 0.04756218418236724 92-93 0.05073988578716418 94-95 0.057139009719275595 96-97 0.05654281804858199 98-99 0.03708312191714255 100 0.005564455593140362 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2.5159694E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 31.172872637740028 #Duplication Level Percentage of deduplicated Percentage of total 1 52.82641314572093 16.467510489001942 2 20.16461946398696 12.571782286787181 3 10.018450109107944 9.369116078363241 4 5.352765359609687 6.674442912592779 5 2.996537717217386 4.670534430650091 6 1.8955560011047219 3.5453955480084773 7 1.2181481062069697 2.658122303808585 8 0.923104551977212 2.302065650408296 9 0.6575581475592839 1.844817874719644 >10 3.4954891574819333 19.428345489202943 >50 0.2647357168552054 5.830857248119135 >100 0.18467393370137272 8.938108869758752 >500 9.864879508291544E-4 0.1981454058698212 >1k 8.853345337397118E-4 0.49436050103320056 >5k 1.2794497652970466E-5 0.022865568621708958 >10k+ 6.397248826433053E-5 4.983529343054168 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 1163131 4.6229934275035305 No Hit AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 29689 0.11800223007481729 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 26976 0.10721911005753887 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.08719501914450947 7.949222275914803E-6 0.0 0.0 2.782227796570181E-5 2 0.09495346008580231 7.949222275914803E-6 0.0 3.9746111379574014E-6 2.782227796570181E-5 3 0.10328424503096102 1.1923833413872203E-5 0.0 7.949222275914803E-6 2.782227796570181E-5 4 0.10551002726821718 1.1923833413872203E-5 0.0 1.1923833413872203E-5 3.179688910365921E-5 5 0.10589556454859904 1.5898444551829606E-5 0.0 1.5898444551829606E-5 5.564455593140362E-5 6 0.10607044743866917 1.5898444551829606E-5 0.0 1.5898444551829606E-5 5.564455593140362E-5 7 0.10626520338442907 1.5898444551829606E-5 0.0 1.9873055689787005E-5 5.564455593140362E-5 8 0.10675408055439783 1.5898444551829606E-5 0.0 1.9873055689787005E-5 6.359377820731842E-5 9 0.10719923700184907 1.5898444551829606E-5 0.0 2.3847666827744407E-5 6.359377820731842E-5 10-11 0.1074019421698849 2.980958353468051E-5 0.0 3.3784194672637914E-5 6.359377820731842E-5 12-13 0.10756688853211013 3.7758805810595306E-5 0.0 3.577150024161661E-5 6.756838934527581E-5 14-15 0.10779940328368064 4.7695333655488814E-5 0.0 4.372072251753141E-5 6.756838934527581E-5 16-17 0.10805974031321684 6.160647263833971E-5 0.0 7.154300048323322E-5 6.955569491425451E-5 18-19 0.10848502370497828 7.949222275914802E-5 0.0 8.346683389710542E-5 7.353030605221193E-5 20-21 0.10878709415146305 9.539066731097763E-5 0.0 8.346683389710542E-5 7.949222275914802E-5 22-23 0.10906531693112007 1.2520025084565814E-4 0.0 8.545413946608413E-5 8.147952832812671E-5 24-25 0.109538295656537 1.3911138982850902E-4 0.0 8.744144503506283E-5 8.346683389710542E-5 26-27 0.11046040544054311 1.4706061210442383E-4 0.0 9.141605617302024E-5 8.346683389710542E-5 28-29 0.11315121718094029 1.5898444551829606E-4 0.0 9.539066731097763E-5 8.346683389710542E-5 30-31 0.11906543855422089 1.8084480677706176E-4 0.0 9.539066731097763E-5 8.346683389710542E-5 32-33 0.1307090618828671 1.8283211234604044E-4 0.0 9.539066731097763E-5 8.346683389710542E-5 34-35 0.1509497691029152 1.9078133462195525E-4 0.0 9.936527844893503E-5 8.346683389710542E-5 36-37 0.18453920782979316 2.0667977917378488E-4 0.0 9.936527844893503E-5 8.346683389710542E-5 38-39 0.23224646531869586 2.2257822372561447E-4 0.0 9.936527844893503E-5 8.346683389710542E-5 40-41 0.2980938480412361 2.2257822372561447E-4 0.0 9.936527844893503E-5 9.340336174199892E-5 42-43 0.3816203011054109 2.623243351051885E-4 0.0 9.936527844893503E-5 9.539066731097763E-5 44-45 0.48721578251309416 2.643116406741672E-4 0.0 9.936527844893503E-5 9.539066731097763E-5 46-47 0.618976129041951 2.8219739079497546E-4 0.0 9.936527844893503E-5 9.539066731097763E-5 48-49 0.7803711762154182 3.100196687606773E-4 0.0 1.0135258401791373E-4 9.539066731097763E-5 50-51 0.9705861287502145 3.179688910365921E-4 0.0 1.1128911186280724E-4 9.936527844893503E-5 52-53 1.182901906517623 3.2393080774352815E-4 0.0 1.1128911186280724E-4 1.0333988958689243E-4 54-55 1.4262792703281686 3.457911690022939E-4 0.0 1.1526372300076464E-4 1.0333988958689243E-4 56-57 1.706433710998234 3.736134469679957E-4 0.0 1.3513677869055163E-4 1.1128911186280724E-4 58-59 2.0281884191437305 3.795753636749318E-4 0.0 1.3513677869055163E-4 1.1128911186280724E-4 60-61 2.38677187409354 4.054103360716549E-4 0.0 1.3513677869055163E-4 1.1923833413872203E-4 62-63 2.7718103407775945 4.1932147505450583E-4 0.0 1.3513677869055163E-4 1.2321294527667943E-4 64-65 3.1843054212026587 4.5708028086510115E-4 0.0 1.3513677869055163E-4 1.2321294527667943E-4 66-67 3.633603413459639 4.8092794769284556E-4 0.0 1.3513677869055163E-4 1.2321294527667943E-4 68-69 4.124318841079704 4.888771699687604E-4 0.0 1.3712408425953034E-4 1.2520025084565814E-4 70-71 4.6503904220774706 5.0477561452059E-4 0.0 1.3911138982850905E-4 1.2718755641463685E-4 72-73 5.20787931681522 5.544582537450575E-4 0.0 1.4308600096646644E-4 1.3911138982850905E-4 74-75 5.795944895037277 5.922170595556528E-4 0.0 1.4308600096646644E-4 1.3911138982850905E-4 76-77 6.41760189929178 6.021535874005463E-4 0.0 1.5103522324238123E-4 1.4904791767340254E-4 78-79 7.076033993100234 6.081155041074824E-4 0.0 1.5500983438033864E-4 1.5500983438033864E-4 80-81 7.768337723026361 6.180520319523759E-4 0.0 1.5500983438033864E-4 1.5699713994931735E-4 82-83 8.48793908224798 6.577981433319498E-4 0.0 1.5500983438033864E-4 1.5898444551829604E-4 84-85 9.23332175661596 6.677346711768434E-4 0.0 1.5500983438033864E-4 1.5898444551829604E-4 86-87 10.007260024704593 6.677346711768434E-4 0.0 1.5500983438033864E-4 1.5898444551829604E-4 88 10.605939801970564 6.677346711768434E-4 0.0 1.5500983438033864E-4 1.6295905665625346E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGTGGTA 162565 0.0 79.811035 5 AAGCAGT 159305 0.0 79.78242 1 GGTATCA 164215 0.0 79.27784 8 GCAGTGG 164705 0.0 78.85297 3 GTGGTAT 164925 0.0 78.583496 6 TGGTATC 165975 0.0 78.44358 7 GTATCAA 167155 0.0 77.85509 9 AGCAGTG 170345 0.0 75.9591 2 CAGTGGT 171740 0.0 75.466194 4 GAGTACT 151945 0.0 42.412964 20-21 AGTACTT 160805 0.0 41.359516 20-21 CAACGCA 163795 0.0 41.031734 12-13 AGAGTAC 163655 0.0 41.020733 18-19 ATCAACG 164480 0.0 40.937885 10-11 ACGCAGA 164760 0.0 40.908287 14-15 AACGCAG 163475 0.0 39.835487 14-15 TCAACGC 163090 0.0 39.804264 12-13 GTACTTT 162410 0.0 39.72483 22-23 CGCAGAG 165720 0.0 39.31191 16-17 TATCAAC 169080 0.0 38.451023 10-11 >>END_MODULE