FastQCFastQC Report
Thu 26 May 2016
SRR1660724_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660724_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24041961
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT12928785.377589623408839No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT383170.1593755184945188No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1647050.083.908668
AGTGGTA1664550.083.1046145
GTGGTAT1666550.082.991396
TGGTATC1666900.082.5883567
GTATCAA1675400.082.378629
AAGCAGT1734000.080.200871
GCAGTGG1738600.080.190233
CAGTGGT1767550.078.207394
AGCAGTG1817950.076.717452
CAACGCA1620350.044.2336112-13
ATCAACG1634200.044.01489610-11
ACGCAGA1642150.043.69476714-15
AGAGTAC1643100.043.08419418-19
TCAACGC1621150.042.76782612-13
GAGTACT1597250.042.6195920-21
AACGCAG1632600.042.55383314-15
CGCAGAG1650250.042.02494416-17
AGTACTT1688600.041.7006320-21
TATCAAC1679900.041.05210510-11
GTACTTT1696550.040.1460622-23
Produced by FastQC (version 0.11.5)