FastQCFastQC Report
Thu 26 May 2016
SRR1660724_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660724_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24041961
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT13561555.640783628257279No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT402570.16744474379606555No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1744950.083.837748
AGTGGTA1747700.083.493835
GTGGTAT1756400.083.037476
TGGTATC1764350.082.919527
GTATCAA1767600.082.697029
AAGCAGT1755550.081.764381
GCAGTGG1789400.081.461473
CAGTGGT1853450.078.592054
AGCAGTG1861300.078.184842
CAACGCA1707500.044.20648212-13
ATCAACG1722150.043.94631210-11
ACGCAGA1733800.043.64695714-15
AGAGTAC1748350.043.19885318-19
TCAACGC1706450.042.73115512-13
GAGTACT1704250.042.565620-21
AACGCAG1721200.042.42635314-15
CGCAGAG1740000.041.9898916-17
AGTACTT1796200.041.8285220-21
TATCAAC1771200.041.21807510-11
GTACTTT1798150.040.3544822-23
Produced by FastQC (version 0.11.5)