FastQCFastQC Report
Thu 26 May 2016
SRR1660723_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660723_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37532645
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT19428695.176477703609751No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT569220.15165997493648528No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA426280.11357579515112778No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA2506850.084.565868
GTGGTAT2536950.083.6309746
AGTGGTA2543100.083.46725
TGGTATC2543100.083.081127
GTATCAA2551050.082.990989
AAGCAGT2593350.082.3126751
GCAGTGG2647850.080.6550753
CAGTGGT2679850.079.096374
AGCAGTG2726650.078.374472
CAACGCA2474350.044.48942612-13
ATCAACG2499600.044.16444410-11
ACGCAGA2508850.043.9083314-15
AGAGTAC2504350.043.3542418-19
TCAACGC2473200.043.00189612-13
AACGCAG2492250.042.76333214-15
GAGTACT2444800.042.55703720-21
CGCAGAG2530200.042.05106416-17
AGTACTT2561550.041.9863420-21
TATCAAC2557500.041.37544610-11
GTACTTT2559600.040.62774722-23
Produced by FastQC (version 0.11.5)