##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660723_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 37532645 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.840676403168494 34.0 31.0 34.0 30.0 34.0 2 31.963603018119294 34.0 31.0 34.0 30.0 34.0 3 32.02351257152274 34.0 31.0 34.0 30.0 34.0 4 35.42501675541386 37.0 35.0 37.0 33.0 37.0 5 35.39248643414287 37.0 35.0 37.0 33.0 37.0 6 35.463853693231584 37.0 35.0 37.0 33.0 37.0 7 35.40855055645559 37.0 35.0 37.0 33.0 37.0 8 35.42252660317438 37.0 35.0 37.0 33.0 37.0 9 37.101955564282775 39.0 37.0 39.0 33.0 39.0 10-11 37.03319506259151 39.0 37.0 39.0 33.0 39.0 12-13 37.063661007637485 39.0 37.0 39.0 33.0 39.0 14-15 38.43375255061294 40.0 38.0 41.0 33.0 41.0 16-17 38.412769683564804 40.0 38.0 41.0 33.0 41.0 18-19 38.377298549036446 40.0 38.0 41.0 33.5 41.0 20-21 38.259580160151245 40.0 38.0 41.0 33.5 41.0 22-23 38.089148100273775 40.0 38.0 41.0 33.0 41.0 24-25 38.07114839628276 40.0 38.0 41.0 33.0 41.0 26-27 38.04905193865234 40.0 38.0 41.0 33.0 41.0 28-29 37.96361292949112 40.0 38.0 41.0 33.0 41.0 30-31 37.869365361807034 40.0 38.0 41.0 32.0 41.0 32-33 37.74090668270249 40.0 38.0 41.0 32.0 41.0 34-35 37.58203569186237 40.0 37.5 41.0 31.5 41.0 36-37 37.39267742787645 40.0 37.0 41.0 31.0 41.0 38-39 36.98359501442012 40.0 35.0 41.0 30.5 41.0 40-41 36.920043138446545 39.5 35.0 41.0 30.0 41.0 42-43 36.92913229270146 40.0 35.0 41.0 30.0 41.0 44-45 36.93201397077131 40.0 35.0 41.0 30.0 41.0 46-47 37.02288133436905 40.0 35.0 41.0 31.0 41.0 48-49 36.91626273607949 40.0 35.0 41.0 30.0 41.0 50-51 35.54968169709329 38.5 34.0 40.0 28.0 40.5 52-53 35.715492992833305 38.5 35.0 39.5 28.0 40.5 54-55 36.12820286180204 39.0 35.0 41.0 27.5 41.0 56-57 35.78317166296167 39.0 35.0 41.0 25.5 41.0 58-59 35.60159983395788 39.0 35.0 41.0 25.0 41.0 60-61 35.38182147834239 39.0 35.0 41.0 25.0 41.0 62-63 35.15730994977839 38.5 35.0 41.0 25.0 41.0 64-65 34.906128358393076 38.0 34.0 40.5 24.0 41.0 66-67 34.63901262487629 37.0 34.0 40.0 24.0 41.0 68-69 34.360871249548225 37.0 34.0 40.0 23.5 41.0 70-71 34.05848948295544 36.0 34.0 39.0 24.0 41.0 72-73 33.71667873393948 36.0 33.5 39.0 23.5 41.0 74-75 33.337630028472546 35.0 33.0 38.5 23.0 40.5 76-77 32.94811881763196 35.0 33.0 37.0 22.0 39.5 78-79 32.581324377751685 35.0 33.0 37.0 20.5 39.0 80-81 32.26133027661653 35.0 33.0 36.5 20.0 39.0 82-83 31.94906062442442 35.0 32.0 36.0 20.0 38.0 84-85 31.66046455026018 35.0 32.0 35.5 19.0 37.0 86-87 31.44241510290575 35.0 32.0 35.0 19.0 37.0 88-89 31.234295944237346 35.0 32.0 35.0 18.5 36.0 90-91 31.0582549671093 35.0 31.0 35.0 18.0 36.0 92-93 30.90389432985605 35.0 31.0 35.0 18.0 36.0 94-95 30.72240023318367 35.0 31.0 35.0 17.0 35.5 96-97 30.584923751043924 35.0 31.0 35.0 16.5 35.0 98-99 30.47920251823446 34.5 31.0 35.0 15.5 35.0 100 30.394721421844903 34.0 31.0 35.0 15.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5159311058908074 1101 2 0.5348481629401185 1101 3 0.5285593271395115 1101 4 0.458115818022101 1101 5 0.45927751022366436 1101 6 0.4323344029447185 1101 7 0.4654039579443179 1101 8 0.4625466782292804 1101 9 0.5720207375678612 1101 10-11 0.5812195574361496 1101 12-13 0.5567518295510183 1101 14-15 0.6919657326322195 1101 16-17 0.6567552240232217 1101 18-19 0.6440917389460807 1101 20-21 0.6857233218521017 1101 22-23 0.7349846004849852 1101 24-25 0.7185419434653184 1101 26-27 0.7011410498291113 1101 28-29 0.7017392457956504 1101 30-31 0.656237975423501 1101 32-33 0.6551501139897908 1101 34-35 0.66019449744892 1101 36-37 0.6362934303546055 1101 38-39 0.6572239618502351 1101 40-41 0.6201289157699534 1101 42-43 0.677055661408545 1101 44-45 0.6754162772019896 1101 46-47 0.7057853061131709 1101 48-49 0.743777557951951 1101 50-51 0.6780690517630603 1101 52-53 0.7200342187610786 1101 54-55 0.8026962609247832 1101 56-57 0.8004306188115464 1101 58-59 0.7165979249436916 1101 60-61 0.7076060988830335 1101 62-63 0.6594490597004423 1101 64-65 0.6629785587979669 1101 66-67 0.6561720936927671 1101 68-69 0.6445770592628577 1101 70-71 0.5969342012056487 1101 72-73 0.5664288448527373 1101 74-75 0.528606804017663 1101 76-77 0.5289957342381655 1101 78-79 0.5363007020718342 1101 80-81 0.47690010225658597 1101 82-83 0.4965975700411498 1101 84-85 0.4406215008127212 1101 86-87 0.42472236098577554 1101 88-89 0.3934460369924011 1101 90-91 0.38711458283487943 1101 92-93 0.4172748209866981 1101 94-95 0.4583007540778894 1101 96-97 0.38850970388881123 1101 98-99 0.33381648006995945 1101 100 0.30981021773550665 1102 1 0.4390352025459663 1102 2 0.48664817889559586 1102 3 0.4701622089471442 1102 4 0.4120510992323929 1102 5 0.4108964726968338 1102 6 0.3848331957555118 1102 7 0.3894366630061228 1102 8 0.423159832904922 1102 9 0.4907247007247477 1102 10-11 0.4846801212389309 1102 12-13 0.48499422878634846 1102 14-15 0.5874128997432493 1102 16-17 0.5788742728476564 1102 18-19 0.5484507146783812 1102 20-21 0.5540768363577868 1102 22-23 0.6194873560798513 1102 24-25 0.5543930504809182 1102 26-27 0.5746091482616436 1102 28-29 0.5854663238229136 1102 30-31 0.564231420842539 1102 32-33 0.5425086687499885 1102 34-35 0.5365620395933703 1102 36-37 0.5454365699363848 1102 38-39 0.5646942930404677 1102 40-41 0.5432778278637542 1102 42-43 0.5448381789746861 1102 44-45 0.5828147694119252 1102 46-47 0.5888832298617928 1102 48-49 0.6190233202835174 1102 50-51 0.552893897104525 1102 52-53 0.622822115185933 1102 54-55 0.6242252715567176 1102 56-57 0.6199090829816356 1102 58-59 0.5387056702107529 1102 60-61 0.5389910358070296 1102 62-63 0.5582316938411296 1102 64-65 0.5217250662268995 1102 66-67 0.5047104628129659 1102 68-69 0.49816155203068035 1102 70-71 0.5369697246754228 1102 72-73 0.4712940170413802 1102 74-75 0.46050525456335833 1102 76-77 0.4201350564913966 1102 78-79 0.4685724740586963 1102 80-81 0.4375193186748092 1102 82-83 0.41343485571844596 1102 84-85 0.392506747194485 1102 86-87 0.3528948545271895 1102 88-89 0.33863901289100795 1102 90-91 0.30820382046330863 1102 92-93 0.32669699890924875 1102 94-95 0.3660078658312784 1102 96-97 0.35012166741029915 1102 98-99 0.30627758261353577 1102 100 0.2569543684685307 1103 1 0.4162851713912872 1103 2 0.43268293709042993 1103 3 0.4517375934785406 1103 4 0.3922264025515574 1103 5 0.40531207800611924 1103 6 0.36490548821254976 1103 7 0.3748288737569965 1103 8 0.3987852022801661 1103 9 0.4599550817782472 1103 10-11 0.4400023922143461 1103 12-13 0.4603238925624211 1103 14-15 0.5577459670734086 1103 16-17 0.5233280012676929 1103 18-19 0.5079654626280572 1103 20-21 0.5476731409903337 1103 22-23 0.5736678787750051 1103 24-25 0.5937939087823025 1103 26-27 0.5535253053658593 1103 28-29 0.5456838408409723 1103 30-31 0.5163507174441762 1103 32-33 0.4796910410183983 1103 34-35 0.5090394933612927 1103 36-37 0.523184890226915 1103 38-39 0.544144649802135 1103 40-41 0.5145331573614769 1103 42-43 0.530569838751056 1103 44-45 0.5490303072450047 1103 46-47 0.5645392844236241 1103 48-49 0.6337077691688222 1103 50-51 0.5683318771253667 1103 52-53 0.5535339191440087 1103 54-55 0.5967191883201295 1103 56-57 0.6322689052979769 1103 58-59 0.5418811610793242 1103 60-61 0.5563330837985632 1103 62-63 0.5154600784352752 1103 64-65 0.5169003801375993 1103 66-67 0.48693095400954434 1103 68-69 0.4837680900604795 1103 70-71 0.46268434438979256 1103 72-73 0.44412685139028696 1103 74-75 0.4783161444090709 1103 76-77 0.4628321333633565 1103 78-79 0.4532663791850169 1103 80-81 0.39196789206175 1103 82-83 0.3688602172021085 1103 84-85 0.3826983608121246 1103 86-87 0.346246422893973 1103 88-89 0.34747455529042526 1103 90-91 0.3036634519734207 1103 92-93 0.24000080554858627 1103 94-95 0.3022360485453035 1103 96-97 0.31666329421221207 1103 98-99 0.29906496664962745 1103 100 0.31382815944139253 1104 1 0.35921944206017287 1104 2 0.39968364730547634 1104 3 0.3683975036938918 1104 4 0.32912439190371146 1104 5 0.33889133293811824 1104 6 0.2696848815913171 1104 7 0.29426590391796026 1104 8 0.30799724722516686 1104 9 0.36562302988166806 1104 10-11 0.3391920802072903 1104 12-13 0.34241336782157816 1104 14-15 0.4326448461569825 1104 16-17 0.4090064646478808 1104 18-19 0.37181591279501447 1104 20-21 0.3257326780768963 1104 22-23 0.37247952490921676 1104 24-25 0.40615733738464854 1104 26-27 0.35896215202428294 1104 28-29 0.32386234908720013 1104 30-31 0.3444169048005463 1104 32-33 0.3159129117740207 1104 34-35 0.2859401228035452 1104 36-37 0.30748728040577333 1104 38-39 0.3003716844604938 1104 40-41 0.3081020087684081 1104 42-43 0.29379978332239176 1104 44-45 0.3347164183049074 1104 46-47 0.35283575997906524 1104 48-49 0.36231986090679413 1104 50-51 0.2905999552541374 1104 52-53 0.3113584520058552 1104 54-55 0.42112942618811644 1104 56-57 0.4321125939308388 1104 58-59 0.4207298991999906 1104 60-61 0.4465641490373997 1104 62-63 0.3889795343779312 1104 64-65 0.35630304856945116 1104 66-67 0.3949179968017589 1104 68-69 0.3438527581519608 1104 70-71 0.35734106888134676 1104 72-73 0.36983269428200316 1104 74-75 0.34717535481167516 1104 76-77 0.328757980339752 1104 78-79 0.3368649078017185 1104 80-81 0.30290140125254794 1104 82-83 0.30735979013485704 1104 84-85 0.2883560876397162 1104 86-87 0.21909669772218265 1104 88-89 0.2411276704287637 1104 90-91 0.22187837821460832 1104 92-93 0.24698568201171156 1104 94-95 0.30923699061899157 1104 96-97 0.2607192576469224 1104 98-99 0.2567510396930679 1104 100 0.18633567948571894 1105 1 0.27932976009760324 1105 2 0.31988508832430185 1105 3 0.3197426497549145 1105 4 0.27653853810871 1105 5 0.28925155347396014 1105 6 0.2475208793606285 1105 7 0.2791423821605292 1105 8 0.2764320300517582 1105 9 0.29813823830726705 1105 10-11 0.29430997398157643 1105 12-13 0.3032104216789051 1105 14-15 0.36162567889346064 1105 16-17 0.33930114270126666 1105 18-19 0.34898433221222547 1105 20-21 0.3564284737992196 1105 22-23 0.3885801356154417 1105 24-25 0.43005150305351236 1105 26-27 0.40612797101941567 1105 28-29 0.38260964273465703 1105 30-31 0.4088907880428465 1105 32-33 0.38401970881945147 1105 34-35 0.3966138417055518 1105 36-37 0.377863313056956 1105 38-39 0.41675261223824833 1105 40-41 0.39385813701671424 1105 42-43 0.44183774606766946 1105 44-45 0.4402588369639773 1105 46-47 0.459568688999731 1105 48-49 0.4657173015356122 1105 50-51 0.3623094816151067 1105 52-53 0.40717628873716194 1105 54-55 0.5228790785539346 1105 56-57 0.5554725055597629 1105 58-59 0.4844449387291334 1105 60-61 0.4517528909297326 1105 62-63 0.4639612699153517 1105 64-65 0.47646461758873215 1105 66-67 0.4588303061952601 1105 68-69 0.4675245552702947 1105 70-71 0.4374947967680711 1105 72-73 0.3993439140268933 1105 74-75 0.3916170807538464 1105 76-77 0.43009654276537646 1105 78-79 0.45458050819282647 1105 80-81 0.40941166557298203 1105 82-83 0.4083419193235507 1105 84-85 0.3837558350468093 1105 86-87 0.31584361867664157 1105 88-89 0.35404131181244836 1105 90-91 0.33793735710870365 1105 92-93 0.3206246976374807 1105 94-95 0.3727429427721347 1105 96-97 0.3623252489373847 1105 98-99 0.31712213917385057 1105 100 0.29416004540645346 1106 1 0.2516550833069502 1106 2 0.30437870756358265 1106 3 0.2839470874166281 1106 4 0.2807412658997279 1106 5 0.28520136414773845 1106 6 0.2557426880195308 1106 7 0.2635928897951132 1106 8 0.26823355577692354 1106 9 0.285753514725009 1106 10-11 0.2884086079213759 1106 12-13 0.29304831870091164 1106 14-15 0.3468862985768695 1106 16-17 0.31870536707911157 1106 18-19 0.29488445204911073 1106 20-21 0.32627907384708976 1106 22-23 0.3396499479577386 1106 24-25 0.34193686473616225 1106 26-27 0.3627745871415229 1106 28-29 0.36472613126343845 1106 30-31 0.3500417937046407 1106 32-33 0.33927626603980343 1106 34-35 0.37782124543350193 1106 36-37 0.3075719211735688 1106 38-39 0.30768906317906186 1106 40-41 0.3505817824488844 1106 42-43 0.3684471709819661 1106 44-45 0.3946347317273933 1106 46-47 0.42505905644437547 1106 48-49 0.42773621190287514 1106 50-51 0.30615692053040533 1106 52-53 0.35179367263742733 1106 54-55 0.4600308839390337 1106 56-57 0.4682250246395441 1106 58-59 0.37601969224507314 1106 60-61 0.3775587784669412 1106 62-63 0.35909561303313353 1106 64-65 0.34928482279997297 1106 66-67 0.3430683371611849 1106 68-69 0.3429269419019718 1106 70-71 0.32539204223448337 1106 72-73 0.3243708435782793 1106 74-75 0.3267104134256087 1106 76-77 0.34061184927003296 1106 78-79 0.3486933099801348 1106 80-81 0.3076772428477099 1106 82-83 0.3350497545323883 1106 84-85 0.31405688417288147 1106 86-87 0.3027928816080525 1106 88-89 0.3153988694895702 1106 90-91 0.3003898522551509 1106 92-93 0.2709016957782673 1106 94-95 0.3097046132112169 1106 96-97 0.33803471606335833 1106 98-99 0.30226858151180735 1106 100 0.26058443579984925 1107 1 0.20567597192954423 1107 2 0.2165827847025632 1107 3 0.2152975968962636 1107 4 0.2161991551146869 1107 5 0.21200659018077062 1107 6 0.17737944742168565 1107 7 0.20290283165984135 1107 8 0.18723196831405886 1107 9 0.2180219875540388 1107 10-11 0.20624041638657786 1107 12-13 0.19899790819359708 1107 14-15 0.2468163712480802 1107 16-17 0.23492438233137847 1107 18-19 0.23098029812663157 1107 20-21 0.2521471335791645 1107 22-23 0.2673729533767357 1107 24-25 0.2757981212176972 1107 26-27 0.2607684801217971 1107 28-29 0.27286882659934264 1107 30-31 0.23951071534782642 1107 32-33 0.195873541230128 1107 34-35 0.24120884420417354 1107 36-37 0.21869320373262013 1107 38-39 0.24663151687192908 1107 40-41 0.26018501295986596 1107 42-43 0.3043190415871919 1107 44-45 0.2835271261415855 1107 46-47 0.2782707536315172 1107 48-49 0.3277059782212106 1107 50-51 0.2555219919888714 1107 52-53 0.2480029930170886 1107 54-55 0.3477108673879883 1107 56-57 0.3995042058381415 1107 58-59 0.31682988415855107 1107 60-61 0.36248291194966953 1107 62-63 0.34402331965165445 1107 64-65 0.3345315573783907 1107 66-67 0.3206187565836487 1107 68-69 0.2945158764735325 1107 70-71 0.3093837429367383 1107 72-73 0.2711051460498126 1107 74-75 0.28891789388799083 1107 76-77 0.26308136865284126 1107 78-79 0.27148561442206187 1107 80-81 0.2234640714059637 1107 82-83 0.2764468393064128 1107 84-85 0.23975229422877575 1107 86-87 0.2311328083792894 1107 88-89 0.2381645982918208 1107 90-91 0.30408192747864504 1107 92-93 0.2597375143631595 1107 94-95 0.272486087867712 1107 96-97 0.3353203073968132 1107 98-99 0.3282276323850546 1107 100 0.3146593106127398 1108 1 0.13989606677264277 1108 2 0.17525064858438455 1108 3 0.19790195127104937 1108 4 0.18304631572397057 1108 5 0.1888442086059925 1108 6 0.1251148201110155 1108 7 0.14653855451388154 1108 8 0.1640440433959398 1108 9 0.18963923689166506 1108 10-11 0.1595810655846961 1108 12-13 0.16977647645670402 1108 14-15 0.22501338835873952 1108 16-17 0.19395869614690042 1108 18-19 0.1875521855700768 1108 20-21 0.1722068196390012 1108 22-23 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2316 88-89 -1.3283683262785715 2316 90-91 -1.1976368487117988 2316 92-93 -1.0865228022143114 2316 94-95 -1.3277011470329256 2316 96-97 -1.483776720291818 2316 98-99 -1.3585055059490863 2316 100 -1.1630228396043591 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 19512.0 3 4822.0 4 3546.0 5 2885.0 6 4675.0 7 24121.0 8 47870.0 9 56097.0 10 58406.0 11 56204.0 12 54861.0 13 57787.0 14 65491.0 15 79210.0 16 97870.0 17 119430.0 18 130744.0 19 145518.0 20 167392.0 21 203778.0 22 262872.0 23 380655.0 24 551702.0 25 663554.0 26 652646.0 27 562571.0 28 516212.0 29 518116.0 30 579502.0 31 701056.0 32 876726.0 33 1133912.0 34 1563983.0 35 2388650.0 36 4131091.0 37 7691320.0 38 9839781.0 39 3111672.0 40 6405.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 14.338148182955457 32.52992831884967 28.228254064382778 24.90366943381209 2 20.404749447326605 33.39752102972628 28.148775340370157 18.048954182576963 3 24.693282434342215 25.81212275892523 27.18983140385302 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26.901808163572827 29.380903835088333 20.385730449677258 24-25 20.40120871642765 29.711849761075342 26.284619233336926 23.602322289160078 26-27 20.448820770019243 26.755211306600007 32.405859246340626 20.39010867704013 28-29 20.398227111722296 26.87256573738384 32.36859140725924 20.36061574363462 30-31 20.34195911271754 26.775371721358805 32.399749451976206 20.48291971394745 32-33 20.500248164001132 26.910086004315804 32.181517997684445 20.40814783399862 34-35 20.506453372792947 27.041839737008207 32.05644705831207 20.39525983188678 36-37 20.44639333072514 26.9826186859361 32.131060901395344 20.439927081943416 38-39 20.48642610281826 26.98763268612529 32.14968068544851 20.37626052560794 40-41 20.49965166281515 27.026411550379116 32.13619260489284 20.33774418191289 42-43 20.505477968652972 27.05864639383588 32.07293542830745 20.362940209203703 44-45 20.538568519976693 27.13511900721449 32.02513077497608 20.30118169783274 46-47 20.53510792611779 27.234363507054137 31.964240450705507 20.266288116122563 48-49 20.632041667634475 27.237723160563814 31.846052255321705 20.284182916480006 50-51 20.682523350578972 27.292947705165727 31.774790953225928 20.249737991029374 52-53 20.82486677319868 27.40335265912977 31.516823332590626 20.254957235080926 54-55 21.17347630747816 27.637713447485297 30.78898817856394 20.3998220664726 56-57 21.66735418345117 28.15369896921559 29.72096118207153 20.457985665261706 58-59 21.79863779726254 28.635575227437204 29.097813739901124 20.467973235399135 60-61 21.94581541950103 28.864404578826946 28.758154132332763 20.43162586933926 62-63 22.022481203516904 28.98972267415743 28.593153862169636 20.394642260156033 64-65 22.14139291241589 29.0244368914584 28.531759263062824 20.30241093306289 66-67 22.236792521937613 28.87537649314783 28.675640986036576 20.21218999887798 68-69 22.40456465040527 28.730966889693505 28.797195052557484 20.067273407343738 70-71 22.54805715093052 28.610636903375948 28.86986636483365 19.971439580859883 72-73 22.718030490166583 28.52562398249535 28.834038080359335 19.922307446978728 74-75 22.94788048281936 28.584525969655868 28.690323317002903 19.777270230521868 76-77 23.257676475896346 28.683378468283927 28.37954644402687 19.679398611792852 78-79 23.511012035466276 28.814024707683554 28.059626002407047 19.615337254443123 80-81 23.765194231849705 28.932288607021654 27.776196718084595 19.526320443044042 82-83 23.967769603441784 29.029914163048343 27.482861369709568 19.519454863800302 84-85 24.10775629714152 29.050136502296013 27.31550357032987 19.526603630232593 86-87 24.294505474042573 29.051772389496882 27.211331936481187 19.44239019997936 88-89 24.501453173057993 29.05673243219786 27.050588426166875 19.39122596857727 90-91 24.684291728729523 29.000864856577586 26.941419711427113 19.373423703265782 92-93 24.83551847364281 28.99439040638116 26.88947805706523 19.2806130629108 94-95 24.964398417732355 28.960999928139863 26.798008464395824 19.27659318973196 96-97 25.07348932488797 28.95307926717391 26.71642774752126 19.257003660416856 98-99 25.21979401992735 28.98358212399327 26.62429091227273 19.17233294380665 100 25.28951362212979 28.973998918634486 26.5321529915863 19.204334467649424 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 53033.0 1 38633.0 2 20109.5 3 14949.5 4 13740.5 5 13364.5 6 13526.5 7 13688.0 8 13396.5 9 13589.5 10 14627.0 11 18569.5 12 153870.0 13 189442.0 14 90208.5 15 93289.0 16 105872.0 17 118054.0 18 129325.5 19 140600.0 20 155273.5 21 179918.5 22 214656.0 23 251322.0 24 287063.0 25 328550.5 26 376751.5 27 435567.5 28 511471.5 29 583273.5 30 639634.0 31 702313.5 32 773876.0 33 836519.0 34 887207.5 35 950870.5 36 1025439.0 37 1078224.0 38 1118793.0 39 1149502.0 40 1159506.5 41 1174049.5 42 1206652.5 43 1219589.5 44 1214831.0 45 1220044.0 46 1224047.0 47 1228763.5 48 1224037.0 49 1208534.0 50 1190643.5 51 1164086.0 52 1129358.0 53 1080783.5 54 1017143.5 55 945907.0 56 866559.0 57 780867.5 58 693627.0 59 598428.5 60 497861.5 61 407333.0 62 324673.0 63 247415.5 64 184683.0 65 136607.5 66 100691.5 67 76338.0 68 58516.5 69 44812.0 70 35385.5 71 29244.0 72 24833.5 73 19950.5 74 15630.0 75 12862.5 76 10160.5 77 7277.5 78 5222.0 79 4074.0 80 3281.5 81 2701.0 82 2167.5 83 1809.0 84 1556.5 85 1319.0 86 1134.5 87 992.0 88 845.5 89 714.5 90 613.0 91 527.0 92 465.0 93 413.5 94 358.0 95 289.0 96 246.0 97 202.0 98 162.5 99 122.0 100 73.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4056362134882847 2 0.38467579356584114 3 0.1689249452043681 4 0.175521869028948 5 0.275885166100071 6 0.10353919900928912 7 0.137459004021699 8 0.14289160809210222 9 0.1359776269431584 10-11 0.13260989200201584 12-13 0.1344149872730792 14-15 0.14079743114294238 16-17 0.14508703023727743 18-19 0.15670624865367203 20-21 0.13742836402816802 22-23 0.09969987460249603 24-25 0.09505858166937076 26-27 0.08940483677609186 28-29 0.09946674421693436 30-31 0.10346992598043649 32-33 0.10469286137441153 34-35 0.10513514302016286 36-37 0.10112263604123822 38-39 0.10050051095519648 40-41 0.10146766901186954 42-43 0.06391102998469732 44-45 0.06684047980098391 46-47 0.08542030544343465 48-49 0.09006559489745526 50-51 0.09929622599206638 52-53 0.10407739715652867 54-55 0.10655257576437792 56-57 0.10717869737131502 58-59 0.10654191837532367 60-61 0.10432384927840817 62-63 0.10868138922796408 64-65 0.11569927992018682 66-67 0.12408131641135337 68-69 0.1294646300573807 70-71 0.12762489827189105 72-73 0.11443771149089012 74-75 0.11217434849049408 76-77 0.11416994459090214 78-79 0.1127645014093731 80-81 0.10713340346783445 82-83 0.1102786654124696 84-85 0.11598436507738796 86-87 0.12949127353001633 88-89 0.13737907360379212 90-91 0.14434500952437537 92-93 0.14338451233586122 94-95 0.1335144378979952 96-97 0.13001881428820164 98-99 0.12517103444215028 100 0.11951728954887139 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3.7532645E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 39.23978191078266 #Duplication Level Percentage of deduplicated Percentage of total 1 70.38237988349614 27.617892369902453 2 15.082375492232202 11.836582500234499 3 5.328384859087911 6.272539794219781 4 2.5232962534696335 3.960543787297736 5 1.4842155088037434 2.912014643703011 6 0.9673447093887041 2.2775037257377306 7 0.6792817003475363 1.8658406044336062 8 0.5004269421796561 1.570931525872764 9 0.36981926966660106 1.3060464739310114 >10 2.2999819912662387 16.60459491087274 >50 0.214134767512758 5.903414175369636 >100 0.16640172059175118 11.54963086596833 >500 0.0014379989442656375 0.33983844516572764 >1k 4.4429663512811514E-4 0.3949412213738671 >5k 4.747678781153677E-5 0.11083393008737255 >10k+ 2.7129590142110287E-5 5.476851025829762 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 1942869 5.176477703609751 No Hit AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 56922 0.15165997493648528 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 42628 0.11357579515112778 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.001028438043734994 5.32869452712432E-6 0.0 0.0 1.8650430844935123E-5 2 0.0010364310855256803 7.993041790686481E-6 0.0 0.0 2.131477810849728E-5 3 0.0010390954327892425 7.993041790686481E-6 0.0 5.32869452712432E-6 3.1972167162745924E-5 4 0.0010417597800528046 1.8650430844935123E-5 0.0 7.993041790686481E-6 3.1972167162745924E-5 5 0.0010417597800528046 2.6643472635621604E-5 0.0 7.993041790686481E-6 3.1972167162745924E-5 6 0.001047088474579929 3.730086168987025E-5 0.0 7.993041790686481E-6 4.7958250744118886E-5 7 0.001047088474579929 3.9965208953432405E-5 0.0 7.993041790686481E-6 5.0622598007681044E-5 8 0.0010524171691070534 3.9965208953432405E-5 0.0 7.993041790686481E-6 5.8615639798367525E-5 9 0.0010524171691070534 4.529390348055673E-5 0.0 7.993041790686481E-6 5.8615639798367525E-5 10-11 0.001067071079056645 5.7283466166586446E-5 0.0 7.993041790686481E-6 5.8615639798367525E-5 12-13 0.0010883858571651426 6.927302885261616E-5 0.0 7.993041790686481E-6 6.6608681589054E-5 14-15 0.0011057041143782967 7.326954974795941E-5 0.0 1.065738905424864E-5 8.126259153864589E-5 16-17 0.0011203580243278883 8.525911243398913E-5 0.0 1.1989562686029721E-5 9.458432785645669E-5 18-19 0.001132347587013918 9.591650148823777E-5 0.0 1.3321736317810802E-5 1.1723127959673506E-4 20-21 0.001162987580544883 1.1989562686029722E-4 0.0 1.3321736317810802E-5 1.3188518954632692E-4 22-23 0.0012575719084013397 1.278886686509837E-4 0.0 1.3321736317810802E-5 1.5186779402304315E-4 24-25 0.0017384865894743097 1.3854605770523233E-4 0.0 1.4653909949591883E-5 1.7984344029044582E-4 26-27 0.0035902079376500112 1.5053562039126205E-4 0.0 1.5986083581372962E-5 1.8650430844935123E-4 28-29 0.009281253692618785 1.5186779402304315E-4 0.0 1.9982604476716202E-5 1.9316517660825664E-4 30-31 0.022128736197515524 1.5852866218194855E-4 0.0 2.131477810849728E-5 1.9982604476716202E-4 32-33 0.04819537765057592 1.6518953034085394E-4 0.0 2.2646951740278364E-5 2.0915126018962958E-4 34-35 0.09393822364504287 1.758469193951026E-4 0.0 2.3979125372059443E-5 2.1847647561209715E-4 36-37 0.1644821461423782 1.82507787554008E-4 0.0 2.6643472635621604E-5 2.3979125372059444E-4 38-39 0.2654848865567561 1.9316517660825664E-4 0.0 3.0639993530964845E-5 2.4645212187949985E-4 40-41 0.40059660063925684 1.9982604476716202E-4 0.0 3.596868805808916E-5 2.557773373019674E-4 42-43 0.5683945269511381 2.1048343382141068E-4 0.0 3.730086168987025E-5 2.6510255272443493E-4 44-45 0.7755888240756814 2.1980864924387822E-4 0.0 4.396172984877565E-5 2.690990736197782E-4 46-47 1.0287524367120942 2.4645212187949985E-4 0.0 4.529390348055673E-5 2.7842428904224575E-4 48-49 1.3337762366601127 2.6110603182909176E-4 0.0 4.7958250744118886E-5 3.063999353096484E-4 50-51 1.6827311264633762 2.6510255272443493E-4 0.0 4.7958250744118886E-5 3.183894979956782E-4 52-53 2.0636755016865984 2.690990736197782E-4 0.0 4.7958250744118886E-5 3.3304340794527E-4 54-55 2.484692991927427 2.7575994177868356E-4 0.0 4.9290424375899965E-5 3.3837210247239437E-4 56-57 2.954017762403902 2.82420809937589E-4 0.0 5.595129253480537E-5 3.450329706312997E-4 58-59 3.476189860853132 2.970747198871809E-4 0.0 5.595129253480537E-5 3.5968688058089164E-4 60-61 4.043878335779426 3.063999353096485E-4 2.66434726356216E-6 5.595129253480537E-5 3.6501557510801595E-4 62-63 4.6401192881556845 3.1306080346855383E-4 2.66434726356216E-6 6.794085522083508E-5 3.7034426963514027E-4 64-65 5.260941508385567 3.2371819252280246E-4 2.66434726356216E-6 6.927302885261616E-5 3.8100165868938896E-4 66-67 5.917861637515822 3.2505036615458355E-4 2.66434726356216E-6 6.927302885261616E-5 3.836660059529511E-4 68-69 6.611022218125049 3.2904688704992683E-4 2.66434726356216E-6 7.193737611617834E-5 4.1164165222035376E-4 70-71 7.332105158056407 3.343755815770511E-4 2.66434726356216E-6 7.593389701152157E-5 4.236312149063835E-4 72-73 8.074505540443527 3.423686233677376E-4 2.66434726356216E-6 7.993041790686481E-5 4.5027468754200506E-4 74-75 8.833893800983118 3.5835470694911054E-4 2.66434726356216E-6 8.126259153864589E-5 4.5427120843734834E-4 76-77 9.610660266549292 3.7034426963514027E-4 2.66434726356216E-6 8.259476517042697E-5 4.5959990296447266E-4 78-79 10.40881211542645 3.8633035321651327E-4 2.66434726356216E-6 8.525911243398913E-5 4.6892511838694025E-4 80-81 11.223757345105842 3.98319915902543E-4 2.66434726356216E-6 8.525911243398913E-5 4.7425381291406457E-4 82-83 12.052024311103041 4.1563817311569704E-4 2.66434726356216E-6 8.65912860657702E-5 4.795825074411889E-4 84-85 12.889196857828699 4.342886039606321E-4 2.66434726356216E-6 8.79234596975513E-5 4.915720701272186E-4 86-87 13.736645791949915 4.4761034027844293E-4 2.66434726356216E-6 8.925563332933238E-5 5.022294591814672E-4 88 14.37884007375446 4.5560338206912944E-4 2.66434726356216E-6 9.058780696111346E-5 5.088903273403726E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 250685 0.0 84.56586 8 GTGGTAT 253695 0.0 83.630974 6 AGTGGTA 254310 0.0 83.4672 5 TGGTATC 254310 0.0 83.08112 7 GTATCAA 255105 0.0 82.99098 9 AAGCAGT 259335 0.0 82.312675 1 GCAGTGG 264785 0.0 80.655075 3 CAGTGGT 267985 0.0 79.09637 4 AGCAGTG 272665 0.0 78.37447 2 CAACGCA 247435 0.0 44.489426 12-13 ATCAACG 249960 0.0 44.164444 10-11 ACGCAGA 250885 0.0 43.90833 14-15 AGAGTAC 250435 0.0 43.35424 18-19 TCAACGC 247320 0.0 43.001896 12-13 AACGCAG 249225 0.0 42.763332 14-15 GAGTACT 244480 0.0 42.557037 20-21 CGCAGAG 253020 0.0 42.051064 16-17 AGTACTT 256155 0.0 41.98634 20-21 TATCAAC 255750 0.0 41.375446 10-11 GTACTTT 255960 0.0 40.627747 22-23 >>END_MODULE