FastQCFastQC Report
Thu 26 May 2016
SRR1660723_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660723_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37532645
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT18999045.062004023430803No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT585890.1561014418248434No Hit
TAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT418920.11161483556514602No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA384590.10246813140933712No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA2544200.083.949358
AAGCAGT2466800.083.490421
GTGGTAT2555500.082.881276
TGGTATC2585400.082.572447
AGTGGTA2568850.082.523765
GTATCAA2589550.082.408269
GCAGTGG2587200.081.7940753
AGCAGTG2655050.079.424032
CAGTGGT2692050.078.536834
CAACGCA2507650.044.1997112-13
ATCAACG2525700.043.94153610-11
ACGCAGA2545450.043.61915214-15
AGAGTAC2569200.043.08402318-19
TCAACGC2502450.042.5818312-13
AACGCAG2524150.042.30986414-15
GAGTACT2502800.042.22553620-21
AGTACTT2625300.041.8224620-21
CGCAGAG2561850.041.6718616-17
TATCAAC2595950.041.06539510-11
GTACTTT2616800.040.3745922-23
Produced by FastQC (version 0.11.5)