##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660723_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 37532645 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.183362856521303 34.0 31.0 34.0 27.0 34.0 2 31.846950248243896 34.0 31.0 34.0 28.0 34.0 3 31.630254329264563 34.0 31.0 34.0 30.0 34.0 4 35.558789475135576 37.0 35.0 37.0 33.0 37.0 5 35.893691824810105 37.0 35.0 37.0 33.0 37.0 6 35.34823884647618 37.0 35.0 37.0 33.0 37.0 7 35.563414435619976 37.0 35.0 37.0 33.0 37.0 8 35.93091312909069 37.0 35.0 37.0 33.0 37.0 9 37.73517901549438 39.0 37.0 39.0 35.0 39.0 10-11 37.760220322868264 39.0 37.5 39.0 35.0 39.0 12-13 37.82944133034056 39.0 38.0 39.0 35.0 39.0 14-15 39.27689581962582 41.0 39.0 41.0 36.0 41.0 16-17 39.245045506385175 41.0 39.0 41.0 36.0 41.0 18-19 39.23853216313425 41.0 39.0 41.0 36.0 41.0 20-21 39.176485923121064 40.5 39.0 41.0 36.0 41.0 22-23 39.057383978667104 40.0 38.5 41.0 35.5 41.0 24-25 39.06635428971233 40.0 39.0 41.0 35.5 41.0 26-27 39.01649009815322 40.0 39.0 41.0 35.0 41.0 28-29 38.93531658906533 40.0 38.5 41.0 35.0 41.0 30-31 38.83835214651139 40.0 38.0 41.0 35.0 41.0 32-33 38.715443036322114 40.0 38.0 41.0 35.0 41.0 34-35 38.62755036315719 40.0 38.0 41.0 34.0 41.0 36-37 38.49885161304246 40.0 38.0 41.0 34.0 41.0 38-39 38.11273938993641 40.0 37.0 41.0 34.0 41.0 40-41 37.979813226592476 40.0 37.0 41.0 33.0 41.0 42-43 37.814981757880375 40.0 37.0 41.0 33.0 41.0 44-45 37.68982604343499 40.0 36.0 41.0 33.0 41.0 46-47 37.53966103374809 40.0 36.0 41.0 33.0 41.0 48-49 37.58120081864734 40.0 36.0 41.0 33.0 41.0 50-51 37.57859600622338 40.0 36.0 41.0 33.0 41.0 52-53 37.3315087572432 40.0 36.0 41.0 32.5 41.0 54-55 36.829485518539926 40.0 35.0 41.0 30.5 41.0 56-57 36.36354762633968 39.0 35.0 41.0 29.0 41.0 58-59 36.2298588468785 39.0 35.0 41.0 29.0 41.0 60-61 36.09015120037503 39.0 35.0 41.0 28.5 41.0 62-63 35.860891884917784 39.0 35.0 41.0 28.0 41.0 64-65 35.60481055891478 38.0 35.0 40.5 28.0 41.0 66-67 35.32974683771954 37.5 35.0 40.0 28.0 41.0 68-69 35.06268479080012 37.0 35.0 40.0 28.0 41.0 70-71 34.762961376689546 36.5 34.0 39.0 27.5 41.0 72-73 34.42278395247657 36.0 34.0 39.0 26.5 41.0 74-75 34.04565957182075 35.5 34.0 38.5 26.0 40.5 76-77 32.34110026085292 34.5 32.0 36.5 24.0 39.0 78-79 33.28330162982118 35.0 33.5 37.0 26.0 39.0 80-81 33.18655979348111 35.0 34.0 36.5 26.0 39.0 82-83 32.92845404314031 35.0 34.0 36.0 26.0 38.0 84-85 32.67452428412653 35.0 34.0 36.0 25.0 37.0 86-87 32.456262355077826 35.0 33.5 35.5 25.0 37.0 88-89 32.27906939412344 35.0 33.0 35.0 25.0 36.5 90-91 32.110859613011556 35.0 33.0 35.0 24.5 36.0 92-93 31.960896800638487 35.0 33.0 35.0 24.0 36.0 94-95 31.847473432794303 35.0 33.0 35.0 24.0 36.0 96-97 31.73847252172076 35.0 33.0 35.0 23.0 35.0 98-99 31.638131072297195 35.0 33.0 35.0 23.0 35.0 100 31.57643978994819 35.0 33.0 35.0 22.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.6003523328281695 1101 2 1.2049217584004772 1101 3 1.538306318414758 1101 4 0.9515936946231704 1101 5 0.5647701156214779 1101 6 1.129834606174967 1101 7 0.8134205637819463 1101 8 0.48179929682569167 1101 9 0.5010001948571272 1101 10-11 0.41317700665722157 1101 12-13 0.38278112577100387 1101 14-15 0.5000198922186527 1101 16-17 0.5359397241904915 1101 18-19 0.5096586695838852 1101 20-21 0.5169972240343057 1101 22-23 0.4964573565325523 1101 24-25 0.4716072803985014 1101 26-27 0.47568306673006333 1101 28-29 0.48217590215953976 1101 30-31 0.4984289186838211 1101 32-33 0.5531267681826577 1101 34-35 0.5312100142764038 1101 36-37 0.5188809400165724 1101 38-39 0.5499871461852877 1101 40-41 0.55530745736786 1101 42-43 0.5743498044255944 1101 44-45 0.5112365481434651 1101 46-47 0.5411522978101289 1101 48-49 0.4875136175462771 1101 50-51 0.4904276262221643 1101 52-53 0.53506717380845 1101 54-55 0.5313534188072069 1101 56-57 0.5504537334236801 1101 58-59 0.5155211341686439 1101 60-61 0.5440330010082164 1101 62-63 0.5034440470751065 1101 64-65 0.48251577326163186 1101 66-67 0.47929203234796347 1101 68-69 0.48842352993853666 1101 70-71 0.4928653232604603 1101 72-73 0.44386250928178583 1101 74-75 0.4379930120430586 1101 76-77 0.3810645910005235 1101 78-79 0.48266789301657553 1101 80-81 0.4679243527888417 1101 82-83 0.46657610213434 1101 84-85 0.42052037729298064 1101 86-87 0.4235747144253068 1101 88-89 0.3708538291232699 1101 90-91 0.38958269806080636 1101 92-93 0.34625103497944565 1101 94-95 0.3146145415546435 1101 96-97 0.2811730561366659 1101 98-99 0.3011040254559916 1101 100 0.27659437902784134 1102 1 1.5351668718523293 1102 2 1.14580797135363 1102 3 1.5145410892862792 1102 4 0.9162694837772918 1102 5 0.5119387843084766 1102 6 1.0806448725310815 1102 7 0.799383550797927 1102 8 0.456074929684533 1102 9 0.48007629406347263 1102 10-11 0.3889719639788112 1102 12-13 0.3743321365041936 1102 14-15 0.5043103713720498 1102 16-17 0.5106744443117321 1102 18-19 0.46501029944576544 1102 20-21 0.44879320534649736 1102 22-23 0.4719882615161666 1102 24-25 0.46921589969452526 1102 26-27 0.4807429630208375 1102 28-29 0.4840050180689275 1102 30-31 0.48482118981582545 1102 32-33 0.49330265360221404 1102 34-35 0.4687495325786273 1102 36-37 0.46369456407656173 1102 38-39 0.512019903338647 1102 40-41 0.49171259410611867 1102 42-43 0.4867436947718886 1102 44-45 0.456924765883052 1102 46-47 0.4792692913812786 1102 48-49 0.47306144597482813 1102 50-51 0.4448303870990955 1102 52-53 0.44640391697698334 1102 54-55 0.519026655965682 1102 56-57 0.5263977201274699 1102 58-59 0.5314231476681002 1102 60-61 0.5545882623010243 1102 62-63 0.5294781419615191 1102 64-65 0.5038927632764896 1102 66-67 0.48783423645311785 1102 68-69 0.44055379964000707 1102 70-71 0.4041611994648733 1102 72-73 0.3972072680679517 1102 74-75 0.4579533424829165 1102 76-77 0.31298473945014393 1102 78-79 0.47794217012902607 1102 80-81 0.48768988339269725 1102 82-83 0.4717245382898412 1102 84-85 0.44705365036130473 1102 86-87 0.42457668002850113 1102 88-89 0.4038790682304736 1102 90-91 0.40871596767974694 1102 92-93 0.39104054513421005 1102 94-95 0.3273099897923366 1102 96-97 0.32410414724095915 1102 98-99 0.33557006342285334 1102 100 0.33739229308949703 1103 1 1.524443228901763 1103 2 1.1207933984866507 1103 3 1.453315154130241 1103 4 0.906233188820103 1103 5 0.5164084656906525 1103 6 1.05372964762757 1103 7 0.7813316242726316 1103 8 0.44556874769232735 1103 9 0.43661850020483683 1103 10-11 0.38107651143933907 1103 12-13 0.33211438077502464 1103 14-15 0.40827798747036326 1103 16-17 0.42169315681024244 1103 18-19 0.39926688917260833 1103 20-21 0.4122994169372589 1103 22-23 0.408134440385048 1103 24-25 0.4130334921191263 1103 26-27 0.42108235989906717 1103 28-29 0.3893262327782452 1103 30-31 0.39301353351742563 1103 32-33 0.4146486353973131 1103 34-35 0.4340546233521039 1103 36-37 0.3932773980682214 1103 38-39 0.37061074235544567 1103 40-41 0.4186523068886743 1103 42-43 0.44466730347703987 1103 44-45 0.42365343627268004 1103 46-47 0.46049225295125495 1103 48-49 0.415616007688854 1103 50-51 0.4006786620982652 1103 52-53 0.40348132790068547 1103 54-55 0.4282527529142257 1103 56-57 0.45671725570067423 1103 58-59 0.4604651499479715 1103 60-61 0.4386128578392885 1103 62-63 0.4048561585790793 1103 64-65 0.3915750455391773 1103 66-67 0.4208093692665855 1103 68-69 0.39401226679257917 1103 70-71 0.3859966444575278 1103 72-73 0.3685912097691002 1103 74-75 0.3988289888585612 1103 76-77 0.279385885843233 1103 78-79 0.41473992609633825 1103 80-81 0.4416940821244566 1103 82-83 0.437341137200562 1103 84-85 0.4137868195930494 1103 86-87 0.3472968501244793 1103 88-89 0.35477854936824116 1103 90-91 0.34128287866180784 1103 92-93 0.3381203905467842 1103 94-95 0.3108577181270924 1103 96-97 0.3126861539580261 1103 98-99 0.31682231939957717 1103 100 0.26831680299204663 1104 1 1.421500657275761 1104 2 1.056680938481616 1104 3 1.3981624779131927 1104 4 0.867613626429609 1104 5 0.4788744804272227 1104 6 0.9998204439239657 1104 7 0.751286121318067 1104 8 0.3964473935933057 1104 9 0.4022864740703298 1104 10-11 0.31333998860144874 1104 12-13 0.272099546173699 1104 14-15 0.3363076588314513 1104 16-17 0.35249964784956234 1104 18-19 0.3341824166465699 1104 20-21 0.32570427204675667 1104 22-23 0.33367693607908677 1104 24-25 0.3260655378554347 1104 26-27 0.325945182352946 1104 28-29 0.29918755241563133 1104 30-31 0.3287261351233397 1104 32-33 0.34618874319104265 1104 34-35 0.36432201955188503 1104 36-37 0.3408250499363774 1104 38-39 0.37428599686077035 1104 40-41 0.3424971491052986 1104 42-43 0.3478419756986355 1104 44-45 0.3262847629129979 1104 46-47 0.35774283245424954 1104 48-49 0.30659712866863487 1104 50-51 0.29500280956474256 1104 52-53 0.3149309140425913 1104 54-55 0.33155409706595407 1104 56-57 0.40255662897772027 1104 58-59 0.40931052263697865 1104 60-61 0.4250965869937886 1104 62-63 0.3649560202002107 1104 64-65 0.32354594738416864 1104 66-67 0.34567150258505563 1104 68-69 0.2966093272671557 1104 70-71 0.2939781814773639 1104 72-73 0.2897722416364914 1104 74-75 0.3267414602023919 1104 76-77 0.20474258826469338 1104 78-79 0.3284917771577298 1104 80-81 0.3136129118607869 1104 82-83 0.3321465071023866 1104 84-85 0.30668427486961747 1104 86-87 0.31838814556100203 1104 88-89 0.2808108690702582 1104 90-91 0.30425734355791434 1104 92-93 0.30680707340282254 1104 94-95 0.29072541753253134 1104 96-97 0.26728590911042716 1104 98-99 0.28315611834753085 1104 100 0.31672397329362667 1105 1 1.39536531048903 1105 2 1.0334621080086315 1105 3 1.3498645413019865 1105 4 0.8480547960659734 1105 5 0.4247646403298262 1105 6 0.9865381075671706 1105 7 0.7144555221297892 1105 8 0.3749337019097325 1105 9 0.3583801412053731 1105 10-11 0.2529833719795249 1105 12-13 0.2412245001565907 1105 14-15 0.33761659489774587 1105 16-17 0.3179287505251125 1105 18-19 0.30440044204802064 1105 20-21 0.31774516940765096 1105 22-23 0.30112924231062266 1105 24-25 0.2957403734695774 1105 26-27 0.31729932566935304 1105 28-29 0.32353464486354255 1105 30-31 0.3041251968300074 1105 32-33 0.3514019060423905 1105 34-35 0.354401990219408 1105 36-37 0.3486912242200191 1105 38-39 0.36352985152689854 1105 40-41 0.36359911298778513 1105 42-43 0.34939077592790113 1105 44-45 0.33219822296194224 1105 46-47 0.33761783222644226 1105 48-49 0.3338185702706511 1105 50-51 0.36049388725802345 1105 52-53 0.3415671581628814 1105 54-55 0.36387224634909643 1105 56-57 0.3690015535643383 1105 58-59 0.3388186783716165 1105 60-61 0.30879689535395727 1105 62-63 0.3162893316640236 1105 64-65 0.26901629801628957 1105 66-67 0.2715394091165706 1105 68-69 0.26807842564169704 1105 70-71 0.2723671134977934 1105 72-73 0.24834557581726102 1105 74-75 0.2841552886159988 1105 76-77 0.19567429113452306 1105 78-79 0.2744485365693592 1105 80-81 0.29483677095549155 1105 82-83 0.27426073568256726 1105 84-85 0.28287018921583496 1105 86-87 0.2938047427463957 1105 88-89 0.2830383703505319 1105 90-91 0.2903835165384976 1105 92-93 0.2742062378506489 1105 94-95 0.24249421141963623 1105 96-97 0.2263639905707251 1105 98-99 0.24110027112250165 1105 100 0.22325020122136507 1106 1 1.3781536255780686 1106 2 1.0108734726167903 1106 3 1.3312875562588538 1106 4 0.7913628139488367 1106 5 0.4031158865653168 1106 6 0.9543959010507876 1106 7 0.6787629776767972 1106 8 0.32749238780964873 1106 9 0.30486398548114835 1106 10-11 0.2158914880765863 1106 12-13 0.1908728684705281 1106 14-15 0.25229131084760326 1106 16-17 0.2443322491199993 1106 18-19 0.22011549756895477 1106 20-21 0.24363519906162878 1106 22-23 0.27099055185267673 1106 24-25 0.2574091084595622 1106 26-27 0.2179798703203275 1106 28-29 0.22557861833548998 1106 30-31 0.24847801137932635 1106 32-33 0.30102315478552555 1106 34-35 0.2875406843865349 1106 36-37 0.2905827294583503 1106 38-39 0.29953705596857816 1106 40-41 0.31769205820236834 1106 42-43 0.35640972369098733 1106 44-45 0.30771470789100164 1106 46-47 0.3270711557967374 1106 48-49 0.30772056816821447 1106 50-51 0.3015694780146987 1106 52-53 0.29455913241169185 1106 54-55 0.3513002077089382 1106 56-57 0.3810261356310747 1106 58-59 0.35866296977493306 1106 60-61 0.3576381539258122 1106 62-63 0.3152716424071187 1106 64-65 0.2710963462939944 1106 66-67 0.25311991368409537 1106 68-69 0.24653972808158642 1106 70-71 0.24519271374023788 1106 72-73 0.1823574593577959 1106 74-75 0.19945780286776227 1106 76-77 0.11763699345503653 1106 78-79 0.2521843479261108 1106 80-81 0.3137725369751365 1106 82-83 0.314709724499572 1106 84-85 0.2929803697689337 1106 86-87 0.29903608515055424 1106 88-89 0.31021837036026056 1106 90-91 0.3342093540467843 1106 92-93 0.3393462870234174 1106 94-95 0.3234746681205074 1106 96-97 0.3321192742657644 1106 98-99 0.3283000602100721 1106 100 0.3137775175610642 1107 1 1.2945628377872431 1107 2 0.9197936327725209 1107 3 1.2702679064176685 1107 4 0.7688416999002925 1107 5 0.3820033946278656 1107 6 0.9280364750053565 1107 7 0.6328877631825165 1107 8 0.26772876949244306 1107 9 0.2819995673758626 1107 10-11 0.18950438968643368 1107 12-13 0.12963031863554164 1107 14-15 0.1727124116224914 1107 16-17 0.18272382433089973 1107 18-19 0.14511750701561255 1107 20-21 0.14059816099733524 1107 22-23 0.13581052524307324 1107 24-25 0.17027440675908423 1107 26-27 0.12441525769494177 1107 28-29 0.15508684022708508 1107 30-31 0.15255684140588954 1107 32-33 0.20149128510295355 1107 34-35 0.2193223050816826 1107 36-37 0.18673270748179505 1107 38-39 0.1894645559820205 1107 40-41 0.19767936331079738 1107 42-43 0.1986422429908714 1107 44-45 0.20230790248950115 1107 46-47 0.22171256296412167 1107 48-49 0.21384173893410008 1107 50-51 0.17684410710905496 1107 52-53 0.16432921492070562 1107 54-55 0.20714432955385575 1107 56-57 0.18448677778796707 1107 58-59 0.15694574901514358 1107 60-61 0.1317880306278596 1107 62-63 0.0998015493414357 1107 64-65 0.10705400046030178 1107 66-67 0.10838520875717705 1107 68-69 0.12838158264802502 1107 70-71 0.1340673061551101 1107 72-73 0.10523587991007588 1107 74-75 0.14455757613990983 1107 76-77 0.014346602374260442 1107 78-79 0.13167530555764273 1107 80-81 0.2043514161057871 1107 82-83 0.20652185199131878 1107 84-85 0.19814433637136375 1107 86-87 0.13841030100645213 1107 88-89 0.18868168706236332 1107 90-91 0.18782378255307464 1107 92-93 0.19422965279562732 1107 94-95 0.1895092954148474 1107 96-97 0.22302234648329033 1107 98-99 0.24412997866333086 1107 100 0.23781964122354182 1108 1 1.2766888651865393 1108 2 0.8983017210463906 1108 3 1.2415889083499145 1108 4 0.7503390386157776 1108 5 0.34480751456084846 1108 6 0.8813987489619777 1108 7 0.626557765603053 1108 8 0.2601273981291712 1108 9 0.20617658255269333 1108 10-11 0.1340263358797742 1108 12-13 0.12039269490630033 1108 14-15 0.1434338797000052 1108 16-17 0.1447532975072292 1108 18-19 0.1219902831448394 1108 20-21 0.13784937581175427 1108 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98-99 -1.146413264191839 2316 100 -1.076326148222222 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 87.0 7 1070.0 8 1370.0 9 521.0 10 508.0 11 905.0 12 1291.0 13 2147.0 14 5054.0 15 12442.0 16 24883.0 17 36195.0 18 44571.0 19 57807.0 20 78496.0 21 112424.0 22 169928.0 23 282283.0 24 472315.0 25 627020.0 26 633503.0 27 527699.0 28 470029.0 29 492118.0 30 568273.0 31 691038.0 32 852460.0 33 1088588.0 34 1486714.0 35 2288400.0 36 4097957.0 37 8176397.0 38 1.0911675E7 39 3309393.0 40 7084.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 15.11422773499063 32.20371623207476 28.509139705095333 24.17291632783928 2 20.664476397461012 33.50518496523417 28.01279032519961 17.81754831210521 3 24.68664930156158 26.060613636492036 27.347357606688693 21.90537945525769 4 18.624602639339905 28.80388829483822 25.384530391244738 27.186978674577144 5 21.587824290166296 32.634900155808424 26.652819870411953 19.124455683613316 6 28.293282884786585 27.758252523704112 25.780347646364955 18.168116945144348 7 20.619515557274017 26.446232060764498 32.5404675057306 20.39378487623089 8 26.304635784880446 26.985141215047932 26.37858227782268 20.331640722248938 9 25.523464320818967 25.260109572882744 28.50507147857742 20.711354627720873 10-11 20.31831300650196 29.91215653723475 29.501320538097843 20.268209918165443 12-13 20.23510333471036 27.044968453462314 29.268915100441227 23.4510131113861 14-15 20.187458144769707 33.04415929119837 26.368100356369766 20.40028220766216 16-17 23.387350993831834 26.752318798847906 26.419912059192757 23.4404181481275 18-19 20.24598053241385 29.86618182651396 26.48176940367512 23.406068237397072 20-21 23.308875034741536 29.982252592208464 26.421995046509462 20.28687732654054 22-23 23.253173212384702 26.949854137578644 29.468015136057087 20.32895751397957 24-25 20.312870783221655 29.792341479779655 26.30160168427012 23.59318605272857 26-27 20.3729086194752 26.777277693486003 32.507789923138766 20.342023763900034 28-29 20.360219847087258 26.887568899721543 32.45481787763898 20.297393375552222 30-31 20.30174925311477 26.7749828326441 32.48234930429443 20.440918609946696 32-33 20.478107936619942 26.919477241063248 32.23677311951221 20.3656417028046 34-35 20.463979672404605 27.057139759719217 32.133137819933395 20.34574274794278 36-37 20.40691772739586 26.986111665842056 32.21114113304607 20.395829473716017 38-39 20.433996380954348 26.993448348331643 32.2374075296533 20.335147741060712 40-41 20.4666893928012 27.01964997942484 32.21157117300281 20.30208945477115 42-43 20.447418439412214 27.073943173711406 32.14142387676475 20.33721451011163 44-45 20.509125435372123 27.13455224534083 32.089918244837925 20.26640407444912 46-47 20.518327800582373 27.25657428286523 31.989884172835474 20.23521374371692 48-49 20.57005305309086 27.27168037295249 31.87456768739641 20.283698886560238 50-51 20.61662280140496 27.35041869350759 31.74531117299565 20.287647332091797 52-53 20.721868978192827 27.483606074763784 31.47960231510395 20.314922631939442 54-55 21.02577173878453 27.715253517762545 30.777833664987437 20.48114107846548 56-57 21.462046128630075 28.271755615989303 29.654137608153952 20.61206064722667 58-59 21.52976457550342 28.795937789067615 28.966816328121332 20.707481307307628 60-61 21.550791323705674 29.074190164105502 28.62163002049395 20.753388491694874 62-63 21.513856187563754 29.231676727808235 28.421502022215435 20.832965062412576 64-65 21.503475137198915 29.311968282151103 28.325298271114413 20.859258309535573 66-67 21.442287223365867 29.215650900067715 28.435957584751886 20.906104291814536 68-69 21.393292238210254 29.099965996346498 28.558985089573873 20.94775667586937 70-71 21.336537778767564 29.03290666978692 28.59759870805914 21.032956843386373 72-73 21.256270760365567 28.991572189252345 28.547443723777942 21.20471332660414 74-75 21.31182807939339 29.08250583845283 28.353199266603866 21.25246681554992 76-77 21.340727956671657 29.193537005254594 28.13096279049444 21.334772247579306 78-79 21.377160933888785 29.377351271546857 27.76860618918617 21.476881605378185 80-81 21.38989302480392 29.55546799522365 27.436331580722744 21.61830739924968 82-83 21.340699337988287 29.696150508683385 27.106430289674204 21.856719863654124 84-85 21.288978187137737 29.743747477257532 26.900591724557017 22.06668261104772 86-87 21.27597935512924 29.781135007757833 26.734867169054176 22.208018468058754 88-89 21.228942184022184 29.83660248688543 26.585537828685407 22.348917500406976 90-91 21.220812902991373 29.805457498785888 26.43778836322717 22.53594123499557 92-93 21.177645847524985 29.830877813825822 26.34411800741218 22.647358331237008 94-95 21.137723578575425 29.804140252594426 26.248821393718075 22.809314775112068 96-97 21.108949568071708 29.761260107972575 26.190747548533565 22.939042775422152 98-99 21.086249342575858 29.736561594802712 26.168638926874284 23.008550135747146 100 21.023630791950634 29.69005662027962 26.126876684518336 23.159435903251413 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 38506.0 1 29314.0 2 15997.5 3 10984.5 4 9894.5 5 9608.0 6 9881.0 7 10121.5 8 10177.0 9 10566.0 10 11737.5 11 22618.0 12 163272.0 13 190941.0 14 85998.0 15 90361.5 16 103291.5 17 116004.0 18 127464.0 19 139361.0 20 155646.0 21 181197.0 22 217268.0 23 255632.5 24 292787.5 25 336314.0 26 386522.0 27 445503.5 28 522016.5 29 595937.0 30 654197.5 31 717733.0 32 788526.5 33 848455.0 34 897447.0 35 958504.0 36 1030933.0 37 1082792.0 38 1121533.5 39 1152567.5 40 1165541.5 41 1182841.0 42 1216683.5 43 1230905.0 44 1225817.0 45 1227874.0 46 1226222.5 47 1224810.5 48 1216106.0 49 1199033.5 50 1184951.0 51 1167315.5 52 1137566.0 53 1089084.0 54 1024710.5 55 951627.5 56 868407.5 57 778753.5 58 687047.5 59 587922.0 60 486131.5 61 395370.5 62 313017.5 63 235675.5 64 174232.5 65 127578.0 66 91886.5 67 69083.5 68 52134.0 69 38586.0 70 29720.5 71 24016.5 72 20001.0 73 15378.0 74 11386.5 75 9119.0 76 6796.0 77 4075.5 78 2328.0 79 1548.0 80 959.0 81 645.5 82 445.5 83 304.0 84 210.5 85 135.5 86 91.0 87 67.0 88 50.0 89 30.5 90 17.0 91 14.5 92 11.0 93 8.0 94 4.5 95 4.0 96 3.0 97 1.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.33095722403790084 2 0.0064024264743398705 3 2.379970822733117 4 8.152902626500212E-4 5 4.262955621699457E-5 6 0.008488610381709042 7 0.007902453983725367 8 2.6909907361977815E-4 9 2.6643472635621606E-6 10-11 7.593389701152157E-5 12-13 0.0 14-15 0.0 16-17 3.3304340794527E-5 18-19 0.0 20-21 3.2371819252280246E-4 22-23 4.529390348055673E-5 24-25 4.942364173907807E-4 26-27 0.0034783053525804003 28-29 0.00609203001813488 30-31 0.005629765767906844 32-33 0.015522487157513146 34-35 0.010016613537361942 36-37 0.005163504996783467 38-39 0.005415285813190091 40-41 0.008433991262806018 42-43 0.019219268985705643 44-45 0.03429947449746747 46-47 0.03312050083334121 48-49 0.03981600550667292 50-51 0.04024762976336999 52-53 0.06234705814098633 54-55 0.03365337028605365 56-57 0.022265950081588975 58-59 0.01673876168332927 60-61 0.01783380840865332 62-63 0.02482771997550399 64-65 0.04492089486365802 66-67 0.048019530731180815 68-69 0.050699864078324344 70-71 0.06095626886940688 72-73 0.06908519237053504 74-75 0.05993049517293546 76-77 0.059729336954536506 78-79 0.05425277115428449 80-81 0.05185619079071033 82-83 0.04809679680182412 84-85 0.05037481371216976 86-87 0.0581533755481395 88-89 0.06491149238216491 90-91 0.06066585501767861 92-93 0.06534711315975733 94-95 0.07338145233302902 96-97 0.07270337595445245 98-99 0.04758657430085196 100 0.0070045689559049195 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3.7532645E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 38.357592062312236 #Duplication Level Percentage of deduplicated Percentage of total 1 68.74856934263576 26.37029577712408 2 16.010955263353704 12.282833810393047 3 5.5969129231396035 6.440523081422173 4 2.608303977614237 4.001930397913331 5 1.5326279208500786 2.939395828563854 6 1.0507432010553992 2.4182387440998676 7 0.7218457340222711 1.9381784938282631 8 0.5200167262843569 1.5957271561915554 9 0.4017598470534598 1.386948628826421 >10 2.411741904289708 17.009134583323203 >50 0.2233401685972871 6.027085263021403 >100 0.17158868656749598 11.32774190027097 >500 0.0010421358266282021 0.2415392294903656 >1k 4.688484125016788E-4 0.3993356590638841 >5k 4.166014986143054E-5 0.11547557872063641 >10k+ 4.166014756252347E-5 5.505615867747006 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 1899904 5.062004023430803 No Hit AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 58589 0.1561014418248434 No Hit TAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 41892 0.11161483556514602 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 38459 0.10246813140933712 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.001012451960153621 0.0 0.0 2.66434726356216E-6 2.131477810849728E-5 2 0.0010204450019443074 1.065738905424864E-5 0.0 2.66434726356216E-6 2.3979125372059443E-5 3 0.0010231093492078696 1.3321736317810802E-5 0.0 2.66434726356216E-6 3.1972167162745924E-5 4 0.0010257736964714317 2.3979125372059443E-5 0.0 2.66434726356216E-6 3.9965208953432405E-5 5 0.0010257736964714317 2.9307819899183762E-5 0.0 2.66434726356216E-6 4.262955621699456E-5 6 0.001028438043734994 3.463651442630808E-5 0.0 2.66434726356216E-6 5.0622598007681044E-5 7 0.001028438043734994 3.463651442630808E-5 0.0 2.66434726356216E-6 5.328694527124321E-5 8 0.0010337667382621183 3.463651442630808E-5 0.0 2.66434726356216E-6 5.8615639798367525E-5 9 0.0010337667382621183 3.9965208953432405E-5 0.0 2.66434726356216E-6 5.8615639798367525E-5 10-11 0.001045756300948148 4.396172984877565E-5 0.0 3.9965208953432405E-6 6.794085522083508E-5 12-13 0.0010684032526884263 4.7958250744118886E-5 0.0 5.32869452712432E-6 7.326954974795941E-5 14-15 0.0010870536835333614 5.328694527124321E-5 0.0 1.1989562686029721E-5 8.126259153864589E-5 16-17 0.0011003754198511723 5.861563979836753E-5 2.66434726356216E-6 2.3979125372059443E-5 9.991302238358102E-5 18-19 0.001113697156168983 6.794085522083508E-5 5.32869452712432E-6 2.6643472635621604E-5 1.1323475870139181E-4 20-21 0.0011456693233317288 7.46017233797405E-5 5.32869452712432E-6 3.596868805808916E-5 1.33217363178108E-4 22-23 0.0012402536511881856 8.525911243398913E-5 5.32869452712432E-6 4.1297382585213484E-5 1.545321412866053E-4 24-25 0.0017238326795247178 9.724867512001885E-5 5.32869452712432E-6 4.662607711233781E-5 1.66521703972635E-4 26-27 0.003575554027700419 1.0923823780604858E-4 5.32869452712432E-6 4.7958250744118886E-5 1.758469193951026E-4 28-29 0.009307897165254406 1.1589910596495397E-4 5.32869452712432E-6 5.0622598007681044E-5 1.8517213481757013E-4 30-31 0.022353873541286526 1.2255997412385938E-4 5.32869452712432E-6 5.461911890302428E-5 1.944973502400377E-4 32-33 0.048742901013237944 1.278886686509837E-4 5.32869452712432E-6 6.394433432549185E-5 2.1048343382141068E-4 34-35 0.09531435900667272 1.3055301591454585E-4 5.32869452712432E-6 6.6608681589054E-5 2.2513734377100253E-4 36-37 0.1669306812775918 1.3854605770523233E-4 5.32869452712432E-6 6.927302885261616E-5 2.3179821192990794E-4 38-39 0.2694014770341925 1.545321412866053E-4 5.32869452712432E-6 7.060520248439726E-5 2.4511994824771876E-4 40-41 0.4061277855584119 1.6252518307729178E-4 5.32869452712432E-6 7.726607064330265E-5 2.584416845655296E-4 42-43 0.5756109115144963 1.731825721315404E-4 5.32869452712432E-6 8.126259153864589E-5 2.7709211541046466E-4 44-45 0.7845130552349828 1.82507787554008E-4 5.32869452712432E-6 8.259476517042697E-5 2.9041385172827547E-4 46-47 1.0398227995921951 1.8916865571291339E-4 5.32869452712432E-6 8.259476517042697E-5 3.037355880460863E-4 48-49 1.3469540982256913 1.944973502400377E-4 5.32869452712432E-6 8.925563332933238E-5 3.2371819252280246E-4 50-51 1.6980604484442812 1.9849387113538095E-4 5.32869452712432E-6 9.058780696111346E-5 3.3837210247239437E-4 52-53 2.0821420925703475 2.0648691292606743E-4 5.32869452712432E-6 9.858084875179993E-5 3.437007969995187E-4 54-55 2.5070615193786634 2.1048343382141068E-4 5.32869452712432E-6 9.858084875179993E-5 3.530260124219863E-4 56-57 2.981163464498705 2.1714430198031609E-4 5.32869452712432E-6 9.858084875179993E-5 3.7034426963514027E-4 58-59 3.5089866434939503 2.3712690645703226E-4 5.32869452712432E-6 9.858084875179993E-5 3.7833731142582677E-4 60-61 4.082897701454294 2.49116469143062E-4 5.32869452712432E-6 1.0124519601536209E-4 3.8233383232117E-4 62-63 4.684414061412405 2.557773373019674E-4 5.32869452712432E-6 1.0124519601536209E-4 3.8766252684829436E-4 64-65 5.311023243898744 2.5977385819731067E-4 5.32869452712432E-6 1.0923823780604858E-4 4.0231643679788617E-4 66-67 5.974790745496353 2.624382054608728E-4 5.32869452712432E-6 1.1190258506961073E-4 4.129738258521349E-4 68-69 6.675845787047516 2.6510255272443493E-4 5.32869452712432E-6 1.1589910596495397E-4 4.302920830652889E-4 70-71 7.405193532190443 2.7442776814690247E-4 5.32869452712432E-6 1.1723127959673505E-4 4.449459930148808E-4 72-73 8.156045224097582 2.9174602536005657E-4 5.32869452712432E-6 1.2122780049207829E-4 4.5560338206912944E-4 74-75 8.924414999262641 3.050677616778674E-4 5.32869452712432E-6 1.278886686509837E-4 4.7425381291406457E-4 76-77 9.71116877054628 3.1039645620499164E-4 5.32869452712432E-6 1.3055301591454585E-4 4.8091468107297E-4 78-79 10.51866315310312 3.143929771003349E-4 5.32869452712432E-6 1.345495368098891E-4 4.8757554923187533E-4 80-81 11.342215556617447 3.2505036615458355E-4 5.32869452712432E-6 1.3721388407345124E-4 4.98232938286124E-4 82-83 12.179994242345563 3.423686233677376E-4 5.32869452712432E-6 1.3987823133701342E-4 5.102225009721538E-4 84-85 13.027808192041888 3.516938387902052E-4 5.32869452712432E-6 1.4387475223235664E-4 5.235442372899645E-4 86-87 13.8863314855641 3.7833731142582677E-4 5.32869452712432E-6 1.4787127312769992E-4 5.48855536293805E-4 88 14.537230189878704 3.889947004800754E-4 5.32869452712432E-6 1.49203446759481E-4 5.568485780844915E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 254420 0.0 83.94935 8 AAGCAGT 246680 0.0 83.49042 1 GTGGTAT 255550 0.0 82.88127 6 TGGTATC 258540 0.0 82.57244 7 AGTGGTA 256885 0.0 82.52376 5 GTATCAA 258955 0.0 82.40826 9 GCAGTGG 258720 0.0 81.794075 3 AGCAGTG 265505 0.0 79.42403 2 CAGTGGT 269205 0.0 78.53683 4 CAACGCA 250765 0.0 44.19971 12-13 ATCAACG 252570 0.0 43.941536 10-11 ACGCAGA 254545 0.0 43.619152 14-15 AGAGTAC 256920 0.0 43.084023 18-19 TCAACGC 250245 0.0 42.58183 12-13 AACGCAG 252415 0.0 42.309864 14-15 GAGTACT 250280 0.0 42.225536 20-21 AGTACTT 262530 0.0 41.82246 20-21 CGCAGAG 256185 0.0 41.67186 16-17 TATCAAC 259595 0.0 41.065395 10-11 GTACTTT 261680 0.0 40.37459 22-23 >>END_MODULE