FastQCFastQC Report
Thu 26 May 2016
SRR1660722_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660722_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25175132
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT14230715.652685356327029No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT359060.14262487283085548No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1847750.082.676568
AGTGGTA1850100.082.6457445
AAGCAGT1869250.082.366631
TGGTATC1862000.081.7826847
GTGGTAT1881700.081.2527546
GTATCAA1880200.081.1407859
GCAGTGG1901600.081.0053253
AGCAGTG1957000.078.7756962
CAGTGGT1948900.078.421934
CAACGCA1829600.043.13005412-13
GAGTACT1745350.042.8969920-21
ACGCAGA1842650.042.85794414-15
ATCAACG1846100.042.85632310-11
AGAGTAC1833000.042.4409318-19
AGTACTT1820000.042.39016320-21
TCAACGC1829300.041.7635912-13
AACGCAG1834750.041.76126514-15
CGCAGAG1850250.041.32921616-17
GTACTTT1831100.040.9064322-23
TATCAAC1882800.040.42953510-11
Produced by FastQC (version 0.11.5)