##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660722_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 25175132 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.836308186983885 34.0 31.0 34.0 30.0 34.0 2 31.962257953602787 34.0 31.0 34.0 30.0 34.0 3 32.02416984347887 34.0 31.0 34.0 30.0 34.0 4 35.42942726179152 37.0 35.0 37.0 33.0 37.0 5 35.39787755631232 37.0 35.0 37.0 33.0 37.0 6 35.47213027522557 37.0 35.0 37.0 33.0 37.0 7 35.41930338240133 37.0 35.0 37.0 33.0 37.0 8 35.43138840344511 37.0 35.0 37.0 33.0 37.0 9 37.104810731478985 39.0 37.0 39.0 33.0 39.0 10-11 37.04245602366653 39.0 37.0 39.0 33.0 39.0 12-13 37.071061295726274 39.0 37.0 39.0 33.0 39.0 14-15 38.44035052130015 40.0 38.0 41.0 33.0 41.0 16-17 38.41722919268109 40.0 38.0 41.0 33.0 41.0 18-19 38.379332867053094 40.0 38.0 41.0 33.5 41.0 20-21 38.25442557758982 40.0 38.0 41.0 33.5 41.0 22-23 38.078467552821564 40.0 38.0 41.0 32.5 41.0 24-25 38.069007244927256 40.0 38.0 41.0 33.0 41.0 26-27 38.05573323309685 40.0 38.0 41.0 33.0 41.0 28-29 37.97106174060974 40.0 38.0 41.0 33.0 41.0 30-31 37.87772934020764 40.0 38.0 41.0 32.0 41.0 32-33 37.75098019347028 40.0 38.0 41.0 32.0 41.0 34-35 37.59404349895762 40.0 37.5 41.0 31.5 41.0 36-37 37.4012622058943 40.0 37.0 41.0 31.0 41.0 38-39 36.968929815343174 39.5 35.0 41.0 30.5 41.0 40-41 36.905374597440044 39.0 35.0 41.0 30.0 41.0 42-43 36.91007075950982 40.0 35.0 41.0 30.0 41.0 44-45 36.90872157095343 40.0 35.0 41.0 30.0 41.0 46-47 36.99772382524151 40.0 35.0 41.0 31.0 41.0 48-49 36.88739733320961 40.0 35.0 41.0 30.0 41.0 50-51 35.51860907819669 38.5 34.0 40.0 28.0 40.5 52-53 35.671108298458975 38.5 34.5 39.5 28.0 40.5 54-55 36.03963154989614 39.0 35.0 41.0 27.0 41.0 56-57 35.64976082349836 39.0 35.0 41.0 24.5 41.0 58-59 35.453138478082266 39.0 35.0 41.0 24.0 41.0 60-61 35.233606203137285 39.0 35.0 41.0 23.0 41.0 62-63 35.01064397596803 38.5 35.0 41.0 23.0 41.0 64-65 34.76886905697257 38.0 34.0 40.5 23.0 41.0 66-67 34.51264692475098 37.0 34.0 40.0 23.0 41.0 68-69 34.24657090179309 37.0 34.0 40.0 23.0 41.0 70-71 33.95396341913917 36.0 34.0 39.0 23.0 41.0 72-73 33.619264677539725 36.0 33.5 39.0 23.0 41.0 74-75 33.24766863188642 35.0 33.0 38.5 21.5 40.5 76-77 32.856937631945684 35.0 33.0 37.0 20.0 39.5 78-79 32.49016773775009 35.0 33.0 37.0 20.0 39.0 80-81 32.17024486703784 35.0 32.5 36.5 20.0 39.0 82-83 31.858972517006066 35.0 32.0 36.0 19.0 37.5 84-85 31.574559291287926 35.0 32.0 36.0 18.5 37.0 86-87 31.357918739015947 35.0 32.0 35.0 18.0 37.0 88-89 31.15253840575692 35.0 32.0 35.0 18.0 36.0 90-91 30.981191538538905 35.0 31.0 35.0 17.5 36.0 92-93 30.82879891156082 35.0 31.0 35.0 17.0 36.0 94-95 30.64963798402328 35.0 31.0 35.0 15.0 35.5 96-97 30.513638836134007 35.0 31.0 35.0 15.0 35.0 98-99 30.407920343774165 35.0 31.0 35.0 15.0 35.0 100 30.32687010340204 34.0 31.0 35.0 15.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5632177376446137 1101 2 0.5854523439754473 1101 3 0.5572410731784814 1101 4 0.49068391598234484 1101 5 0.490863565903112 1101 6 0.46642853074742163 1101 7 0.47568781079934297 1101 8 0.4907184515968481 1101 9 0.5787916201980252 1101 10-11 0.6014642486516166 1101 12-13 0.6073328700572134 1101 14-15 0.724453475830316 1101 16-17 0.6780553789117292 1101 18-19 0.6888816330880587 1101 20-21 0.710604642157584 1101 22-23 0.7500886101104669 1101 24-25 0.7456997942811654 1101 26-27 0.6987794137893175 1101 28-29 0.6831015870951731 1101 30-31 0.6606902201955265 1101 32-33 0.6742997468498686 1101 34-35 0.6834272811602773 1101 36-37 0.6426300299009782 1101 38-39 0.653456031636793 1101 40-41 0.6698949253399817 1101 42-43 0.6750936096075932 1101 44-45 0.7078009877677687 1101 46-47 0.7371001473344378 1101 48-49 0.739153525535734 1101 50-51 0.7200015238883637 1101 52-53 0.7490075238832503 1101 54-55 0.75049970029891 1101 56-57 0.7563780731380163 1101 58-59 0.6649264802172326 1101 60-61 0.6705016586786172 1101 62-63 0.6265435631274201 1101 64-65 0.6111143276340485 1101 66-67 0.6139637448746598 1101 68-69 0.5806813239929198 1101 70-71 0.5810644416578086 1101 72-73 0.5622192303422437 1101 74-75 0.48777159307189066 1101 76-77 0.49447853441011347 1101 78-79 0.5306201227091094 1101 80-81 0.4245554237311566 1101 82-83 0.4481994198276489 1101 84-85 0.4069548433710217 1101 86-87 0.3950034955044792 1101 88-89 0.3549062584863165 1101 90-91 0.3286935288692767 1101 92-93 0.36464262620874166 1101 94-95 0.3809059161849788 1101 96-97 0.3540567545551916 1101 98-99 0.3349951011288752 1101 100 0.28263074425009194 1102 1 0.46095424943717234 1102 2 0.5034355887185242 1102 3 0.5162382735784803 1102 4 0.4482647872368801 1102 5 0.45057814442442634 1102 6 0.41296912482278003 1102 7 0.4335826555533657 1102 8 0.43399920039851736 1102 9 0.4748256704796887 1102 10-11 0.4921906667432765 1102 12-13 0.49650754256100527 1102 14-15 0.5911552629878614 1102 16-17 0.5700206027200494 1102 18-19 0.5478119100669332 1102 20-21 0.5830379671636692 1102 22-23 0.5965194007847714 1102 24-25 0.5615704783663062 1102 26-27 0.553985269682002 1102 28-29 0.5921409376978986 1102 30-31 0.5681354887763561 1102 32-33 0.5466763515043027 1102 34-35 0.5357545446778929 1102 36-37 0.5590629926203619 1102 38-39 0.5943144770285045 1102 40-41 0.5387218990139715 1102 42-43 0.5792848604396639 1102 44-45 0.6137525253753751 1102 46-47 0.6396589404672142 1102 48-49 0.6501340189029037 1102 50-51 0.5967966265611508 1102 52-53 0.6074911219668024 1102 54-55 0.6715848634988362 1102 56-57 0.6804872406081799 1102 58-59 0.6007580237400418 1102 60-61 0.5984519781731237 1102 62-63 0.6007293674513292 1102 64-65 0.5760582164993409 1102 66-67 0.550899853869403 1102 68-69 0.5394729526588975 1102 70-71 0.5553450746931148 1102 72-73 0.49371555643528353 1102 74-75 0.4698579418078097 1102 76-77 0.4900533645885474 1102 78-79 0.5161394138126241 1102 80-81 0.48336255281152063 1102 82-83 0.4705890535447175 1102 84-85 0.4477654304369558 1102 86-87 0.40154908467759043 1102 88-89 0.36494817995423645 1102 90-91 0.3864113273565337 1102 92-93 0.39046321077685775 1102 94-95 0.45442654328867604 1102 96-97 0.39363947587959913 1102 98-99 0.3370481697965815 1102 100 0.27848544487783755 1103 1 0.4081674370946402 1103 2 0.43842962066975133 1103 3 0.45656650528451337 1103 4 0.4132417760888387 1103 5 0.4047398398411346 1103 6 0.3992110320186555 1103 7 0.38519686859390845 1103 8 0.4268548409234114 1103 9 0.4955304704290526 1103 10-11 0.46000150959515196 1103 12-13 0.47652200774012243 1103 14-15 0.5556987108540312 1103 16-17 0.5015745307927872 1103 18-19 0.49998450871938616 1103 20-21 0.5285541747903864 1103 22-23 0.5714163873700926 1103 24-25 0.5959011020441523 1103 26-27 0.5413113376666558 1103 28-29 0.5192069278741585 1103 30-31 0.5268282361062475 1103 32-33 0.46553478952559857 1103 34-35 0.5033832829673486 1103 36-37 0.48229394219207933 1103 38-39 0.5081324003715011 1103 40-41 0.4955450910275303 1103 42-43 0.5260082194244049 1103 44-45 0.5073007457648373 1103 46-47 0.5519935792511745 1103 48-49 0.5955573058030623 1103 50-51 0.5122663275348316 1103 52-53 0.5091044281227539 1103 54-55 0.5717825314504736 1103 56-57 0.5844826213672931 1103 58-59 0.5229541259139197 1103 60-61 0.5761391538688727 1103 62-63 0.5277906587650278 1103 64-65 0.4609604126144973 1103 66-67 0.4408155903962978 1103 68-69 0.4663817179894636 1103 70-71 0.44023764697865175 1103 72-73 0.4573112729110633 1103 74-75 0.4743521489653588 1103 76-77 0.4577709640010852 1103 78-79 0.4144290475734067 1103 80-81 0.3644756876994819 1103 82-83 0.37784020751835 1103 84-85 0.36289974238727396 1103 86-87 0.32473564993641446 1103 88-89 0.33812919373099604 1103 90-91 0.3567861767123546 1103 92-93 0.30171675968221834 1103 94-95 0.35965156252585473 1103 96-97 0.3495660463967134 1103 98-99 0.2929437742749883 1103 100 0.3443744743196788 1104 1 0.33204766341776093 1104 2 0.3616030703413031 1104 3 0.36560307616542786 1104 4 0.3003517765937076 1104 5 0.33640218929169663 1104 6 0.23717698754575167 1104 7 0.2722762195278605 1104 8 0.32023565392087505 1104 9 0.3704746195034119 1104 10-11 0.333818982129543 1104 12-13 0.32387478927035573 1104 14-15 0.41548531476931316 1104 16-17 0.39286405501329824 1104 18-19 0.38941153857061295 1104 20-21 0.3429966958257964 1104 22-23 0.39287797837375393 1104 24-25 0.4321937746511537 1104 26-27 0.36541466173339643 1104 28-29 0.3712392746966984 1104 30-31 0.3230523113388344 1104 32-33 0.321120922273316 1104 34-35 0.3150441771575814 1104 36-37 0.29007280405794944 1104 38-39 0.29840622794758076 1104 40-41 0.2902973484830582 1104 42-43 0.3278985142657618 1104 44-45 0.31948793150513666 1104 46-47 0.36669375183655717 1104 48-49 0.38332404333666403 1104 50-51 0.3425150458505968 1104 52-53 0.3231474043575844 1104 54-55 0.4381252200624246 1104 56-57 0.45524083615777045 1104 58-59 0.3709712773432585 1104 60-61 0.41676353984225756 1104 62-63 0.4436271785699759 1104 64-65 0.37478323375589895 1104 66-67 0.43816342674056585 1104 68-69 0.36576027512637665 1104 70-71 0.3351521443376555 1104 72-73 0.33535987576912163 1104 74-75 0.35072907537945497 1104 76-77 0.3502848971718038 1104 78-79 0.3470184487754153 1104 80-81 0.2972715191676656 1104 82-83 0.3005472078343381 1104 84-85 0.29799133943895484 1104 86-87 0.23626557146308613 1104 88-89 0.2496136259113868 1104 90-91 0.24845585108063872 1104 92-93 0.23863296663855138 1104 94-95 0.2957300362701609 1104 96-97 0.2741632422515963 1104 98-99 0.26646508315721107 1104 100 0.20556403608035012 1105 1 0.26910201663655897 1105 2 0.31707719083489394 1105 3 0.293115835866125 1105 4 0.2710131903750792 1105 5 0.2829475602875391 1105 6 0.2701042306314463 1105 7 0.2669535955862017 1105 8 0.26859961681129363 1105 9 0.2955867293015402 1105 10-11 0.30320442584097407 1105 12-13 0.3009989069958934 1105 14-15 0.3626374352654267 1105 16-17 0.3225310457802024 1105 18-19 0.3535545345009936 1105 20-21 0.3555921972955858 1105 22-23 0.3976452613366277 1105 24-25 0.44020864522282466 1105 26-27 0.3996062195447365 1105 28-29 0.35604924666541393 1105 30-31 0.3951230199356246 1105 32-33 0.3770894432013776 1105 34-35 0.4055666300070939 1105 36-37 0.37432111057295714 1105 38-39 0.3944217363896598 1105 40-41 0.34717862721775106 1105 42-43 0.4067887866566764 1105 44-45 0.40921765975952695 1105 46-47 0.39509332169632927 1105 48-49 0.4198447877433793 1105 50-51 0.3008892678080102 1105 52-53 0.3646891467947313 1105 54-55 0.49068317198160116 1105 56-57 0.5017132387524796 1105 58-59 0.43487591109551715 1105 60-61 0.4293182225340644 1105 62-63 0.38826312908471294 1105 64-65 0.3673751825306155 1105 66-67 0.36721138762984395 1105 68-69 0.3597946963310079 1105 70-71 0.369423274767648 1105 72-73 0.3533080403769091 1105 74-75 0.36162301478380954 1105 76-77 0.36121627050461314 1105 78-79 0.3897027194104794 1105 80-81 0.32163756084998596 1105 82-83 0.2997113505852731 1105 84-85 0.32715519936690285 1105 86-87 0.28482197576458645 1105 88-89 0.295681365268031 1105 90-91 0.29981284764269134 1105 92-93 0.29591099092889195 1105 94-95 0.32607744913045167 1105 96-97 0.31461418737895386 1105 98-99 0.3132602732408998 1105 100 0.2638421365495027 1106 1 0.20865882307197126 1106 2 0.27127399219696713 1106 3 0.255594890186579 1106 4 0.24098384675640716 1106 5 0.24350993351105643 1106 6 0.20343765889298027 1106 7 0.21932467860850835 1106 8 0.23559718600648694 1106 9 0.24497946032562368 1106 10-11 0.21185250177618542 1106 12-13 0.23954847392083423 1106 14-15 0.3034690726913638 1106 16-17 0.27877655669895773 1106 18-19 0.2692589128516971 1106 20-21 0.2722978754119154 1106 22-23 0.2903472195535244 1106 24-25 0.25724346515158913 1106 26-27 0.2900211538265012 1106 28-29 0.3061664506733095 1106 30-31 0.2707422947196747 1106 32-33 0.28301533104755805 1106 34-35 0.2978004121161675 1106 36-37 0.2562209687101742 1106 38-39 0.2901820008889686 1106 40-41 0.32065638313252975 1106 42-43 0.3088001024142457 1106 44-45 0.31113979771300393 1106 46-47 0.3186304738812993 1106 48-49 0.31854499114185586 1106 50-51 0.19486753648149602 1106 52-53 0.259143465230423 1106 54-55 0.3718780729585802 1106 56-57 0.3795036829926417 1106 58-59 0.3182881555127466 1106 60-61 0.2959436119294523 1106 62-63 0.2545453867087204 1106 64-65 0.2584129577155494 1106 66-67 0.27429801842623647 1106 68-69 0.3064580094066258 1106 70-71 0.2755395996865744 1106 72-73 0.203348080888631 1106 74-75 0.22944043303960626 1106 76-77 0.21483941538335927 1106 78-79 0.2447647460164717 1106 80-81 0.247629357612972 1106 82-83 0.22612671477731183 1106 84-85 0.22996176431692916 1106 86-87 0.16508071051114115 1106 88-89 0.1751369594949317 1106 90-91 0.15299157202672475 1106 92-93 0.13169179704857115 1106 94-95 0.18797864841458534 1106 96-97 0.20644934090829636 1106 98-99 0.19443229130460793 1106 100 0.18171092829630453 1107 1 0.19709765829474435 1107 2 0.23268029115406108 1107 3 0.22942384316947795 1107 4 0.24139998158359788 1107 5 0.23919263125244328 1107 6 0.2073656682395537 1107 7 0.2077265998911173 1107 8 0.2404113569634987 1107 9 0.23118789385449645 1107 10-11 0.22471645886640346 1107 12-13 0.20865739973853437 1107 14-15 0.27628675289452076 1107 16-17 0.22764313325989605 1107 18-19 0.24189821115332677 1107 20-21 0.2397673339787474 1107 22-23 0.24789952073113142 1107 24-25 0.2906097418482503 1107 26-27 0.27006037900314794 1107 28-29 0.276718735675459 1107 30-31 0.2471757333615372 1107 32-33 0.20738481601372172 1107 34-35 0.314139677450207 1107 36-37 0.27465183162872364 1107 38-39 0.23703947649865142 1107 40-41 0.3093401650214602 1107 42-43 0.3325453854549565 1107 44-45 0.3261992481323901 1107 46-47 0.2974311773961702 1107 48-49 0.3945435418802532 1107 50-51 0.2857678350809252 1107 52-53 0.2847956098118516 1107 54-55 0.3930497644008071 1107 56-57 0.3857248557726436 1107 58-59 0.31570841080254297 1107 60-61 0.3401306018931436 1107 62-63 0.3195721359211845 1107 64-65 0.26725587009468654 1107 66-67 0.2935154626665053 1107 68-69 0.28970745684102894 1107 70-71 0.270503803184603 1107 72-73 0.2704939491628835 1107 74-75 0.3059658809229475 1107 76-77 0.2613099737103184 1107 78-79 0.29086245730756133 1107 80-81 0.2252865764642138 1107 82-83 0.251681928731589 1107 84-85 0.26276107650798863 1107 86-87 0.21110561957189589 1107 88-89 0.21869602618167505 1107 90-91 0.2735857487221125 1107 92-93 0.26005202929250615 1107 94-95 0.2410428552421493 1107 96-97 0.28523869715489525 1107 98-99 0.3061104569689945 1107 100 0.3088015800517603 1108 1 0.113667237666359 1108 2 0.1488741610422224 1108 3 0.16547754429669936 1108 4 0.15245668766010567 1108 5 0.1806473810551168 1108 6 0.10839448621577219 1108 7 0.12488538494716295 1108 8 0.13652532486702995 1108 9 0.1372265574539071 1108 10-11 0.12686534464392452 1108 12-13 0.11811767475317225 1108 14-15 0.16639833294699713 1108 16-17 0.12836548038489326 1108 18-19 0.14056595058760024 1108 20-21 0.11430965485316591 1108 22-23 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-1.1396973472308716 2316 82-83 -1.0870887436118117 2316 84-85 -1.1837954408576614 2316 86-87 -1.3263685368848215 2316 88-89 -1.2699678903342182 2316 90-91 -1.1853452449343287 2316 92-93 -1.0743446968114263 2316 94-95 -1.2775287840495082 2316 96-97 -1.449697083067246 2316 98-99 -1.3418237215815694 2316 100 -1.1417858693425806 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 12963.0 3 3168.0 4 2290.0 5 1953.0 6 2982.0 7 13861.0 8 26264.0 9 31097.0 10 32936.0 11 32505.0 12 33861.0 13 37438.0 14 44124.0 15 52511.0 16 65797.0 17 79531.0 18 88813.0 19 101051.0 20 119733.0 21 147151.0 22 188553.0 23 277788.0 24 403256.0 25 474472.0 26 458057.0 27 395702.0 28 359394.0 29 352613.0 30 389466.0 31 468329.0 32 580910.0 33 753373.0 34 1037550.0 35 1579033.0 36 2737729.0 37 5125144.0 38 6615719.0 39 2043662.0 40 4353.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 15.390540081460777 32.95032851603408 27.93607890223614 23.723052500269006 2 20.725160260883392 33.996221621689735 27.10663052838441 18.171987589042462 3 25.71277260091598 25.26052847957031 27.13263794348908 21.89406097602463 4 18.73626850685155 27.929858058728673 24.81533835091487 28.518535083504908 5 21.63610917522157 32.705466500210925 26.329038679053156 19.329385645514353 6 28.4432422618664 27.48598845708034 25.514402048586128 18.556367232467135 7 20.858968717429775 25.85876009297849 32.76877581280106 20.513495376790676 8 27.135245348819588 26.67863487913043 25.776241577404623 20.409878194645366 9 26.2439771821836 25.00003182046132 27.840000038184552 20.91599095917053 10-11 20.482831687258365 29.856851874810403 29.256054696519108 20.404261741412125 12-13 20.353240656209735 26.67592654335858 29.192406951494256 23.77842584893743 14-15 20.35683486661851 33.14415877218384 26.020993702446148 20.4780126587515 16-17 23.742214313812436 26.387057344267085 26.10167516736299 23.76905317455749 18-19 20.36590555659722 29.74167499434776 26.154233706339447 23.738185742715572 20-21 23.651410987885725 29.88930358976915 26.13864308679587 20.320642335549252 22-23 23.648211881769377 26.603937674184387 29.33393014642696 20.413920297619278 24-25 20.44787660200021 29.67653135638768 26.007366312485374 23.86822572912673 26-27 20.521368334004 26.425631850885157 32.63720972864828 20.415790086462565 28-29 20.460919482736585 26.563854478731475 32.62508827906147 20.350137759470464 30-31 20.3830854669296 26.457014518891004 32.66789642248972 20.49200359168968 32-33 20.51957287350262 26.611080160943345 32.44583415237345 20.42351281318058 34-35 20.544672131538658 26.73829754678802 32.30105050176661 20.415979819906713 36-37 20.436001809639677 26.668562775037437 32.39923421927161 20.49620119605127 38-39 20.45921363660763 26.661512249527338 32.43696508342959 20.44230903043545 40-41 20.480603928503093 26.706489357421383 32.3864425274989 20.42646418657662 42-43 20.462498722633402 26.745027849274756 32.34483011630386 20.447643311787985 44-45 20.50080154114674 26.81398692580282 32.30832879773916 20.376882735311284 46-47 20.471911556507504 26.91322653194255 32.25751743069995 20.35734448085 48-49 20.51634875591333 26.889805388357967 32.16990662914281 20.42393922658589 50-51 20.509474681582052 26.950844656334855 32.13003175804838 20.40964890403471 52-53 20.633652577603133 27.02780272567415 31.88954101344697 20.449003683275745 54-55 20.948243668725173 27.291744906307375 31.128582755890072 20.63142866907738 56-57 21.409343050815256 27.82746075582213 29.989041598443823 20.77415459491879 58-59 21.516739722960672 28.339040452449986 29.299174520541353 20.84504530404799 60-61 21.661311048745873 28.620463787379695 28.891552305836964 20.82667285803747 62-63 21.670288648727706 28.784861900426968 28.724381540150993 20.820467910694333 64-65 21.693302983407854 28.84698220002998 28.68668052531801 20.77303429124416 66-67 21.687078549475924 28.677766526315153 28.89487293563926 20.74028198856967 68-69 21.733855482690554 28.517145661204008 29.086195471039815 20.662803385065622 70-71 21.782087036481695 28.378559934327697 29.19259500250964 20.646758026680963 72-73 21.886334176028814 28.272748920596324 29.25077582014875 20.590141083226108 74-75 22.07826442916307 28.344534292304346 29.12081121236393 20.45639006616865 76-77 22.349008026425693 28.441197392743845 28.8185987867227 20.391195794107762 78-79 22.58207797778216 28.614483308304894 28.483225669885385 20.320213044027557 80-81 22.875972060873433 28.789213720480294 28.10014774390817 20.2346664747381 82-83 23.0189195520489 28.96239252947721 27.76398006050468 20.254707857969212 84-85 23.12189793251242 29.025914769272216 27.618452464535913 20.233734833679453 86-87 23.244803621206863 29.06104577987036 27.50426497989047 20.189885619032307 88-89 23.461562985159023 29.06960204644305 27.328310859125583 20.140524109272345 90-91 23.65778384964926 29.0526977503207 27.194209429921916 20.095308970108125 92-93 23.83592402075202 29.11320211661949 27.068283829437696 19.982590033190796 94-95 24.029765361141088 29.120793277333924 26.92634227778827 19.923099083736727 96-97 24.18630306911713 29.077616646986083 26.825940124913405 19.910140158983378 98-99 24.39199670851354 29.08811785716913 26.723250855931646 19.79663457838568 100 24.48694236116038 29.065686203705148 26.614650758588304 19.832720676546167 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 30812.0 1 22546.5 2 12129.5 3 9452.5 4 8745.5 5 8820.0 6 9138.5 7 9184.5 8 9145.5 9 9149.0 10 9662.0 11 12224.0 12 101556.5 13 126130.5 14 62308.0 15 65369.0 16 73816.0 17 82096.5 18 90296.5 19 98071.0 20 107916.5 21 123501.5 22 145282.0 23 169987.0 24 194346.5 25 222349.5 26 257075.0 27 298721.5 28 348906.5 29 398469.0 30 439729.5 31 479905.5 32 520855.5 33 554392.0 34 584528.5 35 620102.0 36 658287.0 37 697072.0 38 737944.0 39 762025.5 40 768787.0 41 788449.0 42 817905.0 43 821380.0 44 811711.5 45 818418.0 46 825734.0 47 823007.0 48 811256.5 49 795416.5 50 783566.5 51 772863.5 52 756956.5 53 723798.5 54 677076.0 55 632810.0 56 587637.0 57 535796.5 58 478776.0 59 415840.5 60 347065.5 61 283508.0 62 225551.5 63 171703.5 64 128932.5 65 95822.5 66 70581.5 67 53617.0 68 40884.0 69 31092.5 70 24233.0 71 19312.0 72 15932.0 73 13019.0 74 10552.5 75 8879.5 76 7138.0 77 5016.0 78 3508.5 79 2715.5 80 2129.0 81 1723.5 82 1377.5 83 1096.5 84 925.0 85 804.5 86 657.0 87 553.5 88 490.5 89 427.0 90 374.0 91 318.5 92 262.5 93 221.5 94 202.5 95 187.5 96 149.0 97 116.0 98 101.5 99 71.5 100 37.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4030564765261211 2 0.38390265441309307 3 0.16868630519990918 4 0.17491070156057176 5 0.2739171337810662 6 0.10204117301152581 7 0.13609859126061385 8 0.14204890762836914 9 0.13535579475809698 10-11 0.13188808702174828 12-13 0.13381657740662492 14-15 0.1398681842065416 16-17 0.1438363858429819 18-19 0.15524446902602138 20-21 0.13606085560941647 22-23 0.09882569831212802 24-25 0.09376713496477396 26-27 0.0882756046721026 28-29 0.0984066339751466 30-31 0.10314146515696522 32-33 0.10439865816790951 34-35 0.10481772250489094 36-37 0.10078993826129691 38-39 0.10002728088972881 40-41 0.10089321478036341 42-43 0.06335220009968567 44-45 0.06642666262881958 46-47 0.08464106563572338 48-49 0.0890680533472476 50-51 0.09856750701446174 52-53 0.10347909993083651 54-55 0.10606299899440447 56-57 0.10664293637070105 58-59 0.10612456768846336 60-61 0.10387631731186156 62-63 0.10843637284602917 64-65 0.11561607700805698 66-67 0.12409269591913162 68-69 0.12916516187482155 70-71 0.12755444539476496 72-73 0.11455749268762523 74-75 0.11192791362523938 76-77 0.11398549965894915 78-79 0.11282959708016624 80-81 0.10679983723620595 82-83 0.10985046672247836 84-85 0.11599144743312567 86-87 0.12939356186891093 88-89 0.13717902253700198 90-91 0.14403102235968415 92-93 0.14332993368217495 94-95 0.13330218089819748 96-97 0.13010855315475606 98-99 0.12503211502525588 100 0.11918110300275685 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2.5175132E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 39.82713602064435 #Duplication Level Percentage of deduplicated Percentage of total 1 67.48453915326239 26.87715920147481 2 16.736844489680568 13.331611640937599 3 6.241551808250656 7.4574939854129285 4 2.8906046502925014 4.604980183564263 5 1.5753875517960636 3.1371587155305867 6 0.9764814884924051 2.333427663829697 7 0.6978042719933304 1.9454081959525558 8 0.5004198312516075 1.5944230949348535 9 0.39537693177892796 1.4172057757245948 >10 2.16096273814017 15.958556821849198 >50 0.20379881205260414 5.762759900982358 >100 0.13465537027186053 8.906370844659158 >500 8.44957850614123E-4 0.20979508587441528 >1k 6.781776885167953E-4 0.5337184994268319 >5k 1.9906879029850657E-5 0.051990088164238696 >10k+ 2.9860318544563796E-5 5.877940301681849 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 1423071 5.652685356327029 No Hit AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 35906 0.14262487283085548 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0012115130121264112 2.383304286150317E-5 7.944347620501056E-6 3.972173810250528E-6 3.5749564292254755E-5 2 0.0012512347502289165 3.5749564292254755E-5 7.944347620501056E-6 3.972173810250528E-6 3.5749564292254755E-5 3 0.001330678226433927 4.766608572300634E-5 7.944347620501056E-6 7.944347620501056E-6 3.5749564292254755E-5 4 0.0013942330073979355 4.766608572300634E-5 7.944347620501056E-6 7.944347620501056E-6 3.5749564292254755E-5 5 0.0014021773550184364 5.163825953325687E-5 7.944347620501056E-6 7.944347620501056E-6 4.766608572300634E-5 6 0.0014339547455004408 5.56104333435074E-5 7.944347620501056E-6 1.1916521430751584E-5 4.766608572300634E-5 7 0.0014577877883619438 5.958260715375792E-5 7.944347620501056E-6 1.1916521430751584E-5 5.163825953325687E-5 8 0.001489565178843948 6.752695477425898E-5 7.944347620501056E-6 1.1916521430751584E-5 5.163825953325687E-5 9 0.0015213425693259522 6.752695477425898E-5 7.944347620501056E-6 1.1916521430751584E-5 5.163825953325687E-5 10-11 0.0015987999586258377 7.944347620501057E-5 1.1916521430751584E-5 1.5888695241002113E-5 5.56104333435074E-5 12-13 0.0016961182169769756 1.0327651906651373E-4 1.1916521430751584E-5 1.7874782146127377E-5 5.958260715375792E-5 14-15 0.0017715895193717356 1.112208666870148E-4 1.1916521430751584E-5 2.78052166717537E-5 6.554086786913371E-5 16-17 0.001827199952715243 1.1320695359214005E-4 1.1916521430751584E-5 2.78052166717537E-5 7.745738929988531E-5 18-19 0.0019185599503510052 1.1916521430751584E-4 1.1916521430751584E-5 2.979130357687896E-5 8.341565001526109E-5 20-21 0.002007933861081642 1.3505390954851793E-4 1.1916521430751584E-5 3.5749564292254755E-5 9.135999763576215E-5 22-23 0.002150932118250661 1.4299825716901902E-4 1.1916521430751584E-5 3.773565119738002E-5 9.533217144601268E-5 24-25 0.002417067763537446 1.5491477859977062E-4 1.1916521430751584E-5 3.9721738102505285E-5 9.533217144601268E-5 26-27 0.0033346399137053184 1.7477564765102323E-4 1.1916521430751584E-5 4.369391191275581E-5 9.731825835113795E-5 28-29 0.005318740731925457 1.886782559869001E-4 1.1916521430751584E-5 5.56104333435074E-5 1.112208666870148E-4 30-31 0.01008137713041584 1.986086905125264E-4 1.1916521430751584E-5 5.56104333435074E-5 1.112208666870148E-4 32-33 0.01928291776186119 2.1251129884840324E-4 1.1916521430751584E-5 6.355478096400845E-5 1.1717912740239058E-4 34-35 0.03561252429580111 2.1846955956377905E-4 1.1916521430751584E-5 6.554086786913371E-5 1.2512347502289164E-4 36-37 0.061797094052972595 2.264139071842801E-4 1.1916521430751584E-5 6.752695477425898E-5 1.271095619280169E-4 38-39 0.09851189658111822 2.3237216789965588E-4 1.1916521430751584E-5 7.348521548963477E-5 1.2909564883314216E-4 40-41 0.1485056761569314 2.3833042861503168E-4 1.1916521430751584E-5 8.142956311013583E-5 1.4101217026389373E-4 42-43 0.21156790756846877 2.4627477623553277E-4 1.3902608335876848E-5 8.341565001526109E-5 1.5094260478952005E-4 44-45 0.2902705733578676 2.542191238560338E-4 1.5888695241002113E-5 8.540173692038634E-5 1.608730393151464E-4 46-47 0.3912333011799104 2.562052107611591E-4 1.5888695241002113E-5 9.135999763576215E-5 1.6881738693564746E-4 48-49 0.5172385987886776 2.7407999290728644E-4 1.5888695241002113E-5 9.731825835113795E-5 1.7278956074589797E-4 50-51 0.6659389114623113 2.8599651433803804E-4 1.5888695241002113E-5 9.93043452562632E-5 1.8470608217664958E-4 52-53 0.8321505523784344 2.9791303576878964E-4 1.5888695241002113E-5 9.93043452562632E-5 1.886782559869001E-4 54-55 1.0206143109795809 3.058573833892907E-4 1.5888695241002113E-5 1.0327651906651373E-4 1.926504297971506E-4 56-57 1.24011067747331 3.138017310097917E-4 1.5888695241002113E-5 1.0923477978188952E-4 2.045669512279022E-4 58-59 1.4957577978141288 3.296904262507939E-4 1.5888695241002113E-5 1.112208666870148E-4 2.2442782027915485E-4 60-61 1.7817503399783563 3.634539036379233E-4 1.5888695241002113E-5 1.1320695359214005E-4 2.3833042861503168E-4 62-63 2.093009879749588 3.694121643532991E-4 1.5888695241002113E-5 1.1519304049726531E-4 2.4627477623553277E-4 64-65 2.4279276867346713 3.872869464994265E-4 1.5888695241002113E-5 1.1916521430751584E-4 2.4627477623553277E-4 66-67 2.7982296180214665 3.9125912030967707E-4 1.5888695241002113E-5 1.1916521430751584E-4 2.502469500457833E-4 68-69 3.20624336746278 4.0317564174042857E-4 1.5888695241002113E-5 1.1916521430751584E-4 2.562052107611591E-4 70-71 3.649903404677282 4.329669453173076E-4 1.5888695241002113E-5 1.1916521430751584E-4 2.581912976662843E-4 72-73 4.120262408157383 4.50841727463435E-4 1.5888695241002113E-5 1.1916521430751584E-4 2.581912976662843E-4 74-75 4.620903278679929 4.6474433579931176E-4 1.5888695241002113E-5 1.1916521430751584E-4 2.641495583816601E-4 76-77 5.152705852743891 4.6871650960956233E-4 1.5888695241002113E-5 1.1916521430751584E-4 2.7209390600216115E-4 78-79 5.719407945904712 4.6871650960956233E-4 1.5888695241002113E-5 1.1916521430751584E-4 2.820243405277875E-4 80-81 6.318240953016652 4.6871650960956233E-4 1.5888695241002113E-5 1.2115130121264111E-4 2.8401042743291275E-4 82-83 6.9428791872868825 4.7666085723006336E-4 1.5888695241002113E-5 1.2313738811776638E-4 2.8599651433803804E-4 84-85 7.595394137357452 4.865912917556897E-4 1.5888695241002113E-5 1.2512347502289164E-4 2.8996868814828856E-4 86-87 8.278659670980076 4.965217262813161E-4 1.5888695241002113E-5 1.3108173573826742E-4 2.979130357687896E-4 88 8.809344078116453 5.04466073901817E-4 1.5888695241002113E-5 1.3108173573826742E-4 3.0188520957904016E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 184775 0.0 82.67656 8 AGTGGTA 185010 0.0 82.645744 5 AAGCAGT 186925 0.0 82.36663 1 TGGTATC 186200 0.0 81.782684 7 GTGGTAT 188170 0.0 81.252754 6 GTATCAA 188020 0.0 81.140785 9 GCAGTGG 190160 0.0 81.005325 3 AGCAGTG 195700 0.0 78.775696 2 CAGTGGT 194890 0.0 78.42193 4 CAACGCA 182960 0.0 43.130054 12-13 GAGTACT 174535 0.0 42.89699 20-21 ACGCAGA 184265 0.0 42.857944 14-15 ATCAACG 184610 0.0 42.856323 10-11 AGAGTAC 183300 0.0 42.44093 18-19 AGTACTT 182000 0.0 42.390163 20-21 TCAACGC 182930 0.0 41.76359 12-13 AACGCAG 183475 0.0 41.761265 14-15 CGCAGAG 185025 0.0 41.329216 16-17 GTACTTT 183110 0.0 40.90643 22-23 TATCAAC 188280 0.0 40.429535 10-11 >>END_MODULE