FastQCFastQC Report
Thu 26 May 2016
SRR1660722_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660722_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25175132
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT14974965.948314392154925No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT382070.15176484476824192No Hit
TAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT328200.13036674445242233No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1922500.084.325621
GGTATCA2026050.083.031928
AGTGGTA2015050.082.780685
GCAGTGG2014700.082.752013
TGGTATC2039250.082.429657
GTGGTAT2043650.081.592336
GTATCAA2061600.081.4908459
AGCAGTG2056900.080.679022
CAGTGGT2107350.079.006324
CAACGCA2005200.043.2936612-13
ATCAACG2018950.043.0801810-11
ACGCAGA2024800.042.9825914-15
GAGTACT1944200.042.92159720-21
AGTACTT2019400.042.69705220-21
AGAGTAC2036500.042.6219318-19
TCAACGC2005750.041.8366812-13
AACGCAG2013100.041.79256414-15
CGCAGAG2031950.041.39702216-17
GTACTTT2036000.041.0002622-23
TATCAAC2063600.040.66157510-11
Produced by FastQC (version 0.11.5)