##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660722_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 25175132 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.19220916100857 34.0 31.0 34.0 27.0 34.0 2 31.847353253202407 34.0 31.0 34.0 28.0 34.0 3 31.62404038239005 34.0 31.0 34.0 30.0 34.0 4 35.553438210373635 37.0 35.0 37.0 33.0 37.0 5 35.89116231843392 37.0 35.0 37.0 33.0 37.0 6 35.35195648626589 37.0 35.0 37.0 33.0 37.0 7 35.55477532352164 37.0 35.0 37.0 33.0 37.0 8 35.92916720357216 37.0 35.0 37.0 33.0 37.0 9 37.73631923757143 39.0 37.0 39.0 35.0 39.0 10-11 37.744538876697845 39.0 37.5 39.0 35.0 39.0 12-13 37.8317732355882 39.0 38.0 39.0 35.0 39.0 14-15 39.27992619859947 41.0 39.0 41.0 36.0 41.0 16-17 39.246309393730286 41.0 39.0 41.0 36.0 41.0 18-19 39.241469637577275 41.0 39.0 41.0 36.0 41.0 20-21 39.1712726272895 40.5 39.0 41.0 36.0 41.0 22-23 39.03673353132766 40.0 38.5 41.0 35.5 41.0 24-25 39.058274411431086 40.0 39.0 41.0 36.0 41.0 26-27 39.02390148341625 40.0 39.0 41.0 35.0 41.0 28-29 38.94748178480256 40.0 38.5 41.0 35.0 41.0 30-31 38.8526889551165 40.0 38.0 41.0 35.0 41.0 32-33 38.731010784769666 40.0 38.0 41.0 35.0 41.0 34-35 38.64481959816536 40.0 38.0 41.0 34.5 41.0 36-37 38.508821880258665 40.0 38.0 41.0 34.0 41.0 38-39 38.07696201950401 40.0 37.0 41.0 34.0 41.0 40-41 37.944930239094674 40.0 37.0 41.0 33.0 41.0 42-43 37.778168313079746 40.0 36.0 41.0 33.0 41.0 44-45 37.65215342267123 40.0 36.0 41.0 33.0 41.0 46-47 37.496947642618125 40.0 36.0 41.0 33.0 41.0 48-49 37.53541840416169 40.0 35.5 41.0 33.0 41.0 50-51 37.52485005838301 40.0 36.0 41.0 33.0 41.0 52-53 37.24975882946711 40.0 35.0 41.0 32.5 41.0 54-55 36.67489338288276 39.5 35.0 41.0 30.5 41.0 56-57 36.12766157492243 39.0 35.0 41.0 27.5 41.0 58-59 35.964998177566656 39.0 35.0 41.0 27.5 41.0 60-61 35.81868728632684 39.0 35.0 41.0 27.0 41.0 62-63 35.590060461252 39.0 35.0 41.0 27.0 41.0 64-65 35.336020839930455 38.0 35.0 40.0 26.5 41.0 66-67 35.067260779407235 37.0 35.0 40.0 26.0 41.0 68-69 34.81268495434304 37.0 34.5 40.0 26.0 41.0 70-71 34.52217763942608 36.0 34.0 39.0 26.0 41.0 72-73 34.18966496382224 36.0 34.0 39.0 26.0 41.0 74-75 33.8194920487408 35.0 34.0 38.5 26.0 40.0 76-77 32.11831604299036 34.5 31.5 36.5 23.0 39.0 78-79 33.06053952368552 35.0 33.5 37.0 25.0 39.0 80-81 32.96765844167172 35.0 34.0 36.5 25.0 39.0 82-83 32.71160224701106 35.0 34.0 36.0 25.0 38.0 84-85 32.46456940126471 35.0 33.5 36.0 24.0 37.0 86-87 32.25517482887478 35.0 33.0 35.5 24.0 37.0 88-89 32.08449318160477 35.0 33.0 35.0 24.0 36.5 90-91 31.92603446925323 35.0 33.0 35.0 23.0 36.0 92-93 31.787697220415765 35.0 33.0 35.0 23.0 36.0 94-95 31.689160120391822 35.0 33.0 35.0 20.5 36.0 96-97 31.59445092482534 35.0 33.0 35.0 20.0 35.0 98-99 31.50346931646674 35.0 33.0 35.0 20.0 35.0 100 31.449725506901018 35.0 33.0 35.0 20.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.620945544633873 1101 2 1.2102271286922601 1101 3 1.553843590832603 1101 4 0.9713494111087755 1101 5 0.5787614358315807 1101 6 1.1156693900768104 1101 7 0.8329781519053654 1101 8 0.4903850036346 1101 9 0.5153210023503618 1101 10-11 0.432964898825567 1101 12-13 0.3928791411257677 1101 14-15 0.5190047010930741 1101 16-17 0.5491463146217157 1101 18-19 0.5202632405190357 1101 20-21 0.5211352736502377 1101 22-23 0.5088407795815897 1101 24-25 0.5093069715310676 1101 26-27 0.4799101823758818 1101 28-29 0.4926773388882424 1101 30-31 0.5101492926995022 1101 32-33 0.5270967002193885 1101 34-35 0.5165227236634635 1101 36-37 0.4879343130557672 1101 38-39 0.5347420888833625 1101 40-41 0.5307834718533897 1101 42-43 0.5360115484681529 1101 44-45 0.49271444002490483 1101 46-47 0.49893339103066126 1101 48-49 0.4692356300451763 1101 50-51 0.43600361949689415 1101 52-53 0.457521888302999 1101 54-55 0.49664214545591534 1101 56-57 0.556480782546565 1101 58-59 0.5172081368276338 1101 60-61 0.5074386270973648 1101 62-63 0.4717084712517874 1101 64-65 0.4583483931329795 1101 66-67 0.472048704381379 1101 68-69 0.4758535797067154 1101 70-71 0.47381289325701914 1101 72-73 0.45100230286620757 1101 74-75 0.5092175238109107 1101 76-77 0.39080610095099644 1101 78-79 0.4990451422790798 1101 80-81 0.5357005221537676 1101 82-83 0.5069932621985558 1101 84-85 0.49440502181360557 1101 86-87 0.4403463149292435 1101 88-89 0.4551327889939216 1101 90-91 0.47985182556681494 1101 92-93 0.46282083116426165 1101 94-95 0.44687129888159305 1101 96-97 0.4574121058347238 1101 98-99 0.4505059201603494 1101 100 0.4215529352919951 1102 1 1.5115413646838967 1102 2 1.1401412862203486 1102 3 1.471578943001166 1102 4 0.9095599770851237 1102 5 0.49383733373021244 1102 6 1.0656128399173994 1102 7 0.7822151212744686 1102 8 0.4452994792707017 1102 9 0.464442361287837 1102 10-11 0.3635479697836459 1102 12-13 0.3469578347393778 1102 14-15 0.45027498560245505 1102 16-17 0.4820320754762051 1102 18-19 0.44054463966892143 1102 20-21 0.3889317377540422 1102 22-23 0.4349009716589478 1102 24-25 0.41778698443929585 1102 26-27 0.4450150031540048 1102 28-29 0.4377662443749841 1102 30-31 0.4170591217844333 1102 32-33 0.4579530923441695 1102 34-35 0.4284330169009536 1102 36-37 0.4291798990175053 1102 38-39 0.4438969764053553 1102 40-41 0.44551033308643895 1102 42-43 0.46966794380431054 1102 44-45 0.4311194773695348 1102 46-47 0.45640276854550166 1102 48-49 0.4298209423727144 1102 50-51 0.3861695956939286 1102 52-53 0.4065671441309888 1102 54-55 0.44072987057480617 1102 56-57 0.4569700914798034 1102 58-59 0.4289375495720904 1102 60-61 0.46621498524215355 1102 62-63 0.4470379050680009 1102 64-65 0.4208587245149218 1102 66-67 0.39813814323812835 1102 68-69 0.3684981980248594 1102 70-71 0.38283825423565787 1102 72-73 0.333409249678688 1102 74-75 0.3660576306588439 1102 76-77 0.20968784890408898 1102 78-79 0.3996122050083457 1102 80-81 0.4155233693163538 1102 82-83 0.4011219925095375 1102 84-85 0.3857724208676814 1102 86-87 0.3863755882443627 1102 88-89 0.3716500035560486 1102 90-91 0.40667283488486916 1102 92-93 0.3886750059781754 1102 94-95 0.382684898706632 1102 96-97 0.42521096064944075 1102 98-99 0.40801110933072593 1102 100 0.4050201846731376 1103 1 1.4914743109696325 1103 2 1.0983808913600015 1103 3 1.43003348307629 1103 4 0.8983841661948517 1103 5 0.5021025507540671 1103 6 1.0423652153445246 1103 7 0.7653078039170964 1103 8 0.43574382632485964 1103 9 0.4610229007499811 1103 10-11 0.37080977410131766 1103 12-13 0.3353439381629144 1103 14-15 0.40764395276344345 1103 16-17 0.4283283497863053 1103 18-19 0.3777267608972963 1103 20-21 0.4202737553472886 1103 22-23 0.3884792373073793 1103 24-25 0.4001309549258778 1103 26-27 0.3989213382065486 1103 28-29 0.3701476635217489 1103 30-31 0.39415546381735567 1103 32-33 0.44396575424536877 1103 34-35 0.43358888187196953 1103 36-37 0.370750215226451 1103 38-39 0.3945997149394529 1103 40-41 0.41903441110650164 1103 42-43 0.4560373842143406 1103 44-45 0.4195600103121464 1103 46-47 0.4142282571312137 1103 48-49 0.39545514711250007 1103 50-51 0.35667189398859733 1103 52-53 0.3217018425144502 1103 54-55 0.35397217503217604 1103 56-57 0.3734544959960786 1103 58-59 0.3940626265925289 1103 60-61 0.35479923965875315 1103 62-63 0.30236106557903497 1103 64-65 0.298345550248591 1103 66-67 0.2957415251825921 1103 68-69 0.2745283075468308 1103 70-71 0.26286064047198465 1103 72-73 0.26717152188056303 1103 74-75 0.2916009858715043 1103 76-77 0.14819012440742796 1103 78-79 0.2881563155991316 1103 80-81 0.3250499012361985 1103 82-83 0.32387250702265646 1103 84-85 0.31199849861101114 1103 86-87 0.2901255942361374 1103 88-89 0.2939477170911715 1103 90-91 0.2658153484592951 1103 92-93 0.28859076273113615 1103 94-95 0.2760787143901453 1103 96-97 0.3140762958445471 1103 98-99 0.2996894784125068 1103 100 0.31622743971035305 1104 1 1.406931223147975 1104 2 1.0462328318635485 1104 3 1.3945532269210297 1104 4 0.8728068232103681 1104 5 0.4589132642184097 1104 6 1.0135092002653963 1104 7 0.7551325297794165 1104 8 0.4047372112932237 1104 9 0.3974994297579215 1104 10-11 0.3028723067254404 1104 12-13 0.2961771662637531 1104 14-15 0.3414665525255387 1104 16-17 0.37652800227215977 1104 18-19 0.34124817375954564 1104 20-21 0.3433650331894782 1104 22-23 0.35407000805521704 1104 24-25 0.32656465589354156 1104 26-27 0.32003423685571164 1104 28-29 0.2967171040545935 1104 30-31 0.3126155566037383 1104 32-33 0.35501769736222144 1104 34-35 0.35669698937284977 1104 36-37 0.35263625746859617 1104 38-39 0.4056684141008091 1104 40-41 0.3652839683478746 1104 42-43 0.3959598141147751 1104 44-45 0.3799898307849432 1104 46-47 0.37215577056626614 1104 48-49 0.32572322672464793 1104 50-51 0.3368298260822087 1104 52-53 0.3720664144446175 1104 54-55 0.3613316243201936 1104 56-57 0.42488664033054135 1104 58-59 0.38353984367835636 1104 60-61 0.36273619602952323 1104 62-63 0.34737185450404695 1104 64-65 0.32396231707758005 1104 66-67 0.3362966202746236 1104 68-69 0.27522782246618505 1104 70-71 0.30097814574321546 1104 72-73 0.32099951257781356 1104 74-75 0.32277099562906386 1104 76-77 0.19135388369224415 1104 78-79 0.32500188476465297 1104 80-81 0.33779082511379954 1104 82-83 0.3603184987979873 1104 84-85 0.3145594171061816 1104 86-87 0.31345514162793364 1104 88-89 0.3117369136212176 1104 90-91 0.3251781191118077 1104 92-93 0.3094669105065826 1104 94-95 0.29552115698043835 1104 96-97 0.31438560959659156 1104 98-99 0.332872228315626 1104 100 0.3579912029703358 1105 1 1.3594461111847416 1105 2 1.0001513650616118 1105 3 1.3256973785572406 1105 4 0.8221289671552867 1105 5 0.41738974982277455 1105 6 0.9649837180866001 1105 7 0.701572347197839 1105 8 0.3374682520239176 1105 9 0.296648035154071 1105 10-11 0.23360567194870896 1105 12-13 0.20131880892771647 1105 14-15 0.26584018844908996 1105 16-17 0.26532678013875 1105 18-19 0.24552778121385188 1105 20-21 0.26190879054696126 1105 22-23 0.27494790929033286 1105 24-25 0.23481972613306823 1105 26-27 0.248003745285871 1105 28-29 0.276565866255595 1105 30-31 0.2682441963736295 1105 32-33 0.28452228161616233 1105 34-35 0.3093355856005999 1105 36-37 0.313137098000162 1105 38-39 0.32770742976049405 1105 40-41 0.33277720774673725 1105 42-43 0.302651667715395 1105 44-45 0.2766021308223614 1105 46-47 0.2645365215653044 1105 48-49 0.2641690056188537 1105 50-51 0.2391093116246239 1105 52-53 0.2652600379526149 1105 54-55 0.2720606721231249 1105 56-57 0.28539077509079647 1105 58-59 0.2795085638289976 1105 60-61 0.23065824106090105 1105 62-63 0.20035470497229824 1105 64-65 0.21469331928187074 1105 66-67 0.17029745309804412 1105 68-69 0.1430093783330122 1105 70-71 0.14671426798911114 1105 72-73 0.19880079076289547 1105 74-75 0.21585901720464307 1105 76-77 0.09745293552175127 1105 78-79 0.19490246747168527 1105 80-81 0.20697629478076607 1105 82-83 0.22911118801874153 1105 84-85 0.19547001269241804 1105 86-87 0.2447430777319468 1105 88-89 0.23595776925730405 1105 90-91 0.21636986022796734 1105 92-93 0.17693861294963753 1105 94-95 0.19350210211868202 1105 96-97 0.169681591011539 1105 98-99 0.19419161930690976 1105 100 0.15678888436059069 1106 1 1.3093685701638371 1106 2 0.9647983555568374 1106 3 1.3124659245218133 1106 4 0.776240392048976 1106 5 0.39796033951940046 1106 6 0.9298748625592239 1106 7 0.6519525199855423 1106 8 0.3142490960037918 1106 9 0.2881599075089838 1106 10-11 0.20084679340445177 1106 12-13 0.17486311640973184 1106 14-15 0.19718288657028182 1106 16-17 0.21605760489617154 1106 18-19 0.18325428868734406 1106 20-21 0.20995816555030444 1106 22-23 0.2188518532246917 1106 24-25 0.2311072618994956 1106 26-27 0.20098665063963495 1106 28-29 0.21963507238215385 1106 30-31 0.22560523816386535 1106 32-33 0.2719082117956759 1106 34-35 0.28255995055309313 1106 36-37 0.2385844260075416 1106 38-39 0.24982595156701848 1106 40-41 0.2719261115887832 1106 42-43 0.27153558474555695 1106 44-45 0.25049246168291717 1106 46-47 0.2537536402377967 1106 48-49 0.23873791151142143 1106 50-51 0.2551251022637615 1106 52-53 0.19855235590875964 1106 54-55 0.2312955729677455 1106 56-57 0.25644292368399846 1106 58-59 0.2446587856985829 1106 60-61 0.206413591208225 1106 62-63 0.17166730186701784 1106 64-65 0.14205199540757718 1106 66-67 0.14857979522834341 1106 68-69 0.13710371591551507 1106 70-71 0.13077844930697324 1106 72-73 0.06768581124919848 1106 74-75 0.12351645259894184 1106 76-77 0.002499708310935489 1106 78-79 0.1742609629443237 1106 80-81 0.19365626213340903 1106 82-83 0.16883539124152236 1106 84-85 0.18705966305211064 1106 86-87 0.18985775760917534 1106 88-89 0.17476681752897605 1106 90-91 0.1591117912949933 1106 92-93 0.1807838260200647 1106 94-95 0.19239608594755708 1106 96-97 0.18841580122490953 1106 98-99 0.20498573717069846 1106 100 0.1864936627494842 1107 1 1.3055938060157608 1107 2 0.9146768497720004 1107 3 1.279687206531566 1107 4 0.783433888135626 1107 5 0.38181027941433854 1107 6 0.936173804206824 1107 7 0.6340140510617189 1107 8 0.2985524835737863 1107 9 0.28103309522857245 1107 10-11 0.2012702086911844 1107 12-13 0.1319895426326454 1107 14-15 0.1858707458318989 1107 16-17 0.19440028748792315 1107 18-19 0.15543211937727364 1107 20-21 0.14695269658735555 1107 22-23 0.1691399743955273 1107 24-25 0.18150021380416348 1107 26-27 0.1468004165981185 1107 28-29 0.1519945806448888 1107 30-31 0.17504805265168955 1107 32-33 0.19809237514992617 1107 34-35 0.21200887299565352 1107 36-37 0.1914573294778137 1107 38-39 0.195899514437059 1107 40-41 0.19748501472968627 1107 42-43 0.21758596051478207 1107 44-45 0.17489271693779784 1107 46-47 0.23963306637070758 1107 48-49 0.2070917539478927 1107 50-51 0.1525646214839398 1107 52-53 0.1547892731653917 1107 54-55 0.21567789144078375 1107 56-57 0.20697909212167787 1107 58-59 0.13681684907761138 1107 60-61 0.1305591990872017 1107 62-63 0.10562574567879324 1107 64-65 0.061177517913783674 1107 66-67 0.11212810865663414 1107 68-69 0.09439595687096158 1107 70-71 0.1268722371911366 1107 72-73 0.12097503985558689 1107 74-75 0.14352467457523943 1107 76-77 -0.01501680457148069 1107 78-79 0.11496407808211728 1107 80-81 0.16951311991390838 1107 82-83 0.14046265205895025 1107 84-85 0.15321647250186743 1107 86-87 0.09652069747941994 1107 88-89 0.13551416433992358 1107 90-91 0.15102264889744887 1107 92-93 0.1570876425246226 1107 94-95 0.11531498566623455 1107 96-97 0.1385184027269588 1107 98-99 0.17067451595237415 1107 100 0.17611506376428565 1108 1 1.24636023335891 1108 2 0.8834046490094494 1108 3 1.2124064354339161 1108 4 0.7400701747486025 1108 5 0.3275156502562879 1108 6 0.8592319266432042 1108 7 0.597091596967843 1108 8 0.24893921990693713 1108 9 0.19910864978309917 1108 10-11 0.11316815422130588 1108 12-13 0.10559687693100983 1108 14-15 0.12779848190312038 1108 16-17 0.11460558265790155 1108 18-19 0.08312551121662182 1108 20-21 0.11870506505086098 1108 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-0.7752891513799973 2316 94-95 -0.784583768802019 2316 96-97 -0.8936103314208772 2316 98-99 -1.0449899690896594 2316 100 -0.962733603536126 >>END_MODULE >>Per sequence quality scores pass #Quality Count 5 1.0 6 51.0 7 714.0 8 888.0 9 353.0 10 355.0 11 672.0 12 881.0 13 1247.0 14 2680.0 15 6302.0 16 13197.0 17 20487.0 18 28557.0 19 38826.0 20 55885.0 21 83454.0 22 125726.0 23 212473.0 24 366807.0 25 470502.0 26 460841.0 27 381470.0 28 326104.0 29 332405.0 30 378145.0 31 457960.0 32 564541.0 33 722912.0 34 990202.0 35 1534996.0 36 2768832.0 37 5505426.0 38 7215410.0 39 2101137.0 40 4693.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 16.46117364282066 32.91110879433465 28.12357924746425 22.50413831538044 2 21.000371500478995 34.597316838584945 26.433156846771304 17.96915481416475 3 26.275147212001354 25.2277591809236 27.18608626575462 21.31100734132043 4 19.184758384704903 27.842161306827744 24.65496509879258 28.318115209674772 5 21.48445369873113 33.13946467782477 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20.3750466747702 26.28280342572952 33.096316864091015 20.24583303540927 28-29 20.349546345624486 26.38682214992269 33.07379468210527 20.18983682234755 30-31 20.28977375332659 26.262986086178756 33.10081992799303 20.34642023250162 32-33 20.42943597582414 26.424253232639522 32.87136073546834 20.27495005606799 34-35 20.409143275784395 26.54349770447605 32.783077758571025 20.26428126116853 36-37 20.318204717175327 26.484482235772646 32.87199180226866 20.325321244783364 38-39 20.341701244689656 26.478307061583408 32.88138495086845 20.29860674285848 40-41 20.36518800575808 26.517181379287685 32.839629350185376 20.27800126476886 42-43 20.349130294011204 26.538007577298444 32.8105766965624 20.30228543212796 44-45 20.37837120345393 26.60544276033238 32.769501920541 20.24668411567269 46-47 20.412461287251197 26.69485171202316 32.66455803989816 20.22812896082748 48-49 20.40850683396544 26.699530515312553 32.59017331903095 20.301789331691054 50-51 20.432917260356255 26.77340982470645 32.50724075622998 20.286432158707306 52-53 20.552124677683857 26.864156294689263 32.24179528061738 20.341923747009506 54-55 20.85881978264623 27.120188677108597 31.48763023355567 20.533361306689507 56-57 21.350866294247787 27.65562401934913 30.224160519943418 20.76934916645967 58-59 21.448380597201115 28.256381460420698 29.415180650786837 20.88005729159135 60-61 21.5053772411909 28.573187711706776 29.000251861553654 20.921183185548664 62-63 21.498547727371335 28.77745241057708 28.780887214931106 20.943112647120476 64-65 21.46427600364046 28.86302497051126 28.70458930714952 20.968109718698756 66-67 21.336440385384403 28.72820861824441 28.946630867015553 20.98872012935563 68-69 21.26233312660967 28.58372099816333 29.136579298926595 21.017366576300407 70-71 21.199773932629277 28.464426911285884 29.266221237077556 21.069577919007283 72-73 21.17360257160464 28.370340498406698 29.31536835705287 21.14068857293579 74-75 21.26391698056544 28.439763609013784 29.196598386951187 21.099721023469584 76-77 21.342903322970994 28.55900815178969 29.000386145878405 21.09770237936091 78-79 21.444454060073525 28.782384812243027 28.63063561284351 21.142525514839935 80-81 21.55302785076087 29.00686509350665 28.220444053805906 21.219663001926577 82-83 21.550165341378978 29.208529238658688 27.840517599388626 21.400787820573704 84-85 21.51272406970092 29.301724845913473 27.644085908327355 21.541465176058253 86-87 21.510244200311412 29.334549416808336 27.49706400536856 21.658142377511687 88-89 21.50582907932259 29.378659949204067 27.341707187608264 21.773803783865073 90-91 21.5410683392628 29.386100413876363 27.185750404939533 21.887080841921293 92-93 21.542639060868552 29.43294123361345 27.059405923671857 21.96501378184614 94-95 21.581193514954165 29.443260380536152 26.90859466094394 22.066951443565745 96-97 21.57800160241708 29.381671802149988 26.796001398577157 22.244325196855776 98-99 21.578741418303693 29.406799125461735 26.69448146772463 22.319977988509937 100 21.51454977482624 29.41615705564053 26.58570252275334 22.483590646779895 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 20645.0 1 15737.0 2 8815.5 3 6349.0 4 5817.5 5 5973.5 6 6321.5 7 6382.5 8 6444.5 9 6692.5 10 7427.0 11 14963.0 12 115728.5 13 136164.5 14 61719.0 15 65444.0 16 74846.0 17 84195.0 18 92736.0 19 100675.5 20 111369.5 21 127416.5 22 149195.0 23 174457.0 24 200502.5 25 228242.0 26 262281.5 27 305236.5 28 355783.0 29 405623.5 30 448456.0 31 489430.0 32 530031.5 33 563071.0 34 591892.5 35 625352.5 36 662948.0 37 700059.0 38 738457.5 39 761703.5 40 767995.0 41 787631.5 42 816639.5 43 820493.0 44 811545.0 45 818718.5 46 825297.5 47 820013.5 48 807656.5 49 793374.5 50 782633.5 51 773391.5 52 759442.0 53 726655.0 54 678577.0 55 631677.5 56 585773.0 57 533478.0 58 475002.0 59 411511.0 60 340976.5 61 276407.0 62 218926.0 63 165176.5 64 122586.0 65 90230.5 66 65528.0 67 48928.5 68 36900.5 69 27740.0 70 21035.0 71 16177.5 72 13223.5 73 10710.5 74 8396.0 75 6794.5 76 5102.0 77 3233.5 78 1934.0 79 1193.5 80 729.0 81 475.5 82 310.5 83 210.5 84 151.0 85 102.0 86 61.5 87 43.0 88 29.5 89 18.0 90 11.0 91 5.5 92 4.5 93 4.5 94 3.0 95 1.5 96 2.0 97 1.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3288801027935027 2 0.006133036363026816 3 2.3935405780593326 4 8.540173692038637E-4 5 4.766608572300634E-5 6 0.008961224115925192 7 0.0077616276252295315 8 2.820243405277875E-4 9 0.0 10-11 7.348521548963478E-5 12-13 5.958260715375792E-6 14-15 0.0 16-17 3.773565119738002E-5 18-19 0.0 20-21 3.4756520839692123E-4 22-23 3.9721738102505285E-5 24-25 4.945356393761907E-4 26-27 0.0037020659911534922 28-29 0.006424991138080229 30-31 0.00579142941534527 32-33 0.015515310902838563 34-35 0.010154862345905475 36-37 0.005314768558115207 38-39 0.005509405074817482 40-41 0.008552090213469388 42-43 0.01912800298326142 44-45 0.034381150414623446 46-47 0.03319942870607392 48-49 0.04009512244066883 50-51 0.04018052417758922 52-53 0.06209897926255163 54-55 0.03307629131795615 56-57 0.022339505508848972 58-59 0.016873794345944243 60-61 0.01810318214021678 62-63 0.02504058369981933 64-65 0.044845842317728465 66-67 0.04744563007653744 68-69 0.05051413434495597 70-71 0.061074158419506995 72-73 0.06809696171602993 74-75 0.05938797063705565 76-77 0.059389956723960774 78-79 0.05382096904198953 80-81 0.05128473606414457 82-83 0.04783291702303686 84-85 0.04991235001270301 86-87 0.05790833589273733 88-89 0.06445845050584044 90-91 0.06038895843723877 92-93 0.06479012701899636 94-95 0.07294102767763044 96-97 0.07203934422270357 98-99 0.047310576166988916 100 0.0066891406964618896 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2.5175132E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 39.09659560883836 #Duplication Level Percentage of deduplicated Percentage of total 1 65.95423970717997 25.785862385200048 2 17.84278758882939 13.951845017897254 3 6.391149750533204 7.4961659181637454 4 2.8453488624687386 4.44973815368034 5 1.6651412956208977 3.2550677933233705 6 1.0503991825681194 2.4640219241232084 7 0.7207783237306922 1.972598505256068 8 0.5225800150721903 1.6344879618030466 9 0.4194632653355186 1.4759627091827072 >10 2.2427643020100296 16.171687278957684 >50 0.21194306567884125 5.8490583245537815 >100 0.13198814040357384 8.367613141264401 >500 6.20261865908002E-4 0.1548933055618505 >1k 7.14701393685727E-4 0.5280041744665083 >5k 4.076865470817684E-5 0.11091261748812888 >10k+ 4.076865470807779E-5 6.332080789077825 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 1497496 5.948314392154925 No Hit AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 38207 0.15176484476824192 No Hit TAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 32820 0.13036674445242233 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.001151930404972653 3.972173810250528E-6 0.0 0.0 1.9860869051252642E-5 2 0.0012075408383161606 3.972173810250528E-6 0.0 0.0 1.9860869051252642E-5 3 0.00127903996690067 3.972173810250528E-6 0.0 0.0 2.78052166717537E-5 4 0.001294928662141672 7.944347620501056E-6 0.0 3.972173810250528E-6 2.78052166717537E-5 5 0.0012989008359519227 1.5888695241002113E-5 0.0 3.972173810250528E-6 3.1777390482004225E-5 6 0.0013108173573826744 1.9860869051252642E-5 0.0 7.944347620501056E-6 3.5749564292254755E-5 7 0.0013187617050031753 2.78052166717537E-5 0.0 7.944347620501056E-6 3.5749564292254755E-5 8 0.0013346504002441774 3.9721738102505285E-5 0.0 7.944347620501056E-6 3.9721738102505285E-5 9 0.0013584834431056806 4.369391191275581E-5 0.0 7.944347620501056E-6 3.9721738102505285E-5 10-11 0.001443885180026067 4.766608572300634E-5 0.0 9.930434525626321E-6 4.369391191275581E-5 12-13 0.0015292869169464534 6.156869405888319E-5 0.0 1.3902608335876848E-5 5.56104333435074E-5 14-15 0.0016444799574437188 7.149912858450951E-5 0.0 1.7874782146127377E-5 6.156869405888319E-5 16-17 0.0017179651729333535 7.547130239476004E-5 0.0 1.9860869051252642E-5 6.355478096400845E-5 18-19 0.0018371303872408693 8.738782382551162E-5 0.0 2.383304286150317E-5 7.547130239476004E-5 20-21 0.0019145877765407546 9.731825835113795E-5 0.0 2.383304286150317E-5 7.547130239476004E-5 22-23 0.0020258086432277693 1.0724869287676426E-4 0.0 2.383304286150317E-5 7.547130239476004E-5 24-25 0.002309819070660682 1.112208666870148E-4 0.0 2.383304286150317E-5 7.944347620501057E-5 26-27 0.003179725135105548 1.1916521430751584E-4 0.0 2.78052166717537E-5 8.341565001526109E-5 28-29 0.005209505952143568 1.4498434407414428E-4 0.0 2.979130357687896E-5 8.937391073063689E-5 30-31 0.010089321478036341 1.6285912622027165E-4 0.0 3.1777390482004225E-5 9.533217144601268E-5 32-33 0.01947159601784809 1.648452131253969E-4 0.0 3.1777390482004225E-5 9.533217144601268E-5 34-35 0.0362480721054412 1.6683130003052217E-4 0.0 3.1777390482004225E-5 9.533217144601268E-5 36-37 0.06290533054603249 1.7278956074589797E-4 0.0 3.5749564292254755E-5 9.731825835113795E-5 38-39 0.10022986175405157 1.7874782146127377E-4 0.0 3.5749564292254755E-5 1.0724869287676426E-4 40-41 0.15108560304668908 1.8073390836639903E-4 0.0 3.9721738102505285E-5 1.112208666870148E-4 42-43 0.2150137683488611 1.946365167022759E-4 0.0 3.9721738102505285E-5 1.112208666870148E-4 44-45 0.2946022288979458 2.005947774176517E-4 0.0 3.9721738102505285E-5 1.1519304049726531E-4 46-47 0.3963693219165644 2.0853912503815273E-4 0.0 4.369391191275581E-5 1.1916521430751584E-4 48-49 0.5236616038398527 2.2045564646890433E-4 0.0 4.369391191275581E-5 1.2512347502289164E-4 50-51 0.6737958712589869 2.4031651552015697E-4 0.0 4.567999881788107E-5 1.3505390954851796E-4 52-53 0.8419022390825994 2.4826086314065805E-4 0.0 4.766608572300634E-5 1.549147785997706E-4 54-55 1.0326877332758375 2.7209390600216115E-4 0.0 4.766608572300634E-5 1.7080347384077271E-4 56-57 1.254797790136711 2.8798260124316327E-4 0.0 4.96521726281316E-5 1.7080347384077271E-4 58-59 1.514075477340099 3.0784347029441596E-4 0.0 5.163825953325687E-5 1.7477564765102323E-4 60-61 1.8039210281002698 3.257182524405433E-4 0.0 5.163825953325687E-5 1.7477564765102323E-4 62-63 2.1192679347222487 3.296904262507938E-4 0.0 5.163825953325687E-5 1.7477564765102323E-4 64-65 2.458185720734255 3.376347738712949E-4 0.0 5.163825953325687E-5 1.7874782146127377E-4 66-67 2.8332026223338174 3.4160694768154543E-4 0.0 5.163825953325687E-5 1.8470608217664958E-4 68-69 3.2469660933654687 3.4756520839692123E-4 0.0 5.163825953325687E-5 2.0853912503815273E-4 70-71 3.697102759977584 3.5153738220717175E-4 0.0 5.163825953325687E-5 2.1449738575352853E-4 72-73 4.17426808328155 3.6742607744817387E-4 0.0 5.362434643838213E-5 2.164834726586538E-4 74-75 4.682475944912623 3.853008595943012E-4 0.0 5.56104333435074E-5 2.3038608099453065E-4 76-77 5.221823663129154 3.932452072148023E-4 0.0 5.56104333435074E-5 2.3435825480478116E-4 78-79 5.797923919525029 4.0118955483530334E-4 0.0 5.958260715375792E-5 2.3435825480478116E-4 80-81 6.40558111075644 4.091339024558044E-4 0.0 6.752695477425898E-5 2.3435825480478116E-4 82-83 7.039450676961694 4.21050423886556E-4 0.0 7.547130239476004E-5 2.3833042861503168E-4 84-85 7.702680565885414 4.2899477150705706E-4 0.0 7.547130239476004E-5 2.48260863140658E-4 86-87 8.396216154894441 4.428973798429339E-4 0.0 8.341565001526109E-5 2.6216347147653483E-4 88 8.93598889570867 4.448834667480592E-4 0.0 8.341565001526109E-5 2.661356452867854E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AAGCAGT 192250 0.0 84.32562 1 GGTATCA 202605 0.0 83.03192 8 AGTGGTA 201505 0.0 82.78068 5 GCAGTGG 201470 0.0 82.75201 3 TGGTATC 203925 0.0 82.42965 7 GTGGTAT 204365 0.0 81.59233 6 GTATCAA 206160 0.0 81.490845 9 AGCAGTG 205690 0.0 80.67902 2 CAGTGGT 210735 0.0 79.00632 4 CAACGCA 200520 0.0 43.29366 12-13 ATCAACG 201895 0.0 43.08018 10-11 ACGCAGA 202480 0.0 42.98259 14-15 GAGTACT 194420 0.0 42.921597 20-21 AGTACTT 201940 0.0 42.697052 20-21 AGAGTAC 203650 0.0 42.62193 18-19 TCAACGC 200575 0.0 41.83668 12-13 AACGCAG 201310 0.0 41.792564 14-15 CGCAGAG 203195 0.0 41.397022 16-17 GTACTTT 203600 0.0 41.00026 22-23 TATCAAC 206360 0.0 40.661575 10-11 >>END_MODULE