FastQCFastQC Report
Thu 26 May 2016
SRR1660721_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660721_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26148704
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT15508645.930940210268164No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT329040.12583415223943795No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA301630.11535179716746193No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1953400.084.9583748
AGTGGTA1968650.084.471765
GTGGTAT1972500.084.292446
TGGTATC1971500.083.945077
GTATCAA1980850.083.6525049
AAGCAGT2007800.083.375611
GCAGTGG2050100.081.744173
CAGTGGT2059900.080.705654
AGCAGTG2090550.080.1669542
CAACGCA1929200.044.30343212-13
ATCAACG1946700.043.99006710-11
ACGCAGA1954700.043.79484614-15
AGAGTAC1941550.043.35854718-19
TCAACGC1929100.043.17508712-13
AACGCAG1940250.043.0597514-15
GAGTACT1893000.043.0506620-21
AGTACTT1967700.042.4709920-21
CGCAGAG1974500.042.23694616-17
TATCAAC1984850.041.7060110-11
GTACTTT1973600.041.2527222-23
Produced by FastQC (version 0.11.5)