##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660721_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 26148704 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.735153719281843 34.0 31.0 34.0 30.0 34.0 2 31.855670246601896 34.0 31.0 34.0 30.0 34.0 3 31.915724427489792 34.0 31.0 34.0 30.0 34.0 4 35.311218483332866 37.0 35.0 37.0 33.0 37.0 5 35.27477476512794 37.0 35.0 37.0 33.0 37.0 6 35.35758839137878 37.0 35.0 37.0 33.0 37.0 7 35.3044805968204 37.0 35.0 37.0 33.0 37.0 8 35.31934794932858 37.0 35.0 37.0 33.0 37.0 9 36.979863437973826 39.0 37.0 39.0 33.0 39.0 10-11 36.90609498275708 39.0 37.0 39.0 33.0 39.0 12-13 36.937325995200375 39.0 37.0 39.0 33.0 39.0 14-15 38.295124702929826 40.0 38.0 41.0 33.0 41.0 16-17 38.27040169944942 40.0 38.0 41.0 33.0 41.0 18-19 38.229807106310126 40.0 38.0 41.0 33.0 41.0 20-21 38.102421768206945 40.0 38.0 41.0 33.0 41.0 22-23 37.91702013224059 40.0 38.0 41.0 32.5 41.0 24-25 37.90536974604936 40.0 38.0 41.0 32.0 41.0 26-27 37.89461391662088 40.0 38.0 41.0 32.0 41.0 28-29 37.813499743620184 40.0 38.0 41.0 32.0 41.0 30-31 37.72074011775115 40.0 38.0 41.0 32.0 41.0 32-33 37.59274021764138 40.0 38.0 41.0 31.0 41.0 34-35 37.431819947940824 40.0 37.0 41.0 31.0 41.0 36-37 37.23351979509195 40.0 37.0 41.0 30.5 41.0 38-39 36.783506708401305 39.0 35.0 41.0 30.0 41.0 40-41 36.71607390178879 39.0 35.0 41.0 30.0 41.0 42-43 36.718661448766255 39.0 35.0 41.0 30.0 41.0 44-45 36.717888542392004 39.5 35.0 41.0 30.0 41.0 46-47 36.81096095622942 40.0 35.0 41.0 30.0 41.0 48-49 36.69981020856713 40.0 35.0 41.0 30.0 41.0 50-51 35.33154363979186 38.0 34.0 40.0 27.5 40.5 52-53 35.48911328454366 38.5 34.5 39.5 28.0 40.5 54-55 35.83985336328715 39.0 35.0 41.0 26.0 41.0 56-57 35.43456958708164 39.0 35.0 41.0 23.0 41.0 58-59 35.243512584791965 39.0 35.0 41.0 23.0 41.0 60-61 35.02872819241826 39.0 35.0 41.0 23.0 41.0 62-63 34.81109036990897 38.0 34.0 41.0 22.0 41.0 64-65 34.57749479668285 37.5 34.0 40.0 22.0 41.0 66-67 34.33646028881584 37.0 34.0 40.0 22.0 41.0 68-69 34.08792672860575 36.5 34.0 40.0 22.0 41.0 70-71 33.819147843809006 36.0 33.5 39.0 22.0 41.0 72-73 33.50602345339945 35.5 33.0 39.0 22.0 41.0 74-75 33.151243499486625 35.0 33.0 38.5 21.0 40.5 76-77 32.777460385799614 35.0 33.0 37.0 20.0 39.5 78-79 32.4218364703658 35.0 32.5 37.0 20.0 39.0 80-81 32.11920143346301 35.0 32.0 36.5 19.5 39.0 82-83 31.82432049022391 35.0 32.0 36.0 19.0 38.0 84-85 31.55147065797219 35.0 32.0 36.0 18.5 37.0 86-87 31.346947118296953 35.0 32.0 35.0 18.0 37.0 88-89 31.148387201140064 35.0 31.5 35.0 18.0 36.0 90-91 30.982511523324447 35.0 31.0 35.0 18.0 36.0 92-93 30.83515636186023 35.0 31.0 35.0 17.0 36.0 94-95 30.662545665743128 35.0 31.0 35.0 17.0 36.0 96-97 30.533237211297354 35.0 31.0 35.0 15.5 35.0 98-99 30.438366448295106 35.0 31.0 35.0 15.0 35.0 100 30.36586838108688 34.0 31.0 35.0 15.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5923024272838902 1101 2 0.5847110697374198 1101 3 0.5930225265140372 1101 4 0.5251946060737467 1101 5 0.550106075325445 1101 6 0.5049878659705342 1101 7 0.5111847199793047 1101 8 0.5206589633796881 1101 9 0.6152768371016251 1101 10-11 0.6428244822663132 1101 12-13 0.61349680077398 1101 14-15 0.7182261055081867 1101 16-17 0.7095304570185377 1101 18-19 0.7162075165258557 1101 20-21 0.7546271890980591 1101 22-23 0.7747197396408083 1101 24-25 0.7917731567963884 1101 26-27 0.7236916035571639 1101 28-29 0.7209471708458111 1101 30-31 0.6899122078290532 1101 32-33 0.664943553639695 1101 34-35 0.7230060265028087 1101 36-37 0.6753080183517568 1101 38-39 0.6867193141283963 1101 40-41 0.6719997782209504 1101 42-43 0.6781208258577038 1101 44-45 0.6870017595605731 1101 46-47 0.7131764156227476 1101 48-49 0.7578972353992199 1101 50-51 0.6983534599017744 1101 52-53 0.7046544100256895 1101 54-55 0.7519947680577204 1101 56-57 0.7565942089272539 1101 58-59 0.6733604979929098 1101 60-61 0.6638602031411054 1101 62-63 0.6249990468578162 1101 64-65 0.5568150028074825 1101 66-67 0.566297734294416 1101 68-69 0.5534531129167846 1101 70-71 0.5359386361263461 1101 72-73 0.5345442233591129 1101 74-75 0.49999541384811863 1101 76-77 0.4398863810318545 1101 78-79 0.48548779257545505 1101 80-81 0.4068218470616287 1101 82-83 0.4191101782849884 1101 84-85 0.39746814128741903 1101 86-87 0.3721394204823518 1101 88-89 0.3889983801922199 1101 90-91 0.3797430439125229 1101 92-93 0.36576566967040947 1101 94-95 0.3893643854679034 1101 96-97 0.33368060639215 1101 98-99 0.31610069383027906 1101 100 0.3143310518333493 1102 1 0.44699035620011074 1102 2 0.47716822919228363 1102 3 0.48539741408391635 1102 4 0.4143075530204783 1102 5 0.4377957769888994 1102 6 0.3710970129335962 1102 7 0.4194443752260213 1102 8 0.4234925594458403 1102 9 0.48068484669448 1102 10-11 0.4948093165371219 1102 12-13 0.500059834733058 1102 14-15 0.5597606915849056 1102 16-17 0.5557237639130364 1102 18-19 0.5016404862579691 1102 20-21 0.52215832896389 1102 22-23 0.564757986504425 1102 24-25 0.5511627323906794 1102 26-27 0.5601270912910792 1102 28-29 0.5870538132416243 1102 30-31 0.5494875889829629 1102 32-33 0.5171387114878669 1102 34-35 0.5153631703037789 1102 36-37 0.5112261409734202 1102 38-39 0.5392262348871455 1102 40-41 0.4933117530130815 1102 42-43 0.4930939250518591 1102 44-45 0.5002709459500352 1102 46-47 0.540375061310904 1102 48-49 0.5432879569090758 1102 50-51 0.5036125664709559 1102 52-53 0.5193757638789762 1102 54-55 0.5324367854009253 1102 56-57 0.5819415850884511 1102 58-59 0.5051449946720865 1102 60-61 0.5310872106455875 1102 62-63 0.5375714680666874 1102 64-65 0.517249802811456 1102 66-67 0.49141977555830607 1102 68-69 0.49806142947870313 1102 70-71 0.48442599542627107 1102 72-73 0.43220554024366464 1102 74-75 0.43300794644874685 1102 76-77 0.3775003481668193 1102 78-79 0.4132416631312452 1102 80-81 0.3397017683979442 1102 82-83 0.3444343918983037 1102 84-85 0.3031228053515349 1102 86-87 0.27658022883558786 1102 88-89 0.2997392002358268 1102 90-91 0.29384503679289864 1102 92-93 0.2526770467861752 1102 94-95 0.2878476548143709 1102 96-97 0.2569054365179184 1102 98-99 0.1963405520008905 1102 100 0.22279846311266738 1103 1 0.3749155717982333 1103 2 0.39445437255384874 1103 3 0.4351304413265247 1103 4 0.37375738269294345 1103 5 0.3833605712843138 1103 6 0.35428048366186715 1103 7 0.3219663679659064 1103 8 0.3540619466248458 1103 9 0.4161339976972869 1103 10-11 0.39830614374535145 1103 12-13 0.44944976128521574 1103 14-15 0.5264490945961953 1103 16-17 0.49411698441483765 1103 18-19 0.4729341447060733 1103 20-21 0.4932793018512811 1103 22-23 0.5009173976338062 1103 24-25 0.5492583137985037 1103 26-27 0.5143554882034493 1103 28-29 0.5020487333433081 1103 30-31 0.43748240096810775 1103 32-33 0.4003931937657157 1103 34-35 0.42563505727078166 1103 36-37 0.41132698194742545 1103 38-39 0.44650677808878214 1103 40-41 0.40196070752973156 1103 42-43 0.45004856926146886 1103 44-45 0.4797625239727594 1103 46-47 0.48362788464595496 1103 48-49 0.5480330976378056 1103 50-51 0.4295231402379258 1103 52-53 0.47504808212925553 1103 54-55 0.5403162824872894 1103 56-57 0.5657893915645857 1103 58-59 0.4901861609606115 1103 60-61 0.5074967013432925 1103 62-63 0.4765916395234129 1103 64-65 0.4589560351188666 1103 66-67 0.4009669102086306 1103 68-69 0.447200848173388 1103 70-71 0.40895523388644506 1103 72-73 0.39282551875596283 1103 74-75 0.4243221407268294 1103 76-77 0.3907398143271479 1103 78-79 0.3735167235167509 1103 80-81 0.30345824907985985 1103 82-83 0.28662565412172114 1103 84-85 0.3109873081332566 1103 86-87 0.2552156870044584 1103 88-89 0.2994996970554631 1103 90-91 0.2618539937814077 1103 92-93 0.21277714117406532 1103 94-95 0.2394181247542697 1103 96-97 0.20612692729043403 1103 98-99 0.22424828764543392 1103 100 0.19918991225259575 1104 1 0.3634806668611077 1104 2 0.41798817435699576 1104 3 0.3937980023523515 1104 4 0.35513237781336215 1104 5 0.36093457311838506 1104 6 0.311182889469265 1104 7 0.32749059916872625 1104 8 0.31592760706148937 1104 9 0.3948363376941231 1104 10-11 0.3442027130384204 1104 12-13 0.353365044808875 1104 14-15 0.473016287597666 1104 16-17 0.4253605289258502 1104 18-19 0.4285181450359232 1104 20-21 0.3574898969741511 1104 22-23 0.41048781546231083 1104 24-25 0.41803083293119414 1104 26-27 0.36890974196905546 1104 28-29 0.34606079766410147 1104 30-31 0.3366559047366877 1104 32-33 0.3387611407611075 1104 34-35 0.31754779494510643 1104 36-37 0.3222367056407549 1104 38-39 0.3201933190276449 1104 40-41 0.34770668316200215 1104 42-43 0.3170577359230222 1104 44-45 0.31883789334145973 1104 46-47 0.319991152655291 1104 48-49 0.364394575923491 1104 50-51 0.3301782760850429 1104 52-53 0.3615722676122104 1104 54-55 0.4190578913583707 1104 56-57 0.4624504893643433 1104 58-59 0.39308608134155776 1104 60-61 0.3956903062240613 1104 62-63 0.3527749511969276 1104 64-65 0.35162274528909165 1104 66-67 0.3290549383591852 1104 68-69 0.3292984236530643 1104 70-71 0.316234931397787 1104 72-73 0.31652839580699066 1104 74-75 0.2674448011609698 1104 76-77 0.26304999883147673 1104 78-79 0.2716608834260583 1104 80-81 0.27436460067611534 1104 82-83 0.283243013022215 1104 84-85 0.26704958657483857 1104 86-87 0.25068732716760067 1104 88-89 0.20692654724226145 1104 90-91 0.18621466453226887 1104 92-93 0.20222546417181775 1104 94-95 0.24585224553059604 1104 96-97 0.23999916610962302 1104 98-99 0.20812469060524208 1104 100 0.15716416785591036 1105 1 0.29595698716946117 1105 2 0.3547793634550125 1105 3 0.34871560703863835 1105 4 0.30832184829485954 1105 5 0.33542198183152294 1105 6 0.31718259628048884 1105 7 0.31383304726671923 1105 8 0.3112176734704164 1105 9 0.3481897519276558 1105 10-11 0.3734773198527037 1105 12-13 0.3543061576237463 1105 14-15 0.4136981226865686 1105 16-17 0.38087728187170455 1105 18-19 0.39689488759789526 1105 20-21 0.4240809519106321 1105 22-23 0.4732490630101651 1105 24-25 0.49540039803106595 1105 26-27 0.4806430301442788 1105 28-29 0.4719487716215198 1105 30-31 0.4844300864401134 1105 32-33 0.46724356191868566 1105 34-35 0.4559656605153535 1105 36-37 0.4307537495874101 1105 38-39 0.4673231064155843 1105 40-41 0.46881896841537696 1105 42-43 0.5143144001760547 1105 44-45 0.5069619201747955 1105 46-47 0.48132903817987227 1105 48-49 0.5283206600462762 1105 50-51 0.4166275360214797 1105 52-53 0.4593793204369021 1105 54-55 0.5717929160142816 1105 56-57 0.5480966623755847 1105 58-59 0.5167887209929845 1105 60-61 0.5187865354662833 1105 62-63 0.523687956886441 1105 64-65 0.5187539213982504 1105 66-67 0.4804296436581268 1105 68-69 0.4993406342140858 1105 70-71 0.49796471430680356 1105 72-73 0.4649893316841087 1105 74-75 0.4156891435055883 1105 76-77 0.45714123781533544 1105 78-79 0.4907038292757093 1105 80-81 0.4181346480058963 1105 82-83 0.3810893367978707 1105 84-85 0.3855755233567493 1105 86-87 0.3379785138202216 1105 88-89 0.36582209494284257 1105 90-91 0.38202218725697534 1105 92-93 0.36085939751680485 1105 94-95 0.42810029237593383 1105 96-97 0.42547803296459463 1105 98-99 0.39585596931760136 1105 100 0.38755822361428827 1106 1 0.22157414518088814 1106 2 0.2658585516059411 1106 3 0.25511201593921484 1106 4 0.26380743185094957 1106 5 0.2613931783868395 1106 6 0.23923756937152518 1106 7 0.2607378445298991 1106 8 0.27392469797813135 1106 9 0.2530539161253742 1106 10-11 0.2529562160079948 1106 12-13 0.26726307286943296 1106 14-15 0.31726345510290344 1106 16-17 0.32072793635254015 1106 18-19 0.26608693786020865 1106 20-21 0.2933956550528478 1106 22-23 0.3046695939935802 1106 24-25 0.31303470820422774 1106 26-27 0.30659664081122173 1106 28-29 0.28295999813243355 1106 30-31 0.2735942631742816 1106 32-33 0.2861822457534089 1106 34-35 0.30517984258943187 1106 36-37 0.2395813703717451 1106 38-39 0.24742642008033755 1106 40-41 0.2847996676222948 1106 42-43 0.28329344483669416 1106 44-45 0.31070910291042964 1106 46-47 0.31725301596943467 1106 48-49 0.3183058745411316 1106 50-51 0.2014002243998405 1106 52-53 0.29345583399494757 1106 54-55 0.34054212625073177 1106 56-57 0.3532691683305771 1106 58-59 0.307183330479738 1106 60-61 0.30738768363679014 1106 62-63 0.33127282320765516 1106 64-65 0.302513069241769 1106 66-67 0.2851255798551833 1106 68-69 0.26722122316982677 1106 70-71 0.32295316030788257 1106 72-73 0.27720332260895475 1106 74-75 0.25707786531345533 1106 76-77 0.26843272773091087 1106 78-79 0.28027721850299514 1106 80-81 0.23191877798825544 1106 82-83 0.22108973245526897 1106 84-85 0.24278264028185603 1106 86-87 0.19477276523460674 1106 88-89 0.20996532738491425 1106 90-91 0.18698387212091916 1106 92-93 0.19169556126455944 1106 94-95 0.21019403678918636 1106 96-97 0.2465574398707524 1106 98-99 0.23376437820766682 1106 100 0.22900921401215157 1107 1 0.172063564115156 1107 2 0.18500921978467844 1107 3 0.1855214252036994 1107 4 0.21227526354692117 1107 5 0.22004747154868198 1107 6 0.1912801198539782 1107 7 0.1842985106346262 1107 8 0.1935320390427151 1107 9 0.2269827295788147 1107 10-11 0.22038816661550698 1107 12-13 0.230199199266373 1107 14-15 0.23936665596868778 1107 16-17 0.21179237069726042 1107 18-19 0.23103847487520568 1107 20-21 0.23449316996812541 1107 22-23 0.26410302419223797 1107 24-25 0.2814374201348002 1107 26-27 0.25252660681525896 1107 28-29 0.25469890410050766 1107 30-31 0.19974577315921493 1107 32-33 0.12735534002699467 1107 34-35 0.17123890028619826 1107 36-37 0.17827043695243816 1107 38-39 0.1846028883321864 1107 40-41 0.2155568874678977 1107 42-43 0.2942219886343267 1107 44-45 0.28860239930831 1107 46-47 0.29458459680614624 1107 48-49 0.342096342450553 1107 50-51 0.25568516092260296 1107 52-53 0.2862338607278403 1107 54-55 0.4211273203071002 1107 56-57 0.4405979130960489 1107 58-59 0.3985448220618508 1107 60-61 0.43911590745582174 1107 62-63 0.39078644254851014 1107 64-65 0.3851841106057208 1107 66-67 0.37513840758966666 1107 68-69 0.33503124456625955 1107 70-71 0.34730558456561766 1107 72-73 0.35315818918559927 1107 74-75 0.3773493930651455 1107 76-77 0.34375312018081416 1107 78-79 0.36324889909976577 1107 80-81 0.2929771923390305 1107 82-83 0.2985089224047144 1107 84-85 0.33270113391931133 1107 86-87 0.3125542235426302 1107 88-89 0.29418046432946454 1107 90-91 0.3164198123535691 1107 92-93 0.31172441068901335 1107 94-95 0.31481522639675674 1107 96-97 0.3318645422175237 1107 98-99 0.32209796356010045 1107 100 0.3085365398459139 1108 1 0.07822492571690276 1108 2 0.13251285865417017 1108 3 0.1550545897505664 1108 4 0.11859959098923412 1108 5 0.14749469264368997 1108 6 0.08218186902102076 1108 7 0.10314221446355276 1108 8 0.1276069750728368 1108 9 0.13196511889636753 1108 10-11 0.1159865573718335 1108 12-13 0.1115984831029877 1108 14-15 0.13634182485359503 1108 16-17 0.12721348263357157 1108 18-19 0.09291192680089466 1108 20-21 0.1098149951338101 1108 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-1.504127204757296 2316 98-99 -1.3719469107605349 2316 100 -1.1297822075651638 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 13523.0 3 3375.0 4 2371.0 5 2055.0 6 3631.0 7 18427.0 8 34354.0 9 39899.0 10 41798.0 11 41882.0 12 43313.0 13 48369.0 14 55210.0 15 64121.0 16 75064.0 17 86544.0 18 96502.0 19 109218.0 20 127392.0 21 153695.0 22 195429.0 23 281864.0 24 424342.0 25 519883.0 26 498463.0 27 420841.0 28 376558.0 29 367235.0 30 406358.0 31 486507.0 32 606035.0 33 785019.0 34 1088224.0 35 1686268.0 36 2891227.0 37 5188768.0 38 6708203.0 39 2150085.0 40 6652.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 16.394414831962774 33.72322507093319 26.90465340686088 22.97770669024316 2 21.020187687445578 33.85124748728864 26.777160701424624 18.35140412384116 3 26.051456408457323 25.32739420389378 26.73376701011979 21.88738237752911 4 19.246401545523927 27.43724852729986 24.85097921160289 28.465370715573318 5 21.536471395011 32.82174939349874 25.922447374719393 19.719331836770866 6 28.39995958924512 26.789876592920102 25.53890034740769 19.271263470427087 7 20.871963974447933 25.78589143201386 32.787224379891256 20.554920213646955 8 27.35183843354004 26.1151659660776 25.81036376504326 20.7226318353391 9 26.844453111034284 24.870053488960234 27.253512917601512 21.031980482403974 10-11 20.579410954342958 29.604684242340344 29.221580355469218 20.59432444784748 12-13 20.497953811192758 26.237342430671482 29.152664610151113 24.112039147984643 14-15 20.490020438980434 33.1148956394161 25.79577219096143 20.599311730642043 16-17 24.05862232249286 26.013432517314378 25.853120647851675 24.074824512341088 18-19 20.563203646186647 29.493364882628846 25.843323053038596 24.100108418145908 20-21 24.0427487355545 29.575350734464458 25.801732663333212 20.580167866647837 22-23 24.025338422756455 26.117606129556727 29.247877738083027 20.60917770960379 24-25 20.633036873068804 29.383320785093332 25.75391884722043 24.22972349461744 26-27 20.687420459332028 25.950541185149994 32.73136153910945 20.630676816408524 28-29 20.703067406997395 26.026108695461186 32.69706675534517 20.573757142196254 30-31 20.638590126004082 25.924166707134372 32.71881697688089 20.71842618998066 32-33 20.733139936030124 26.055407458349123 32.55313075809959 20.65832184752117 34-35 20.778382728573845 26.156712140351612 32.44766530757393 20.617239823500615 36-37 20.71165144304981 26.105539763703394 32.49660878782231 20.686200005424485 38-39 20.74980401926832 26.076835548374337 32.52460162068926 20.648758811668085 40-41 20.755545632968957 26.099781102073976 32.48620972925694 20.658463535700122 42-43 20.723892137132648 26.122285758456947 32.464999902418754 20.688822201991652 44-45 20.75032054190827 26.1753998969467 32.45078663526275 20.623492925882275 46-47 20.73690797535962 26.26628033432718 32.405335250577934 20.591476439735267 48-49 20.795639599815637 26.242960920442336 32.307218496577754 20.654180983164274 50-51 20.785015221521117 26.27422579177902 32.26614888816632 20.674610098533538 52-53 20.871325735800337 26.404362489750067 32.02416300239663 20.700148772052973 54-55 21.210223216625458 26.655984016452138 31.211044782256202 20.922747984666202 56-57 21.68777553098594 27.22066494310394 29.998714429601257 21.092845096308864 58-59 21.77694574887626 27.764412675488153 29.306464031897843 21.152177543737746 60-61 21.85075426347112 28.012341753610194 28.928507858659003 21.208396124259682 62-63 21.817828139742193 28.159929568916848 28.816624152136466 21.20561813920449 64-65 21.806397430636657 28.2407367259244 28.807409068637373 21.14545677480157 66-67 21.791015578221334 28.055823572762183 29.05074352111679 21.10241732789969 68-69 21.799543409926546 27.882490491727495 29.284083552177187 21.033882546168776 70-71 21.79652118479998 27.732797382441454 29.4510852017635 21.01959623099507 72-73 21.829962556022487 27.63403367687577 29.50761278687386 21.028390980227883 74-75 21.95579350470121 27.71798516166576 29.382744139856854 20.943477193776182 76-77 22.144709279852293 27.88570169054826 29.082851765681696 20.886737263917755 78-79 22.328568843718884 27.98928351639533 28.784648550901366 20.89749908898442 80-81 22.520726457127683 28.146556630909085 28.450626277233688 20.882090634729547 82-83 22.595630870347744 28.31223878432488 28.20137903078564 20.89075131454174 84-85 22.64103799504655 28.356628320194165 28.06570835709696 20.936625327662327 86-87 22.73490887147884 28.456920983528132 27.942761134674964 20.86540901031806 88-89 22.886818409580563 28.502379901489082 27.787931718618353 20.822869970312002 90-91 23.0158531677612 28.489799852904884 27.633015224696905 20.86133175463701 92-93 23.11351552776715 28.53817656651842 27.549723619637277 20.79858428607716 94-95 23.20606610902666 28.586432713719574 27.459493452125454 20.748007725128314 96-97 23.306472619830455 28.575574847190698 27.367509989361338 20.750442543617506 98-99 23.42706848229079 28.569373997104414 27.31789125810379 20.68566626250101 100 23.49945348906084 28.592287600563317 27.220042989564735 20.688215920811107 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 38898.0 1 26191.0 2 11363.0 3 8673.0 4 7932.5 5 7720.5 6 7773.5 7 7824.0 8 7714.0 9 7793.0 10 8231.0 11 10845.0 12 113179.0 13 140392.0 14 65753.0 15 68298.0 16 77450.5 17 86870.0 18 96112.0 19 105692.0 20 117197.5 21 133517.0 22 155901.5 23 181623.0 24 207902.5 25 238406.0 26 272676.0 27 311007.0 28 359264.5 29 407378.0 30 446512.5 31 486562.5 32 530095.5 33 566535.5 34 598219.0 35 635842.5 36 679770.5 37 718045.0 38 751776.0 39 775382.0 40 784142.0 41 800124.0 42 820635.5 43 819372.5 44 808503.5 45 810530.0 46 814185.5 47 812221.0 48 805245.0 49 791876.0 50 777874.5 51 767978.0 52 756361.0 53 727400.0 54 687248.0 55 647659.0 56 604025.5 57 556414.5 58 504930.5 59 444431.0 60 377674.0 61 319838.0 62 269889.0 63 222177.0 64 182341.5 65 150072.5 66 122700.5 67 101531.0 68 83684.5 69 67737.5 70 54502.5 71 44820.5 72 36976.5 73 28344.5 74 20841.0 75 15797.5 76 11970.0 77 8523.0 78 5933.5 79 4399.0 80 3364.0 81 2626.5 82 2084.5 83 1676.0 84 1393.5 85 1154.5 86 958.0 87 858.0 88 770.5 89 667.0 90 576.0 91 491.0 92 443.0 93 380.0 94 307.5 95 284.0 96 265.0 97 215.0 98 168.0 99 129.0 100 85.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4089877647473466 2 0.3868031088653572 3 0.16948067483573945 4 0.17580221184193295 5 0.27726421928979733 6 0.10304525991039555 7 0.13731082045213408 8 0.14281396125788873 9 0.1363432772805872 10-11 0.13296643688344936 12-13 0.13491299607047447 14-15 0.14086357778955316 16-17 0.14546036392472836 18-19 0.15675537877517753 20-21 0.1379494754309812 22-23 0.10005275978495914 24-25 0.09505059983087498 26-27 0.08914590948752182 28-29 0.09895136676754612 30-31 0.10344872158864929 32-33 0.10458262099720124 34-35 0.10508933827083744 36-37 0.10098970870602231 38-39 0.100341493023899 40-41 0.10127844194496216 42-43 0.06361309531822304 44-45 0.06717541335891829 46-47 0.0853617831308198 48-49 0.08947097339891108 50-51 0.09875059199874686 52-53 0.10401471522259766 54-55 0.10648711308981126 56-57 0.10709134953686424 58-59 0.10637812107246308 60-61 0.10438184622840198 62-63 0.10868033842136116 64-65 0.11538430355860084 66-67 0.12441343173260136 68-69 0.1293104239506478 70-71 0.12763921301797596 72-73 0.11454104952964399 74-75 0.11242430982430333 76-77 0.11411655430418272 78-79 0.1128239472212466 80-81 0.10724623292993794 82-83 0.1100054518954362 84-85 0.1160745863351392 86-87 0.12989362685049324 88-89 0.13753454090879608 90-91 0.14420599965489683 92-93 0.14339716415773415 94-95 0.13370834745767898 96-97 0.13020339363664066 98-99 0.1252930164340076 100 0.11932904973034227 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2.6148704E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.76818911835659 #Duplication Level Percentage of deduplicated Percentage of total 1 68.76299340091543 32.159206797183195 2 17.46373909031963 16.334949049794105 3 6.247426607318021 8.765424872223063 4 2.566723791548967 4.8016409479098945 5 1.312425905782164 3.0689891482725344 6 0.8350370658693254 2.343190285044852 7 0.5264494222300661 1.7234760298073704 8 0.37218664995263395 1.3925196505849895 9 0.28776036674762073 1.2112228127538338 >10 1.4108637840597833 11.791910290036379 >50 0.14265711933994774 4.719060195646266 >100 0.07079014504190786 4.835421859426497 >500 3.755445219063505E-4 0.1188315886823571 >1k 5.22242816980996E-4 0.507792913261031 >5k 2.443176784117775E-5 0.07927944616908048 >10k+ 2.4431767836247414E-5 6.1470841132044445 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 1550864 5.930940210268164 No Hit AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 32904 0.12583415223943795 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 30163 0.11535179716746193 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 5.889393218111307E-4 0.0 7.648562620923774E-6 0.0 3.824281310461887E-6 2 6.004121657425163E-4 3.824281310461887E-6 7.648562620923774E-6 3.824281310461887E-6 3.824281310461887E-6 3 6.004121657425163E-4 3.824281310461887E-6 7.648562620923774E-6 7.648562620923774E-6 7.648562620923774E-6 4 6.042364470529783E-4 1.529712524184755E-5 7.648562620923774E-6 1.1472843931385662E-5 7.648562620923774E-6 5 6.080607283634401E-4 2.676996917323321E-5 7.648562620923774E-6 1.1472843931385662E-5 7.648562620923774E-6 6 6.080607283634401E-4 2.676996917323321E-5 7.648562620923774E-6 1.1472843931385662E-5 7.648562620923774E-6 7 6.080607283634401E-4 3.05942504836951E-5 7.648562620923774E-6 1.1472843931385662E-5 7.648562620923774E-6 8 6.195335722948258E-4 3.05942504836951E-5 7.648562620923774E-6 1.1472843931385662E-5 1.529712524184755E-5 9 6.195335722948258E-4 3.441853179415699E-5 7.648562620923774E-6 1.1472843931385662E-5 1.529712524184755E-5 10-11 6.233578536052876E-4 4.2067094415080763E-5 7.648562620923774E-6 1.1472843931385662E-5 1.912140655230944E-5 12-13 6.44391400812828E-4 6.11885009673902E-5 7.648562620923774E-6 1.5297125241847552E-5 1.912140655230944E-5 14-15 6.482156821232899E-4 7.457348555400681E-5 7.648562620923774E-6 1.912140655230944E-5 2.676996917323321E-5 16-17 6.577763853994447E-4 7.648562620923775E-5 7.648562620923774E-6 1.912140655230944E-5 2.676996917323321E-5 18-19 6.76897791951754E-4 9.943131407200907E-5 1.1472843931385662E-5 2.676996917323321E-5 4.589137572554265E-5 20-21 6.960191985040634E-4 1.0134345472724002E-4 1.1472843931385662E-5 3.250639113892605E-5 5.5452079001697366E-5 22-23 7.648562620923775E-4 1.0325559538247096E-4 1.1472843931385662E-5 3.441853179415699E-5 7.266134489877587E-5 24-25 9.962252813753218E-4 1.1090415800339473E-4 1.1472843931385662E-5 3.441853179415699E-5 8.795847014062341E-5 26-27 0.0017744665280543157 1.1472843931385663E-4 1.1472843931385662E-5 3.6330672449387934E-5 8.795847014062341E-5 28-29 0.0038013356225991167 1.2811342390047322E-4 1.1472843931385662E-5 4.2067094415080763E-5 8.795847014062341E-5 30-31 0.00808644283097166 1.4149840848708985E-4 1.1472843931385662E-5 4.397923507031171E-5 8.795847014062341E-5 32-33 0.015165187536636614 1.4914697110801362E-4 1.3384984586616606E-5 4.780351638077359E-5 8.795847014062341E-5 34-35 0.027075911678070164 1.529712524184755E-4 1.912140655230944E-5 5.162779769123548E-5 8.987061079585436E-5 36-37 0.04612083260417037 1.6826837766032305E-4 1.912140655230944E-5 5.353993834646642E-5 9.17827514510853E-5 38-39 0.07586226835563246 1.8547764355740154E-4 2.2945687862771325E-5 5.353993834646642E-5 9.560703276154719E-5 40-41 0.11443588179360628 1.9121406552309437E-4 2.2945687862771325E-5 5.7364219656928316E-5 1.0899201734816379E-4 42-43 0.15824111206429198 2.0651119076494192E-4 2.2945687862771325E-5 6.11885009673902E-5 1.1090415800339473E-4 44-45 0.21042534268619967 2.1607189404109663E-4 2.2945687862771325E-5 6.501278227785208E-5 1.1281629865862567E-4 46-47 0.276946803941029 2.3901758190386798E-4 2.4857828518002268E-5 7.074920424354492E-5 1.223770019347804E-4 48-49 0.363792408220308 2.4857828518002267E-4 2.676996917323321E-5 7.83977668644687E-5 1.3002556455570416E-4 50-51 0.46749926879741344 2.638754104218702E-4 3.05942504836951E-5 8.030990751969964E-5 1.3767412717662796E-4 52-53 0.5757723212592104 2.638754104218702E-4 3.05942504836951E-5 8.795847014062341E-5 1.434105491423208E-4 54-55 0.692363185571262 2.73436113698025E-4 3.05942504836951E-5 8.795847014062341E-5 1.567955337289374E-4 56-57 0.8257770633680355 2.791725356637178E-4 3.05942504836951E-5 9.17827514510853E-5 1.6444409634986117E-4 58-59 0.9863528991723645 2.868210982846416E-4 3.250639113892605E-5 9.751917341677813E-5 1.8930192486786343E-4 60-61 1.1686449163981512 3.135910674578748E-4 3.441853179415699E-5 1.0325559538247096E-4 1.969504874887872E-4 62-63 1.3585243077438944 3.2888819269972233E-4 3.441853179415699E-5 1.0325559538247096E-4 2.007747687992491E-4 64-65 1.5548361402538342 3.327124740101842E-4 4.589137572554265E-5 1.0325559538247096E-4 2.0268690945448003E-4 66-67 1.770965398514588 3.327124740101842E-4 4.589137572554265E-5 1.0325559538247096E-4 2.1607189404109663E-4 68-69 2.015124726640372 3.346246146654152E-4 4.589137572554265E-5 1.1090415800339473E-4 2.2563259731725138E-4 70-71 2.2867787252477214 3.460974585968008E-4 4.589137572554265E-5 1.1855272062431852E-4 2.2563259731725138E-4 72-73 2.5649971027244796 3.594824431834174E-4 4.780351638077359E-5 1.1855272062431852E-4 2.2563259731725138E-4 74-75 2.8523708096584826 3.709552871148031E-4 4.9715657036004536E-5 1.1855272062431852E-4 2.2754473797248232E-4 76-77 3.1588410653162775 3.8434027170141966E-4 4.9715657036004536E-5 1.2046486127954945E-4 2.3328115993817515E-4 78-79 3.494247745509682 3.8625241235665063E-4 4.9715657036004536E-5 1.223770019347804E-4 2.3328115993817515E-4 80-81 3.86354903095771 3.881645530118816E-4 4.9715657036004536E-5 1.223770019347804E-4 2.3710544124863704E-4 82-83 4.244130034130945 4.015495375984982E-4 4.9715657036004536E-5 1.223770019347804E-4 2.5240256649048455E-4 84-85 4.63338833159762 4.1493452218511475E-4 4.9715657036004536E-5 1.2620128324524228E-4 2.6961183238756307E-4 86-87 5.0416934621310485 4.244952254612695E-4 4.9715657036004536E-5 1.3002556455570416E-4 2.829968169741797E-4 88 5.363336553888101 4.436166320135789E-4 4.9715657036004536E-5 1.3384984586616608E-4 2.829968169741797E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 195340 0.0 84.958374 8 AGTGGTA 196865 0.0 84.47176 5 GTGGTAT 197250 0.0 84.29244 6 TGGTATC 197150 0.0 83.94507 7 GTATCAA 198085 0.0 83.652504 9 AAGCAGT 200780 0.0 83.37561 1 GCAGTGG 205010 0.0 81.74417 3 CAGTGGT 205990 0.0 80.70565 4 AGCAGTG 209055 0.0 80.166954 2 CAACGCA 192920 0.0 44.303432 12-13 ATCAACG 194670 0.0 43.990067 10-11 ACGCAGA 195470 0.0 43.794846 14-15 AGAGTAC 194155 0.0 43.358547 18-19 TCAACGC 192910 0.0 43.175087 12-13 AACGCAG 194025 0.0 43.05975 14-15 GAGTACT 189300 0.0 43.05066 20-21 AGTACTT 196770 0.0 42.47099 20-21 CGCAGAG 197450 0.0 42.236946 16-17 TATCAAC 198485 0.0 41.70601 10-11 GTACTTT 197360 0.0 41.25272 22-23 >>END_MODULE