FastQCFastQC Report
Thu 26 May 2016
SRR1660721_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660721_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26148704
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT11595884.434590716235879No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT367020.1403587726565722No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1601200.081.836968
AAGCAGT1563800.081.169921
AGTGGTA1607650.080.94585
GTGGTAT1612850.080.626626
TGGTATC1628800.080.442287
GTATCAA1630150.080.3344969
GCAGTGG1642650.079.304953
AGCAGTG1684050.077.054662
CAGTGGT1691800.076.7434
CAACGCA1572900.043.2631412-13
ATCAACG1583900.043.0338110-11
ACGCAGA1596250.042.71564514-15
AGAGTAC1608050.042.32374618-19
GAGTACT1556000.041.77667620-21
TCAACGC1568900.041.6216912-13
AACGCAG1580650.041.4237314-15
AGTACTT1641150.041.22946520-21
CGCAGAG1612450.040.5908616-17
TATCAAC1634850.039.9828710-11
GTACTTT1647500.039.44112822-23
Produced by FastQC (version 0.11.5)