##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660721_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 26148704 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.248275555071487 34.0 31.0 34.0 27.0 34.0 2 31.896615908765497 34.0 31.0 34.0 28.0 34.0 3 31.662805315322704 34.0 31.0 34.0 30.0 34.0 4 35.58116734963232 37.0 35.0 37.0 33.0 37.0 5 35.914135553333736 37.0 35.0 37.0 35.0 37.0 6 35.36906968697187 37.0 35.0 37.0 33.0 37.0 7 35.598992171849126 37.0 35.0 37.0 35.0 37.0 8 35.95649214584402 37.0 35.0 37.0 35.0 37.0 9 37.767576282174446 39.0 38.0 39.0 35.0 39.0 10-11 37.80001280369383 39.0 37.5 39.0 35.0 39.0 12-13 37.85602768305458 39.0 38.0 39.0 35.0 39.0 14-15 39.30605947048083 41.0 39.0 41.0 36.0 41.0 16-17 39.27441769198198 41.0 39.0 41.0 36.0 41.0 18-19 39.27256608969989 41.0 39.0 41.0 36.0 41.0 20-21 39.21713013004392 40.5 39.0 41.0 36.0 41.0 22-23 39.114206654371856 40.0 39.0 41.0 35.5 41.0 24-25 39.1156930569102 40.0 39.0 41.0 36.0 41.0 26-27 39.05396892710247 40.0 39.0 41.0 35.5 41.0 28-29 38.967615259249555 40.0 39.0 41.0 35.0 41.0 30-31 38.87011576176012 40.0 38.0 41.0 35.0 41.0 32-33 38.7507660417893 40.0 38.0 41.0 35.0 41.0 34-35 38.66350192728481 40.0 38.0 41.0 35.0 41.0 36-37 38.543836627620244 40.0 38.0 41.0 34.0 41.0 38-39 38.19346700700731 40.0 37.0 41.0 34.0 41.0 40-41 38.06025231690259 40.0 37.0 41.0 33.5 41.0 42-43 37.89663822344694 40.0 37.0 41.0 33.0 41.0 44-45 37.767496870973034 40.0 36.5 41.0 33.0 41.0 46-47 37.61164604945622 40.0 36.0 41.0 33.0 41.0 48-49 37.65827998588381 40.0 36.0 41.0 33.0 41.0 50-51 37.66612062303356 40.0 36.0 41.0 33.0 41.0 52-53 37.43919115455971 40.0 36.0 41.0 32.5 41.0 54-55 36.97754665011314 40.0 35.5 41.0 31.0 41.0 56-57 36.55183671817923 39.0 35.0 41.0 30.0 41.0 58-59 36.430445099688306 39.0 35.0 41.0 30.0 41.0 60-61 36.28642648981762 39.0 35.0 41.0 30.0 41.0 62-63 36.05736186772392 39.0 35.0 41.0 29.0 41.0 64-65 35.80283018997806 38.0 35.0 40.5 29.0 41.0 66-67 35.536003199240774 37.5 35.0 40.0 29.0 41.0 68-69 35.27887806600282 37.0 35.0 40.0 29.0 41.0 70-71 34.99086623948935 36.5 34.5 39.0 29.0 41.0 72-73 34.6666839779134 36.0 34.0 39.0 28.0 41.0 74-75 34.307866347792995 35.5 34.0 39.0 28.0 40.5 76-77 32.61692958090772 34.5 32.0 36.5 25.5 39.0 78-79 33.58091358944596 35.0 34.0 37.0 27.0 39.0 80-81 33.49931971771909 35.0 34.0 37.0 27.0 39.0 82-83 33.256282892643554 35.0 34.0 36.0 27.0 38.5 84-85 33.01666577815864 35.0 34.0 36.0 27.0 37.0 86-87 32.80630678675318 35.0 34.0 35.5 26.5 37.0 88-89 32.63832532579818 35.0 34.0 35.0 26.0 36.5 90-91 32.481409250722336 35.0 34.0 35.0 26.0 36.0 92-93 32.34325160053821 35.0 33.0 35.0 26.0 36.0 94-95 32.24155130212189 35.0 33.0 35.0 25.0 36.0 96-97 32.147939435162826 35.0 33.0 35.0 25.0 35.5 98-99 32.06249627132573 35.0 33.0 35.0 25.0 35.0 100 32.01211562913405 35.0 33.0 35.0 25.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.597868869103639 1101 2 1.1891758418605107 1101 3 1.5557197557049243 1101 4 0.9666076592318049 1101 5 0.5935588242787588 1101 6 1.1139786972716266 1101 7 0.8199840042971118 1101 8 0.4967662454076276 1101 9 0.5126101372076874 1101 10-11 0.4262042467613014 1101 12-13 0.3896327869822258 1101 14-15 0.5211089270629188 1101 16-17 0.544692448934299 1101 18-19 0.515039451809912 1101 20-21 0.5051189429229623 1101 22-23 0.4721781270848808 1101 24-25 0.5094640445212733 1101 26-27 0.5106106762000593 1101 28-29 0.510007956693812 1101 30-31 0.4713177818963956 1101 32-33 0.5366439834158712 1101 34-35 0.5528574284127146 1101 36-37 0.5231379219716459 1101 38-39 0.5207097805498435 1101 40-41 0.5126876137220151 1101 42-43 0.520001772357432 1101 44-45 0.4405942820922917 1101 46-47 0.49890749686682767 1101 48-49 0.4335609552380717 1101 50-51 0.4262065024317536 1101 52-53 0.4247731935529018 1101 54-55 0.39953256337129517 1101 56-57 0.4119560792512402 1101 58-59 0.395895075090138 1101 60-61 0.3954325750922649 1101 62-63 0.3401517990126379 1101 64-65 0.3199883040769862 1101 66-67 0.3051049709352114 1101 68-69 0.29319168387927874 1101 70-71 0.29999601287115496 1101 72-73 0.28296311542509045 1101 74-75 0.3130554326833206 1101 76-77 0.22319391507054576 1101 78-79 0.32376099770346656 1101 80-81 0.32074064973794947 1101 82-83 0.3002106505739235 1101 84-85 0.2983238382151896 1101 86-87 0.3395015617976114 1101 88-89 0.3197731751812043 1101 90-91 0.30996369459564477 1101 92-93 0.30482008089848023 1101 94-95 0.2539942535608688 1101 96-97 0.23152082296099508 1101 98-99 0.26364176617125423 1101 100 0.24975239854826725 1102 1 1.478196270861499 1102 2 1.1156575773150479 1102 3 1.465742389881168 1102 4 0.8872845906814391 1102 5 0.48990867805863303 1102 6 1.0633353516520643 1102 7 0.7474517718876967 1102 8 0.4151892174867058 1102 9 0.41024483107536724 1102 10-11 0.332055796939315 1102 12-13 0.33701385228762604 1102 14-15 0.4336889801774575 1102 16-17 0.43760627383677786 1102 18-19 0.37972206993905644 1102 20-21 0.381207066693932 1102 22-23 0.4004524573710171 1102 24-25 0.3838062959929758 1102 26-27 0.39689694239910267 1102 28-29 0.3958298160555813 1102 30-31 0.36844227846166433 1102 32-33 0.38742815528028274 1102 34-35 0.3836749121647145 1102 36-37 0.36463015324878967 1102 38-39 0.4143582245749897 1102 40-41 0.3882489509448206 1102 42-43 0.37791297757947007 1102 44-45 0.38053791144376703 1102 46-47 0.4108198963013976 1102 48-49 0.35530338578720233 1102 50-51 0.30757477520604937 1102 52-53 0.322547853489489 1102 54-55 0.37722800174357474 1102 56-57 0.4101960273227334 1102 58-59 0.45950552063497696 1102 60-61 0.4964313476760438 1102 62-63 0.42155514367316016 1102 64-65 0.3784110719539058 1102 66-67 0.4006048522981658 1102 68-69 0.3462911050235036 1102 70-71 0.331496649747244 1102 72-73 0.2904218726582002 1102 74-75 0.32001603875890083 1102 76-77 0.13419194593424066 1102 78-79 0.3357832566616281 1102 80-81 0.34609014698177276 1102 82-83 0.32472056843444363 1102 84-85 0.32759821226196806 1102 86-87 0.2918139736982468 1102 88-89 0.32084953741721023 1102 90-91 0.3303120283735268 1102 92-93 0.2898523055325981 1102 94-95 0.28222735741066174 1102 96-97 0.2894588422640041 1102 98-99 0.3024502448442732 1102 100 0.3162152070705275 1103 1 1.4499319083611262 1103 2 1.064700968422187 1103 3 1.4194553935015755 1103 4 0.8813551145577208 1103 5 0.49398703795997534 1103 6 1.0358964818375327 1103 7 0.7481801909140273 1103 8 0.43081078145296914 1103 9 0.4239597387650704 1103 10-11 0.3341090515609295 1103 12-13 0.3191080328104263 1103 14-15 0.36616739503269713 1103 16-17 0.3841533606388623 1103 18-19 0.3726732777058288 1103 20-21 0.3654035817158956 1103 22-23 0.35415744080312095 1103 24-25 0.3616022723100727 1103 26-27 0.39325538394783877 1103 28-29 0.37110500088726184 1103 30-31 0.36070448501316577 1103 32-33 0.4011861711410347 1103 34-35 0.4312788895035382 1103 36-37 0.35270165449831126 1103 38-39 0.37365214313299333 1103 40-41 0.3842304372756544 1103 42-43 0.41701092803467077 1103 44-45 0.39438191442040704 1103 46-47 0.39585993374182493 1103 48-49 0.38075258214404784 1103 50-51 0.35495767478521856 1103 52-53 0.3471173806383874 1103 54-55 0.3937639917960212 1103 56-57 0.4420427387816446 1103 58-59 0.42845945628819493 1103 60-61 0.407448751033499 1103 62-63 0.3286462859936279 1103 64-65 0.317899292394884 1103 66-67 0.36830152678607675 1103 68-69 0.3042386880235526 1103 70-71 0.29104668219884644 1103 72-73 0.28286618123740936 1103 74-75 0.3048612330150391 1103 76-77 0.15491092036790377 1103 78-79 0.30796496198973244 1103 80-81 0.3188088920145802 1103 82-83 0.2983180348430423 1103 84-85 0.2659329318710988 1103 86-87 0.2702979115260362 1103 88-89 0.2691873056802976 1103 90-91 0.2693176612491257 1103 92-93 0.2884677337700907 1103 94-95 0.2811193907272127 1103 96-97 0.2820295036023239 1103 98-99 0.3213659549355796 1103 100 0.30479104599573503 1104 1 1.4119082389745579 1104 2 1.032324123909241 1104 3 1.377644128051461 1104 4 0.8540940504617964 1104 5 0.4656609306387267 1104 6 1.007138717643123 1104 7 0.7296525875113034 1104 8 0.4023420034767611 1104 9 0.4055176750361724 1104 10-11 0.2908011194282665 1104 12-13 0.25527640190230727 1104 14-15 0.32205846903104884 1104 16-17 0.3284100390893059 1104 18-19 0.31352925845193624 1104 20-21 0.3185928764540833 1104 22-23 0.3318469658371228 1104 24-25 0.317010940042465 1104 26-27 0.2844252963421994 1104 28-29 0.25034212963998925 1104 30-31 0.27236777678380975 1104 32-33 0.30442708151608144 1104 34-35 0.3234072396960386 1104 36-37 0.29759093274100934 1104 38-39 0.3087821177858885 1104 40-41 0.3052862772361209 1104 42-43 0.3225306343760508 1104 44-45 0.2868213139218554 1104 46-47 0.2899349682656336 1104 48-49 0.250781619570553 1104 50-51 0.25619777471225547 1104 52-53 0.30195415420876515 1104 54-55 0.281124863659457 1104 56-57 0.328783179397071 1104 58-59 0.3117651003712467 1104 60-61 0.3124287693276173 1104 62-63 0.28504911488114004 1104 64-65 0.26936206245868277 1104 66-67 0.2636884866649041 1104 68-69 0.24843730413054033 1104 70-71 0.26037254964503376 1104 72-73 0.23616101894713637 1104 74-75 0.22833330785962147 1104 76-77 0.1409629677044819 1104 78-79 0.2551467255968518 1104 80-81 0.2773723229634797 1104 82-83 0.3187587584041154 1104 84-85 0.30634168305766707 1104 86-87 0.2952981257308025 1104 88-89 0.252289662587323 1104 90-91 0.25828859979741736 1104 92-93 0.26018380079650427 1104 94-95 0.26064545171853837 1104 96-97 0.2318262155694768 1104 98-99 0.2554463013948194 1104 100 0.2532700842088005 1105 1 1.3716868827332043 1105 2 1.0052080229544913 1105 3 1.3492320310155002 1105 4 0.829189858068176 1105 5 0.44165570678175925 1105 6 0.9932597683143243 1105 7 0.7151121744368893 1105 8 0.363620820085508 1105 9 0.35600054202602394 1105 10-11 0.24120248753102658 1105 12-13 0.24306654038574038 1105 14-15 0.31842273511055197 1105 16-17 0.3048937828140055 1105 18-19 0.29131817810404925 1105 20-21 0.30406874127731953 1105 22-23 0.29826868752194713 1105 24-25 0.3027851611294494 1105 26-27 0.3080624382895891 1105 28-29 0.29444890968843396 1105 30-31 0.2999791164454564 1105 32-33 0.3373608175056404 1105 34-35 0.34007086786471774 1105 36-37 0.327770811393286 1105 38-39 0.3519229836683664 1105 40-41 0.3706026752922682 1105 42-43 0.35327488212288216 1105 44-45 0.3136338814756172 1105 46-47 0.353944546194775 1105 48-49 0.35805137564211975 1105 50-51 0.3420560333819793 1105 52-53 0.35983830036659015 1105 54-55 0.370801410667859 1105 56-57 0.41313041221238933 1105 58-59 0.385204465379438 1105 60-61 0.36788203235914096 1105 62-63 0.3463526652711195 1105 64-65 0.3179659246481066 1105 66-67 0.34569360060825716 1105 68-69 0.27153902905356375 1105 70-71 0.2921407830140268 1105 72-73 0.2749509080013155 1105 74-75 0.318847347715284 1105 76-77 0.2088576058505751 1105 78-79 0.31597482559198653 1105 80-81 0.29565605080603774 1105 82-83 0.30665799323030996 1105 84-85 0.2936198319476304 1105 86-87 0.3276052704504835 1105 88-89 0.3195860083585629 1105 90-91 0.30258335915738854 1105 92-93 0.29829283677687357 1105 94-95 0.30191070416334753 1105 96-97 0.3197831357384686 1105 98-99 0.33874486950612237 1105 100 0.3058986220050528 1106 1 1.314304637223703 1106 2 0.9713510648799897 1106 3 1.3088267453490339 1106 4 0.7717453652602018 1106 5 0.40768675527799303 1106 6 0.9259826082895728 1106 7 0.6579881464771944 1106 8 0.3035960254337624 1106 9 0.28046932330708074 1106 10-11 0.20841752891895737 1106 12-13 0.18653891697829295 1106 14-15 0.24878301560001148 1106 16-17 0.251892801165134 1106 18-19 0.21209436538588022 1106 20-21 0.23664078401630206 1106 22-23 0.23954353220877778 1106 24-25 0.251084220316514 1106 26-27 0.23540118046553715 1106 28-29 0.24848690213011082 1106 30-31 0.2633278356238762 1106 32-33 0.27482075749857415 1106 34-35 0.28680213158299495 1106 36-37 0.2663693248894745 1106 38-39 0.2825622485500645 1106 40-41 0.27329294117866 1106 42-43 0.3007036456027592 1106 44-45 0.28236340435002916 1106 46-47 0.3060985837702148 1106 48-49 0.2754595156627886 1106 50-51 0.2658968408603215 1106 52-53 0.25424596170507385 1106 54-55 0.25663880828999197 1106 56-57 0.27434687109281697 1106 58-59 0.2630452219041146 1106 60-61 0.24935233106902643 1106 62-63 0.2407371294915137 1106 64-65 0.21389106501584365 1106 66-67 0.1896008484166387 1106 68-69 0.21257925784768616 1106 70-71 0.20084262927650798 1106 72-73 0.1597940886696989 1106 74-75 0.1989135736700618 1106 76-77 0.060475497847839677 1106 78-79 0.20287397841065768 1106 80-81 0.2332776254222182 1106 82-83 0.24812697405555184 1106 84-85 0.25569342441773557 1106 86-87 0.24390817037342316 1106 88-89 0.22160565655636333 1106 90-91 0.2157618294651087 1106 92-93 0.21530971306125224 1106 94-95 0.17241811907735638 1106 96-97 0.1987949688023889 1106 98-99 0.22415621912099226 1106 100 0.21223650658264148 1107 1 1.2479159880764747 1107 2 0.9066175908663823 1107 3 1.2591902956698355 1107 4 0.7681600389400316 1107 5 0.35513311487490995 1107 6 0.9220971637254323 1107 7 0.6427601239031873 1107 8 0.2750548429218398 1107 9 0.26685128241280864 1107 10-11 0.16565814427080028 1107 12-13 0.1151246982858325 1107 14-15 0.17221455529853102 1107 16-17 0.16923988691674197 1107 18-19 0.12365988377008819 1107 20-21 0.1378717346250653 1107 22-23 0.147130819132542 1107 24-25 0.17164101160857825 1107 26-27 0.14006832415637405 1107 28-29 0.14294481635395329 1107 30-31 0.15026483934024526 1107 32-33 0.17000898343258086 1107 34-35 0.16855416251434718 1107 36-37 0.1659331914337443 1107 38-39 0.139079400436124 1107 40-41 0.1500858325848995 1107 42-43 0.18033085180745445 1107 44-45 0.15868202749312843 1107 46-47 0.19575756863230254 1107 48-49 0.1789857489478166 1107 50-51 0.1342993372290735 1107 52-53 0.12941837425866254 1107 54-55 0.15208119795471475 1107 56-57 0.17750862788648902 1107 58-59 0.13943463001815104 1107 60-61 0.14362446511129434 1107 62-63 0.1146312511070775 1107 64-65 0.08923119728189022 1107 66-67 0.09792288441366992 1107 68-69 0.08426272200234308 1107 70-71 0.0848801078758683 1107 72-73 0.08715992405763018 1107 74-75 0.0994469345383564 1107 76-77 -0.04918502128700908 1107 78-79 0.13092270620705193 1107 80-81 0.18154113059342336 1107 82-83 0.18129598387400137 1107 84-85 0.18254271834942415 1107 86-87 0.1409446117850237 1107 88-89 0.14511646323965266 1107 90-91 0.16023477597307334 1107 92-93 0.13933086953135643 1107 94-95 0.10140120490697058 1107 96-97 0.1910156026766643 1107 98-99 0.19924986116997445 1107 100 0.21977204490915625 1108 1 1.2179259480755924 1108 2 0.87420581261987 1108 3 1.2195761551961688 1108 4 0.7233274420223026 1108 5 0.3325218871828781 1108 6 0.8717345180415421 1108 7 0.605510949107412 1108 8 0.2614056810087746 1108 9 0.19603928959300987 1108 10-11 0.10747172312723308 1108 12-13 0.10222984783349176 1108 14-15 0.12453114792520381 1108 16-17 0.14238970738756507 1108 18-19 0.0859521540203474 1108 20-21 0.10413752932819165 1108 22-23 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-0.7074831564688324 2316 92-93 -0.7984218851716918 2316 94-95 -0.8244360295725777 2316 96-97 -0.9561396840797585 2316 98-99 -1.0837131473370682 2316 100 -0.97439695087456 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 63.0 7 829.0 8 1030.0 9 380.0 10 345.0 11 633.0 12 910.0 13 1259.0 14 2061.0 15 4639.0 16 9249.0 17 14483.0 18 20374.0 19 29489.0 20 43729.0 21 64882.0 22 99666.0 23 167420.0 24 291900.0 25 396218.0 26 398358.0 27 336498.0 28 306157.0 29 324121.0 30 379276.0 31 463392.0 32 577554.0 33 747720.0 34 1040818.0 35 1665306.0 36 3019829.0 37 5775596.0 38 7602773.0 39 2354085.0 40 7662.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 15.921281500144909 31.620102612808303 27.56756012149677 24.891055765550018 2 21.180797018099724 31.89454962187946 29.06621522054944 17.858438139471378 3 24.033684254396835 26.366095640942994 27.204990052164007 22.395230052496167 4 19.244462782464456 28.317767156292568 25.892187102211384 26.545582959031595 5 22.003090019771538 31.69907873049138 26.5867710440672 19.71106020566988 6 27.573441515906367 27.235242213110883 26.145394988537053 19.0459212824457 7 20.9766835690387 26.62431091491499 31.785332455514524 20.613673060531788 8 25.405604578750236 26.512569305760685 27.07966233017268 21.002163785316394 9 25.0801645848299 24.9971126676106 28.710948733826346 21.211774013733148 10-11 20.694085275173833 29.170473900240275 29.29141495391987 20.844025870666023 12-13 20.724181970930566 26.73424464937153 28.941988864916596 23.599584514781306 14-15 20.72677100937775 31.967458119530512 26.35883407453004 20.946936796561697 16-17 23.524672578790785 26.521150930837067 26.375615946118614 23.578560544253534 18-19 20.780989757656823 29.18208680629067 26.440289354302227 23.596634081750285 20-21 23.444469266231167 29.253811390874933 26.428758286263136 20.87296105663076 22-23 23.408964273162443 26.612824581269656 29.072460226497004 20.905750919070897 24-25 20.87490557319048 29.040296254948505 26.323045181954384 23.761752989906633 26-27 20.974247330795833 26.40966774419621 31.684351727823877 20.931733197184084 28-29 20.949517169573276 26.5097562755411 31.648724438242944 20.892002116642676 30-31 20.8919066880811 26.422839326338398 31.677432238491182 21.007821747089316 32-33 21.046693454207162 26.5237672725562 31.4967583671859 20.932780906050738 34-35 21.06056766037929 26.641516555057493 31.37111964440856 20.926796140154657 36-37 20.969610564255035 26.59596617522476 31.436519190496714 20.99790407002348 38-39 20.98869103941402 26.582585771030676 31.4687234282031 20.959999761352204 40-41 20.99232098400819 26.600342555120964 31.450670414173644 20.9566660466972 42-43 20.973866134080886 26.625570509115516 31.41127282387886 20.98929053292474 44-45 21.009127076207225 26.66435574489421 31.397802452646488 20.92871472625208 46-47 21.04823228018023 26.755489011132262 31.30254568570694 20.89373302298057 48-49 21.05195531405929 26.746003179872613 31.22542050980341 20.976620996264693 50-51 21.03695394005379 26.796370651262656 31.188673651633803 20.978001757049746 52-53 21.102763698732947 26.913130720730404 30.987474837908074 20.99663074262858 54-55 21.373233481394326 27.052915995900978 30.397109736788234 21.176740785916458 56-57 21.766969868741555 27.482775310160317 29.421171394045736 21.32908342705239 58-59 21.808352900141596 27.970347445086176 28.830907701898884 21.39039195287335 60-61 21.817389699519104 28.195775882925002 28.522746680156096 21.464087737399797 62-63 21.77118982034752 28.345240144249257 28.415228755153493 21.468341280249735 64-65 21.756878028788112 28.410094436025812 28.373645819497874 21.459381715688203 66-67 21.698811710281483 28.29088931331262 28.516753221622555 21.49354575478335 68-69 21.659085851488364 28.17878268112151 28.717583272138892 21.44454819525123 70-71 21.601709604357715 28.10949015551306 28.835956443056787 21.452843797072436 72-73 21.563217638615384 28.05865799791031 28.867461921353005 21.51066244212131 74-75 21.641222268619565 28.10578785317585 28.763105216257056 21.48988466194753 76-77 21.711877654550648 28.21934902575499 28.59110761072017 21.477665708974193 78-79 21.795994443491253 28.35345419882671 28.295134503969404 21.555416853712632 80-81 21.881239349307215 28.503600358400107 28.050241520606995 21.564918771685686 82-83 21.83746329745703 28.66751621133204 27.827623636497933 21.667396854712997 84-85 21.84455657051111 28.714718005952296 27.679486680607113 21.761238742929482 86-87 21.882978240000458 28.77504112316094 27.567503447457774 21.774477189380825 88-89 21.911920807953187 28.838077369538183 27.42496636299623 21.825035459512403 90-91 21.962354242678973 28.82934087254882 27.296349199379282 21.911955685392922 92-93 21.950223892337277 28.890587857972207 27.250125356212358 21.909062893478158 94-95 21.9704297606689 28.945103121698935 27.150433216936108 21.934033900696058 96-97 22.026003007522828 28.91296046773228 27.05008548295694 22.01095104178796 98-99 22.076812555277748 28.915435895874058 27.023915416000094 21.9838361328481 100 22.095584679309265 28.93036720353232 26.919734122097662 22.054313995060753 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 23535.0 1 18111.5 2 10185.0 3 7063.0 4 6358.5 5 6244.5 6 6345.0 7 6375.5 8 6307.0 9 6422.0 10 7001.0 11 13399.5 12 96252.0 13 111896.0 14 49525.0 15 52145.0 16 59890.5 17 68258.0 18 76692.5 19 86017.0 20 98304.0 21 115855.0 22 140705.0 23 169087.0 24 197273.5 25 229687.5 26 266739.5 27 307888.5 28 358373.0 29 409929.0 30 453266.5 31 496713.5 32 541765.5 33 579748.5 34 612749.0 35 653027.0 36 698415.5 37 735777.0 38 770683.5 39 794212.5 40 801284.5 41 816094.5 42 836260.0 43 836010.5 44 824711.0 45 825834.0 46 827976.0 47 822684.0 48 814749.5 49 802701.0 50 789857.5 51 779216.0 52 765141.5 53 735624.0 54 696828.0 55 656985.0 56 612010.5 57 561843.0 58 508158.5 59 445491.0 60 376703.5 61 318077.0 62 265799.0 63 215752.5 64 176357.0 65 145038.0 66 117623.0 67 96385.0 68 78536.0 69 62969.5 70 50335.5 71 40735.0 72 32577.5 73 23964.0 74 16673.5 75 12201.0 76 8728.5 77 5409.0 78 3256.5 79 2112.5 80 1348.0 81 854.0 82 500.5 83 290.5 84 189.0 85 124.0 86 86.0 87 58.5 88 33.5 89 20.5 90 15.5 91 12.0 92 7.0 93 3.5 94 4.0 95 4.5 96 2.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.33437603638023516 2 0.006191511441637796 3 2.367348683896533 4 9.063546705794673E-4 5 1.529712524184755E-5 6 0.008746131357026336 7 0.007946856563139803 8 3.2123963007879856E-4 9 0.0 10-11 5.736421965692831E-5 12-13 0.0 14-15 0.0 16-17 3.250639113892604E-5 18-19 0.0 20-21 3.0785464549218193E-4 22-23 5.353993834646642E-5 24-25 5.545207900169737E-4 26-27 0.0035527573374190932 28-29 0.006256524223915648 30-31 0.005891305358766538 32-33 0.0156279255752025 34-35 0.010059771987169995 36-37 0.005204846863538629 38-39 0.005296629614989714 40-41 0.008623754355091556 42-43 0.01934895129028192 44-45 0.03465754937606086 46-47 0.03354850779602691 48-49 0.04034616782537291 50-51 0.04042647773289262 52-53 0.06267040997519419 54-55 0.03365749981337507 56-57 0.022383518510133427 58-59 0.01726663011673542 60-61 0.01827624038269736 62-63 0.025215398820530456 64-65 0.04554527826694585 66-67 0.04822992374689009 68-69 0.05136774656212407 70-71 0.061601523348920086 72-73 0.06914874251511662 74-75 0.0601999242486358 76-77 0.06023243063977472 78-79 0.05471781699008868 80-81 0.05226645267008261 82-83 0.0487309045985606 84-85 0.05063730883182585 86-87 0.0585746046916895 88-89 0.06555009380197198 90-91 0.061232480202460506 92-93 0.06566673438194107 94-95 0.07403234974857645 96-97 0.07352754461559548 98-99 0.04783411063125729 100 0.0069410705784883255 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2.6148704E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.56162105908791 #Duplication Level Percentage of deduplicated Percentage of total 1 67.16087987791309 31.271194388703115 2 18.459916428804384 17.190472670808425 3 6.535331291584932 9.128868572831314 4 2.656367258262048 4.947390626918632 5 1.3610964355934518 3.168742822948878 6 0.8484492884763455 2.370310455473301 7 0.5568757991853026 1.8150327957049956 8 0.4096600008492899 1.5259546978088212 9 0.28726382427773023 1.2037922397003682 >10 1.505549163122418 12.542483019732739 >50 0.1465725952294354 4.837543072424808 >100 0.07127697271561569 4.8287575088571 >500 3.154461181588217E-4 0.10627471324020543 >1k 3.962440205183238E-4 0.33039038177006774 >5k 1.645794903858157E-5 0.0486644394888501 >10k+ 3.291589807428279E-5 4.684127593588482 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 1159588 4.434590716235879 No Hit AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 36702 0.1403587726565722 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 5.583450713274356E-4 0.0 0.0 0.0 1.912140655230944E-5 2 5.659936339483594E-4 0.0 0.0 0.0 2.676996917323321E-5 3 5.659936339483594E-4 0.0 0.0 0.0 4.2067094415080763E-5 4 5.659936339483594E-4 1.1472843931385662E-5 0.0 0.0 4.589137572554265E-5 5 5.736421965692832E-4 1.529712524184755E-5 0.0 0.0 4.589137572554265E-5 6 5.736421965692832E-4 1.529712524184755E-5 3.824281310461887E-6 0.0 5.353993834646642E-5 7 5.736421965692832E-4 2.2945687862771325E-5 3.824281310461887E-6 0.0 5.7364219656928316E-5 8 5.851150405006688E-4 2.2945687862771325E-5 3.824281310461887E-6 7.648562620923774E-6 8.030990751969964E-5 9 5.851150405006688E-4 2.2945687862771325E-5 3.824281310461887E-6 7.648562620923774E-6 8.030990751969964E-5 10-11 5.851150405006688E-4 2.676996917323321E-5 3.824281310461887E-6 1.3384984586616606E-5 8.413418883016153E-5 12-13 5.908514624663616E-4 3.824281310461888E-5 3.824281310461887E-6 1.529712524184755E-5 9.560703276154719E-5 14-15 5.927636031215925E-4 4.397923507031171E-5 3.824281310461887E-6 1.7209265897078495E-5 9.560703276154719E-5 16-17 6.004121657425163E-4 4.9715657036004536E-5 1.529712524184755E-5 1.912140655230944E-5 9.751917341677813E-5 18-19 6.271821349157496E-4 5.162779769123548E-5 1.529712524184755E-5 1.912140655230944E-5 1.0325559538247096E-4 20-21 6.539521040889827E-4 7.266134489877587E-5 1.529712524184755E-5 1.912140655230944E-5 1.1090415800339473E-4 22-23 7.247013083325277E-4 7.457348555400681E-5 1.529712524184755E-5 1.912140655230944E-5 1.2620128324524228E-4 24-25 9.694553122020884E-4 8.222204817493058E-5 1.529712524184755E-5 2.2945687862771325E-5 1.3002556455570416E-4 26-27 0.0018260943257455515 9.751917341677813E-5 1.529712524184755E-5 2.2945687862771325E-5 1.319377052109351E-4 28-29 0.003912239780602511 1.0899201734816379E-4 1.529712524184755E-5 2.2945687862771325E-5 1.3576198652139702E-4 30-31 0.00828721759977091 1.1855272062431852E-4 1.529712524184755E-5 2.676996917323321E-5 1.434105491423208E-4 32-33 0.015522757839164802 1.2811342390047322E-4 1.529712524184755E-5 3.441853179415699E-5 1.4532268979755174E-4 34-35 0.027649553874639445 1.3576198652139702E-4 1.529712524184755E-5 3.441853179415699E-5 1.5488339307370645E-4 36-37 0.046954525929851054 1.3767412717662796E-4 1.529712524184755E-5 3.824281310461888E-5 1.567955337289374E-4 38-39 0.07721797608019121 1.4914697110801362E-4 1.529712524184755E-5 3.824281310461888E-5 1.663562370050921E-4 40-41 0.11646083874749585 1.6061981503939928E-4 1.529712524184755E-5 3.824281310461888E-5 1.7209265897078494E-4 42-43 0.16071733421281606 1.70180518315554E-4 1.529712524184755E-5 3.824281310461888E-5 1.7782908093647777E-4 44-45 0.21344078849949888 1.7782908093647777E-4 1.529712524184755E-5 3.824281310461888E-5 1.797412215917087E-4 46-47 0.2805091219817242 1.835655029021706E-4 1.912140655230944E-5 3.824281310461888E-5 1.9312620617832532E-4 48-49 0.3681367917889927 1.9121406552309437E-4 1.912140655230944E-5 4.2067094415080763E-5 1.9503834683355626E-4 50-51 0.47285708691337053 2.0459905010971097E-4 1.912140655230944E-5 4.589137572554265E-5 2.0268690945448003E-4 52-53 0.5823883279263095 2.141597533858657E-4 1.912140655230944E-5 4.589137572554265E-5 2.103354720754038E-4 54-55 0.7003444606661959 2.2754473797248232E-4 1.912140655230944E-5 4.780351638077359E-5 2.3328115993817515E-4 56-57 0.8357125462126154 2.313690192829442E-4 1.912140655230944E-5 4.9715657036004536E-5 2.3710544124863704E-4 58-59 0.9985905993658424 2.5240256649048455E-4 2.2945687862771325E-5 4.9715657036004536E-5 2.4092972255909892E-4 60-61 1.183374135865395 2.7152397304279404E-4 2.2945687862771325E-5 5.353993834646642E-5 2.4092972255909892E-4 62-63 1.3754945560590688 2.810846763189488E-4 2.2945687862771325E-5 5.736421965692831E-5 2.5240256649048455E-4 64-65 1.5740455052762845 2.8873323893987256E-4 2.2945687862771325E-5 6.11885009673902E-5 2.5813898845617736E-4 66-67 1.793280079961133 3.116789268026439E-4 2.2945687862771325E-5 6.11885009673902E-5 2.791725356637178E-4 68-69 2.0402923219445217 3.1932748942356765E-4 2.2945687862771325E-5 7.266134489877587E-5 2.8873323893987256E-4 70-71 2.315562178530913 3.2888819269972233E-4 2.2945687862771325E-5 7.266134489877587E-5 2.9064537959510347E-4 72-73 2.5975857159115803 3.40361036631108E-4 2.4857828518002268E-5 7.266134489877587E-5 2.9446966090556536E-4 74-75 2.889517966167654 3.4992173990726274E-4 2.676996917323321E-5 7.457348555400681E-5 3.097667861474129E-4 76-77 3.2007571006195947 3.594824431834174E-4 2.676996917323321E-5 8.413418883016153E-5 3.155032081131057E-4 78-79 3.541772089354792 3.633067244938793E-4 2.676996917323321E-5 8.413418883016153E-5 3.174153487683367E-4 80-81 3.915891969254002 3.766917090804959E-4 2.676996917323321E-5 8.604632948539247E-5 3.1932748942356765E-4 82-83 4.301572651554738 3.939009749775744E-4 2.676996917323321E-5 9.17827514510853E-5 3.2506391138926045E-4 84-85 4.696597965237588 4.111102408746529E-4 2.676996917323321E-5 9.943131407200907E-5 3.346246146654152E-4 86-87 5.110966493788755 4.5508947594496464E-4 2.676996917323321E-5 9.943131407200907E-5 3.38448895975877E-4 88 5.438078307819768 4.589137572554265E-4 2.676996917323321E-5 9.943131407200907E-5 3.5183388056249365E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 160120 0.0 81.83696 8 AAGCAGT 156380 0.0 81.16992 1 AGTGGTA 160765 0.0 80.9458 5 GTGGTAT 161285 0.0 80.62662 6 TGGTATC 162880 0.0 80.44228 7 GTATCAA 163015 0.0 80.334496 9 GCAGTGG 164265 0.0 79.30495 3 AGCAGTG 168405 0.0 77.05466 2 CAGTGGT 169180 0.0 76.743 4 CAACGCA 157290 0.0 43.26314 12-13 ATCAACG 158390 0.0 43.03381 10-11 ACGCAGA 159625 0.0 42.715645 14-15 AGAGTAC 160805 0.0 42.323746 18-19 GAGTACT 155600 0.0 41.776676 20-21 TCAACGC 156890 0.0 41.62169 12-13 AACGCAG 158065 0.0 41.42373 14-15 AGTACTT 164115 0.0 41.229465 20-21 CGCAGAG 161245 0.0 40.59086 16-17 TATCAAC 163485 0.0 39.98287 10-11 GTACTTT 164750 0.0 39.441128 22-23 >>END_MODULE