FastQCFastQC Report
Thu 26 May 2016
SRR1660720_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660720_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27848634
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT15959595.730834050962787No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA336690.12090000536471555No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1998950.085.8589868
GTGGTAT2018250.085.085036
GTATCAA2022350.084.761189
AGTGGTA2026500.084.726085
TGGTATC2025550.084.503757
GCAGTGG2100000.082.285023
AAGCAGT2114250.081.649281
CAGTGGT2157600.079.608474
AGCAGTG2184950.079.170452
CAACGCA1976300.044.46601512-13
ATCAACG1983750.044.37657510-11
ACGCAGA1992300.044.0839414-15
TCAACGC1972700.043.67261512-13
AGAGTAC1982350.043.6598918-19
GAGTACT1933800.043.6120120-21
AACGCAG1985150.043.42685314-15
CGCAGAG2000000.043.02117516-17
TATCAAC2018500.042.48205610-11
AGTACTT2045550.042.06704720-21
GTACTTT2035450.041.426422-23
Produced by FastQC (version 0.11.5)