##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660720_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 27848634 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.82863662181779 34.0 31.0 34.0 30.0 34.0 2 31.950990809818535 34.0 31.0 34.0 30.0 34.0 3 32.01264108681237 34.0 31.0 34.0 30.0 34.0 4 35.40575860920144 37.0 35.0 37.0 33.0 37.0 5 35.37152859993061 37.0 35.0 37.0 33.0 37.0 6 35.45401444106738 37.0 35.0 37.0 33.0 37.0 7 35.405234705587354 37.0 35.0 37.0 33.0 37.0 8 35.41531911403626 37.0 35.0 37.0 33.0 37.0 9 37.09397599178473 39.0 37.0 39.0 33.0 39.0 10-11 37.02510595313221 39.0 37.0 39.0 33.0 39.0 12-13 37.05357287901446 39.0 37.0 39.0 33.0 39.0 14-15 38.43290775052019 40.0 38.0 41.0 33.5 41.0 16-17 38.409093781763225 40.0 38.0 41.0 33.0 41.0 18-19 38.37177302843651 40.0 38.0 41.0 33.5 41.0 20-21 38.251161995952835 40.0 38.0 41.0 33.5 41.0 22-23 38.075990136535964 40.0 38.0 41.0 33.0 41.0 24-25 38.061298374634816 40.0 38.0 41.0 33.0 41.0 26-27 38.04897549014433 40.0 38.0 41.0 33.0 41.0 28-29 37.970559381835386 40.0 38.0 41.0 33.0 41.0 30-31 37.882755003351335 40.0 38.0 41.0 32.0 41.0 32-33 37.75980408949322 40.0 38.0 41.0 32.0 41.0 34-35 37.60817406699374 40.0 38.0 41.0 31.5 41.0 36-37 37.423671983336774 40.0 37.0 41.0 31.0 41.0 38-39 36.99766801847444 40.0 35.0 41.0 30.5 41.0 40-41 36.93929689693218 39.5 35.0 41.0 30.5 41.0 42-43 36.95161795009407 40.0 35.0 41.0 30.0 41.0 44-45 36.95544368531685 40.0 35.0 41.0 30.0 41.0 46-47 37.05001358414923 40.0 35.0 41.0 31.0 41.0 48-49 36.946268621290365 40.0 35.0 41.0 30.0 41.0 50-51 35.59115535074359 38.5 34.0 40.0 28.0 40.5 52-53 35.750715816079165 38.5 35.0 39.5 28.5 40.5 54-55 36.117227096309286 39.0 35.0 41.0 27.0 41.0 56-57 35.72545985558933 39.0 35.0 41.0 24.5 41.0 58-59 35.53504184442224 39.0 35.0 41.0 24.0 41.0 60-61 35.3161676260315 39.0 35.0 41.0 24.0 41.0 62-63 35.096132219627 38.5 35.0 41.0 23.5 41.0 64-65 34.851932899114544 38.0 34.5 40.5 23.0 41.0 66-67 34.597744201744334 37.0 34.0 40.0 23.0 41.0 68-69 34.34114012558031 37.0 34.0 40.0 23.0 41.0 70-71 34.05871767354908 36.0 34.0 39.0 24.0 41.0 72-73 33.725539446566756 36.0 34.0 39.0 23.0 41.0 74-75 33.35113093877423 35.0 33.0 38.5 22.5 40.5 76-77 32.960346852201084 35.0 33.0 37.0 21.0 39.5 78-79 32.587167937931895 35.0 33.0 37.0 20.0 39.0 80-81 32.2633926317535 35.0 33.0 36.5 20.0 39.0 82-83 31.942868634059394 35.0 32.5 36.0 19.5 38.0 84-85 31.657652831374065 35.0 32.0 36.0 19.0 37.0 86-87 31.444033736089175 35.0 32.0 35.0 18.5 37.0 88-89 31.24141704760097 35.0 32.0 35.0 18.0 36.0 90-91 31.071726157196792 35.0 32.0 35.0 18.0 36.0 92-93 30.92289813927678 35.0 31.5 35.0 17.5 36.0 94-95 30.74464393837055 35.0 31.0 35.0 17.0 36.0 96-97 30.610367460034126 35.0 31.0 35.0 15.5 35.0 98-99 30.51127565179678 35.0 31.0 35.0 15.0 35.0 100 30.434444504531175 35.0 31.0 35.0 15.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.48332668408547974 1101 2 0.5339965390102748 1101 3 0.5241695773840149 1101 4 0.43765326902025237 1101 5 0.4273658665184854 1101 6 0.42747091923046554 1101 7 0.4705891237010178 1101 8 0.47281077588421283 1101 9 0.554878765749379 1101 10-11 0.5698248679221081 1101 12-13 0.5409369564665312 1101 14-15 0.6723236843289655 1101 16-17 0.6410807730370678 1101 18-19 0.6260921860102258 1101 20-21 0.689181014207854 1101 22-23 0.7020419202452644 1101 24-25 0.7071386468360501 1101 26-27 0.6768675221045655 1101 28-29 0.6715698768810512 1101 30-31 0.6578688204130643 1101 32-33 0.6636035658295754 1101 34-35 0.6941924142323259 1101 36-37 0.6233676179257728 1101 38-39 0.6582151372564908 1101 40-41 0.6408068103757074 1101 42-43 0.639487013918405 1101 44-45 0.6711436864567162 1101 46-47 0.7080473781224157 1101 48-49 0.7552434938613644 1101 50-51 0.6688215451357209 1101 52-53 0.7002136430142372 1101 54-55 0.7597813781391451 1101 56-57 0.7570894075074435 1101 58-59 0.681622703951561 1101 60-61 0.7038722836044542 1101 62-63 0.6867782782749501 1101 64-65 0.6360238868847503 1101 66-67 0.6339218816804291 1101 68-69 0.6125633964264594 1101 70-71 0.5960183564503438 1101 72-73 0.5813388700458688 1101 74-75 0.5482184341946024 1101 76-77 0.5083795950493624 1101 78-79 0.5273212629521353 1101 80-81 0.48757298004900207 1101 82-83 0.4543368253081148 1101 84-85 0.4266401926029282 1101 86-87 0.3838323322651185 1101 88-89 0.3646838693493244 1101 90-91 0.38006316960726494 1101 92-93 0.3473112629215187 1101 94-95 0.39466967978313505 1101 96-97 0.3448634102313122 1101 98-99 0.30535619428904326 1101 100 0.276351882443457 1102 1 0.43356767423171405 1102 2 0.4718866510975772 1102 3 0.477610180597587 1102 4 0.3982120159691007 1102 5 0.4154622667581762 1102 6 0.383750690018303 1102 7 0.38165606142182895 1102 8 0.40145277144788594 1102 9 0.46746313316810983 1102 10-11 0.45752134688815715 1102 12-13 0.4697960196328239 1102 14-15 0.555202442320784 1102 16-17 0.533171875856894 1102 18-19 0.5174097178661086 1102 20-21 0.5316377417450013 1102 22-23 0.5548199689765809 1102 24-25 0.49068371988732196 1102 26-27 0.48830938731642703 1102 28-29 0.5172063491250682 1102 30-31 0.4631827788616931 1102 32-33 0.4541117604061071 1102 34-35 0.5077644674410706 1102 36-37 0.5353959027927431 1102 38-39 0.505797243678856 1102 40-41 0.5051760154484555 1102 42-43 0.5030536611653176 1102 44-45 0.5002960390969449 1102 46-47 0.5206281173556278 1102 48-49 0.5560081607638452 1102 50-51 0.5022029849450078 1102 52-53 0.5154579329223736 1102 54-55 0.5701994319241734 1102 56-57 0.5715597882667822 1102 58-59 0.45775397707605237 1102 60-61 0.4949374206061776 1102 62-63 0.4832502052769456 1102 64-65 0.5003860967189766 1102 66-67 0.45439249000742876 1102 68-69 0.42016396009454127 1102 70-71 0.4446682873295842 1102 72-73 0.4064264731827407 1102 74-75 0.3827896515717839 1102 76-77 0.3541374775792576 1102 78-79 0.4002118281687146 1102 80-81 0.34874002125668113 1102 82-83 0.32940631557137934 1102 84-85 0.31368147971697624 1102 86-87 0.2612657990633629 1102 88-89 0.29586204479389266 1102 90-91 0.2858329257307126 1102 92-93 0.27418791910981 1102 94-95 0.34304249545578 1102 96-97 0.29696376955502757 1102 98-99 0.27912836458353496 1102 100 0.28200388913210617 1103 1 0.42146789283399144 1103 2 0.44797891770115683 1103 3 0.45512252729012914 1103 4 0.41604101609447497 1103 5 0.42450803453448316 1103 6 0.4004416807087168 1103 7 0.3760891319364177 1103 8 0.4250172549114879 1103 9 0.5013334224975523 1103 10-11 0.47153229300746347 1103 12-13 0.4685746635625918 1103 14-15 0.5361588340614531 1103 16-17 0.5296344620381248 1103 18-19 0.5303295052175514 1103 20-21 0.5557076907762593 1103 22-23 0.5912798881577146 1103 24-25 0.6322849636459367 1103 26-27 0.5593666932780437 1103 28-29 0.5662538069272856 1103 30-31 0.5232385985639567 1103 32-33 0.4895398315344295 1103 34-35 0.5402952519538573 1103 36-37 0.553337562148748 1103 38-39 0.5774957472549431 1103 40-41 0.539158452304072 1103 42-43 0.5537684135023824 1103 44-45 0.5747298060971815 1103 46-47 0.5596466812201157 1103 48-49 0.6020229856774435 1103 50-51 0.5066396098279498 1103 52-53 0.5485441446911423 1103 54-55 0.6094877757804298 1103 56-57 0.6616499362968327 1103 58-59 0.5728547252812746 1103 60-61 0.5835331539409623 1103 62-63 0.5841599414899434 1103 64-65 0.5736893524839175 1103 66-67 0.5499785940256032 1103 68-69 0.5184007544071747 1103 70-71 0.523682858373931 1103 72-73 0.46711522696021035 1103 74-75 0.5045249611539759 1103 76-77 0.54871977193379 1103 78-79 0.5330259960303749 1103 80-81 0.4633056862272511 1103 82-83 0.4814617053922383 1103 84-85 0.4837688833083966 1103 86-87 0.4386362877996923 1103 88-89 0.44433126520482347 1103 90-91 0.44214200830715455 1103 92-93 0.4413266394964772 1103 94-95 0.48415479451741916 1103 96-97 0.46150756035716256 1103 98-99 0.4178034560966921 1103 100 0.44697395014664565 1104 1 0.4081292466213462 1104 2 0.4313772180241173 1104 3 0.4110617747134242 1104 4 0.3694336291469398 1104 5 0.3703089247927309 1104 6 0.3263834501911873 1104 7 0.3665165058115818 1104 8 0.37583966476601915 1104 9 0.4467964269092164 1104 10-11 0.40488805890604596 1104 12-13 0.4074780803106677 1104 14-15 0.45927029360860416 1104 16-17 0.46518189293830403 1104 18-19 0.457741206650887 1104 20-21 0.40850615260392686 1104 22-23 0.48885840218008525 1104 24-25 0.505847091965073 1104 26-27 0.4621053715202663 1104 28-29 0.45780211461555353 1104 30-31 0.4400107464280296 1104 32-33 0.4039473097031845 1104 34-35 0.3974585760925251 1104 36-37 0.397852925823436 1104 38-39 0.38308717646663837 1104 40-41 0.4021745055162569 1104 42-43 0.4293788421649438 1104 44-45 0.46549740257425753 1104 46-47 0.4666295202103612 1104 48-49 0.48282014504612647 1104 50-51 0.44635831397918935 1104 52-53 0.42853062615567694 1104 54-55 0.5406410474893306 1104 56-57 0.6314493104423562 1104 58-59 0.557922817447448 1104 60-61 0.582994700444317 1104 62-63 0.5714605555807992 1104 64-65 0.5517593078643657 1104 66-67 0.5266943383175473 1104 68-69 0.5015116869470333 1104 70-71 0.47564234452690357 1104 72-73 0.46153406403000474 1104 74-75 0.4750089976834815 1104 76-77 0.49237810138826177 1104 78-79 0.514656660716966 1104 80-81 0.4282297562629722 1104 82-83 0.43914720888936287 1104 84-85 0.40982738827095133 1104 86-87 0.3499930546689676 1104 88-89 0.3406044017717882 1104 90-91 0.3292331020251922 1104 92-93 0.35331190347255514 1104 94-95 0.4379772458277458 1104 96-97 0.4046181364774952 1104 98-99 0.387570862360171 1104 100 0.3707915522772076 1105 1 0.3028282070108794 1105 2 0.3462796472739811 1105 3 0.3525875447755169 1105 4 0.2893010723349718 1105 5 0.30224529116436827 1105 6 0.2799887401178722 1105 7 0.29912926056088196 1105 8 0.30398997661787774 1105 9 0.30878255332758187 1105 10-11 0.30756075598532817 1105 12-13 0.3177660627879604 1105 14-15 0.3759582651330362 1105 16-17 0.34194920718039157 1105 18-19 0.3459777067557468 1105 20-21 0.3865535196396621 1105 22-23 0.42022866324521857 1105 24-25 0.44897302500528014 1105 26-27 0.42490118822553313 1105 28-29 0.41853089886124195 1105 30-31 0.3732865283896203 1105 32-33 0.39926853750300495 1105 34-35 0.4219468964576194 1105 36-37 0.39814521124356617 1105 38-39 0.43171858350053327 1105 40-41 0.4396905440312864 1105 42-43 0.446048458429523 1105 44-45 0.40285087230181915 1105 46-47 0.4255489036121958 1105 48-49 0.49346003752582845 1105 50-51 0.3629337664075152 1105 52-53 0.43385610594630464 1105 54-55 0.5445919645549395 1105 56-57 0.57398419519577 1105 58-59 0.5260345039236114 1105 60-61 0.5071501750578022 1105 62-63 0.5122624843563557 1105 64-65 0.482272230539742 1105 66-67 0.4302398014422053 1105 68-69 0.4347185904821771 1105 70-71 0.46113321098516025 1105 72-73 0.4648542922411423 1105 74-75 0.437658257006035 1105 76-77 0.444897240677264 1105 78-79 0.458380878578204 1105 80-81 0.36969707657226536 1105 82-83 0.3967577051618356 1105 84-85 0.3855575370111346 1105 86-87 0.3580489683296193 1105 88-89 0.3464515229633065 1105 90-91 0.3715904369295586 1105 92-93 0.3442069917059456 1105 94-95 0.3847353890685632 1105 96-97 0.3791692173582 1105 98-99 0.3314785961637483 1105 100 0.2869618931940927 1106 1 0.17570196717599984 1106 2 0.23118108351366118 1106 3 0.20320343751524206 1106 4 0.21021968885710862 1106 5 0.20079487183415523 1106 6 0.19468913276865862 1106 7 0.2249695114957433 1106 8 0.24027423315332896 1106 9 0.20853947538339668 1106 10-11 0.22454397102693946 1106 12-13 0.22829313458225897 1106 14-15 0.29126741298499326 1106 16-17 0.25257654063316437 1106 18-19 0.2270642977669226 1106 20-21 0.2589217237405421 1106 22-23 0.25687063629302287 1106 24-25 0.2680989828137399 1106 26-27 0.2670803857764952 1106 28-29 0.23364288031101665 1106 30-31 0.2503670553628581 1106 32-33 0.2720091989171962 1106 34-35 0.27069756515233223 1106 36-37 0.2408346826271881 1106 38-39 0.2515241719902761 1106 40-41 0.2656870709561545 1106 42-43 0.29144948875596555 1106 44-45 0.3155549296723805 1106 46-47 0.3442474237682305 1106 48-49 0.3347215148238334 1106 50-51 0.24489260336886076 1106 52-53 0.280871095176046 1106 54-55 0.3960356002682417 1106 56-57 0.40878880644873306 1106 58-59 0.3541770023492319 1106 60-61 0.35473266162469486 1106 62-63 0.31646004208051437 1106 64-65 0.25916109434316326 1106 66-67 0.2650479383814215 1106 68-69 0.28156388318399905 1106 70-71 0.2650823178500019 1106 72-73 0.2547648370852187 1106 74-75 0.241836452296468 1106 76-77 0.24737159663278874 1106 78-79 0.27230108549746745 1106 80-81 0.24825599123115438 1106 82-83 0.2982477145804303 1106 84-85 0.2882919897708689 1106 86-87 0.22363232285196233 1106 88-89 0.24148248726192634 1106 90-91 0.21274842454731413 1106 92-93 0.2239927696985724 1106 94-95 0.2614999281021433 1106 96-97 0.24451324180280665 1106 98-99 0.261917668652039 1106 100 0.22006490542905865 1107 1 0.2229543426520415 1107 2 0.23349935876079542 1107 3 0.2160799949670249 1107 4 0.2287695208854288 1107 5 0.2410520511596843 1107 6 0.17479398520850253 1107 7 0.20274719638778294 1107 8 0.20345509919042115 1107 9 0.2560468818305637 1107 10-11 0.24027497156570377 1107 12-13 0.22498320485600232 1107 14-15 0.27320050031699594 1107 16-17 0.273306857655804 1107 18-19 0.2499618640076946 1107 20-21 0.2711096657742118 1107 22-23 0.27919638600111085 1107 24-25 0.29351585561112614 1107 26-27 0.2588452780633048 1107 28-29 0.28876148736603824 1107 30-31 0.24623054574971803 1107 32-33 0.18465636429067445 1107 34-35 0.24963145116847585 1107 36-37 0.21655006851386105 1107 38-39 0.22413123377666722 1107 40-41 0.24657092973929196 1107 42-43 0.29564573390914006 1107 44-45 0.3009429871496323 1107 46-47 0.28704348543026725 1107 48-49 0.3481804497401768 1107 50-51 0.2779638386130969 1107 52-53 0.27626358376674176 1107 54-55 0.34281339887760254 1107 56-57 0.40310168534650614 1107 58-59 0.29649454509442563 1107 60-61 0.3077334702980892 1107 62-63 0.2939533389557667 1107 64-65 0.26393998259055707 1107 66-67 0.27442135265598466 1107 68-69 0.2780849891464001 1107 70-71 0.3058982090907989 1107 72-73 0.27660918966979153 1107 74-75 0.2979772069233064 1107 76-77 0.2726630385743931 1107 78-79 0.2968426900146781 1107 80-81 0.2595888031575342 1107 82-83 0.2597865885505932 1107 84-85 0.2653760818351394 1107 86-87 0.23182318832222037 1107 88-89 0.2655364109574698 1107 90-91 0.2711944001476603 1107 92-93 0.2568894359118623 1107 94-95 0.26637930716360003 1107 96-97 0.2960249087377633 1107 98-99 0.28700900600709645 1107 100 0.3060284797065336 1108 1 0.09661373962035569 1108 2 0.12884668199306049 1108 3 0.1506457465428923 1108 4 0.11415629116796566 1108 5 0.1549687569385938 1108 6 0.07576978533455758 1108 7 0.07535298257446499 1108 8 0.11466110159884124 1108 9 0.12703861240724024 1108 10-11 0.08469761772803253 1108 12-13 0.11259295752017806 1108 14-15 0.14099494015705005 1108 16-17 0.12816383401227682 1108 18-19 0.11757405627235329 1108 20-21 0.1014629344847151 1108 22-23 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-1.2457292837301033 2316 86-87 -1.3968055712962553 2316 88-89 -1.3360071078472942 2316 90-91 -1.2528919413861637 2316 92-93 -1.149219931681447 2316 94-95 -1.3982271291252637 2316 96-97 -1.5236562032666008 2316 98-99 -1.4033991461183604 2316 100 -1.179122661721042 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 14649.0 3 3531.0 4 2510.0 5 2097.0 6 3783.0 7 20563.0 8 39552.0 9 45001.0 10 45602.0 11 43774.0 12 42090.0 13 44554.0 14 50673.0 15 59200.0 16 71405.0 17 85224.0 18 94281.0 19 105828.0 20 122983.0 21 148460.0 22 192268.0 23 278138.0 24 415396.0 25 523108.0 26 518417.0 27 437448.0 28 389265.0 29 376163.0 30 413937.0 31 499134.0 32 622381.0 33 804815.0 34 1110725.0 35 1698082.0 36 2969825.0 37 5730650.0 38 7423900.0 39 2394738.0 40 4484.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 15.906265299512699 34.1282911607751 28.26486981485893 21.700573724853275 2 20.44502041688108 34.092855128012175 27.019762603627722 18.44236185147902 3 25.82367310135396 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20.180933918319884 22-23 23.48084948808103 26.70183339591283 29.609799409890808 20.20751770611533 24-25 20.241348796892513 29.869831709495244 26.24357756479081 23.645241928821438 26-27 20.2740010106521 26.609012414631884 32.86757258760722 20.249413987108795 28-29 20.26019809455153 26.714070260056115 32.83816764462848 20.187564000763885 30-31 20.22243487664875 26.649578826693222 32.84192403657498 20.28606226008305 32-33 20.345634748978178 26.734753672735636 32.6601719070403 20.259439671245886 34-35 20.356982754706877 26.827814833063574 32.58543375892723 20.229768653302322 36-37 20.299555146902886 26.80960603757959 32.601339232117994 20.289499583399532 38-39 20.326867837007985 26.795103139752328 32.61081122543646 20.267217797803223 40-41 20.329863325837756 26.822546519009556 32.60171016226545 20.245879992887232 42-43 20.35473878543022 26.850790276657914 32.551295760518215 20.243175177393645 44-45 20.338150875415074 26.92438880970834 32.52572403762571 20.211736277250882 46-47 20.325946494389452 26.990671360411767 32.49156652763367 20.191815617565112 48-49 20.399471318352102 26.986303454920446 32.367813100194134 20.24641212653332 50-51 20.4024925766534 27.044151164893005 32.33225896543656 20.22109729301704 52-53 20.487167513979664 27.1673852934564 32.097224441629926 20.248222750934016 54-55 20.851538181333684 27.432922903731722 31.295595171929712 20.41994374300488 56-57 21.325146156156443 27.99378152218085 30.109924239823528 20.57114808183918 58-59 21.432042865009564 28.523199740665213 29.44720753724442 20.597549857080807 60-61 21.53565636444041 28.805821174359075 29.07223708141842 20.586285379782094 62-63 21.527634212154457 28.98170696263886 28.90471984096506 20.585938984241626 64-65 21.59433925143865 29.018125573754087 28.882782027002524 20.504753147804735 66-67 21.653390859533893 28.83074876616446 29.067116389419567 20.44874398488208 68-69 21.690344771198706 28.623061364479835 29.30272402232178 20.383869841999672 70-71 21.739424949550802 28.4916595211467 29.482538513673962 20.28637701562853 72-73 21.85900498657644 28.381020031596627 29.48567597967967 20.274299002147263 74-75 22.016797397129253 28.46189595922478 29.3168697334456 20.204436910200368 76-77 22.265203595947693 28.604003555346186 29.018314016071027 20.11247883263509 78-79 22.51032046743699 28.750012492336534 28.673566582571976 20.0661004576545 80-81 22.748089103222892 28.925986583942638 28.321284984069788 20.00463932876469 82-83 22.940704133294517 29.082537555551546 27.98414780893626 19.992610502217676 84-85 23.06297173962561 29.15388813824696 27.790577876367838 19.992562245759594 86-87 23.220439773492096 29.221670233485735 27.611123873221416 19.946766119800753 88-89 23.45894006151205 29.27312610667734 27.395869804565315 19.872064027245294 90-91 23.6500539067555 29.235296146248647 27.245257451551257 19.8693924954446 92-93 23.810174263154686 29.245707367565842 27.160631120097516 19.78348724918196 94-95 24.003699583333933 29.273414274228692 27.02289434056159 19.69999180187579 96-97 24.13625161102311 29.268152807957776 26.932958966529014 19.662636614490104 98-99 24.305915828298673 29.26765371695797 26.83362964209089 19.592800812652467 100 24.38059777634499 29.27043065456886 26.74116670955675 19.607804859529395 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 41164.0 1 28365.0 2 13207.5 3 10160.0 4 9374.5 5 9231.0 6 9392.0 7 9495.5 8 9440.0 9 9567.0 10 10106.5 11 12273.5 12 109603.5 13 137519.5 14 69651.0 15 74243.0 16 83970.0 17 93650.0 18 103068.0 19 112257.0 20 123400.0 21 140753.0 22 165045.0 23 193472.0 24 225294.0 25 262076.5 26 301589.5 27 347166.5 28 405500.0 29 464540.0 30 510726.0 31 552378.0 32 596780.0 33 635110.5 34 665164.5 35 702760.0 36 755051.0 37 798191.0 38 828229.5 39 847665.5 40 852065.5 41 861147.0 42 885936.0 43 892252.0 44 893430.0 45 908687.0 46 911842.0 47 915740.0 48 905874.0 49 879675.0 50 866374.0 51 854263.0 52 831263.5 53 794009.5 54 741767.5 55 686425.5 56 631943.5 57 574949.5 58 512007.5 59 440853.5 60 365588.0 61 299291.0 62 237896.5 63 175889.5 64 124898.5 65 88176.5 66 62267.5 67 45559.0 68 33423.5 69 24314.0 70 18003.5 71 13890.5 72 11327.0 73 9208.5 74 7596.0 75 6549.5 76 5314.5 77 3820.5 78 2892.5 79 2418.0 80 2045.5 81 1719.5 82 1504.5 83 1315.5 84 1096.5 85 936.0 86 816.5 87 707.5 88 596.5 89 537.5 90 459.5 91 386.0 92 341.5 93 286.0 94 254.0 95 202.5 96 157.0 97 139.5 98 112.5 99 76.5 100 47.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.40528020153519917 2 0.38820216460168205 3 0.16978570654488834 4 0.1760445413588329 5 0.276843022174804 6 0.10331925077545995 7 0.1373245093457726 8 0.1435366632345414 9 0.13583071974014954 10-11 0.13246251144670146 12-13 0.13433154387392932 14-15 0.14064065045344773 16-17 0.14517408645608973 18-19 0.15704540481231505 20-21 0.13837662558242533 22-23 0.10061534795566633 24-25 0.09528474538463898 26-27 0.08982128171888072 28-29 0.09938368969910696 30-31 0.10359933632651426 32-33 0.10478072281749978 34-35 0.10553839014150568 36-37 0.10131197099290398 38-39 0.10065843804044393 40-41 0.10138019696046852 42-43 0.06418268127621628 44-45 0.06743059641632693 46-47 0.08598087791307825 48-49 0.09007443596694904 50-51 0.09919337515800597 52-53 0.10422234713559021 54-55 0.1066246193619407 56-57 0.10747026227570086 58-59 0.10688854613120342 60-61 0.10471429227013433 62-63 0.10896405188132387 64-65 0.11628398003291651 66-67 0.12480683971788348 68-69 0.12984478879646305 70-71 0.12838152133422415 72-73 0.11510259354193099 74-75 0.11256566480065056 76-77 0.114668101853757 78-79 0.11339873977301723 80-81 0.10756182870585324 82-83 0.1105117040929189 84-85 0.11649404419620726 86-87 0.13024337208065576 88-89 0.13815040263734302 90-91 0.145181268136886 92-93 0.14436076110591278 94-95 0.13427588584775826 96-97 0.13048754922772873 98-99 0.1257602078435876 100 0.1197473455969151 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2.7848634E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 39.470554109770916 #Duplication Level Percentage of deduplicated Percentage of total 1 72.09896757898298 28.457862010848668 2 13.857649879509854 10.939382388069083 3 4.948704708784716 5.859843509440958 4 2.4485473864324603 3.865820884260823 5 1.4533500422345693 2.8682265741228705 6 0.9312141654253 2.2053323462522685 7 0.6888108276162384 1.903142152997598 8 0.5004246883975635 1.5801631793009026 9 0.3850971637539954 1.3679998595524248 >10 2.342994150007102 17.68148335218672 >50 0.21987754760810718 5.989997110472222 >100 0.11717410238571327 9.265511574455195 >500 0.006549921050744762 1.5459580804990003 >1k 5.833117133621093E-4 0.44415826504372774 >5k 1.817536580779533E-5 0.039955134048830734 >10k+ 3.635073160252361E-5 5.985163578448695 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 1595959 5.730834050962787 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 33669 0.12090000536471555 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 5.134901769329153E-4 7.181680796264549E-6 0.0 0.0 1.4363361592529098E-5 2 5.206718577291798E-4 7.181680796264549E-6 0.0 3.5908403981322746E-6 1.7954201990661374E-5 3 5.206718577291798E-4 7.181680796264549E-6 0.0 1.0772521194396824E-5 2.1545042388793648E-5 4 5.242626981273121E-4 7.181680796264549E-6 0.0 1.0772521194396824E-5 2.5135882786925922E-5 5 5.242626981273121E-4 1.0772521194396824E-5 0.0 1.0772521194396824E-5 2.8726723185058197E-5 6 5.278535385254444E-4 2.8726723185058197E-5 0.0 1.4363361592529098E-5 2.8726723185058197E-5 7 5.314443789235766E-4 2.8726723185058197E-5 0.0 1.4363361592529098E-5 2.8726723185058197E-5 8 5.314443789235766E-4 2.8726723185058197E-5 0.0 2.1545042388793648E-5 2.8726723185058197E-5 9 5.314443789235766E-4 3.590840398132275E-5 0.0 2.1545042388793648E-5 3.590840398132275E-5 10-11 5.314443789235766E-4 3.949924437945502E-5 0.0 2.693130298599206E-5 3.590840398132275E-5 12-13 5.440123203170396E-4 4.129466457852116E-5 0.0 3.0522143384124334E-5 3.770382418038888E-5 14-15 5.565802617105025E-4 4.3090084777587297E-5 0.0 3.949924437945502E-5 4.3090084777587297E-5 16-17 5.763298839002301E-4 4.488550497665343E-5 0.0 5.206718577291798E-5 4.488550497665343E-5 18-19 5.996703464880898E-4 4.847634537478571E-5 0.0 6.283970696731481E-5 4.668092517571957E-5 20-21 6.26601649474082E-4 5.924886656918253E-5 0.0 6.822596756451322E-5 5.206718577291798E-5 22-23 6.696917342516693E-4 6.104428676824867E-5 0.0 6.822596756451322E-5 5.565802617105026E-5 24-25 8.24097871371357E-4 6.104428676824867E-5 0.0 7.002138776357936E-5 6.104428676824867E-5 26-27 0.0013411788887024046 6.643054736544708E-5 0.0 8.258932915704233E-5 6.463512716638095E-5 28-29 0.002982192950648854 7.540764836077776E-5 0.0 8.618016955517459E-5 6.463512716638095E-5 30-31 0.0074635617675179325 9.336185035143914E-5 0.0 8.977100995330686E-5 6.822596756451323E-5 32-33 0.0169613346205778 1.1490689274023279E-4 0.0 8.977100995330686E-5 8.258932915704231E-5 34-35 0.03431586626475108 1.238839937355635E-4 0.0 8.977100995330686E-5 9.515727055050527E-5 36-37 0.0627714809997503 1.3286109473089415E-4 0.0 9.1566430152373E-5 9.874811094863755E-5 38-39 0.10465504340356514 1.4183819572622487E-4 0.0 9.336185035143914E-5 1.0054353114770369E-4 40-41 0.16088042235752031 1.43633615925291E-4 0.0 9.695269074957141E-5 1.0413437154583597E-4 42-43 0.23405277257046075 1.544061371196878E-4 0.0 9.695269074957141E-5 1.0413437154583597E-4 44-45 0.32765341380837565 1.6158781791595234E-4 0.0 1.0054353114770369E-4 1.0772521194396824E-4 46-47 0.4464617546411792 1.7056491891128306E-4 0.0 1.0054353114770369E-4 1.1131605234210052E-4 48-49 0.5918638594625503 1.8133744010567987E-4 0.0 1.0413437154583597E-4 1.1490689274023279E-4 50-51 0.7638525465916928 2.0467790269353966E-4 0.0 1.1131605234210052E-4 1.1670231293929893E-4 52-53 0.9585263679360359 2.0826874309167194E-4 0.0 1.1131605234210052E-4 1.238839937355635E-4 54-55 1.1814672848944765 2.1365500368887032E-4 0.0 1.1849773313836505E-4 1.292702543327619E-4 56-57 1.4376809289820103 2.1904126428606876E-4 0.0 1.2567941393462962E-4 1.4363361592529097E-4 58-59 1.7303164672278002 2.2263210468420104E-4 0.0 1.2747483413369574E-4 1.544061371196878E-4 60-61 2.0572247816535634 2.2801836528139945E-4 0.0 1.3645193512902643E-4 1.579969775178201E-4 62-63 2.4155314045205953 2.369954662767301E-4 0.0 1.400427755271587E-4 1.6517865831408462E-4 64-65 2.8016419045903653 2.4956340767019313E-4 0.0 1.43633615925291E-4 1.777465997075476E-4 66-67 3.221807934996022 2.6033592886458995E-4 0.0 1.4542903612435712E-4 1.8133744010567987E-4 68-69 3.6787836703229324 2.657221894617883E-4 0.0 1.5081529672155553E-4 1.83132860304746E-4 70-71 4.170346380364652 2.7110845005898676E-4 0.0 1.579969775178201E-4 1.885191209019444E-4 72-73 4.6916484305837045 2.7829013085525127E-4 0.0 1.579969775178201E-4 1.9570080169820894E-4 74-75 5.243659347887584 2.836763914524497E-4 0.0 1.6158781791595237E-4 1.974962218972751E-4 76-77 5.823984041730736 2.87267231850582E-4 0.0 1.6158781791595237E-4 2.0108706229540738E-4 78-79 6.437335490135711 2.9444891264684655E-4 0.0 1.633832381150185E-4 2.0467790269353966E-4 80-81 7.081857228616672 3.034260136421772E-4 0.0 1.687694987122169E-4 2.1006416329073807E-4 82-83 7.753942257993696 3.088122742393756E-4 0.0 1.741557593094153E-4 2.2263210468420104E-4 84-85 8.452590529215904 3.195847954337725E-4 0.0 1.7595117950848147E-4 2.262229450823333E-4 86-87 9.177125168868248 3.321527368272354E-4 0.0 1.7954201990661372E-4 2.316092056795317E-4 88 9.735263855311539 3.4472067822069837E-4 0.0 1.7954201990661372E-4 2.3340462587859785E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 199895 0.0 85.858986 8 GTGGTAT 201825 0.0 85.08503 6 GTATCAA 202235 0.0 84.76118 9 AGTGGTA 202650 0.0 84.72608 5 TGGTATC 202555 0.0 84.50375 7 GCAGTGG 210000 0.0 82.28502 3 AAGCAGT 211425 0.0 81.64928 1 CAGTGGT 215760 0.0 79.60847 4 AGCAGTG 218495 0.0 79.17045 2 CAACGCA 197630 0.0 44.466015 12-13 ATCAACG 198375 0.0 44.376575 10-11 ACGCAGA 199230 0.0 44.08394 14-15 TCAACGC 197270 0.0 43.672615 12-13 AGAGTAC 198235 0.0 43.65989 18-19 GAGTACT 193380 0.0 43.61201 20-21 AACGCAG 198515 0.0 43.426853 14-15 CGCAGAG 200000 0.0 43.021175 16-17 TATCAAC 201850 0.0 42.482056 10-11 AGTACTT 204555 0.0 42.067047 20-21 GTACTTT 203545 0.0 41.4264 22-23 >>END_MODULE